Postdoctoral Associate

* * * * *

Shams G. Shams, PhD

Address: ** **** ******* ****,

New Brunswick, NJ 08901

E-mail: ******@*****.*******.***

Phone. 732-***-****

Nationality: USA Citizen

A. EDUCATION

College of Pharmacy, Cairo University, Cairo, Egypt 10/2020 PhD in Pharmaceutical Sciences, Biochemistry

Thesis Title: Amelioration of copper oxide nanoparticles-induced oxidative stress by dehydroepiandrosterone either alone or in combination with Quercetin on rat liver: in vitro and in vivo study

College of Pharmacy, Zagazig University, Zagazig, Egypt 08/2014 Master of Pharmaceutical Sciences, Biochemistry.

Thesis Title: New insight on the role of melatonin or dehydrepiandrosterone (DHEA) hormones in management of partially hepatectomized and/or cirrhotic rats’ liver Academy of Scientific Research and Technology, Cairo TOT (Training of Trainers) Certificate: Teaching

09/2011

College of Pharmacy, Zagazig University, Zagazig, Egypt B.Sc. Pharmacy

Final year grade: Excellent with percent of 92.5.

General Graduation: Excellent with honor cumulative (Top of the class). 07/2009

College of Pharmacy, 6th of October University, Egypt. Clinical Pharmacy Diploma

07/2008

Gained clinical pharmacy experience.

B. Appointments

a) Research Experience:

Postdoctoral Associate: Department of Chemical Biology, Ernest Mario School of Pharmacy, Rutgers University, New Brunswick, NJ, USA. 11/01/2023- Present

Modulating one-carbon metabolism with diet and targeted inhibitors to treat triple negative breast cancer using quantitative metabolism tools, such as metabolomics and stable isotope tracing; to discover how homeostasis of one-carbon metabolism is achieved to transform nutrients into the chemical material for controlling chromatin structure through methylation of histone proteins and DNA.

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Trained 5 undergraduate students, one technician. Postdoctoral Fellow: Department of Medicine, Robert Wood Johnson Medical Hospital, Rutgers University, New Brunswick, NJ, USA. 08/30/2022 – 10/31/2023 Researched G protein-coupled receptors (GPCR) regulation of metabolic disorders: breast/liver cancer, obesity-induced fatty liver disease using molecular biology techniques.

Investigated the therapeutic potential of kisspeptins (KPs) in addressing liver fibrosis associated with non-alcoholic fatty liver disease (NAFLD) and non-alcoholic steatohepatitis

(NASH). By confirming KISS1R (that is the receptor for kisspeptin) expression in hepatic stellate cells (HSCs) and demonstrating KISS1 and KISS1R expression in human hepatic stellate cells (LX-2) cells. These investigations employed tissue culture approaches and animal models.

Employed cell culture models, genetically engineered mice.

Trained 3 undergraduate students, 2 technicians, and one post doctorate fellow. Postdoctoral Fellow: Cancer Biology, Biomedical Sciences Department, Virginia College of Osteopathic Medicine (VCOM)-Monroe Campus, Monroe, LA, USA.

(My grant) 01/15/2022 – 08/30/2022

Identified novel targets against oncogenic signaling pathways associated with metastatic, castration-resistant prostate cancer (mCRPC).

Investigated the role of exosomes in altering signaling pathways within tumor cells and their microenvironment to promote prostate cancer progression and metastasis. These investigations employed tissue culture approaches, animal models (Xenograft and transgenic), and human specimens.

Executed protocols, ensured safety, analyzed data, and supervised lab tasks.

Trained 2 undergraduate student and three post doctorate fellows. Researcher (Lecturer): Therapeutical Chemistry Department, National Research Center (NRC), Cairo, Egypt. 12/01/2020 – 01/14/2022

Investigated the effect of Quercetin and Omega-3 against ethanol-induced gastric ulcer in rats.

Investigated the efficacy of Egyptian Clementine oil identified by GC/MS analysis on Alzheimer’s disease–induced rats.

Trained 80 undergraduate students.

Provided teaching lessons to undergraduate students on biochemistry.

Prepared models for various biological systems.

Provided training to doctoral and master students.

Participated in various departmental activities. 3 P a g e

Research Associate: Therapeutical Chemistry Department, National Research Center (NRC), Cairo, Egypt. 03/2014 – 11/2020

Tracked inventory of lab supplies needed for experiments and worked with lab manager for purchasing.

Maintained and run the lab.

Routinely presented posters at various national and international scientific meetings. Research Assistant (Teaching Assistant): Therapeutical Chemistry Department, National Research Center (NRC), Cairo, Egypt. 01/2010 – 02/2014

Trained to adhere to Good Laboratory Practice (GLP) and Good Documentation Practice

(GDP).

Trained on how to conduct research.

Trained on surgical procedures on rats.

Provided training to undergraduate students on molecular biology research techniques.

Provided teaching lessons to undergraduate students on pharmaceutics and industrial technology.

b) Teaching Experience

Research-focused teachable unit in cancer, delivered to undergraduates at 3 minority serving institutions affiliated with the Rutgers-IRACDA postdoctoral training program, which provides salary support for my research and teaching: William Paterson Univ (New Jersey), New Jersey City Univ., and Medgar Evers College-CUNY (Brooklyn) 2025

Biochemistry, Pharmacology and Pharmaceutics courses, delivered to undergraduate and graduate students, Egyptian Pharmacist Syndicate 2015-2021

Licensed TOT (Training of Trainers). 2019

Taught and trained students on lab techniques, NRC. 2014-2021 Biochemistry and Lab Techniques course, Suez Canal University. 2019 CV Writing and Research Writing course, Suez Canal University. 2019 Industrial Techniques course, EPSF, Zagazig University and Suez Canal University. 2019 Lab Techniques course, Egyptian Pharmacist Syndicate, Cairo 2018-2021 C. OTHER WORK EXPERIENCE

Clinical Scientist Internship Rotation at Genmab, New Jersey, USA. 04/25-06/25 Medical Writing Internship Rotation at Genmab, New Jersey, USA. 01/25-04/25 Community Pharmacist, Cairo, Egypt.

- Asl Pharmacy 2014-2015

- El-Ahlya Pharmacy 2013-2014

- Fadl Pharmacy 2012-2013

Internship at Delta Pharm Co., Cairo, Egypt. 2010-2011 Internship on lab techniques in El-Borg Lab, Cairo, Egypt. 2008-2009 4 P a g e

Clinical Pharmacist, El-Ahrar Hospital, Zagazig, Egypt. 2009-2012 D. SKILLS

Molecular biology and biochemistry techniques

a. Cell culture: human cancer cell lines such as HepG-2, Lx2, CWR-R1ca, MCF-7, MDA-MB- 231, BT549, PC3 and 22RV1.

b. Microscopy: fluorescence and light microscopy

c. Quantitative real-time PCR analysis

d. ELISA

e. Western blot (WB) analysis

f. Flow cytometry (apoptosis and cell cycle analysis) g. Liquid Chromatography Mass Spectrum (LCMS)

Rodent research techniques

Mouse handling (restraint and weighing) • anesthetizing • mouse breeding. Intraperitoneal and subcutaneous injections • tail vein sampling • surgical operations; partial hepatectomy surgery on rats, osmotic pump implantation • behavioral tests (open field test, T-maze test).

Computer Skills

• Statistical Software (SPSS), Endnote citation program, GraphPad prism (data visualization and analysis) • ImageJ (quantifying fluorescent intensity for WB) •Microsoft Excel (data entry, analysis, and organization) • Microsoft Word (scientific writing) • Microsoft PowerPoint (data organization for presentations)

E. OTHER SKILLS

a. Metabolomics Data Analysis

b. Proficient in operating and maintaining complex lab equipment. c. Student research mentoring

d. Public lecturing

e. Lab report completion procedures

f. Proposal writing

g. Lab supervision

h. Laboratory safety protocols

F. CERTIFICATES

Practical Aspects of Clinical Trial Design and Conduct, Rutgers University, NJ, USA 2025

SciPhD - The Business of Science: Applying the Scientific Method to Succeed in Industry, 5 P a g e

Rutgers University, NJ, USA 2024

CITI Program course, Basic biosafety training, Laboratory Safety, ULM, USA 2022 Western blotting technique training, NRC, Cairo 2021 Comet and ELISA techniques training, NRC, Cairo 2018 Bioinformatic course, NRC, Cairo 2017

Metabolomics workshop, NRC, Cairo. 2017

G. FELLOWSHIPS AND AWARDS

Postdoctoral Fellowship from NIH IRACDA K12 GM093854, Drs. Martha Soto, Gary Brewer, and Detlev Boison (PI/co-PIs, respectively) to Rutgers University. The K12 provides mentored teaching and research training for postdoctoral fellows. 09/2024-present Award of appreciation for Encouraging and Engaging Underrepresented Youth in Research

,RUYES program, funded by NIH/ NCI program 09/2025 From Science to Pharma MSL Fellowship with Rutgers University. 07/2024 Postdoctoral Fellowship to study in the USA, Egyptian Mission Association. 01/2022- 08/2022 Teaching Assistant preparation courses grant, Misr-ELkheir foundation. 05/2014 H. PUBLICATIONS

a) Published (Note: in Egypt, follow Canadian guidelines where we can be first or last author) 1) Shams, S. G., Dawud, D., Michalak, K., Makhlouf, M. M., Moustafa, A., Jazwinski, S. M., Kang, L., Zerfaoui, M., El Sayed, K. A., & Abd Elmageed, Z. Y. (2024). Blockade of neutral sphingomyelinase 2 exerts antitumor effect on metastatic castration resistant prostate cancer cells and promotes tumor regression when combined with Enzalutamide. American journal of cancer research, 14(12), 5697–5716.

2) Prasad, K. *; Bhattacharya, D. *; Shams, S. G. E.*; Izarraras, K.; Hart, T.; Mayfield, B.; Blaszczyk, M. B.; Zhou, Z.; Pajvani, U. B.; Friedman, S. L.; Bhattacharya. M. (2024). Kisspeptin Alleviates Human Hepatic Fibrogenesis by Inhibiting TGFb Signaling in Hepatic Stellate. Cells, Preprints 2024, 202-***-****. https://doi.org/10.20944/preprints202409.1111.v1 3) Shams GE Shams, Ron-Joseph Ocampo, Sanna Rahman, Maysoon M Makhlouf, Jihad Ali, Magdy M Elnashar, Hassan L Ebrahim, Zakaria Y Abd Elmageed: Decoding the secrets of small extracellular vesicle communications: exploring the inhibition of vesicle-associated pathways and interception strategies for cancer treatment. American Journal of Cancer Research 2024;14(5):1957-1980

4) Shams, S. G. E., & Eissa, R. G. (2022). Amelioration of ethanol-induced gastric ulcer in rats by quercetin: implication of Nrf2/HO1 and HMGB1/TLR4/NF-κB pathways. Heliyon, 8(10), e11159. https://doi.org/10.1016/j.heliyon. 2022.e11159 6 P a g e

5) Elmaidomy, A. H., Abdelmohsen, U. R., Alsenani, F., Aly, H. F., Shams, S. G. E., Younis, E. A., ... & El Amir, D. (2022). The anti-Alzheimer potential of Tamarindus indica: an in vivo investigation supported by in vitro and in silico approaches. RSC Advances, 12(19), 11769- 11785.

6) Aly, H. F., Younis, E. A., Gaafar, A. A., Shams, S. G. E., Ahmed, K. A., Hashish, H. M. A., & Salama, Z. A. (2022). The efficacy of Egyptian Clementine oil was identified by GC/MS analysis on Alzheimer’s disease–induced rats. Egypt. J. Chem, 65(3), 465-477. 7) Abdelazeim, S. A., Shehata, N. I., Aly, H. F., Shams, S. G. E. (2020). Amelioration of oxidative stress-mediated apoptosis in copper oxide nanoparticles-induced liver injury in rats by potent antioxidants. Scientific Reports, 10(1), 1-14.

8) Foda, D. S. and Shams, S. G. E. (2020). A trial for improving thyroid gland dysfunction in rats by using a marine organism extract. Brazilian Journal of Biology, 81, 592-600. 9) Aly, H. F., Mohamad, H. E., Soliman, A. M., Shams, S. G. E. (2014). Role of melatonin in management of partially hepatectomized and/or propagated-cirrhotic livers of rats. Int J Pharm Sci Rev Res, 26, 11-31.

b. Pending Publications:

1) Rana G. Eissa, Shams GE Shams: An in vivo investigation of Omega-3 effect against ethanol- induced gastric ulcer in rats. Submitted

2) Maha Zaki, Hanan Farouk Ali, Shams GE Shams: Exploring novel compounds with therapeutic efficacy for management of brain disorders and impaired insulin signaling in Alzheimer's disease. In preparation

3) Maha Zaki, Hanan Farouk Ali, Shams GE Shams: Optimization of the production of bioactive compounds from agricultural wastes of the farm and food factories in therapeutic applications and food industries. In preparation

I.Recent Posters:

1) Maya Saez, Shams GE Shams, Mathew Mcbride. Targeting Methionine Metabolism in Triple-Negative Breast Cancer: Ag270-Induced S-adenosyl Methionine Depletion Impairs Metastasis. (2024) RUYES cohort 4, CINJ, Rutgers University, NJ. 2) Kelly Herrara, Shams GE Shams, Mathew Mcbride. The Effect of MAT2A Inhibitor on the Metastasis of Triple Negative Breast Cancer Cells (2024) RISE program, Rutgers University, NJ.

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3) Shams GE Shams, Stephania Gouzman, Moshmi Bhattacharya. Inhibitory effects of Kisspeptin on the activation of hepatic stellate cells. (2023) Rutgers University Postdoctoral Research Symposium, NJ.

4) Tia Hart, Shams Shams, Moshmi Bhattacharya. Evaluation of Kisspeptin on the Migration and Growth of Hepatic Stellate Cells and Hepatocellular Carcinoma Cells. (2023) RUYES Cohort 3, Poster Symposium, Rutgers University.

5) Gad S, Shams GS, Zerfaoui M, Abd Elmageed ZY. Small extracellular vesicle-derived microRNAs as a diagnostic and therapeutic tool in melanoma resistance.(2022) 38th Southern Biomedical Engineering Conference. Hilton, New Orleans Airport, LA. 6) Hassan D, Shams GS, Taha H, Abd Elmageed, ZY. Novel combination of protein SUMOylation inhibitor and Enzalutamide inhibits the aggressive phenotypes of prostate cancer cells. (2022) American Osteopathic Association (AOA). Osteopathic Medical Conference & Exposition (OMED).

*Complete list of presentations and posters upon request.

Contact this candidate