Biochemistry, Biophysics, and Data Science Leader

VICTOR SEREBROV, PhD

HOLLISTON, MA ***** 774-***-****

******.********@*****.*** https://www.linkedin.com/in/victor-serebrov S U M M A R Y

Driven and award-winning scientist with over 20 years of experience in biochemistry, biophysics, cell and molecular biology. Solid expertise in cell-based and cell-free assay development. Extensive experience in data science, statistics, and computational techniques. 10+ years of experience leading research teams in academia and industry. Additional Competencies/Specialties

• Solid track record of academic publications and novel research findings

• Strong leadership skills and reputation for ability to manage, lead, mentor, and drive team efforts towards enhanced performance

• Excellent communication skills and proven strengths with presenting research findings to key stakeholders, C-suite, and academics

• Ability to thrive in highly interdisciplinary and fast paced environment P R O F E S S I O N A L E X P E R I E N C E

Biotech Consultant March 2023 – present

Helping early-stage companies with their platform development needs in biochemistry, biophysics, pharmacology and data science space

• Working with senior level R&D clients to help grow and future-proof their company pipelines

• Providing expertise in assay development, data analytics, and research IT needs

• Helping outsource R&D activities and navigate the CRO space

• Covering RNA, oligonucleotide and small molecule therapeutics

• Assisting with developing strategic vision and timelines ENTACT BIO, INC., Watertown, MA September 2022 – February 2023 Director, Biochemistry and Biophysics

• Leadership team member at an early stage (<10 employees) biotech company

• Influenced key decisions on target selection, prioritization, validation, and screening & hit finding campaign strategies

• Established a theoretical framework, simulations, and analytical tools to design company’s proprietary heterobifunctional molecules

• Responsible for supervising and managing projects with CROs in biophysics and biochemistry space

• Contributed to building a functional laboratory from the ground up and hiring lab-based FTEs

• Established and validated a suite of cell-free molecular assays in-house VICTOR SEREBROV PAGE 2

KORRO BIO, INC., Cambridge, MA March 2021 -September 2022 Senior Principal Scientist, Biochemistry Lead

• Lead a team of RNA and protein biochemists and structural biologists focusing on generating insights to drive Korro’s proprietary oligonucleotide designs

• Built up a suite of biochemical and biophysical tools to advance the development of Korro’s oligonucleotide- mediated RNA editing platform

• Managed multiple projects with CROs, CDMOs, external collaborators and academics

• Developed bespoke screening approaches to improve design and identification of lead molecules MERCK RESEARCH LABORATORIES, Boston, MA April 2017 – March 2021 Associate Principal Scientist, Pharmacology

• Developed a high-content imaging and data analysis platform to support phenotypic screening

• Created computational pipelines to analyze large imaging datasets and provided imaging support for multiple research and clinical programs

• Designed and screened MOA-annotated and knowledge-based compound sets

• Developed imaging-based target engagement assays and uptake assays (including LNP)

• Provided expert guidance to company’s programs involving RNA therapeutics

• Created automation protocols and workflows

• Trained and managed contractors and junior scientists RNA THERAPEUTICS INSTITUTE, UMASS MEDICAL SCHOOL, Worcester, MA 2009 – 2017 Instructor, affiliated with laboratories of Melissa J. Moore, PhD, Philip Zamore, PhD, and Craig Mello, PhD

• Conceived and co-led a pioneering single molecule study on the molecular mechanisms of Argonaute proteins in RNAi and microRNA pathways

• Won the 2016 Oligonucleotide Therapeutics Society’s Paper of the Year Award for the single molecule study of Argonaute proteins

• Co-invented and patented novel technology for improved imaging, detection and affinity purification of specific RNA and DNA sequences

• Co-developed a single molecule analysis toolkit for imaging studies of complex biological processes (splicing, translation) in a cell-free system

• Developed novel technologies for imaging of biological macromolecules (ultra-bright labeling of nucleic acids; novel surface chemistry; software and tools for image processing)

• Developed methodologies for fluorescent labeling of sgRNAs and Cas9 for CRISPR/Cas9 imaging applications

• Coordinated a single molecule research group involving seven laboratories at RNA Therapeutics Institute

• Trained and mentored a group of 2-4 PhD students and PhD-level scientists

• Managed relations with instrument manufacturers and industry partners (Olympus, Zeiss, Leica, Chroma, Semrock, Mad City Labs, New England Biolabs).

YALE UNIVERSITY, New Haven, CT 2002 – 2009

Postdoctoral Scientist, Department of Molecular Biophysics and Biochemistry, Advisor: Anna Marie Pyle, PhD

• Pioneered a novel methodology to study helicase-mediated RNA unwinding with single-nucleotide resolution

• Established a detailed kinetic mechanism of RNA translocation and unwinding by a processive RNA helicase VICTOR SEREBROV PAGE 3

• Co-authored a seminal single molecule study of helicase unwinding and translocation utilizing optical tweezers

• Established molecular mechanisms of action of the NS3 helicase form Hepatitis C virus

• Contributed to understanding of enzymology of Hepatitis C virus proteins (helicase, protease, RdRp RNA polymerase) involved in the replication of the viral RNA genome. S K I L L S

• Molecular and Cell Biology: microscopy; high-content imaging; lab automation; mammalian cell culture; small molecule library screening; cell transfection; PCR; qPCR; western, northern and southern blotting; protein expression and purification; cloning; sequencing; ELISA; fluorescence-, luminescence-, radioactivity-, and cell-based assays; assay development; biochemical, enzymatic and immunoassays; homogenous assays (TRFRET, AlphaLISA).

• Biochemistry: chromatography; modifications & conjugations; enzymology; enzyme kinetics; protein expression systems (bacterial, yeast, mammalian); affinity and enzymatic protein tags and purification strategies; nucleic acid isolation, characterization, quantification and handling; RNA stability & decay; RNA structure probing; RNA-protein interactions; in vitro translation, transcription and splicing; immunoprecipitation; small-scale mRNA synthesis; ligation; site-specific labeling; preparation and fractionation of cell extracts; polysome profiling.

• Computational/Data Science: MATLAB, Python, Spotfire; machine learning; deep learning; data analytics; statistics; computer vision; high-performance computing.

• Biophysics: SPR, GCI, BLI, FP, DSF, MST, DLS, fluorescence imaging; single molecule fluorescence microscopy; TIRF microscopy; sedimentation analysis; UV-Vis spectroscopy; smFRET.

•

E D U C A T I O N A N D C R E D E N T I A L S

Doctor of Philosophy (PhD), Biochemistry, Engelhardt Institute of Molecular Biology, Russian Academy of Sciences

• Investigated RNA folding kinetics and equilibrium using classical biochemistry (RNA structure probing) and biophysics (UV and CD spectroscopy, fluorescence, hydrodynamics); discovered alternative folding pathways in small structured RNAs (tRNA); determined the role of conserved RNA modifications in RNA stability and folding. Master of Science, Chemistry and Biochemistry, Department of Chemistry, Moscow State University, Russia, cum laude equivalent

P A T E N T S

P.D. Zamore, S.M. Jolly, W. Salomon, V. Serebrov, M.J. Moore. METHODS OF USING OLIGONUCLEOTIDE GUIDED ARGONAUTE PROTEINS. Published on 10/06/2016

S E L E C T E D P U B L I C A T I O N S

1. Simov V, Altman MD, Bianchi E, DelRizzo S, DiNunzio EN, Feng G, Goldenblatt P, Ingenito R, Johnson SA, Mansueto MS, Mayhood T, Mortison JD, Serebrov V, Sondey C, Sriraman V, Tucker TJ, Walji A, Wan H, Yue Y, Stoeck A, DiMauro EF. Discovery and characterization of novel peptide inhibitors of the NRF2/MAFG/DNA ternary complex for the treatment of cancer. Eur J Med Chem. 2021 2. Braun JE, Serebrov V. 2017. Single-Molecule Analysis of Pre-mRNA Splicing with Colocalization Single-Molecule Spectroscopy (CoSMoS). In: Shi Y (eds) mRNA Processing. Methods in Molecular Biology, vol 1648. Humana Press,

New York, NY

3. Serebrov V and Moore, MJ. 2016. Single Molecule Approaches in RNA-Protein Interactions. In: RNA Processing: Disease and Genome-wide Probing. ISBN:978-3-319-29071-3 VICTOR SEREBROV PAGE 4

4. Salomon WE, Jolly SM, Moore MJ, Zamore PD*, and Serebrov V*. 2015. Single-Molecule Imaging Reveals that Argonaute Reshapes the Binding Properties of Its Nucleic Acid Guides. Cell 162, 84-95. *Co-corresponding authors Highlighted in Herzog VA and Ameres SL. 2015. Approaching the Golden Fleece a Molecule at a Time: Biophysical Insights into Argonaute-Instructed Nucleic Acid Interactions. Molecular Cell 59, 4-7 5. Shcherbakova, I, Hoskins, AA, Friedman, LJ, Serebrov V, Corrêa IR, Xu MQ, Gelles J, and Moore MJ. 2013. Alternative Spliceosome Assembly Pathways Revealed by Single-Molecule Fluorescence Microscopy. Cell Reports 5, 151-165

6. Serebrov V, Beran RKF, and Pyle AM. 2009. Establishing a mechanistic basis for the large kinetic steps of the NS3 helicase. Journal of Biological Chemistry 284, 2512-2521 7. Beran RKF, Serebrov V, and Pyle AM. 2007. The serine protease domain of hepatitis C viral NS3 activates RNA helicase activity by promoting the binding of RNA substrate. Journal of Biological Chemistry 282, 349**-***** 8. Dumont S, Cheng W, Serebrov V, Beran RK, Tinoco I, Pyle AM, and Bustamante C. 2006. RNA translocation and unwinding mechanism of HCV NS3 helicase and its coordination by ATP. Nature 439, 105-8 Highlighted in Eggleston AK. 2006. Helicase à go-go-go. Nature Structural & Molecular Biology 13, 101.; Frick DN. 2006. Step-by-step progress toward understanding the hepatitis C virus RNA helicase. Hepatology 43, 1392 – 1395; Rasnik I, Myong S, and Ha T. 2006. Unraveling helicase mechanisms one molecule at a time. Nucleic Acids Research 34, 4225 – 42231 9. Dumont S, Cheng W, Serebrov V, Tinoco I, Pyle AM, and Bustamante C. 2005. Direct single molecule observation of HCV's RNA helicase at work. Biophysical Journal 88, 350A-351A 10. Serebrov V, and Pyle AM. 2004. Periodic cycles of RNA unwinding and pausing by hepatitis C virus NS3 helicase. Nature 430, 476-480

Highlighted in Bianco PR. 2004. Hepatitis C NS3 helicase unwinds RNA in leaps and bounds. The Lancet 364, 1385 – 1387

11. Serebrov V, Clarke RJ, Gross HJ, and Kisselev LL. 2001. Mg2+-induced tRNA folding. Biochemistry 40, 6688 – 6698 12. Serebrov V, Vassilenko KS, Kholod NS, Gross HJ, and Kisselev LL. 1998. Mg2+ binding and structural stability of mature and in vitro synthesized unmodified Escherichia coli tRNAPhe. Nucleic Acids Research 26, 272*-****-**. Serebrov V, Vassilenko K, Kholod N, and Kiselev L. 1997. Mg2+ ions differently affect the physical properties of tRNAPhe and the transcript of its gene. Molecular Biology (Moscow) 31, 894-900.

Contact this candidate