Information Technology Molecular Biology

Andrew Stan

646-***-**** **********@****.***

Objective

A dedicated professional with a strong foundation in Information Technology and a background in biology, seeking to merge my expertise in both fields. Eager to leverage my IT skills and experience in genetic research to contribute to innovative solutions in biotecnology, bioinformatics, computational biology, and data analysis. Passionate about applying technological advancements to biological challenges, with a long-term goal of advancing research and development in the life sciences. Summary : Biology Lab

Experienced biology researcher with a strong focus on genetic manipulation techniques in mice, specializing in zygote and nucleus transfer to study genetic inheritance and cellular reprogramming. Led advanced experiments in somatic cell nuclear transfer (SCNT) to explore cloning potential and developmental biology.

Experience includes studying metabolic pathways and the molecular mechanisms underlying yeast respiration in Saccharomyces cerevisiae. Specialized in exploring the roles of mitochondrial and cytoplasmic tRNA synthetases in cellular energy production. Proficient in molecular biology methods, including PCR, gel electrophoresis, Southern and Western blotting, to assess genetic integration and expression following nuclear transfer. Molecular Biology Techniques: Proficient in PCR (Polymerase Chain Reaction), Southern Blotting, Northern Blotting, and Western Blotting techniques for DNA, RNA, and protein analysis. DNA Sequencing: Skilled in Sanger sequencing and other manual sequencing methods for identifying nucleotide sequences in genes and genomes. Cloning and Recombinant DNA Technology: Experience with bacterial transformation, plasmid construction, the use of restriction enzymes for cloning, gene expression, and genetic modification. Gel Electrophoresis: Expertise in performing agarose and polyacrylamide gel electrophoresis for DNA, RNA, and protein separation and analysis.

Genetic Mapping and Analysis: Proficient in genetic linkage mapping and performing experiments to identify loci associated with traits in model organisms (e.g., Drosophila, C. elegans, or mice). Cell Culture and Transfection: Experienced in maintaining cell lines, transfecting cells with plasmids, and studying gene expression in vitro.

Fluorescence Microscopy: Competent in using fluorescence microscopy techniques to study gene expression, protein localization, and cellular structures. Radioactive Tracing: Knowledge of using radioactive isotopes (e.g., P-32, S-35) for nucleic acid labeling, hybridization, and autoradiography.

Chromatography and Spectrophotometry: Familiar with the use of various chromatography techniques (e.g., affinity, ion exchange) and UV-Vis spectrophotometry for protein and nucleic acid quantification.

Statistical Analysis: Ability to perform data analysis using software like SPSS and Microsoft Excel, as well as interpret experimental results in the context of genetics and molecular biology. Experience : Biology Lab

Columbia University, New York, NY

Research Assistant

09/ 1 9 9 3 – 09/ 1 9 9 6

Conducted experiments to analyze the metabolic pathways and the role of mitochondrial and cytoplasmic tRNA synthetases in yeast respiration of Saccharomyces cerevisiae. Isolated and characterized proteins involved in the respiratory cycle of Saccharomyces cerevisiae, utilizing techniques such as Western blotting and mass spectrometry for protein identification and analysis.

Assisted in the design and execution of molecular biology techniques, including PCR, cloning, and transformation, to create yeast strains with modified tRNA synthetases for functional studies. Maintained up-to-date knowledge on advancements in the field of biology by attending professional development workshops and conferences.

Moscow State University, Moscow, Russia

Graduate Research i n GENETICS

09/ 1 9 9 2 – 0 6 / 1 9 9 3

Conducted advanced research on genetic manipulation in mice, specifically focusing on zygote and nucleus transfer techniques to study genetic inheritance and cellular reprogramming. Led experiments involving somatic cell nuclear transfer (SCNT) in mouse zygotes to investigate the potential for cloning and developmental biology.

Utilized advanced molecular biology techniques, including PCR, gel electrophoresis, and Southern blotting, to assess genetic integration and expression after nuclear transfer. Designed and optimized protocols for mouse embryo culture, fertilization, and in vitro manipulation, achieving successful embryo development and implantation. Analyzed data from genetic assays to identify the impact of nuclear transfer on gene expression patterns and cellular differentiation in early-stage development. Collaborated with a multidisciplinary team of geneticists, embryologists, and biotechnologists to explore applications of nucleus transfer in transgenic animal models and regenerative medicine. Publications

Available at: https://www.researchgate.net/scientific-contributions/Andrey-Shtanko-33366644 Characterization of the COQ5 gene from Saccharomyces cerevisiae: Evidence for a C-methyltransferase in ubiquinone biosynthesis. May 1997.

Cloning and identification of HEM14, the Yeast Gene for mitochondrial protoporphyrinogen oxidase. November 1996.

SCO1 and SCO2 Act as High Copy Suppressors of a Mitochondrial Copper Recruitment Defect in Saccharomyces cerevisiae. September 1996.

Characterization of COX17, a Yeast Gene Involved in Copper Metabolism and Assembly of Cytochrome Oxidase. July 1996.

Cloning and identification of HEM14, the Yeast Gene for mitochondrial protoporphyrinogen oxidase. January 1996.

Mitochondrial and Cytoplasmic Isoleucyl-, Glutamyl-and Arginyl-tRNA Synthetases of Yeast are Encoded by Separate Genes. July 1995.

Summary : Information Technology

Results-driven and detail-oriented software developer with extensive experience in designing, developing, and deploying Java-based applications. Proficient in multiple programming languages including Java 8, Perl, Python, and JavaScript, with expertise in Java/J2EE technologies such as JSP, Servlets, EJB, and JMS. Strong background in XML technologies (XML, JAXP - SAX, DOM) and web services (SOAP, WSDL, AXIS), enabling the development of efficient and scalable web solutions. Well-versed in modern development methodologies such as Agile, Scrum, and Test Driven Development (TDD). Experienced in working with both SOAP and RESTful Web Services, and leveraging front-end technologies like JQuery. Skilled in database management with hands-on experience using Oracle 9/10g, MS SQL Server, and JDBC for seamless data integration.

Languages : Java 8, J2EE : JSP, Servlets, EJB, JDBC, JMS ; Perl, Python, JavaScript XML Technologies : XML, JAXP (SAX, DOM)

Web Services : SOAP, REST

ORM : Hibernate, Spring, Spring Boot, Spring Batch, Struts Deployment : Jenkins

Version Control : GIT, SVN

Scripting : UNIX shell – Bourne Shell (sh), Bourne Again Shell (bash), Korn Shell (ksh) Application/Web Servers : WebSphere, WebLogic, Jboss, Tomcat DataBases : Oracle 9/10g, MS SQL Server, MySQL, DB2 Methodologies : Agile, Scrum, Test Driven Development(TDD) Experience : Information Technology

Developed and maintained high-performance Java-based applications, focusing on Java 8 features such as lambda expressions and streams for improved code readability and efficiency. Utilized Spring Framework (including Spring Boot, Spring Batch, and Spring MVC) to design and implement scalable, modular, and testable applications with a strong focus on dependency injection, AOP, and transaction management.

Implemented Hibernate ORM to streamline database interactions, optimizing data access with lazy and eager loading strategies, and leveraging Spring Data for seamless repository management. Developed multi-threaded applications using Java concurrency APIs, such as ExecutorService and synchronized blocks, ensuring optimal performance and scalability in high-load environments. Designed and integrated SOAP and RESTful Web Services to enable efficient communication between disparate systems and external APIs, adhering to best practices for security and scalability. Employed JMS for asynchronous messaging in distributed applications, improving overall system efficiency and responsiveness.

Applied Test Driven Development (TDD) within an Agile/Scrum environment to ensure high-quality code, utilizing JUnit and Mockito for unit and integration testing. Used Jenkins for continuous integration and automated deployments, streamlining the build, test, and deployment cycles to increase productivity and reduce downtime. Managed source code and version control through GIT and SVN, ensuring efficient collaboration and maintaining code quality across the development team. Wrote UNIX shell scripts (Bash, Korn Shell) to automate repetitive tasks, streamline deployments, and enhance system administration workflows.

Worked with multiple relational databases such as Oracle 9/10g, MS SQL Server, MySQL, and DB2, optimizing SQL queries and ensuring seamless data flow across various systems. Deployed applications on enterprise-level application servers like WebSphere, WebLogic, JBoss, and Tomcat, ensuring high availability, load balancing, and performance optimization in production environments.

Led and participated in code reviews, ensuring adherence to coding standards, and collaborated closely with product owners and other stakeholders to deliver high-quality software solutions. Employment History : Information Technology

F reelance Software Engineer 03/ 2 0 2 0 – C u r r e n t P l anet Payment, Garden City, NY; Software Engineer 01/ 2 0 1 9 – 12/ 2 0 1 9 F reddie Mac, McLean, VA; Software Engineer 10/ 2016 – 12/ 2 0 1 8 GE Healthcare, Park R i dge, NJ; Software Engineer 11/ 2013 – 03/ 2 0 1 6 FXDD, New York, NY; Senior Developer 12/ 2007 – 03/ 2 0 1 3 UBS, Weehawken, NJ; Senior Developer 12/ 2005 – 11/ 2007 NYU, New York, NY; Developer 05/ 2005 – 11/ 2005

Delta Funding, Woodbury, NY; Developer 12/ 2007 – 03/ 2 0 1 3 AMP Agency, New York, NY; Developer 06/ 2004 – 01/ 2005 Accenture: JPMorganChase, New York, NY; Developer 10/ 2002 – 06/ 2004 BottleRocket / ACTV, New York, NY; Developer 08/ 2000 – 09/ 2002 Colson Services, New York, NY; Developer 08/ 1 9 9 9 – 05/ 2000 Bank Hapoalim, New York, NY; Developer 09/ 1 9 9 7 – 08/ 1 9 9 9 Education

Lehman College, Bronx, NY

BA i n B i ology

1 9 9 5

Moscow State University, Moscow, Russia

MS i n B i ology

1 9 9 3

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