Senior Research Associate Molecular Biology & Assay Development

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Leslie Krause ***********@*****.*** • (***) 745 – 3067

LinkedIn • San Jose, CA, 95148, United States

Senior Research Associate

Experienced professional with progressive experience in molecular biology techniques, assay development, and high-throughput screening. Proficient in optimizing qPCR, ELISA, and cell-based assays to support drug discovery and biomarker research. Adept at coordinating with cross-functional teams, executing experimental protocols, and analyzing complex biological data with precision. Skilled in troubleshooting and validating novel assays in both GLP and non-GLP settings. Strong background in cell culture, nucleic acid extraction, and reagent formulation, while maintaining high scientific standards. Areas of Expertise

● PCR, qPCR, and DNA/RNA Isolation

● Immunohistochemistry

(IHC)/Immunofluorescence (IF)

● Mass Spectrometry Sample Preparation

● AAV Transduction/Cell-based Assays

● BSL-2 Laboratory Safety Protocols

● Western Blot, ELISA, and Bradford

Assay

● SDS-PAGE, UV Spectroscopy, / Gel

Electrophoresis

● Mammalian Cell Culture

● Transgenic Mouse/Rat Colony

Management

● Radioactive Ligand Binding/Beta-Arrestin

Recruitment (GPCR assays)

● Flow Cytometry/Drug Screening

● Aseptic/Sterile Technique

● Tissue Dissection, Brain Dissection, and

Cryosectioning (Cryostat)

Career Experience

Associate at Biocare Medical, Pacheco, CA May 2024 — July 2025 Conducted antibody qualification for Reagent R&D team by screening antibodies across multiple autostaining platforms, including Oncore, Oncore+, IntelliPATH, NeoPATH, and Ventana. Performed routine monthly maintenance of autostainer.

• Independently diagnosed and rectified technical issues as needed

• Supported QA/QC processes to maintain product quality and regulatory compliance.

• Coordinated closely with cross-functional teams to ensure efficient production and project execution.

• Provided rapid and accurate results with consistent turnaround efficiency in alignment with project timelines. Research Technologist III Laboratory Manager at Medical College of Wisconsin, Milwaukee, WI

August 2016 — July 2023

Directed operations of pharmacology lab focused on endocannabinoid research, specializing in CB1 and CB2 receptor pathways. Applied in-depth knowledge of GLP (Good Laboratory Practices) and regulatory standards while managing research continuity through prudent budget oversight. Designed and enforced lab protocols and SOPs to ensure accuracy, compliance, and efficiency. Oversaw procurement and inventory control of equipment, reagents, chemicals, and radioactive materials. Managed animal research operations, such as handling, breeding, genotyping, training, and healthcare of transgenic models. Executed complex biochemical and cellular assays with high precision, ensuring reproducible and reliable results.

• Improved efficiency and reduced error rates by streamlining biochemical assay workflows.

• Resolved technical and operational challenges through strategic prioritization and critical thinking. Page 2

• Supervised daily operations for research lab of 7–15 members, ensuring compliance with scientific protocols.

• Delivered high-quality data analysis and interpretation, consistently contributing to publication-ready research.

• Established operating plans, set quality standards, and resolved technical issues for optimal equipment performance. Electronics Technician at United States Navy, Norfolk, VA August 2008 — August 2012 Performed micro/miniature repairs on Combat Systems equipment, encompassing radar, satellite, communications, and weapons systems. Served as Work Center Supervisor for micro/miniature repair shop aboard U.S. Navy carrier. Executed emergency repairs, while ensuring mission-critical system readiness.

• Saved U.S. Navy $1.5+M in equipment replacement costs from 2009–2012 via effective repair and maintenance.

• Restored functionality of MK 31 Rolling Airframe Missile System by repairing critical circuit card during live combat exercise, while ensuring mission success and uninterrupted defense capability.

• Honored with Navy and Marine Corps Achievement Medal by Deputy Secretary of Defense William Lynn III for exemplary service as Medical First Responder during Operation United Response. Education

Bachelor of Art in Neuroscience & Psychology, January 2012 — January 2016 Carthage College, Kenosha, WI

Key Competencies

PCR, qPCR, and DNA/RNA Isolation Western Blot, ELISA, and Bradford Assay Radioactive Ligand Binding/Beta-Arrestin Recruitment (GPCR assays) Immunohistochemistry (IHC)/Immunofluorescence (IF) SDS-PAGE, UV Spectroscopy, / Gel Electrophoresis Flow Cytometry/Drug Screening Mass Spectrometry Sample Preparation Mammalian Cell Culture Aseptic/Sterile Technique AAV Transduction/Cell-based Assays Transgenic Mouse/Rat Colony Management Tissue Dissection, Brain Dissection, and Cryosectioning (Cryostat) BSL-2 Laboratory Safety Protocols

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