Senior Scientist

Siddhartha S. Saha, Ph.D.

Waltham, MA

781-***-****; ********@*****.***

www.linkedin.com/in/siddhartha-saha-a95088157

Highly motivated, great attention to details, enthusiasm for science, organization and leadership skills. Ability to make complex decisions, taking initiatives to begin new ventures, solving issues. Data assessment skills with extensive achievements and knowledge in immunology, autoimmunity, infectious diseases, genetics, oncology, T cells. Skills in developing and performing in vitro immunology, cell-based screening assays (ELISA, RNA isolation, luminex, NGS etc.), including cell culture, 2D, 3D, organoids, co-culture, organ-chip technology, multiplex assays with T cells, myeloid cells and in vivo models. Skills in identification of biomarkers, high-throughput mass-spectrometry and multi-OMICs datasets analysis, gene editing by CRISPR CAS9 system, lenti-vectors and AAVs. Interpreting results, efficient reporting of scientific data, generation of SOPs; excellent learning ability, critical thinking, record keeping, troubleshooting, multitasking, documentation, communication skills. Worked effectively and efficiently with scientists, managed CROs. Mentored scientists, graduates and undergraduates.

SKILL SETS

●Cell Culture/Assays: Culture of the tumor and normal or transformed cell lines, primary cultured cells, 3D cell culture, in vitro cell based assay development including luciferase reporter and cell viability assays.

●Molecular biology: Western blot (iBlot, ChemiDoc XRS and LI-COR Odyssey multiplex fluorescent imagers), Co-immunoprecipitation (Co-IP), protein expression, transient transfection, DNA sequencing, Site-directed mutagenesis, PCR, RNA isolation, multiplex qRT-PCR (QuantStudio™ 3), RNA interference (siRNA), next-generation sequencing (NGS), gene editing by lenti-vectors, AAVs, CRISPR CAS9, CHIP assay, molecular cloning, designing primers, vectors using gBlocks (pcDNA3.1, PCMV6, lenti etc.), HEK293 cell line engineering.

●Immunology: Immunization, enrichment of immune cells, immunoelectrophoresis, immunodiffusion methods, ELISA, Luminex, flow cytometry (BD LSR2, Attune NxT, BD FACSCalibur etc.), BD FACSAria sorter, live-cell imaging, ICC using confocal microscopy (Nikon A1, Leica SP5, Leica SP8 confocal).

●In vivo experiments: Ease in working with mice and rats; oral gavaging; intraperitoneal, subcutaneous and intravenous injections; blood collection from hepatic portal vein, retro-orbital plexus, tail-vein, transgenic disease models using cre-lox mice, preparation of tissues, primary cells and bone marrow derived stem cells.

●Biotech: Drug discovery, Target validation, project management, experiment design, PK/PD, regulatory documents, timeline, LC-MS, gastroenterology, neuroscience, data-dtiven decision making, bioinformatics tools, proteomics, in vivo/ex vivo assays. Well proficient in using various data software like Excel, Prism, SnapGene, Sigma Plot, Origin 8, Photoshop, ImageJ, FlowJo for data assessment.

PROFESSIONAL AND SCIENTIFIC EXPERIENCE

Snorkel AI, Inc.

Scientific Contributor / Trainer: Immunology & Biology (Contract / Task-Based) 10/2024 - 04/2025

●Generate scientific questions with a graduate / undergraduate level difficulty.

●Review questions generated by others, justify the correct answer in-terms of scientific reasoning and contributor provided options.

AllianThera Boston Inc. (ATB), Natick, MA

Senior Scientist- Immunology 09/2021 - 11/2022

●Setting up the immunology lab in different ways e.g., procuring research instruments, reagents, generating SOPs for the preliminary immuno-screening studies of drug molecules.

●Managed the lab, developed and optimized SOPs for immunology assays (Th17/Treg/Th1 differentiation, Pan-CD3+ T cell activation assays etc.), enhancing lab efficiency and productivity, executed complex experiments, analyzed and effectively reported data from in-vivo efficacy studies to the scientific team.

●Worked efficiently with CROs, cross-functional teams, leaderships and vendors to carry out the pre-clinical testing of potent drug candidates.

●Efficacy studies of drug molecules in in-vitro systems and in-vivo setups of ulcerative colitis (UC), cross-reactivity using PBMCs, metabolic stability and API release studies of prodrugs, EC50 and IC50 determination to fulfill the company's objective of discovering potent small drug molecule inhibitors / agonists of Ahr to treat autoimmune diseases (UC and Crohn’s disease) and cancer.

Immunity and Immunotherapies, Seattle Children’s Research Institute, Seattle, WA, US

Fellow Ph.D., with Adrian Piliponsky, Ph.D. 12/2018 - 2021

●Evaluation of the key mast cell surface protein repertoire by glycoprotein enrichment and mass-spec analysis identified CD98 with the potential to control mast cell functions and proliferation, mast cell cancer or mastocytosis. CRISPR CAS9 gene editing was performed to delete CD98hc in mouse primary BMCMCs.

●Executed infection experiments to elucidate the mast cell immune responses during COVID19 onset; T effector cells (Th17/Th22 axis), ILCs differentiation in ulcerative colitis (UC), mast cell derived FXIIIA in GBS infection, basophil derived TNF-a in sepsis.

Dept. of Molecular and Cellular Biochemistry, Indiana University, Bloomington, IN, US

Postdoctoral Fellow, with Cheng C. Kao, Ph.D. 3/2014-11/2018

●Investigated the mechanism of IL-36 receptor (IL-36R) signaling and trafficking through the endosomal vesicles in the pathogenesis of inflammatory diseases such as psoriasis and UC.

●Endocytosis of IL-36R upon IL-36 binding, the role of adapter protein Tollip in IL-36R signaling and trafficking to lysosomes, secretion of IL-36 cytokines, biomarkers associated with fibrosis.

Dept. of Pathology and Cell Biology, Immunology-Oncology Research Axis, Maisonneuve-Rosemont Hospital, University of Montreal (Montreal, QC, Canada) Postdoctoral researcher (Award, Immunology), with Janos G. Filep, MD 2013 - 2014

●Studied the role of endogenous anti-inflammatory lipid mediators and human formylated mitochondrial peptide on neutrophil apoptosis and resolution of inflammation during sepsis through FPR2 receptor.

Dept. of Physiology, Dept. of Applied Chemistry, University of Calcutta, Kolkata, India,

Ph.D. Student, with Mahua Ghosh, Ph.D. 2008-2013

●Investigated the biochemical and anti-inflammatory properties of naturally occurring bioactive octadecatrienoic fatty acids e.g., conjugated linolenic acid (CLnA) isomers (α-eleosteric acid and punicic acid), assessed their synergistic role in combating oxidative stress, inflammation, modifications in erythrocyte membrane morphology. Oils containing CLnA isomers showed anti-inflammatory role and inhibited the proinflammatory nuclear factor-κB (NF-κB), plasma TNF IL-6 and IL-1β levels.

Immunology section, Dept. of Physiology, University of Calcutta, Kolkata, India,

M.Sc., with Biswadev Bishayi, Ph.D. 2005-2007

●Investigated the mechanism of viability of a pathogenic bacterium e.g., Staphylococcus aureus inside the macrophages. Bacterial catalase (CAT) and superoxide dismutase (SOD) combat reactive oxygen species enabling S. aureus to persist within macrophages, inducing local inflammation, causing greater induction of serum TNF- and IL-6.

EDUCATION

2008 – 2013 PhD Dept. of Physiology, Dept. of Applied Chemistry, University of Calcutta (Kolkata, India)

2005 – 2007 M.Sc. Dept. of Physiology, University of Calcutta (Kolkata, India), 1st class and secured 3rd position

2002 – 2005 B.Sc. Dept. of Physiology, Tripura University (Tripura, India), 1st class and secured 2nd position

TEACHING EXPERIENCE

●Served as a Biochemistry lecturer for Master’s and B.Tech. curriculum at GNIT, Kolkata during the Ph.D. tenure

●Mentoring – Undergraduates, Graduate students

FELLOWSHIP/AWARDS

2008 - 2010 University with potential for excellence (UPE) Junior Research Fellowship (University of Calcutta, Kolkata, India)

2010 - 2013 Senior Research Fellowship (Indian Council of Medical Research, New Delhi, India)

2011 Indian Council of Medical Research Foreign Travel award

2013 Post-Doctoral Award Fellowship of the Maisonneuve-Rosemont Hospital Foundation and Student Affairs Committee

2013 Dr. Santinath Ghosh Memorial research award (2013) for the best Social / Industrial implication

SELECTED PEER-REVIEWED ARTICLES

Piliponsky A. M., Saha S. S. 2022, Mast cell derived FXIIIA contributes to sexual dimorphic defense against Group B Streptococcal Infections, JCI

Saha S. S. 2021, Mast cell surfaceome characterization reveals CD98hc is critical for optimal cell function. JACI

Saha, S. S. 2018, E3 ubiquitin ligase RNF125 activates interleukin-36 receptor signaling and contributes to its turnover. J Innate Immun

Yi, G., Saha S. S. 2016, Structural and functional attributes of the interleukin-36 receptor. JBC

Saha, S. S. 2015, Signal transduction and intracellular trafficking by the interleukin 36 receptor. JBC

Saha, S. S.#, 2013, Protective effect of conjugated linolenic acid isomers against arsenite induced renal toxicity in rat model, Nutrition

Saha, S. S.#, 2012, Synergistic effect of conjugated linolenic acid isomers against induced oxidative stress, inflammation and erythrocyte membrane disintegrity in rat model, BBA

Saha S. S. 2011, Antioxidant and anti-inflammatory effect of conjugated linolenic acid isomers against streptozotocin-induced diabetes. Brit J Nutr

Saha S. S. 2011, Eur J Nutr. 10.1007/s00394-011-0233-0.

Saha S. S. 2011, Chem-Biol Inter. 10.1016/j.cbi.2011.02.030.

Das, D., Saha S. S. 2008, Intracellular survival of Staphylococcus aureus: correlating production of catalase and superoxide dismutase with levels of inflammatory cytokines. Inflamm Research.

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