Laboratory Technician Clinical Research
Resume:
Lisia Cabral
Research Study Assistant/ Laboratory Technician
Canton, MA 02021
********@*****.***
Professional Summary
• Excellent research laboratory techniques (Molecular cloning, DNA preparation, Sequencing Alignment,ELISA, qPCR, Western Blotting, Gel electrophoresis, RNA/DNA Isolation, Immunohistochemistry, Imaging)• Mammalian cell transient transfection• Experienced in animal handling, tissue sectioning and staining• Proficient in basic principles of biomedical research (formulating hypotheses, testing hypotheses,problem-solving and trouble-shooting experimental protocols)• Team-oriented; able to work independently; adept at executing multiple experiments simultaneously• Critical thinker and able to problem solve/troubleshoot with repeated success• Excellent documentation skills and maintenance of laboratory notebooks
Authorized to work in the US for any employer
Education:
Boston University - Boston, MA February 2019
Bachelor of Science degree in Biomedical and Clinical Research
Roxbury Community College - Boston, MA
Associate of Science degree in Biological Sciences
Work Experience:
Merck-Cambridge, MA May 2023 to May 2025
Research Scientist III
Perform Mass Spectrometry-based proteomics workflows for target identification and validation, working closely with proteomics scientists and colleagues in Chemistry, Pharmacology and Disease Area Biology functions to identify and quantify proteins from biologically relevant tissues, organs and cells
Develop and optimize protocols for differentiating induced pluripotent stem cells (iPSCs) into various cell types.
Work with cross-functional teams, including immunologists, chemists, and data scientists, to integrate iPSC models into broader research initiatives
Cultivate and differentiate iPSCs into relevant cell type.
Work closely with immunologists, biologists, and other scientists to integrate flow cytometry findings into broader research initiatives, contributing to the development of therapeutic strategies.
Adapting, optimizing and/or developing multi-well assays (including compound treatment of cells in a96-well format).
Experienced with liquid handlers, sub-cellular fractionation and tissue homogenization protocols
Perform chemical labelling techniques and mass spectrometry sample preparation workflows such as in-solution digestion and HPLC-based fractionation
Mammalian cell culture to support of diverse primary and cancer cell lines using different labware (from multi-layer flasks to 96-well plates)
Western Blot assays including automatic WB system such as Jess
Biogen-Boston, MA March 2022 to February 2023
Scientist II
Perform molecular cloning using different strategies including restrictive enzyme, Gibson, golden gate following the SOP
Carry out plasmid DNA preparation across different scales following the SOP
Develop and optimize protocols for differentiating induced pluripotent stem cells (iPSCs) into various cell types, including refining culture conditions and differentiation media.
Develop, implement, and maintain aseptic techniques to ensure sterility in laboratory and manufacturing environments
Maintain and manage inventories of plasmids including DNA stocks and glycerol stocks
Isolate DNA from in vitro samples and perform molecular biology assays following the SOP, including PCR, RT- qCPR, western blot and ELISA
Performed mammalian cell culture including but not limited to cell lines, primary cells, iPSCs and SH-SY5Y models
Mammalian cell transient transfection
Analyze data and document the results
Maintain organized and detailed documentation of laboratory activities following the SOP
Boston University School of Medicine-Boston, MA January 2019 to August 2021
Research Assistant
Working at the Vascular Biology Section on a research study involving the association between arterial stiffness and target organ damage in the form of microvascular damage in the kidneys, brain and eyes in mice with vascular smooth muscle deletion of transcription factor Bcl11b, and obese mice, two models of arterial stiffness.
Working on a project elaborating engineered 3D vascular tissue fabricated from aggregated mouse smooth muscle cells to mimic blood vessel.
Demonstrate a significant level of intellectual input into the design of the project.
Able to work without direct supervision; attentive to detail; and bear independent responsibility for data accuracy
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