Quality Control Microbiology Laboratory

Home: **** ******** ****, ********, ** *****. ****.*********@*****.***;

Tel. #: 404-***-**** (Mobile) ****.******@****************.*** 1

Resume

Name: Prem L. Sharma

Education:

**** *.**. ******l Laboratory Technology (Hons), All-India Institute of Medical Sciences, New Delhi, India.

1979 M.Sc. Microbiology, Haryana Agricultural University, Hissar, India. Thesis: Studies on the enterotoxin production by Bacillus cereus. Haryana Agricultural University, Hissar, India 1979.

1985 Ph.D. Medical Microbiology, Lady Hardinge Medical College, Delhi University, India. Thesis: Epidemiology of R-plasmids encountered in Salmonella species of human origin. Delhi University, Delhi, 1984.

Professional Certifications:

Since May 2007 American Medical Technologist-MT (AMT) Summary of Skills: Leadership/Supervisory/IRB/Biosafety/Grant Management 08/1999- 08/2015 1. Supervised and trained junior scientists in BSL-2 practices. 2. Served on Atlanta VA Medical Center Safety Research Sub-committee (2000-2013). 3. Served as a Chairperson for Safety Research Committee (2009-2011) (10-15 members). 4. Served as interim Biosafety Officer for 6 months.

5. Served on Institutional IRB for five years (2007-2012) 6. Interacted with institutional and federal agencies to manage the federal grants to and collaborated with internal and external faculty to accomplish the goals of the projects. 7. Supervised and trained BS/MS/MD/Ph.D. level scientists in research methodology. 8. Expertise in biological assays related to virology, bacteriology, recombinant DNA technology (cloning), protein expression and cell biology (transfection), viral cell culture assays at BSL-2+ level.

9. Formal skills in teaching and training

10.Extensive clinical Microbiology Laboratory experience with reading culture plates, and identifying pathogenic organisms, and QA/QC

11. Developed SOPs for various research projects according to OSHA/CDC requirements Title and affiliations and job-related Skills:

01/2023- Microbiologist, Department of Infection Control and Prevention, Grady Memorial Hospital, Atlanta, Georgia.

Monitoring and advising the Safety of patients due to potential infections through contact before admission or nosocomial. Monitoring the presence of various microorganisms in patient’s room by microbiological methods. Surveillance through tracking of patient’s movement in and out of the hospital. Interacting with environmental services of Grady Memorial Hospital to monitor the potential pathogens in patient environment before and after cleaning various surfaces. Providing microbiological assistance during epidemics.

Jan. 2020- current Medical Technologist (Microbiology), Quest Diagnostics, Tucker, Atlanta A. Expertise on the identification of Streptococcus groups by reading Throat culture plates. Identification of various groups of Streptococcus using STREPTEX Latex grouping system and performing antibiotic susceptibility using VITEK 2 system. B. Identification of Group B streptococcus (GBS) from Rectovaginal swabs and performing Strep B sensitivity to antibiotics using VITEK 2 system. Home: 4416 Dunhaven Road, Dunwoody, GA 30338. ****.*********@*****.***; Tel. #: 404-***-**** (Mobile) ****.******@****************.*** 2

C. Performing identification of pathogenic bacteria from lower respiratory tract by reading plates inoculated with sputum and other respiratory-related clinical specimens and using MALDI and VITEK to report final ID and sensitivity to antibiotics. D. Advanced knowledge of Quality Control of Diagnostic Bacteriology Laboratory: performing QC for reagents and media received in laboratory; maintaining a series of aerobic and anaerobic QC organisms. Performing MALDI-ID for new lot Matrix and slides. Sept. 2013-current Medical Technologist PRN (Microbiology), Piedmont Hospital, Atlanta (Microbiology Laboratory)

Processing various body fluids for microbiological analysis at Piedmont hospital: performing weekly and daily QCs on Microscan for the ID/antibiotic susceptibility of Gram positive, Gram negative and Rapid ID panels; performing new lot QCs for various panels received in the laboratory; plating various clinical specimens and reading Gram stains; processing positive blood cultures and communicating with Nurse/Dr.; reading STAT CSF Gram stain and India ink and wet preps for Trichomonas; performing PCR on GeneXpert Infinity and BioFire Diagnostics 07/2006-08/2007 Staff Medical Technologist (Generalist), Archbold Memorial Hospital, Thomasville, GA Worked as a full time Staff Medical Technologist in evening shift; processed specimens for Microbiology, hematology, clinical chemistry, blood bank and immunology/serology. Sept. 1999-August 2015 Research Microbiologist/Assistant Professor, Atlanta Veterans Affairs Medical Center Studies on HIV-1 drug resistance and viral fitness. Other Skills: Supervisory/Biosafety/Grant Management 08/1999-08/2015 Developed SOPs for various research projects based on OSHA/CDC requirements; Supervised and trained junior scientists in BSL-2+ practices; served on Atlanta VA Medical Center Safety Research Sub-committee (SRS) since 2001; served as a Chairperson for Biosafety Committee (two years)

(10-15 members committee); served on Institutional IRB for three years; interacted with institutional and federal agencies to manage the federal grants to accomplish the goals of specific project; supervised and trained BS/MS/MD/Ph.D. level scientists in research methodology. Publications 40 in peer-reviewed scientific journals Scientific Conferences More than 20 oral/poster presentations Invited Lectures More than 10 Job-related and other power-point presentation

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