Molecular Biologist Single-Cell & NGS Expert

Rasheed Bailey

New York, NY 347-***-**** ********@******.***.*** https://www.linkedin.com/in/rbailey4 Professional Summary

Molecular biologist with expertise in single-cell and multi-omics workflows, molecular assay development, and next-generation sequencing (NGS). Experienced in designing and optimizing experiments including PCR, RNA/DNA extraction, primer design, and library preparation. Skilled in high-throughput molecular screening, quality control, and troubleshooting experimental workflows. Collaborative researcher with experience supporting cross-disciplinary teams and contributing to publications in infectious disease and molecular biology. Professional Experience

NYC Public Health Laboratory (Amentum) — New York, NY Biological Scientist Mar 2023 – Present

• Designed and performed molecular assays including primer design, DNA extraction, and real-time PCR to support pathogen detection in clinical and environmental specimens.

• Executed high-complexity testing in BSL-2 and BSL-3 laboratories, maintaining strict biosafety and quality assurance standards.

• Benchmarked and optimized molecular workflows by implementing automated data analysis scripts in R, reducing positive control QC processing time by 97%.

• Collaborated with cross-agency partners (DOHMH, NYPD, DEP, DHS) to support outbreak investigations and high-priority public health responses.

• Provided technical training in PCR, DNA/RNA extraction, and MasterMix preparation, improving team efficiency and accuracy.

Rockefeller University — Casanova Lab, Infectious Disease Department Research Specialist Jun 2021 – Nov 2022

• Performed single-cell and molecular biology assays including PCR, RNA sequencing, and primer design to study viral immunogenetics.

• Developed SOPs and optimized workflows for stem cell culture and nuclei preparation, ensuring reproducibility across projects.

• Applied NGS technologies to analyze gene expression profiles in COVID-19 patients, identifying differentially expressed genes linked to disease severity.

• Supported the development of experimental models for viral encephalitis, evaluating therapeutic targets and contributing to research publications.

• Collaborated with postdocs and students to troubleshoot experimental protocols and interpret complex sequencing data.

Mount Sinai Hospital — Advanced Heart Failure Dept. Clinical Research Assistant II Jan 2019 – Jun 2021 (Full-time); Jun 2021 – Apr 2024 (Part- time)

• Conducted molecular and cellular assays to identify therapeutic targets for pulmonary arterial hypertension (PAH).

• Designed experiments using stem cell-based disease models to evaluate novel drug candidates and optimize treatment strategies.

• Performed statistical analysis of clinical trial data and laboratory experiments, contributing to CIPHER multicenter studies.

Education

M.A. Biology – Buffalo State College, Buffalo, NY (2015 – 2020), GPA: 3.7 B.A. Biotechnology – Buffalo State College, Buffalo, NY (2010 – 2014) Skills

• Molecular Biology & Single Cell: PCR, qPCR, RNA/DNA purification, gel electrophoresis, stem cell/nuclei extraction, library preparation, SOP development

• NGS & Data Analysis: RNA-seq, NGS pipelines, R, Python, SQL, data interpretation, assay benchmarking

• Experimental Development: Primer design, workflow optimization, troubleshooting assays, high-throughput screening

• Collaboration & Communication: Cross-disciplinary teamwork, scientific writing, presentations, training and mentorship

Publications (Selected)

• Bailey, R. A., Beahm, D. L., & Skerrett, I. M. (2021). The complex and critical role of glycine 12 (G12) in beta-connexins of human skin. Int. J. Mol. Sci., 22(5), 2615.

• Katz, M. G., Hadas, Y., Bailey, R. A., et al. (2021). Efficient cardiac gene transfer… J. Thoracic & Cardiovascular Surgery.

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