Research Associate with 4+ years of experience in the biotech industry

Lyeth Sayegh

Waltham, MA ***** 781-***-**** *****.******@*****.***

Professional Summary

Research Associate with 4+ years of experience in advanced microscopy, immunoassays, and molecular biology across oncology, immunology, rare disease, and neurodegenerative research. Skilled in confocal and super-resolution imaging, mammalian cell culture, assay development, and statistical data analysis. Proven ability to execute independent and collaborative projects, maintain data integrity, and optimize laboratory workflows. Core Competencies

Advanced Microscopy (Confocal, Super-Resolution, Time-Lapse) • Immunofluorescence (IF) • Tissue Clearing • Whole-Mount Staining • Mammalian Cell Culture • Organoid Culture • Immunoassays • Molecular Assays • Image Analysis (ImageJ, Zen, Las X) • NTA • TCC • mBCA Protein Analysis • eTPN Lipid Analysis • Karl Fischer Titration • Water Activity Analysis • Biorepository Management • CRO Coordination • SOP Development • Statistical Analysis

(GraphPad Prism, SPSS, Excel) • Data Integrity (Benchling ELN) • Animal Handling & Dosing

(IV, SubQ, IP)

Professional Experience

Sanofi – Research Associate II, Pathology (Advanced Microscopy) Contract with Mindlance Jan 2023 – Present

● Collaborate with Immunology, Genomic Medicine, Oncology, and Rare/Neurological Disease teams to support translational research.

● Perform immunoassays, molecular assays, and automated cell-based staining on a Hamilton Liquid Handler.

● Develop and optimize protocols for culturing, sectioning, and dissociating intestinal organoids.

● Conduct mammalian cell culture (MM1.S, HeLa, Raji, NALM-6) and imaging using Zeiss LSM980, Zeiss AxioScan, and Leica SP8 platforms.

● Execute immunofluorescence, whole-mount staining, and tissue clearing protocols.

● Analyze microscopy data using Excel and GraphPad; maintain ELNs per SOP.

● Coordinate with CROs for imaging analysis and presentations.

● Serve as lab safety officer, conducting inspections and enforcing compliance. Evelo Biosciences – Analytical Research Associate

Contract with Clinlab Staffing Feb 2022 – Jan 2023

● Performed NTA, TCC, mBCA protein analysis, eTPN lipid analysis, Karl Fischer titration, and water activity assays.

● Conducted visual drug product assessments and prepared USP buffer solutions.

● Maintained data integrity via Benchling ELN and Excel; trained new staff on SOPs.

● Reviewed and approved assay data from team members. Labcorp Drug Development – In-Vivo Research Associate I Nov 2021 – Feb 2022

● Conducted biocompatibility and pharmacological studies in mice, rats, guinea pigs, dogs, pigs, and sheep.

● Administered compounds via IV, SubQ, and IP routes.

● Performed daily clinical observations and recorded data in Provantis system. McLean Hospital – Technical Research Assistant

Aug 2021 – Nov 2021

● Maintained mouse colonies, performed genotyping, and managed breeding records.

● Conducted brain extraction and histology using Leica Cryostat.

● Applied PCR, gel electrophoresis, and immunohistochemistry in neuroscience studies. University of Massachusetts Amherst – Research Assistant Kinesiology Dept. (Jan 2020 – Jan 2021): Investigated visual acuity effects on motor control for rehabilitation research.

Psychological & Brain Sciences Dept. (Sept 2018 – Dec 2019): Collected and analyzed behavioral research data on alcohol health affirmation in women. Posters/Publications

● Modelling inflammatory bowel disease using human intestinal organoids (2025)

● New Methods for Nanobody Detection in Tissues: in vivo and in vitro anti-Nanobody Antibody Strategies (2025)

● Efficient Imaging of Airway Epithelium: Unlocking Portfolio Expansion Through Whole Membrane Scanning (2025)

● Eosinophilic Crystallopathy Associated with Type 2 Inflammation in the Fra2 Transgenic Mouse Model (2024)

● Optimization of Compatible Antibody Selection for Clearing of Large Organoids and Tissue Samples (2023)

Education

B.S., Neuroscience – University of Massachusetts Amherst (2017–2021) Certifications

● Foundations of Business Strategy – University of Virginia (2024)

● Advanced Business Strategy – University of Virginia (2024)

● Private Equity & Venture Capital – University Bocconi (2024)

● Insights-Based Decision Making for Trade – Sanofi (2024)

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