Project Manager

BALA SRI RAJASA RANDHI

Ph: 361-***-**** *****,Maybird Cir

********@*****.*** Fremont,CA

Carreer objective:

To associate myself with a research organization / educational institute, where I can put my experience and knowledge to improve my skills and grow through perseverance in the areas of Molecular biology and Toxicology

EDUCATION:

2009-2011- Texas A&M University, Kingsville, TX

Masters of Science – Biology

Advisor- Dr Carlos A Garcia's

Subjects- Cell and Molecular Neurobiology, Cell Physiology,Histology ,Immunology, Toxicology,

2006-2008- University of Osmania, Hyderabad, IND

Bachelors of Science- MGC

Subjects- Microbiology, Genetics, Chemistry

Project work Accomplished: Masters Thesis

Title: "Ozone alters the concentration of Dopamine and its metabolites in the mammalian retina".

Used: HPLC-PDA(Fennigan’) & GC-M S(Harrisburg DUI Lawyer)

Handling and maintenance of different mice strains

Dissection, Sample collection and processing

Institute: Dr Carlos A Garcia's Lab, Texas A&M University Texas.

Laboratory Experience:

Biochemical Techniques and Equipments used

HPLC-PDA(Fennigan’) & GC-M S(Harrisburg DUI Lawyer)

Preparation of Standards.

Qualitative and Quantitative analysis of Catecholamine’s

Calibration, Maintenance and trouble shooting

Spectroscopy (UV and FT-IR)

Sorvall-RC6 Plus Cooling Centrifuge

Protein Purification.

Separation of Biological compounds

Gensript Toxin Sensor TM Chromogeniv LAL Endotoxin Assay kit

Enzyme Linked Immunosorbent assay(ELISA)-Mouse Elisa Kit

Brain and retinal tissue assay for protein

PAGE/SDS-PAGE (BioRad)

Protein purification (TNFalpha and TNFbete)

SHIMADZU UV-2450,UV-Vis Spectrophotometer

Estimation of total carbohydrates, total proteins

Molecular Techniques and Equipments Used

Isolation of DNA and RNA

Extraction of DNA and RNA

Purification of DNA and RNA

Cloning

Flow Cytometry

Fluorescence-activated cell sorting(FACS)

Western Blott

Protein Profiling(TNFalpha and TNFbeta)

Agarose Gel Electrophoresis/GelDoc(BIO-RAD) and Capillary Electrophoresis (CE)

Purification of DNA and RNA

PCR((9700PE-Applied Biosystems) and RT-PCR

Amplification of gene sequence

Microbiological techniques

Isolation of micro flora from different sources like environment, food and clinical samples.

Culturing and maintenance

Phenotypic and Chemotypic characterization

Preservation and reviving

Antibacterial assays

Investigation of Bio control agents

Sterilization techniques and routine lab maintenance

Animal Handling

Handling and maintenance of different mice strains

Dissection, Sample collection and processing

Handling and maintaining Drosophila fly

Work Experience

Teaching Assistant aug2009 to aug 2011

Was involved in teaching Human Anatomy and Physiology and General Biology for under graduates.

Also been involved in handling practical sessions of Anatomy and Physiology and General Biology for the same

Dr.Reddy’s labs - Jan 2006 to Dec 2008

Research Assistant

Involved in analysis of biological samples and protein purification

Involved in Cell based Screening ,Different Cell Culturing, and Staining, ELISA, Flow Cytometry(FACS)

Worked in the microbiological lab for testing the samples for different disease symptoms using CLSI methodology

Working on HPLC, GC-MS and other chromatographic methods and spectroscopy (UV and FT-IR) for the assays, wet chemistry testing including titrations and other chemical analyses

Done Western blotts and SDS-PAGE

Lab experience and GLP/GCP

Poster Presentation

Randhi, R., McIntyre, P.C., Sethi, R., and Garcia, C.A. Association for Research in Vision and Ophthalmology: June 2011. Fort Lauderdale, FL. "Ozone Decreases Dopamine Levels and Disrupts the Circadian Clock in the Rat Retina.

Computer Applications

Bioinformatics software: Basic operational knowledge on Genomics and Proteomics

MS Office: Documentations, Data entry and presentations.

Statistical analysis System: SAS Tools: SAS 9.1.3, BASE SAS, SAS/MACRO, SAS-SQL, SAS/STAT, SAS/GRAPH, SAS - ODS, SAS/ACCESS.SPSS.

SAS Procedures : PRINT, TRANSPOSE, CONTENTS, MEANS, CHART, PLOT, PRINTTO TABULATE, UNIVARIATE, SUMMARY, SORT, SQL, FREQ, FORMS, FORMAT, IMPORT, EXPORT, REPORT, GPLOT, GCHART

LANGUAGES : BASE/SAS, SQL

OPERATING SYS : MS WINDOWS, MS-DOS, UNIX, LINUX

References

Dr Carlos A.Garcia (Professor)

Texas A&M University

Kingsville,Texas

E-mail:******.*.******@*****.***

Dr Glenn Perrigo(Professor & Department Chair)

Texas A&M University

Kingsville,Texas

E-mail: *****-*******@*****.***

Dr Randy Powell(Assistant Professor)

TexasA&M University

Kingsville,Texas

E-mail: *****.******@*****.***

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