Automation Service Engineer

Matthew R. Pocker

**** ******* *** 408-***-**** San Jose, CA. 95120 *******@*********.***

Education: Bachelor of Science, Biological Science

San Jose State University, 1998

Laboratory • Standard and Quantitative PCR • Cloning Skills: • Automated liquid handling • ELISA • HPLC • PCR assay development • DNA sequencing • Affymetrix Genechip Arrays • Cell culture • cGMP experience

Experience: Roche Sequencing Solutions, San Jose, Ca.

Automation Support Engineer, Engineering Services

March 2013 – October 2023

• Provided support for the Tecan Freedom Evo automated liquid handling robots used to perform wet chemistry tasks associated with the Harmony non-invasive prenatal test. This included IQ, OQ, PQ for new instruments as well as routine service and troubleshooting for Tecan fleet of over 40 instruments. All clinical laboratory work was performed in accordance with CLIA laboratory regulations.

• Responsible for installation, calibration, maintenance, and repair of clinical and research laboratory equipment including lab computers, thermal cyclers, centrifuges, ovens, etc. Also served as co-manager for laboratory temperature monitoring system.

• Authored preventative maintenance and calibration documentation for various lab equipment and trained lab staff on those protocols.

• Coordinated with internal lab staff and outside vendors to consistently meet equipment return to service deadlines to maximize laboratory throughput.

Complete Genomics, Inc., Mountain View, Ca.

Molecular Biologist, Reagent Manufacturing

November 2010 - August 2012

• Formulation and QC testing (pH, conductivity) of a variety of reagent and buffer solutions in support of high throughput sequencing operations.

• Formulation and functional testing of bulk master mixes used in downstream processes to generate genomic libraries. Functional testing included routine molecular biological techniques such as PCR, digestion/ligation rxns, DNA purification, agarose gel electrophoresis, and gel imaging.

• Assisted in monthly laboratory physical inventory counts and used ERP/LIMS software on a daily basis for reagent inventory and time tracking purposes.

• Trained new employees in reagent formulation, functional testing, and data analysis.

Affymetrix, Inc., Santa Clara, Ca.

Research Assistant, Reagent Manufacturing

June 2009 - April 2010

• Testing of incoming reagent raw materials utilizing HPLC for chemical identity and purity analysis.

• Bulk formulation and functional testing of kit reagents in a cGMP/GLP environment to support Affymetrix product line.

• Preparation of process deviation and NCMR reports, protocol and batch record change requests, and data reports related to reagent stability studies.

Medarex, Inc., Milpitas, Ca. Research Associate III, Molecular Immunogenetics April 2006 - June 2009

• Development of a quantitative PCR genotyping assay involving the design and validation of a comprehensive primer set for the detection of all human transgenes in transgenic mouse colony and adaptation of the assay to automated liquid handling platform (Tecan freedom Evo).

• Routine genotyping and ELISA for the detection of human transgenes and their associated antibodies for in-house transgenic mouse colony.

Affymetrix, Inc. (Formerly ParAllele Bioscience) Production Associate, Assay Services August 2004 – November 2005 (Santa Clara, Ca.)

• Generation of high quality SNP genotyping data utilizing Affymetrix high- density GeneChip DNA Microarrays involving DNA amplification, purification, fragmentation, labeling, and hybridization techniques.

• Hands-on experience with Affymetrix GeneChip Scanner 3000, Fluidics station 400/450 and associated GeneChip Operating Software (GCOS), and geneChip DNA Analysis Software (GDAS)

Deltagen, Inc., Redwood City, Ca. Research Associate II December 1999 – February 2004

Molecular Biology – Assay Development / Cloning

• Generation of DNA constructs for targeted gene knockouts in mice involving all aspects of cloning/subcloning processes.

• Assembly and analysis of mouse genomic DNA sequences using Sequencher software.

• PCR primer design and validation to facilitate identification of homologous recombination events within embryonic stem cells.

• Developed diagnostic PCR assay for the detection of Helicobacter spp. for in-house transgenic mouse colony.

Molecular Biology – Screening / Genotyping

• High throughput PCR based screening for detection of targeted gene knockouts within embryonic stem cells.

• High throughput genotyping of mouse tissues using both standard and quantitative real-time PCR methods (ABI Sequence Detection System 7900 HT).

• Utilization of basic cell culture techniques for maintenance of embryonic stem cell cultures.

• Data analysis and data entry into database.

• Training new members in the group.

Novartis Seeds, Inc., Gilroy, Ca. Research Assistant I, Molecular Markers Group March 1999 – December 1999

• DNA sequencing associated with molecular marker primer development using Perkin Elmer ABI 377.

• High throughput genotyping for the detection of disease resistance genes in various plant species involving PCR, agarose gel electrophoresis, and gel imaging.

• Genomic and plasmid DNA isolation and purification.

• Routine laboratory maintenance including ordering of all supplies and reagents, buffer and media preparation, and cleaning/autoclaving of glassware.

Computer Proficient with both PC and Mac platforms, Microsoft Word, Microsoft Applications: Excel, Microsoft Power Point, Sequencher, Primer Express, File Maker, and BLAST search tools

References: References available upon request

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