Senior Scientist

- * - Jian J. SHE CV **/**/****

CURRICULUM VITAE

FULL NAME: Jian J. SHE JOB TITLE: Senior Scientist CV DATE (day/month/year): 12-Jun-2025

OFFICE PHONE: 845-***-****

WORK EMAIL: ********@*****.***

WORK ADDRESS: B200-4112-16, Pfizer Vaccine Research, 401 N. Middletown Road CITY: Pearl River STATE: New York ZIP: 10965 COUNTRY: U.S.A. PROFESSIONAL QUALIFICATIONS AND EXPERIENCE

DEGREE/CERT INSTITUTION CITY/COUNTRY

Medical Doctor (1978-1983) Guangdong Medical University Zhanjiang, Guangdong / China M.S. Oncology and Immunology (1987-

1990)

Central South University,

XiangYa School of Medicine

Changsha, Hunan / China

Flow Cytometric Professional Training 2006: FACS Aria Cell Sorter: Flow Cytometry Course, BD BioSciences, Billerica, MA

2005: FACS LSR II Flow Cytometry Course, BD BioSciences, San Jose, CA 2002: 15th Annual Clinical Course in Flow Cytometry 2002, Northwestern University, The Feinberg School of Medicine, Chicago, IL 2001: 14th Annual Clinical Course in Flow Cytometry 2001, Dartmouth University, Dartmouth-Hitchcock Medical Center, Lebanon, NH 1999: Advanced Flow Cytometry Course, BD BioSciences, San Jose, CA 1994: Flow Cytometry Course, BD BioSciences, Mansfield, MA 1990: Flow Cytometry Course for Research, Dana-Farber Cancer Institute, Harvard Medical School, Boston, MA

McKinsey Academy Globe Leadership 2024: McKinsey Academy Courses: Globe Leadership Academy Trainings

(Asian Chapter: 6 months)

FIELD DATE

(MM/YYYY)

JOB TITLE DEPARTMENT INSTITUTION OR

COMPANY

1984-1987 Teaching Instructor Dept. of Medicine,

Cancer Research

Institute

Guangdong Medical University

1983-1985 Medical Resident Dept. of Medicine Guangdong Medical University 1986-1987 Attending Physician Dept. of Medicine,

Oncology

Guangdong Medical University

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1990-1991 Post-doctoral Fellowship Division of Tumor Immunology

Dana-Farber Cancer Institute,

Harvard Medical School

1991-1998 Post-doctoral Fellowship,

Research Associate

Division of Immunology Beth Israel-Deaconess Medical Center, Harvard Medical

School

1998-2007 Senior Research Scientist II Immunobiology, Vaccines Discovery

Research.

Wyeth Inc.

2000-2007 Manager of Flow Cytometry

Facility & Senior Research

Scientist II

Immunobiology,

Vaccines Discovery

Research.

Wyeth Inc.

2008-2009 Senior Research Scientist II Antibody Technology, Vaccine Research East

and Early Development

Wyeth Inc.

2009-2025 Senior Scientist Antibody Technology,

Bacterial Vaccines and

Technology

Pfizer Inc.

*Please list positions for last 10 years.

RELEVANT PROFESSIONAL EXPERIENCE (PLEASE SUMMARIZE): MUITIYEARS RESEARCH TECHNOLOGY EXPERIENCE:

• Research animal handling, injection, bleeding and tissue collection, and cell culture.

• Monoclonal antibody: immunization, cells fusion, cells cloning, screen, cells freeze, antibody production. Varies antibody purification and fluorochrome conjugation.

• Hybridoma antibody scale up production: expertise in media optimization and process development. Stronger experience on varies bioreactors production. Experience on WAVE systems production and with the knowledge on AMBR250 production.

• Immunofluorescent flow cytometry: FACS and FACS analyses, cell sorting and adoptive transfer of cells in animal model.

• Immunology Assays in ELISA, CBA, ELISPOT, intracellular staining and multiprobes RNase protection assay to analyze cytokines. Using intracellular staining technology to evaluate clinical vaccine trails in mouse and human study.

• Viral research: virus culture, purification, titration, infection, cytotoxicity lysis and neutralization assays for LCMV, HSV, MVA, Vaccinia virus, human papilloma virus and human immunodeficiency virus.

• Major histocompatibility complex (MHC)-peptide tetrameric complexes and MHC-peptide dimer technology. The HLA-peptide tetramer and IFN- combined assay in viral research.

• Bacterial vaccine research on MoAb production: 7v, 13v, 20v Streptococcus pneumoniae (PnC) infant and adult, Streptococcus group A and group B, Staphylococcus, Neisseria meningitidis, Clostridium difficile, Escherichia coli, Enterococcus faecalis, Klebsiella, Chlamydia psittaci, Neisseria gonorrhoeae and Lyme Borreliosis.

• MoAb production: Human herpesvirus, Haemophilus influenzae type B; Influenza A and B, Cold- adapted influenza (CAIV); Parainfluenza virus, Vesicular stomatitis virus, Respiratory syncytial virus, Cytomegalovirus, Epstein-Barr virus, and severe acute respiratory syndrome coronavirus 2 (Covid-19); and others vaccine programs: Alzheimer immunotherapy vaccine, RSV replicon prophylactic vaccine and Nicotine (NIC, NIC7 and Nic LxA) vaccine MoAb production.

• Analysis of T-lymphocyte phenotype, proliferation, and cytotoxicity lysis assays. Specializing in mucosal lymphocytes and T-lymphocytes immunodeficiency – the model of Inflammatory Bowel Diseases; IBD.

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• T-cell activation, signal transduction, Ca++ flux, kinase and tyrosine phosphorylation.

• Protein analysis: SDS PAGE, immunoaffinity purification, immunoprecipitation, Western blotting, biotinylating and iodination of cell surface proteins, and 32P and 35S biosynthetic labeling of proteins.

• Generation of T-T hybridomas.

• Immunohistochemistry: direct and indirect staining, PAP, ABC staining, immunofluorescence and horseradish peroxidase techniques.

• Thymic and extrathymic T lymphocyte development analyses.

• Bone marrow transplantation used in animal models.

• Skin and thymus graft transplantation.

• Molecular biology: RT-PCR, Southern and Northern hybridization, transfection and protein expression of gene knockout and transgenic mice, ES cell preparation.

• Strong computer and database analysis, especially in flow cytometry data acquiring, analyzing software and Excel database analyzing for vaccine clinical trials. Pharmaceutical Industry Contributions:

• Human herpesvirus preclinical study review animal model and vaccines candidates for gD2

• Cold-adapted influenza vaccines (FluMist): Established Protocols using Flowcytometry technology to evaluation of the clinical trials. Understand Memory T-cells IFN- cytotoxin expression related to the vaccine’s efficiency.

• Therapeutic Human Immunodeficiency virus vaccines: Using 25-colors flowcytometry cells sorting T- memory cells subpopulation and exciting cytokines expression to understand the vaccines efficiency.

• Leading role in produced MoAb for 7v, 13v, 20v Streptococcus pneumoniae (PnC) vaccines for infant and adult on clinical trials studies. Produced ninety-two serotypes of PnC MoAb for clinical trial UAD assays.

• Leading role in Produced MoAb for bacterial vaccines programs: o Streptococcus:

group A Polysaccharides: ClfA N2/ N3; ClfB N23; C5A, ORF554, ORF1218, ORF1358 and ORF2459

group B Polysaccharides: Ia, Ib, II, III, IV, V, VI, VII and VIII o Staphylococcus: -Toxin, CP5, CP8, LP305 and CLFA/ CLFB o Neisseria Meningitidis:

Mening polysaccharide A, B, C, Y and W135

P2086

A12, A22, B09, B22 and B44

Nimenrix vaccines

o Clostridium Difficile: Tetanus Toxin A and Toxoid B o E. coli: Diphtheria Toxin, CRM197, O1, O2, O4, O6, O15, O16, O25b, O75 and O86; FimH and PapG

o E. faecalis: DHG, Rhamno, PF2, PF3 and PF12

o Klebsiella: KLEB O1V1, O2V1, O2V2 and O9; DbpA 1 to 9 antigens o Chlamydia Pneumoniae: Lipooligosaccharide

o Neisseria gonorrhoeae: PorB and Conserved Pilin Oligomer Component o Lyme Borreliosis: OspA and OspB ST series antigens

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• Leading role in Produced MoAb for vial vaccines programs: o Influenza A and Influenza B

o Cold-adapted influenza (CAIV):

A strains

B strains

H1N1 strains

H3N2 strains

o Parainfluenza virus: HN or F glycoprotein Fraction o Respiratory syncytial virus, RSV: prefusion gB, postfusion gB; F protein 847A, 847B variants and FoldOn antigens

o Cytomegalovirus: IE1, pre-fusion gB, post-fusion gB, gB34, gB68, UL16, Trimer, Pentamer and gH-gL antigens

o Epstein-Barr virus

• MoAb programs for Nicotine Vaccines on NIC, NIC7 and Nic LxA antigens

*Please attach additional information/skills training on separate sheet. (PUBLICATION LISTING IS NOT REQUIRED) NUMBER OF PAGES ATTACHED:

BIBLIOGRAPHY:

ORIGINAL REPORTS:

Cooper, D., Pride, MW., Guo, M., Cutler, M., Mester, JC., She, J., Souza, V., York, Y., Mishkin, EM., Smith, L., Eldridge, J. and Natuk. RJ. Interleukin-12 redirects murine immune responses to soluble or aluminum phosphate adsorbed HSV-2 glycoprotein D towards Th1 and CD4+ CTL responses. Vaccine, 2004 23:236-46. Wang, B., Wang, N., Whitehurst, CE., She, J., Chen, J. and Terhorst, C. T Lymphocyte development in the absence of CD3 or CD3 J. Immunol. 1999; 162:88-94.

Wang, N., Wang, B., Salio, M., Allen, D., She, J. and Terhorst, C. Expression of a CD3 transgene in CD3 null mice does not restore CD3 and expression but efficiently rescues T cell development from a subpopulation of prothymocytes. Int. Immunol. 1998; 10:1777-1788.

She, J., Matsui, K., Terhorst, C. and Ju, S-T. Activation-induced apoptosis of mature T cells is dependent upon the level of surface TCR/CD3 but not on the presence of the CD3 ITAM. Int. Immunol. 1998; 10:1733-1740. Wang, B., Wang, N., Salio, M., Sharpe, A., Allen, D., She, J. and Terhorst, C. Essential and partially overlapping role of CD3 and CD3 for development of and T lymphocytes. J. Exp. Med. 1998; 188:1375-1380. She, J., Ruzek, M.C., Velupillai, P., Aos de, I., Sancho, J., Harn, D.A., Sancho, J., Biron, C.A. and Terhorst, C. Generation of antigen-specific cytotoxic T lymphocytes and regulation of cytokine production takes place in the absence of CD3 . Int. Immunol. 1999; 11:845-857.

Wang, B., She, J., Salio, M., Allen, D., Lonberg, N., Lacy, E., Terhorst, C. CD3- overexpressed in prothymocytes acts as an oncogene. Mol. Med. 1997; 3:72-81.

She, J., Simpson, S.J., Gupta, A., Hollander, G., Levelt, C., Liu, CP., Allen, D., van Houten, N., Wang, B., Terhorst, C. CD16 expressing CD8 + T lymphocytes in the intestinal epithelium, possible precursors of Fc R−CD8 + T cells. J. Immunol. 1997; 158:4678-4687.

Cai, X.H., Li, X.L., Li, G.C., Zhang, S.M., She, J., Sun, Q.B. The use of anticolonic cancer monoclonal antibodies for tumor diagnosis. J. of Tumor Marker Oncology, 1995; 10:(2) 53. Balk, S.P., Stevens, C., Polischuk, J.E., Probert, C., Ebert, E., She, J., Terhorst, C. Blumberg, R.S. Composition of T cell receptor/CD3 complex in human intestinal intraepithelial lymphocytes: lack of FcR chain. Int. Immunol. 1995; 7:1237-1241.

Holländer, G., Simpson, S.J., Mizuguchi, E., Nichogiannopoulou, A., She, J., Bhan, A., Burakoff, S., Wang, B., Terhorst, C. Severe Colitis resulting from aberrant thymic selection. Immunity, 1995; 3:27-38.

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Wang, B., Levelt, C., Salio, M., Zheng, D., Sancho, J., Liu, CP., Lonberg, N., Lacy, E., She, J., Huang, M., Higgins, K., Sunshine, M.J., Eichmann, K., Lacy, E., Lonberg, N. C., Terhorst, C. Overexpression of CD3-E transgenes blocks T lymphocyte development. Int. Immunol. 1995; 7:435-448. Simpson, S.J., Holländer, G., She, J., Levelt, C., Huang, M., Terhorst, C. Selection of peripheral and intestinal T lymphocytes lacking CD3- . Int. Immunol. 1995; 7:287-293. Wang, B., Biron, C.A., She, J., Higgins, K., Sunshine, M.J., Lacy, E., Lonberg, N., Terhorst, C. A block in both early T lymphocyte and natural killer cell development in transgenic mice with high-copy numbers of the human CD3E gene. Proc. Natl. Acad. Sci. USA 1994; 91:9402-9406. Liu, C.P., Ueda, R., She, J., Sancho, J., Wang, B., Weddell, G., Loring, J., Kurahara, C., Dudley, E.C., Hayday, A., Terhorst, C., Huang, M. Abnormal T cell development in CD3-zeta-/- mutant mice and identification of a novel T cell population in the intestine. EMBO J. 1993; 12:4863-75. Peter, M.E., She, J., Huber, L.A., Terhorst, C. Labeling of adenine and guanine nucleotide-binding proteins in permeabilized cells within situ periodate-oxidized nucleotides. Anal. Biochem. 1993; 210:77-85. She, J., Sun, Q. Production of monoclonal antibodies against colorectal cancer circulating antigens by in vitro immunization. J. of Monoclonal Antibody, 1993; 9:13-17. She, J., Sun, Q. Monoclonal antibodies against human colorectal cancer ---Produced by nude mice reestablishing immunofunction. 1990, M. Sc. Thesis.

She, J., Theory, and practice of medical examination (Mei R., ed.) Shanghai Medical University Press, Shanghai, p. 69- 77, 1989.

She, J., Zhao, M. Intellectual faculties structure of student synthetic evaluation. Medical Education, 1989; (9) 21. She, J. Student intelligence education exportation (Zhao M., ed.) Guangdong Medical University Press, Zhanjiang, p.15-82, 1986.

Zhao, M., She, J. Making examination effect on intelligence education. Medical Education, 1986; (1):43. ABSTRACT:

She, J., He, ZQ., Park, JJ., Jihad, T., Goymer, J., Fruhling, D., Guerrero, C., Abili, J., Gribenko, A., Swanson, K., Jones, H., and Buchkovich, N. Characterization of pre-fusion and post-fusion specific gB mAbs for HCMV vaccine development. 2024 Annual Discovery Research Meeting. Pearl River, NY. She, J., Liu, W., Goymer, J., Fruhling, D., Guerrero, C., He, ZQ., Hom, IT., Jihad, T., Abili, J., Buchkovich, N. Swanson, K. and Jones, H. Discovery of prefusion specific gB mAbs for HCMV vaccine development. 2023 Annual Discovery Research Meeting. Pearl River, NY. She, J., M. Egan, Z. Israel, M. Hagen, J.H. Eldridge and M.W. Pride. Incorporation of CD62L Marker into IFN- or Tetramer Assay Increases the Accuracy of TEM CD8+ T Cell Identification. 2004 Annual Discovery Research Meeting. Pearl River, NY.

She, J., M. Egan, Z. Israel, J.H. Eldridge and M.W. Pride. 2004. Incorporation of CD62L Marker into IFN- or Tetramer Assay Increases the Accuracy of TEM CD8+ T-cell Identification. In. Keystone Symposia: Lymphocyte Activation and Signaling. Steamboat, CO, 2004. Abstr. 324. Poster Session. Natuk, R.J., D. Cooper, M. Pride, K. Shroff, S. Bhargava, M. Guo, F. Nasar, J. She, V. Souza, K. Kourie, E. Mishkin, L. Smith, and J. Eldridge. Intranasal Administration of HSV-2 gD glycoprotein in homologous and heterologous prime/boost immunization regimes in BALB/c mice. 28th International Herpes Workshop, Madison, WI, July 26 - 31, 2003.

Pride, M.W., S. Dispoto, She, J., V. Souza, S. Cappello, D. Schoenhaut, G. Palladino, A. Georgiu, M. Connally, P. Reed, B. Forrest, J. Eldridge and E.M. Mishkin. A Randomized, Double-Blind, Placebo-Controlled Trial to Investigate Immune Responses Elicited by Liquid Formulation of Influenza Virus Vaccine, Trivalent, Types A&B, Live Cold Adapted (CAIV-T) In Healthy Adults Aged 18 To 64 Years. 2001 Annual Discovery Research Meeting at the Sagamore, Lake George, NY.

Cooper, D., M. Guo, F. Nasar, M.W. Pride, She, J., V. Souza, K. Shroff, S. Bhargava, K. Kourie, R.J.Natuk and E.M. Mishkin. Heterologous Prime/Boosting with gD/IL-12 Induces Protective Anti-HSV2 Cellular and Humoral Immunity. 2001 Annual Discovery Research Meeting at the Sagamore, Lake George, NY.

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Natuk, R.J., M.W. Pride, D. Cooper, M. Guo, She, J., V. Souza, J. Kowalski, S. Gangolli, K. Shroff, S. Bhargava, K. Kourie, D. Long, G. Kovacs, N. Vasilakis, T. Zamb, S.A. Udem, J. Eldridge, R. Ciccarelli and E.M. Mishkin. Immunogenicity and Efficacy of Venezuelan Equine Encephalitis Replicon Particle (VRP) Vectors Expressing HSV2gD or HSV2gH/gL. Poster presentation at 26th International Herpesvirus Workshop 2001, Regensburg, Germany.

Natuk, R.J., D. Cooper, M.W. Pride, K. Shroff, S. Bhargava, D. Schoenhaut, M. Sidhu, M. Guo, She, J., V. Souza, K. Kourie, J. Eldridge and E.M. Mishkin. Heterologous Prime/Boosting with gD/IL-12 Induces Protective Anti-HSV2 Cellular and Humoral Immunity. Poster presentation at 27th International Herpesvirus Workshop 2002, Cairns, Australia.

She, J., Pride, M.W., Sidhu, M., Dispoto, S., Garcia-Hand, D., Souza, V., Natuk, R. and Mishkin, E.M. Detection of Intracellular Cytokines in Activated Human and Mouse T-Cell Populations by Three-Color Flow Cytometric Analysis. Poster presentation at Keystone Symposia: Cytokines and Disease. 2000. Snowbird, UT. Long, D., J. Kowalski, R.J. Natuk, J. Strasser, Z. Israel, M. Pride, E. Schadeck, She, J., S. Dispoto, P. Piacente, S. Megati, X. DaCosta, A. Abramovitz, G. Kovacs, N. Vasilakis, D. Bernstein, E. Mishkin, J. Eldrige, S. Udem, and T. Zamb. Immunization of mice, guinea pigs, nonhuman primates with alpha virus replicons expressing the herpes simplex virus type 2 glycoprotein D. Oral presentation at the 3rd Annual Conference on Vaccine Research, Washington, DC, 2000.

Da Costa, X., R.J. Natuk, M. Pride, She, J., S. Dispoto, E. Mishkin, D. Long, A. Abramovitz and T. Zamb. Alphavirus based vectors as an HSV-2 vaccine approach. 2000 Annual Meeting of American Society of Virology, Fort Collins, CO.

Natuk, R.J., M.W. Pride, Z. Israel, J. Kowalski, D. Long, A. Abramovitz, E. Schadeck, She, J., S. Dispoto, S. Gangolli, V. Souza, P. Piacente, S. Megati, G. Kovacs, N. Nasilakis, J. Eldridge, S Udem, T. Zamb and E.M. Mishkin. Immunogenicity of alphavirus replicon particles expressing HSV2gD in mice and non-human primates. Poster presentation at 25th International Herpesvirus Workshop, 2000. Portland, OR. She, J., V. Roopchand, V. Souza, M.W. Pride, M. Sidhu, R.J. Natuk and E.M. Mishkin. Optimization of Culture conditions to evaluate viral Specific IFN- +CD8+ T-Lymphocyte Responses. 2000 Annual Discovery Research Meeting at the Sagamore, Lake George, NY.

Pride, M.W., S. Dispoto, She, J., G. Palladino, V. Souza, S. Burke and Nursing Staff, B. Forrest, J. Eldridge and E.M. Mishkin. Investigation of Both Humoral and Cellular Immune Responses Elicited by an Influenza Virus Vaccine

(FluShield ) in Healthy Adults. 2000 Annual Discovery Research Meeting at the Sagamore, Lake George, NY. Schoenhaut, D., E. Rojas, F. Immermann, R. Natuk, She, J., K. Kruta and E. Mishkin. Gene Expression Profiling of THE IL-12 Adjuvanted Immune Response to Glycoprotein D of Herpes Simplex Virus. 2000 Annual Discovery Research Meeting at the Sagamore, Lake George, NY. Natuk, R.J., She, J., M.W. Pride, D. Chen, and E.M. Mishkin. Augmentation of humoral immune responses to herpes simplex virus type 2 gD subunit vaccine using synthetic CPG oligonucleotide sequences. Poster presentation at 24th International Herpesvirus Workshop, 1999. MIT Cambridge, MA. Long, D., She, J., and R.J. Natuk. A herpes simplex virus (HSV) microneutralization assay using the commercial ELVISTM

cell line. Poster presentation at 24th International Herpesvirus Workshop, 1999. MIT Cambridge, MA. Sidhu, M., M.W. Pride, R.J. Natuk, She, J., D. Chen and E.M. Mishkin. CpG motifs within the HSV-2 gD DNA vaccine vector are effective immune adjuvants. 1999 Annual Discovery Research Meeting at the Sagamore, Lake George, NY.

Natuk, R.J., She, J., M.W. Pride, D. Chen, and E. M. Mishkin. Augmentation of Humoral Immune Responses to Herpes Simplex Virus Type 2 gD Subunit Vaccine Using Synthetic CpG Oligonucleotide Sequences. 1999 Annual Discovery Research Meeting at the Sagamore, Lake George, NY. Natuk, R.J., M.W. Pride, She, J., D. Chen and E.M. Mishkin. Enhanced Immunogenicity of HSV-2 gD DNA Vaccine by Boosting with HSV2 gD and IL12 proteins. 1999 Annual Discovery Research Meeting at the Sagamore, Lake George, NY.

Pride, M.W., R.J. Natuk, M. Sidhu, She, J., D. Chen and E.M. Mishkin. Co-Administration of Cytokine DNA Plasmids

(IL12, IL18, or GM-CSF) with HSV-2 gD DNA Plasmid Vaccines Enhances both Humoral and Cellular Responses Directed Against gD2. 1999 Annual Discovery Research Meeting at the Sagamore, Lake George, NY.

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She, J., M.W. Pride, R.J. Natuk, and E.M. Mishkin. Detection of Intracellular Cytokines in Activated Human and Mouse T-Cell Populations by Three-Color Flow Cytometric Analysis. 1999 Annual Discovery Research Meeting at the Sagamore, Lake George, NY.

Long, D., R.J. Natuk, D.Tummolo, M.W. Pride, She, J., D. Chen, J. Kowalski, S. Gangolli, T. Zamb and E.M. Mishkin. Characterization of the Structural and Immunogical Properties of HSV-2 gD. 1999 Annual Discovery Research Meeting at the Sagamore, Lake George, NY.

The Herpes Team (in alphabetical order): A. Abramovitz, X. DaCosta, S. Gangolli, J. Kowalski, D. Long, E.M. Mishkin, R.J. Natuk, R. Pogulis, M.W. Pride, She, J., D. Tummolo and T. Zamb. A new approach to a vaccine for Herpesvirus. 1999 Annual Discovery Research Meeting at the Sagamore, Lake George, NY. She, J., Ruzek, M.C., Velupillai, P., Aos de, I., Sancho, J., Harn, D.A., Biron, C.A., Terhorst, C. Signal transduction trough CD3 is not required for the generation of antigen specific cytotoxic T cells and cytokine production in peripheral T lymphocytes. Poster presentation at Keystone Symposia: T-Lymphocyte Activation, Differentiation and Death. 1998. Keystone, CO.

She, J., Simpson, S.J., Gupta, A., Holländer, G., Levelt, C., Liu, CP., Allen, D., Houten, N., Wang, B., Terhorst, C. CD16 expressing CD8 + T lymphocytes in the intestinal epithelium, possible precursors of Fc R−CD8 + T cells. Poster presentation at Keystone Symposia, 1997. Santa Fee, NM. Wang, N., Wang, B., Salio, M., Allen, D., Gupta, A., She, J., Terhorst, C. The effect of CD3 and CD3-

deficiencies on T lymphocyte function and development. Poster presentation at AAAAI/AAI/CIS Joint Meeting, 1997; San Francisco, CA. J. of Allergy and Clinical Immunology. Jan. Suppl. 1997. Wang B., She, J., Allen D., Salio M., Lonberg N., Lacy E., Terhorst C. A hit-and-run model of lymphomagenesis caused by a non-enzymatic CD3- protein. Poster presentation at Gordon Research Conference on Cancer, August 1996, Newport, RI.

She, J., Simpson, S.J., Holländer, G., Levelt, C., Liu, CP., Wang, B., Terhorst, C. Fc R expressing CD8 + T lymphocytes in the intestinal epithelium is a precursor of Fc R−CD8 + T cells. Poster presentation at FASEB Summer Research Conference - Fc Receptors: Genes, Signalling and Function, July 1996. Snowmass Village, CO.

Wang B., Zheng D., She, J., and Terhorst C. Fas antigen plays a role in thymocyte selection by lowering the threshold level of CD3- induced apoptotic signal. Oral and poster presentation at Annual meeting of American Association of Immunologists (AAI), June 1996, New Orleans, LA. She, J., Holländer, G., Simpson, S.J., Liu, CP., van Houten, N., Howard, M., Huang, M., Wang, B., Terhorst, C. TCR intestinal intraepithelial lymphocytes which utilize the Fc RI chain instead of CD3− express the FcR II/III on their surface and are of thymic origin. Oral presentation at The Ninth International Congress of Immunology, 1995; San Francisco, CA.

Simpson, S.J., Holländer, G., She, J., Mizuguchi, E., Nichogiannopoulou, A., Gutierrez-Ramos, J., Bhan, A., Burakoff, S., Wang, B., Terhorst, C. The role of T lymphocytes in murine IBD. Oral presentation at The Ninth International Congress of Immunology, 1995; San Francisco, CA.

She, J., Simpson, S.J., Holländer, G., Wang, B., Terhorst, C. Precursor-Product relationship among CD8 + intestinal intra-epithelial T lymphocytes. Oral and poster presentation at the Eight International Congress of Mucosal Immunology, 1995; San Diego, CA.

REVIEWS:

Terhorst, C., Wang, B., Simpson, S.J., She, J., Huang, M., Wileman, T., Holländer, G., Levelt, C., Exley, M. Plasticity of the TCR/CD3 Complex. Owen, M. ed 1995, 347-380. Terhorst, C., Exley, M., Franco, R., Hall, C., Kang, J., Mueller, B., Sancho, J., She, J., Wileman, T. Coupling of T-cell activation with T-cell receptor assembly. Year Immunol. 1993;7;1-24. She, J. Production of monoclonal antibodies by in vitro immunization. 1990, M. Sc. Thesis. She, J. Suppresser T lymphocyte and autoimmune diseases. Acta of Guangdong Medical College. 1981; 1-6.

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STANDART OPERATION PROTOCOLS:

LM_Methods for Detect Granzymes and Perforin in FACS Calibur. 2005. LM_Methods for Tetramer and IFN- double staining in Flow Cytometry analysis. 2005. LM_Detection of Enterotoxins by FACS analysis of TCR V expression. 2006. LM_Methods for Detect Human PBMC Cytokines Expression in Clinical Trial. 2006. LM_Methods for Review of Human PBMC Cytolysis Potential in Clinical Trial. 2006. SOP-001: Prepare Myeloma cells for hybridoma fusion SOP-002: Generation of Mouse Hybridomas, 2023

SOP-003: Hybridoma Cloning by Limited Dilution, 2023 SOP-004: Hybridomas Cloning by cell-sorting protocol SOP-005: Thawing of hybridomas, 2021

SOP-006: Cryopreservation of mouse hybridomas, 2025 SOP-010: Generation of Analytical Working Cell Bank (AWCB) for Hybridoma cell line, 2021 SOP-011: General Screen ELISA Assay Protocol

SOP-012: End-Point Titration ELISA for Mouse Titers Protocol SOP-013: Competition ELISA Assays Protocol

SOP-014: Isotyping mouse mAbs using Iso-strips

SOP-015: Western blot: Mini-blotter Assay Protocol SOP-016: General Octet Assay for mouse IgG concentration, 2022. SOP-017: General Octet Assay for human IgG concentration, 2022. SOP-018: General Competition Binding Assay by Octet Analysis SOP-019: Determine mAbs Antibody Affinity by Octet analysis SOP-020: Indirect Enzyme Linked Immunosorbent Assay SOP-021: Large Scale TCS Purification Using HiTRAP™ Protein G HP SOP-022: Purification of Ascites Using HiTRAP™ Protein G HP SOP-023: Small Scale Antibody Purification from Hybridoma Cell Culture Using Protein G Magnetic Beads, 2024 SOP-024: Western Blot for Purity Examination Protocol, 2025 SOP-025: RNA lysate preparation for sequency from hybridoma cells, 1999 SOP-029: Hybridoma Large TCS Production by Flasks, 2022 SOP-030: Sequencing Mouse Monoclonal Antibodies, 2024 SOP-031 Sequencing Rat Monoclonal Antibodies, 2024 SOP-032: Antigen-Microsphere Conjugation for Luminex Multiplex Assays, 2025 SOP-033: Hybridoma Higher Concertation Antibody Production with Hollow Fiber Bioreactor. 2025

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