Molecular Microbiology Lab Specialist with 13+ Years Experience

CURRICULUM VITAE

V DINESH

Old No: */*, New No: *, Abirami Avenue, 6th Street, Kodungaiyur, Chennai-600 118. Mobile No: 866-***-**** / 950*******. Email ID : ***************@*****.*** SUMMARY

Experienced as research associate in Molecular biology

A skilled research and lab operation professional in Diagnostic lab.

13 years+ experience in Mycobacteriology lab settings.

Ability to work under BSL-3 precautions to handle pathogenic organisms.

Use the ability to focus on details and skills to achieve high success rates in a broad range of laboratory functions.

Work efficiently with a diverse team as well as individually.

Successful in managing routine lab functions.

EDUCATIONAL QUALIFICATION

1. Periyar University, Salem, Tamilnadu.

Master of Science (M. Sc.), Major: Microbiology

2. Dr. Ambedkar Govt. Arts College, Chennai, Tamil Nadu. Bachelor of Science (B. Sc.), Major: Chemistry

3. Govt. Stanley Medical College, Chennai, Tamil Nadu. Diploma in Medical Laboratory Technology

PROFESSIONAL EXPERIENCE

1. ICMR - National Institute for Research in Tuberculosis. Chennai (ICMR-NIRT) Project Technical Officer - MARCH 2025 – PRESENT

Study Name: Estimation of Risk Associated with Zoonotic Tuberculosis in Tamil Nadu, South India (ERAzTB).

Role & Responsibilities:

Assessed zoonotic TB risk through human and cattle sample analysis

Processed human pulmonary samples and extrapulmonary samples (e.g., lymph node, tissue biopsies etc.) and cattle-derived samples (lymph node tissues, lung tissues, etc.)

Used NaOH decontamination method and performed tissue grinding, homogenization, and centrifugation

Skilled DNA extraction using CTAB method from scraped colonies on 7H11 plate, LJ media, and 7H9 liquid medium concentrate for PCR and sequencing analyses

Inoculated samples into MGIT 960, Lowenstein–Jensen medium, and Kirchner’s liquid medium and 7H11 medium.

Monitored cultures for growth, contamination, and time-to-positivity

Performed acid-fast staining (Ziehl–Neelsen) and preliminary identification of MTBC

Correlated human TB culture results with epidemiological data (cattle exposure, occupation, etc.)

Followed BSL-II/BSL-III biosafety practices and maintained quality control as per WHO, NTEP and lab SOP.

2. ICMR - National Institute for Research in Tuberculosis. Chennai (ICMR-NIRT) Project Consultant Senior Lab Technician-APRIL 2020–FEB 2025 (5 Year’s) Study Name: National TB Elimination Programme (NTEP) Role & Responsibilities:

• 10 years + experience of working in BSL III containment lab and handling of infectious microbes following bio-safety measures.

• Followed the standard operating procedures for sputum smear microscopy, Including sputum collection, smear-making, staining, and reading the smears.

• Maintain the Laboratory Register and report the results to the Medical Officer managing the patient.

• Organized work on the disposal practices of contaminated lab material as detailed in the Laboratory Manual.

• Assist STLS in the implementation of NTEP Lab Quality Assurance

• Assist in the implementation of new TB Diagnostic Tools in NTEP

• Maintenance of all logistics (reagents, project equipment, stationeries documentation of Case Report Form, Log sheets of specimen storage (raw and processed).

• Performing all tests on TB (Smear, Gene Xpert, Line Probe Assay (LPA), First Line, Second Line, NTM, Mycobacteria Growth Indicator Tube (MGIT), Lowenstein-Jensen

(LJ) and Drug susceptibility testing (DST)) and updating the results of the monthly and weekly reports of using advanced Excel spreadsheet functions as well as LIMS and NIKSHAY and promptly sending them to respective Hospitals across India.

• Performing internal quality control on daily basis.

• Making sure that the new strategy achieved according to the SOPs.

• Timely maintenance of EQA (External quality assurance) & IQA (Internal quality assurance) of laboratory methods.

• Around 80 batches of panel culture LJ (solid) and Liquid Culture (MGIT 960) were successfully sent to various National Reference Laboratories(NRLs) and Intermediate Reference Laboratories (IRLs) in India for EQA.

• Around 30 batches of panel slides were successfully sent to various IRLs and C&DST in India for EQA.

• Lab Logistics procuring and maintenance of registers as per required.

• Compiling lab procedures and preparing SOP, lab equipment maintenance log sheet and Bio-safety management protocol according to NABL norms.

• Involved in NABL activities - Preparing and verifying of laboratory related SOPS.

• Involved in renewal of NABL certificate procedures.

• Facilitating training to PG Students in TB diagnostic procedures. Accomplishments

• Maintained 100% compliance with patient confidentiality protocol.

• Maintained a 98% accuracy rating while accessioning upwards of 150 Specimen daily.

• Performed all the tests and Dispatch the results on timely manner

• Visited the States Kerala and Telangana for Onsite Evaluation under NTEP for quality assurance.

• Testing of Belgium Culture for EQA by MGIT and LPA annually to maintain QC. 3. ICMR - National Institute for Research in Tuberculosis. Chennai (ICMR-NIRT) Technical officer - DECEMBER 2014 – MARCH 2020 (5 Year 3 month) Study Name: National TB Elimination Programme (NTEP - FIND -SAMS) Role & Responsibilities:

• Followed the standard operating procedures for sputum smear microscopy, including sputum collection, smear-making, staining, and reading the smears, etc.

• Maintain the Laboratory Register and report the results to the Medical Officer managing the patient.

• Organized work on the disposal practices of contaminated lab material as detailed in the Laboratory Manual.

• Maintenance of all logistics (Drugs, reagents, kits,(FEFO) project equipment, stationeries Documentation of Case Report Form, Log sheets of specimen storage (raw and processed).

• Performing all tests on TB (Smear, GeneXpert, Line Probe Assay (LPA), FL, SL, NTM, Mycobacteria Growth Indicator Tube (MGIT), Lowenstein-Jensen

(LJ) and Drug susceptibility testing (DST)) and updating the results of the monthly and weekly reports of using advanced Excel spreadsheet functions as well as NIKSHAY and promptly sending them to Respective Hospitals across India.

• Performing internal Quality Control on Daily Basis.

• Making sure that the new strategy achieved according to the SOPs.

• Timely maintenance of EQA (External quality assurance) & IQA (Internal quality assurance) of laboratory methods.

• Lab Logistics procuring and maintenance of registers as per required.

• Compiling lab procedures and preparing SOP, lab equipment maintenance log sheet and Bio-safety management protocol according to NABL norms.

• Involved in NABL activities - Preparing and verifying of Laboratory related SOPS.

• Facilitating training to UG and PG Students in TB diagnostic procedures. Accomplishments

• Maintained 100% compliance with patient confidentiality protocol.

• Maintained a 98% accuracy rating while accessioning upwards of 150 Specimen Daily.

• Performed all the tests on time manner

• Dispatch the results in on timely manner

• Testing of Belgium Culture for EQA by MGIT and LPA annually to maintain QC.

• Nominated for onsite training of TruNAAT in Bangalore 4. RIGHT Hospitals Pvt Ltd, Chennai, Tamil Nadu.

Lab Technician - SEPTEMBER 2012 – DECEMBER 2014 (2 Year 3 month)

• Knowledge to carryout various test procedure relating in Hematology, Bio- Chemistry, Microbiology, and Blood Bank, Techniques.

• Experience in routine and culture blood test using computerized laboratory equipment.

• More than 50 + blood campus was successfully organized in and around Chennai. Accomplishments

• More than 50 + blood campus was successfully organized, Experience in Other Field in Detail:

CMS INFOSYS PVT LTD (LEADLINE SYSTEMS PVT LTD)

SUPERVISOR- AUGUST 2010 TO SEPTEMBER 2012 ( 2 Year 2 month)

• Managing six cashier’s activities.

• Entries of all transactions in online.

• Supervising all type of payment and handling Original Documents & Bonds. Publications:

1. Resistance Profiles to Second-Line Anti-Tuberculosis Drugs and Their Treatment Outcomes: A Three-Year Retrospective Analysis from South India Radha Gopalaswamy, Nandhini Palani, Dinesh Viswanathan, BershilaPreysingh, Suchithra Rajendran, Vaishnavee Vijayaraghavan, Kannadasan Thangavel, Senthil Devi Vadivel, Hannah Stanley, Kannan Thiruvengadam,Lavanya Jayabal, Kaleeswari Murugesan, Sridhar Rathinam, Asha Frederick, Gomathi Sivaramakrishnan,Chandrasekaran Padmapriyadarsini, Sivakumar Shanmugam. Medicina (Kaunas, Lithuania), 2023, 59(6), 1005.

2. Trend Of Resistance To Rifampicin And Isoniazid In Tuberculosis Patients: A Four-

Year Retrospective Analysis" Palani, N., Premkumar,M.,vaishnavee, V., Dinesh, V., Thiruvengadam, K., Lavanya, J., Sridhar, R.,Frederick, A., Sivaramakrishnan, G., Mondal, R.,. Int J Tuberc Lung Dis. 2022;26(5):446-453. 3. Improved detection of previously undetectable mycobacteria grown in liquid culture

Subramanyam, B., Sivaramakrishnan, G., Dhandapani, R.,Sangamithrai, D., Sivaraman, P., Dinesh, V., Thiruvengadam, K., Golla, R.,Nagarajan, P., & Mondal, R.

(2020). Improved detection of previously undetectable mycobacteria grown in liquid culture.

The international journal of tuberculosis and lung disease : the official journal of the International Union against Tuberculosis and Lung Disease, 24(7), 754–755. M.Sc. project Comparison of LPA and Truenat assays for the detection of Isoniazid resistance in Mycobacterium tuberculosis.

MS-office: (Word, Excel, PowerPoint, Outlook)

Handling Genolyse DNA extraction, PCR MASTER MIX, PCR, FL LPA SL LPA, NTM LPA, Molecular diagnosis, Gene Xpert, Trunaat. Sputum Microscopy, Phenotyping method Culture & DST Data Entry 40WPM.

Data Management, LIMS, NIKSHAY

Maintenance of All Logistics

• Skilled in Microsoft office

• Honors Diploma in Computer Application ( HDCA )

• Trained in C Language, C+ Language, SQL Server and Visual Basic

• LANGUAGE - English

• GRADE - Lower and Higher

Ability to multitask effectively with time management.

Ability to perform repetitive tasks with a high degree of accuracy

Comfortable working independently with minimal supervision

Active participation in Teamwork.

Ability to quickly grasp new concepts and be initiative-taking.

Ability to solve problems positively.

INTERPERSONAL SKILLS:

• Excellent analytical ability and leadership quality

• Positive attitude

• Willingness to learn and to take initiative in a challenging environment

• Hard worker.

• Capable of troubleshooting and quick in upgrading new techniques. Training Profile Other Achievements:

AREAS OF : EXPERTISE:

Computer Skills:

Typing Skills:

Key Strength:

Good Clinical Practice

Good Clinical Laboratory Practice

Honors Diploma in Computer Application

Type Writing – English

Available on Request

With this, I declare that the above mentioned particulars are true and correct to the best of my knowledge and belief.

DINESH. V

CERTIFICATION:

REFERENCES:

DECLARATION:

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