JOAN ZUGAY-MURPHY

*** ******* **** 267-***-****

Lansdale, PA 19446 b3hruf@r.postjobfree.com

SUMMARY

RESEARCH ASSOCIATE with extensive experience in basic research and drug discovery at global pharmaceutical firms. Expertise in expression, purification and characterization of tagged, untagged and secreted recombinant proteins, and

development of numerous protein purification protocols. Member of Merck’s HIV drug discovery and development program from the program’s inception to the final approval of the HIV drug Crixivan. Performed assay and analysis of preclinical and clinical HIV drug candidates and maintained assay records and results to comply with FDA guidelines for submission. Performed GMP fermentations of recombinant anti-malarial vaccines for Phase I clinical trials. Experienced safety officer,

conducting safety audits and implementing company safety directives.

PROFESSIONAL EXPERIENCE

MERCK RESEARCH LABORATORIES, West Point, PA 2008-2011

Research Associate, Protein Sciences Department

Development of protein purification protocols in order to supply high quality proteins to global Structural Biology and High Throughput Screening groups.

• Performed purification and characterization of tagged, untagged and secreted proteases and kinases from both prokaryotic and eukaryotic hosts.

• Collaborated with molecular biology and expression groups in order to meet critical protein delivery timelines.

• Performed experimental design, execution, data analysis and data entry into multiple databases.

• Presented on the development of purification protocols to the Protein Sciences department.

• Authored purification protocols for transfer of purification processes to outside vendors.

• Department Safety Officer for safety audits and implementation of company safety directives

• Trained laboratory personnel on protein purification techniques and instrumentation such as AKTA FPLC.

Research Biologist, Department of Structural Biology 1999-2008

Collaborated in development of protein purification protocols to support structural biology efforts. Performed purification of tagged and untagged recombinant proteins using AKTA FPLC.

• Conducted expression studies exploring various recombinant E.coli cell lines to maximize the expression of soluble proteins.

• Developed solubilization and refold methods for the untagged protein hu beta-secretase (BACE).

• Characterized proteins using SDS-PAGE, Western blots, Waters Millennium and Alliance HPLC, light scattering.

• Department Safety Officer and member of Merck Research Laboratories Executive Safety Committee.

Staff Biologist, Department of Biological Chemistry 1987-1999

Key member of HIV drug discovery and development program from the program’s inception to the final approval of the HIV drug Crixivan.

• Performed enzymatic, radioactive and HPLC assays on HIV inhibitors.

• Analysis of over 4000 HIV inhibitors synthesized by medicinal chemists resulting in development of Crixivan and inclusion in authorship of over 30 papers.

• Performed pharmacokinetic studies on preclinical and clinical HIV drug candidates.

• Maintained assay records and results to comply with FDA regulations for submission.

• Instructed laboratory personnel on the planning and execution of enzyme assays.

EARLY CAREER EXPERIENCE

SMITH KLINE RESEARCH LABORATORIES, Upper Merion, PA

Associate Scientist, Biological Process Sciences

• Performed GMP fermentations of recombinant clinical vaccines for Phase I clinical trials, assuring compliance with FDA regulations.

• Authored SOPs for new manufacturing procedures.

• Maintained fermentation documentation required for FDA audits.

COOPER BIOMEDICAL, INC., West Chester, PA

Production Chemist

• Performed purification of whole antibodies and F(ab’)2 fragments for conjugation to fluorescent dyes and enzymes in manufacture of diagnostic kits.

• Performed analytical tests such as ELISA on final protein conjugates to assure product quality.

SMITHKLINE RESEARCH LABORATORIES, Upper Merion, PA

Senior Research Technician (Contract Employee)

• Participated in studies aimed at discovery of antibacterial and antifungal agents, utilizing experience with clinical microbiological equipment and techniques.

EDUCATION

Bachelor of Science, with Honors, Biology, Chestnut Hill College, Philadelphia, PA

Elected to Delta Epsilon Sigma (National Scholastic Honor Society)

TECHNICAL TRAINING

• Biochemical purification techniques such as column chromatography (ion-exchange, affinity, SEC, HIC), dialysis and concentration of proteins, HPLC.

• AKTA FPLC automated high throughput protein purification platforms.

• Protein characterization techniques such as SDS-Page, Western blots, Agilent, Caliper, Typhoon, Thermofluor, Nanodrop, Mass Spectrometry and Bradford Protein Assays.

• Growth and recombinant protein expression with various E. coli cell lines.

• Solubilization and refold of untagged proteins.

• Enzymatic, radioactive and HPLC assays.

• Authorship of GMP manufacturing SOPs and protein purification protocols for transfer to outside vendors.

• Conducting department safety audits and implementation of company safety directives.

• Microsoft Word, Microsoft Excel and PowerPoint.

Publications available upon request.

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