Quality Control Molecular Biology

Vijayasshekar Manukonda

Cary, NC ***** USA

+1-319-***-**** *************@*****.*** https://www.linkedin.com/in/vijayasshekar-manukonda-8b904732/ - Summary

Professional Laboratory Technologist with extensive experience executing high complexity testing and quality control. Demonstrates proficiency in instrument operation, reagent preparation, and meticulous record-keeping, ensuring adherence to strict regulatory standards. Brings a robust background in molecular biology and biochemical techniques, driving process improvements and supporting assay validations.

Education

JNT University, Hyderabad, India

PhD, Biotechnology Hyderabad, Telangana,India

Acharya Nagarjuna University, Guntoor, AP, India

MSc, Biotechnology Guntur, Andhra Pradesh, India

Experience

Syngenta Crop Lab Nov 2023 - Jun 2024

Research Assistant-3 RTP, NC

Detected effector proteins secreted by pathogens using precise laboratory testing procedures, ensuring detailed records were maintained to enhance plant defense response research.

• Mapped gene product specificities by leveraging advanced biochemical and structural techniques while ensuring quality control documentation for reproducibility.

• Engineered and infiltrated gene expression cassettes in soybean and tobacco plants, following standard laboratory procedures including meticulous record keeping.

• Conducted preliminary studies to identify significant correlations between pathogen effectors and resistant genes, adhering to established quality assurance protocols.

University of North Carolina Sep 2022 - Apr 2023

Research Associate Chapel Hill, NC

Investigated T cell-mediated autoimmune responses and the AIM2 inflammasome interactome using rigorous molecular and biochemical techniques, ensuring compliance with laboratory quality control procedures and maintaining meticulous records.

• Engineered retroviral fusion constructs in mammalian expression vectors and generated viral particles for mapping AIM2 binding partners, operating under established laboratory standard operating procedures and detailed documentation practices.

• Published research findings in 2023, demonstrating effective data interpretation and adherence to quality assurance standards. Oklahoma State University Nov 2021 - Aug 2022

Research Associate Tulsa, OK

I researched ADAMTS metaloproteases involved in cardiovascular and inflammatory diseases, focusing on their roles in hemostasis and extracellular matrix remodeling. My work specifically examined the allosteric regulation and substrate specificities of ADAMTS13 and ADAMTS7, highlighting their implications in thrombotic thrombocytopenic purpura (ITP), a serious blood clotting disorder.

• Purified Recombinant Proteins: Successfully purified multiple recombinant substrates of ADAMTS-13 and ADAMTS-7 using ad- vanced affinity and gel-filtration chromatography techniques.

• Established Stable Cell Lines: Developed stable cell lines for ADAMTS-13 and ADAMTS-7, in mammalian cell lines and facilitated continuous protein expression for in vitro binding assays to identify potential substrates. Skills

• Molecular Biology: Molecular biology, Recombinant cloning in E.Coli, Recombinant cloning in Insect cells, Plasmid extraction, DNA/RNA extractions, PCR, RTPCR, qPCR, MS office, Recombinant cloning in Mammalian cells

• Biochemical: Biochemical, FPLC, Protein purification, Affinity Chromatography, Ion-Exchange chromatography, Gel Filtration chromatography, In-vitro assays, SDS PAGE, Western Blot, Protein-Protein Interaction Studies (e.g., Co-immuno-ppt, Proximity labeling etc), Surface Plasmon Resonance(SPR, Graph pad prism, bioinformatics tools, pull down assays, ELISA, Spectrophotometry

• Cell Culture: Cell culture, Microbiology, Microscopy (Light, Fluorescence), Virus particle generation (Lentivirus/Adenovirus or Retro virus), Cell Transfections and transductions, cGMP and FDA Regulations Compliance, transient transfections, Insect and Mammalian cells, stable cell lines generation

• Certificates: ASCP(MB) valid up to 2028

• Laboratory Operations: Quality control procedures Published Research Findings

• Qi Ke, Ashley Nicole Greenawalt, Veera Manukonda, Xingqi Ji, Roland Michael Tisch. The regulation of self-tolerance and the role of inflammasome molecules. Frontiers in Immunology (Review article, Accepted manuscript) March,2023.

• Joshua Muià, J. Cameron Barton, BS, Kathryn L. Smith, BS, Karen Vanhoorelbeke, Veera V Manukonda, Anna Platt, BS4 and Frida Miranda.. Regulation of VWF Cleavage Is Mediated By Coupling ADAMTS13 D- and C- Domain Binding Sites. Poster Abstract

(Blood: https://doi.org/10.1182/blood-202*-******) Nov,2022.

• Sekhar Reddy*, Jianfang Liu*, Anurag Misra*, Mark White, Gang Ren. Structural plasticity of neuraxin 1 a: implications for its role as synaptic organizer. Journal of Molecular Biology, Aug,2018.

• Zhuoyang Lu, M.V.V.V. Sekhar Reddy, Jianfang Liu, Ana Kalichava, Jiankang Liu, Lei Zhang, Fang Chen, Yun Wang, Luis Marcelo F Holthauzen, Mark A White, Suchithra Seshadrinathan, Xiaoying Zhong, Gang Ren, and Gabby Rudenko. Molecular Architecture of Contactin-associated Protein-like 2 (CNTNAP2) and its Interaction with Contactin 2. J Biol Mol Chem. Sep,2016.

• Zhuoyang Lu, Yu Wang,Fang Chen, Huimin Tong,M.V.V.V. Sekhar Reddy,Lin Luo Suchithra Seshadrinathan,Lei Zhang,Luis Marcelo F. Holthauzen,Ann Marie Craig, Gang Ren, Gabby Rudenko. Calsytenin-3: molecular architecture and interaction with neuraxin 1alpha. J Biol Chem. 2014 Dec 12; 289(50):34530-42.

• M.V.V.V.Sekhar reddy. G.Chakshusmathi, Sunil K. Panigrahi, H.Subramanya, M.Lakshminarsu. Diphenylether derivative as selective inhibitor of PTP1B over TCPIP identified through virtual screening study. Mini reviews in medicinal chemistry Volume 13, Number 11,2013,1602-1606.

• M.V.V.V.Sekhar reddy, Chakshusmathi Gadiyaram, Sunil Kumar Panigrahi, Narasimha Rao Krishnamurthi, Subramanya Hosahalli, Arun Chandrasekharappa, Deepankar Manna, Sangamesh E. Badiger, Pramod K.Dubey,Lakshmi Narsu Mangamoori. X-ray crystal structure of PTP1B in complex with a new PTP1B inhibitor. Protein & Peptide Letters journal Volume 21, Number 1, January 2014, pp. 90-93(4).

• Arun P. Chandrasekharappa, Sangamesh E. Badiger, Pramod K. Dubey, Sunil K. Panigrahi, Sekhar Reddy V.V.V. Manukonda. Design and synthesis of 2-substituted benzoxazoles as novel PTP1B inhibitors. Medicinal Chemistry Letters, Volume 23, Issue 9, 1 May 2013, Pages 2579-2584.

• Arun P. Chandrasekharappa, Sangamesh E. Badiger, Pramod K. Dubey, Sunil K. Panigrahi, Sekhar Reddy V.V.N. Manukonda. Design and synthesis of novel 2-substituted benzothioole compounds as PTP1 inhibitors. Letters in Drug Design & Discovery Volume 11, Issue 4, 1 May 2013, Pages 444 - 453.

• M.V.V.V.Sekhar Reddy, G.Chakshusmathi, M. Lakshmi Narasu. Small molecule inhibitors of PTP1B and TCPIP. Int.J.Pharm.Faphy- topharmacol.Res. 2012, 1(5): 287-291.

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