Experienced Biomedical Lab Technician - Next-Gen Sequencing & Genomics
Resume:
Jennifer Meyer
**********@*****.***
Xenia, OH 45385
Cell: 937-***-****
Education Wright State University
●BS in biological science- graduation November 2009
Summary of Qualifications
●Next generation sequencing using the Illumina NextSeq, MiSeqI100+ and NovaSeq, Oxford Nanopore MinIon, and Ion Torrent Proton and PGM
●PCR experience (RT –PCR and Multiplex) with use of Quant Studio, ABI 7500, Thermocycler, JBAIDs, 3M
●Nucleic acid extraction using the King Fisher Flex, Maxwell HT, Qiacube, Q-flow, and manual extraction methods.
●Qualification and quantification of nucleic acids, DNA, and amplicon using a Synergy, Tapestation, Qubit Flurometer, Nanodrop, and Bioanalyzer
●Microbial culture (growing, isolating, and killing virus, bacteria, and/or protozoa)
●Knowledge of BSL II techniques and requirements
●Data analyses for quality assurance
●General lab skills: media preparation, serial dilutions, lab safety, pipetting, multichannel pipetting, calibration of lab instruments
Work Experience Wright-Patterson AFB September 2013 - Current
Biomedical Laboratory Technician III
Contracted to ERP ProSource 360 January 2022- current
Contracted to Siertek-January 2021-January 2022
Contracted to EIS – September 2018-January 2021
Contracted to Lukos: September 2016-September 2018
Contracted to STI-Technologies: March 2014-September 2016
Contracted to STI-Technologies: July 2013-September 2013
●Security Clearance: Position of trust.
●Next generation sequencing using the Illumina NextSeq, MiSeq and NovaSeq, Ion Torrent Proton and PGM, Oxford Nanopore MinIon
oNext gen sequencing using the Illumina NextSeq and NovaSeq for Sars CoV2 whole genome analysis.
●Assay validation and testing to produce a viable test to detect multiple different upper respiratory illnesses for overseas military.
oIncluding Artic V3 assay testing for Sars CoV2.
oExperimentation included nucleic acid extractions, PCR, E-gels, Biomek automation, and Next Gen sequencing.
●Performing automated nucleic acid extractions (King Fisher Flex, Maxwell 48, and Qiacube, or other comparable methods as determined by the PI).
●Testing nucleic acid with PCR and/or RT-PCR with laboratory instruments such as Quant Studio, ABI 7500, 3M, or thermocycler.
●Baseline contractor working on a multitude of projects.
●Assisted in the Silver Nanoparticle project.
oMicrobial culturing including but not limited to E. coli, E. faecalis, S. aureus, K. pneumonia, and A. baumannii.
oCommercially available silver wound dressings and antibiotic discs were evaluated to study their effectiveness against the previously mentioned bacteria.
oTrained Wright-Scholar interns to culture and enumerate bacteria.
●Multiplexing using pyrosequencing for the detection and typing of several bloodborne parasitic infections.
●DNA and nucleic acid concentration analysis using Nanodrop Specrophotometer, Agilent 2100 BioAnalyzer, and Qubit Flurometer.
●Cell cultures grown and analyzed from glycerol bacterial beads. Bacterial cultures include but aren’t limited to: Listeria monocytogenes, Lactobacillus plantarum, Pseudomonas aeruginosa, E. coli, Streptococcus pyogenes.
●Trained military NCO’s to assist in project specific PCR, ABI 7500, 3M Integrated Lightcycler, and Thermocyclers.
●BSL-2 laboratory techniques
YSI Inc – Xylem August 2011-July 2013
Chemical Technician
Orchid Cellmark December 2009-August 2011
Laboratory Technologist
Publications
●Daniel M Maloney, Goncalo Fernandes, Seema Jasin, Tom Williams, Sarah Namugenyi, Michael Carr, Jennifer R Meyer, Aryan Sharma, Louise Marshal, B Ethan Nunley, Hong Cie, Mateo Carvajal, Henry D Kunerth, Arif Tanmoy, Aine O’Toole, Amelia Weixler, Kelsey R. Lanter, Gabrial Gonzalez, Jaydee Sereewit, Rommel Guevara, Matt Loose, Padraic Fanning, Denisse Benitez, Juan Carlos Fernandez, Paul Cardenas, Daniel Hare, Alex Geninger, Thomas Williams, Gaia Nebbia, Anthoney C Fries, Patrick McClure, Pavitra Roychoudhury, Xiong Want, Senjuti Saha, Rebecca Dewar, Kate E Templeton, Andrew Rambaut. ARTIC RSV amplicon sequencing reveals global RSV genotype dynamics. Submitted for publication in Welcome Open Research.
●Deanna Muehleman, Bill Gruner, Vivian Hogan, Padraic Fanning, Carol Garrett, Jennifer Meyer, Kelsey Lanter, Sarah Purves, Laurie Demarcus, Jeffrey Thervil, Bismark Kwaah, Paul Sjoberg, Elizabeth Macias, Anthony Fries. Using SARS-CoV-2 Sequencing Data to Identify Reinfection Cases in MHS Beneficiaries in the DoD Global Respiratory Pathogen Surveillance Program. EID-24-0231: Submitted for publication in Emerging Infectious Diseases Journal
●Richard R. Chapleau, Monica Christian, Benjamin Connors, Christa Premo, Tim C. Chao, Juan Rodriguez, Shana Huntsberger, Jennifer Meyer, Amanda Javorina, Kenney Reynolds, David Riddle, Mark Lisanby, Clarise Starr. Early Identification of SARS-CoV-2 Emergence in the DoD via Retrospective Analysis of 2019-2020 Upper Respiratory Illness Samples. medRxiv 2021.02.18.21251368.
●Larson MA, Sayood K, Bartling AM, Meyer JR, Starr C, Baldwin J, Dempsey MP. 2020. Differentiation of Francisella tularensis subspecies and subtypes. J Clin Microbiol 58:e01495-19
●Villazana, ET., Connors, BC., Ostrow, A., Fisher, A, Goder-Reiser, B., Kovachich, Meyer, JR, Javorina, AK, and Starr, Clarise R. Elimination of Cold-Chain Transport of Gastrointestinal (GI) Specimens. June 7, 2018. American Society for Microbiology. Atlanta, GA.
●Meyer, Jennifer R., Humphrey, T., Fries, AC, Baldwin, JC, and Starr, CR. The Validation of a Next-Generation Sequencing Assay for Rapid Identification of Enteroviruses. American Society of Microbiology. San Francisco, CA. 2019.
●Jennifer R. Meyer, Kimberly D. Bear, SSgt Michael R. Buwolda, Leo P. Sorio, Lt Col (Dr.) Dara D. Regn, Maj (Dr.) Caelan M. Ford, & Richard R. Chapleau, Ph.D. Evaluating Genetic Variation as a Possible Method for Increasing Surveillance and Managing Risk of Developing Obstructive Sleep Apnea, Ramstein Aerospace Medicine Summit (RAMS) and NATO Science and Technology Organization (STO) Technical Course, 2018
●Elia Villazana, Benjamin Connors, Jennifer R. Meyer, Amanda K. Javorina, Clarise Starr. Eliminating Cold-Chain Transport of Gastrointestinal Specimens. American Society for Microbiology conference, poster presentation, 2018.
●James C. Baldwin, Jennifer R. Meyer, Elia T. Villizana, Clarise R. Starr, Amanda M. Bartling, Marilynn A. Larson, and Michael P. Dempsey. Next Generation Real-Time Quantitative PCR- and Sequence-based Assays Differentiate Francisella tularensis Subspecies and Subtypes, 2015.
Posters
William E. Gruner, Anthony C. Fries, Deanna M. Muehleman, Carol M. Garrett, Jennifer R. Meyer, Kelsey R. Lanter, Padraic M. Fanning, James F. Hanson, Peter M. Wasik, and Elizabeth A. Macias. Sequence characterization of influenza and SARS-CoV-2 in DoD beneficiaries during the 2021-2022 season: Presented at ASTMH 2023 Conference
Acknowledgments
Danielle Ali, et al. An automated, virus variant-programmable, surrogate neutralization test is qualitatively comparable to the gold standard SARS-CoV-2 cell-based neutralization assay, 2024.
Milissa U. Jones, et al. Characteristics and Long-Term Outcomes of Cardiac Complications of SARS-CoV-2 in Young Persons, 2024.
Wei Wang, et al. Antigenic cartography using hamster sera identifies SARS-CoV-2 JN.1 evasion seen in human XBB.1.5 booster sera, 2024.
Heather Goux, et al. Performance of rapid antigen tests to detect SARS-CoV-2 variant diversity and correlation with viral culture positivity: implication for diagnostic development and future public health strategies, 2024.
Andrew O’Carroll, et al. Estimating the effect of COVID-19 vaccination and infection variant on post-COVID-19 venous thrombosis or embolism risk, 2023.
Nusrat J. Epsi, et al. Precision symptom phenotyping identifies early clinical and proteomic predictors of distinct COVID-19 sequelae, 2023.
Bethany L Dearlove, et al. SARS-CoV-2 Variant Replacement Constrains Vaccine-Specific Viral Diversification, 2023.
Stephanie A. Richard, et al. Decreased self-reported physical fitness following SARS-CoV-2 infection and the impact of vaccine boosters in military service members, 2023.
Liana R. Andronescu, et al. SARS-CoV-2 infection is associated with self-reported post-acute neuropsychological symptoms, 2022.
Stephanie A. Richard, et al. Persistent COVID-19 symptoms at six months post-onset and the role of vaccination before or after SARS-CoV-2 infection: evidence from the EPICC cohort, 2022.
Nusrat J. Epsi, et al. A machine learning approach identifies distinct early-symptom cluster phenotypes which correlate with hospitalization, failure to return to activities, and prolonged COVID-19 symptoms, 2022.
Ann I Scher, et al. An Analysis of SARS-CoV-2 Vaccine Reactogenicity: Variation by Type, First or Second Dose, and History and Severity of Prior SARS-CoV-2 Infection, 2022.
Nusrat J. Epsi, et al. Understanding ‘hybrid immunity’: comparison and predictors of humoral immune responses to SARS-CoV-2 infection and COVID-19 vaccines, 2022.
Paul W. Blair, MD MHS MSPH, et al. Topological data analysis identifies distinct biomarker phenotypes during the ‘inflammatory’ phase of COVID-19, 2022.
Stephanie A. Richard, PhD, et al. COVID-19 patient reported symptoms using the validated instrument FLU-PRO Plus in a prospective cohort study: associations with infecting genotype, vaccine history, and return-to-health, 2022.
Sabrina Lusvarghi, et al. Assessment of mRNA vaccine boosters, convalescent sera, and therapeutic antibodies for neutralization against the SARS-CoV-2 Omicron variant, 2022.
Liana R. Andronescu, et al. Host and host-response correlates of COVID-19 vaccine effectiveness in U.S. Military Health System beneficiaries, 2022.
References
Dr. Clarise Starr, Wright-Patterson AFB
*********@*****.***
Dr. Anthony Fries, Wright-Patterson AFB
*******.*****.*@**.**.***
Kelsey Lanter
******.******@*****.***
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