Research Scientist Molecular Biology

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Curriculum vitae

Dr. Sivakumar Allur Subramanian, M.Phil., Ph.D.

Research Scientist

Department of Orthopaedics & Rehabilitation

Carver College of Medicine, University of Iowa, United State E.mail: ************@*****.***

*********-****************@*****.***

LinkedIn ID: Sivakumar Subramanian_LinkedIn

Education

Doctor of Philosophy, Biochemistry, Annamalai University, India 2007-2012 Master of Philosophy, Biochemistry, Annamalai University, India 2005-2007 Master of Science, Biochemistry, Bharathidasan University, India 2003-2005 Bachelor of Science, Biochemistry, University of madras, India 1998-2002 Research Work Experience

Assistant Research Scientist (2024 Dec-At present) Department of Orthopedics & Rehabilitation,

Carver College of Medicine

The University of Iowa, United State.

Senior Researcher-(2021-2024)

Department of Orthopedic Surgery,

Hallym University Dongtan Sacred Heart Hospital, Korea. Research Area: Translational Regenerative Medicine & Developing Biomaterials. Research Professor- (2015-2020)

College of Agriculture& Life Science, Dept. of Animal Biotechnology, Jeonbuk National University, Korea.

Research Area: Nutraceuticals and Nutraceutical-Derived Nanomaterials on Cancer

& Nano-nutrition on Muscle Growth (chicken embryo). Postdoctoral Researcher-(2013-2014)

College of Agriculture& Life Science, Dept. of Animal Science, Jeonbuk National University, Korea.

Research Area: Role of Bioactive Peptide from Bovine Muscle Against OS. Achievements (Two National Grant obtained as Principal Investigator-PI) 1. National Research Foundation of Korea (NRF) Ministry of Education, Science and Technology (MEST)- Ref. No: 2017R1D1A1B03032217 (2017-2020) Title: The study of in ovo feeding with nano- nutrition on muscle growth”. Fund Amount: $150,000

2. Indian Council of Medical Research (ICMR)-Senior Research Fellow (SRF) Title: Modulatory effect of galangin on NF-kB signaling and endoplasmic reticulum stress: Potential mechanisms for the insulin sensitivity effects in a rat model of metabolic syndrome.

Fund Amount: $15,000

Period: 2010-2013. Ref. No: 3/1/2/(4)/OBS/09/NCD-II 2

Skills

Material Synthesis: Experienced in optimizing and characterizing hydrogels using tensile and compressive strength tests, along with conducting in vitro biodegradation assays.

Molecular Techniques: Western blot, qRT-PCR, immunofluorescence (IF), immunohistochemistry (IHC) and flow cytometry.

Cell Culture: Proficient in isolating, purifying, and characterizing primary cells such as keratinocytes, fibroblasts, adipocytes, myoblasts, osteoblasts, and hepatocytes from human tissue, rat, and chicken tissues.

Cell-Based Assays: Experienced in Skilled in monoculture, co-culture, MTT assays, Annexin V staining for apoptosis, wound scratch and migration assays.

Analytical Techniques: Hands-on experience with FT-IR, TEM, SEM, XRD, and FE-TEM for material and sample analysis.

Animal Handling & Surgery: Worked on developing models for bone fracture, diabetes and tendon degeneration in rats and rabbits. Familiar with handling mice and Drosophila melanogaster, including oral and i.p injection.

Histology

Staining (H&E, PAS, Trichrome, Sirius Red, IHC, IF), followed by microscopy analysis. PUBLICATIONS

2025

1. Kang, H.J., Subramanian, S.A (Co-first author)., Song, S.Y., Hwang, J., Lee, C. and Kim, S.J., 2025. Epigallocatechin-3-Gallate (EGCG)-Loaded Hyaluronic Acid Hydrogel Seems to Be Effective in a Rat Model of Collagenase-Induced Achilles Tendinopathy. Journal of Functional Biomaterials, 16(2), p.55.

2. Cho, B.K., Subramanian, S.A (Co-first author)., Hwang, J., Lee, C., Yune, Y.P., Kim, S.J. and Choi, S.M., 2025. Treatment Strategy for Posterior Malleolar Fractures: Different Operative Strategies are Needed for Each Morphological Type. Journal of Clinical Medicine, 14(4), p.1216.

2024

3. Rajamohan, R., Murugavel, K., Almoajel, A., Elsadek, M.F., Subramanian, S.A., Kim, S.J., Dhandapani, S. and Lee, Y.R., 2024. Fabrication of biocompatible water-soluble supramolecular nanofibers of oseltamivir with methyl-β-cyclodextrin for enhanced drug release. Journal of Drug Delivery Science and Technology, p.105769. 4. Brindhadevi, K, Subramanian, S.A (Co-first author) Kim, P.T., Wadaan, M.A., Selvam, D.R. and Kim, S.J., 2024. Antimicrobial and anti-diabetic efficiency of Polyalthia longifolia leaf extracts and major compounds characterization. Environmental Research, 246, p.118061.

5. Rajamohan, R., Govindasamy, C., Raj, M.R., Raorane, C.J., Kim, S.C., Subramanian, S.A., Kim, S.J., Murugavel, K., Dhandapani, S. and Lee, Y.R., 2024. Loading of 4- Chloro-3-Formylcoumarin on the Potassium-Based Perylene Tetracarboxylic Acid as the Metal–Organic Framework for the Biocompatible Antimicrobial Performances. Korean Journal of Chemical Engineering, pp.1-12.

2023

6. Subramanian, S.A., Kim, H.N., Kim, S., Hwang, J., Lee, D.I., Rhim, H.C., Kim, S.J., Schon, L. and Sung, I.H., 2023. Long-Term Survival Analysis of 5619 Total Ankle Arthroplasty and Patient Risk Factors for Failure. Journal of Clinical Medicine, 13(1), p.179.

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7. Rajamohan, R., Muthuraja, P., Govindasamy, C., Subramanian, S.A., Kim, S.J., Krishnan, M.M., Murugavel, K. and Lee, Y.R., 2023. Enhanced photostability and biocompatibility of sunscreen formulation of 2-phenylbenzimidazole-5-sulfonic acid with methyl-beta-cyclodextrin. Journal of Molecular Liquids, 390, p.123013. 8. Rajamohan, R., Kamaraj, E., Govindasamy, C., Subramanian, S.A., Kim, S.J., Krishnan, M.M., Murugavel, K., Murugan, M. and Lee, Y.R., 2023. Formulation of Ensulizole with Beta-Cyclodextrins for Improved Sunscreen Activity and Biocompatibility. Journal of Pharmaceutical Sciences.

9. Mariadoss, A.V.., Subramaniyan, S.A (Co-first author) Kwon, Y.M., Shin, S. and Kim, S.J., 2023. Epigallocatechin gallate protects the hydrogen peroxide-induced cytotoxicity and oxidative stress in tenocytes. Process Biochemistry, 130, pp.105-115. 10. Mariadoss, A.V.A., Saravanakumar, K., Sathiyaseelan, A., Sivakumar, A.S., Zhang, X., Choi, H.B., Jeong, M.S., Choi, M. and Wang, M.H., 2023. Cellulose-graphene oxide nanocomposites encapsulated with green synthesized silver nanoparticles as an effective antibacterial agent. Materials Today Communications, 35, p.105652. 2022

11. Siddiqui, S.H., Subramaniyan, S.A (co-first author) Park, J., Kang, D., Khan, M., Belal, S.A., Lee, S.C. and Shim, K., 2022. Modulatory effects of cell–cell interactions between porcine skeletal muscle satellite cells and fibroblasts on the expression of myogenesis- related genes. Journal of Applied Animal Research, 50(1), pp.259-268. 12. Allur Subramanian S, Oh S, Mariadoss AVA, Chae S, Dhandapani S, Parasuraman PS, Song SY, Woo C, Dong X, Choi JY, Kim SJ. Tunable mechanical properties of Mo3Se3- poly vinyl alcohol-based/silk fibroin-based nanowire ensure the regeneration mechanism in tenocytes derived from human bone marrow stem cells. Int J Biol Macromol. 2022 Jun 15;210:196-207. doi: 10.1016/j.ijbiomac.2022.04.211. Epub 2022 May 2. PMID: 35513108.

13. Mariadoss AVA, Sivakumar AS, Lee CH, Kim SJ. Diabetes mellitus and diabetic foot ulcer: Etiology, biochemical and molecular based treatment strategies via gene and nanotherapy. Biomed Pharmacother. 2022 Jul;151:113134. doi: 10.1016/j.biopha.2022.113134. Epub 2022 May 24. PMID: 35617802. 14. Rampa, D.R., Feng, H., Allur-Subramaniyan, S., Shim, K., Pekcec, A., Lee, D., Doods, H. and Wu, D., 2022. Kinin B1 receptor blockade attenuates hepatic fibrosis and portal hypertension in chronic liver diseases in mice. Journal of Translational Medicine, 20(1), pp.1-15.

15. Parasuraman, V., Sharmin, A.M., Anand, M.A.V., Sivakumar, A.S., Surendhiran, D., Sharesh, G. and Kim, S., 2022. Fabrication and bacterial inhibitory activity of essential oil linalool loaded biocapsules against Escherichia coli. Journal of Drug Delivery Science and Technology, p.103495.

2021

16. Sivakumar Allur Subramaniyan, Naziya Begum, Sung Jae Kim, Youn Hee Choi, Taek- Jeong Nam. Biopeptides of Pyropia yezoensis and their potential health benefits: A review. Asian Pac J Trop Biomed 2021;11:375-84.

17. Siddiqui SH, Subramaniyan SA, Kang D, Park J, Khan M, Shim K. Modulatory effect of heat stress on viability of primary cultured chicken satellite cells and expression of heat shock proteins ex vivo. Anim Biotechnol. 2021 Dec;32(6):774-785. doi: 10.1080/10495398.2020.1757460. Epub 2020 Apr 27. PMID: 32340526. 2020

18. Siddiqui SH, Subramaniyan SA, Kang D, Park J, Khan M, Choi HW, Shim K. Direct exposure to mild heat stress stimulates cell viability and heat shock protein expression in primary cultured broiler fibroblasts. Cell Stress Chaperones. 2020 Nov;25(6):1033-1043. doi: 10.1007/s12192-020-01140-x. Epub 2020 Jul 22. PMID: 32696180; PMCID: 4

PMC7591668.

19. Subramaniyan SA, Kang D, Siddiqui SH, Park J, Tian W, Park B, Shim K. Effects of In Ovo Supplementation with Nano-nutrition (L-Arginine Conjugated with Ag NPs) on Muscle Growth, Immune Response and Heat Shock Proteins at Different Chicken Embryonic Development Stages. Animals (Basel). 2020 Mar 27;10(4):564. doi: 10.3390/ani10040564. PMID: 32230934; PMCID: PMC7222345. 20. Ravichandiran P, Boguszewska-Czubara A, Masłyk M, Bella AP, Johnson PM, Subramaniyan SA, Shim KS, Yoo DJ. A phenoxazine-based fluorescent chemosensor for dual channel detection of Cd2+ and CN− ions and its application to bio-imaging in live cells and zebrafish. Dyes and Pigments. 2020 Jan 1;172:107828. 2019

21. Tripathi VK, Subramaniyan SA, Hwang I. Molecular and Cellular Response of Co- cultured Cells toward Cobalt Chloride (CoCl2)-Induced Hypoxia. ACS Omega. 2019 Dec 2;4(25):208**-*****. doi: 10.1021/acsomega.9b01474. PMID: 31867478; PMCID: PMC6921254.

22. Ravichandiran, Palanisamy; Boguszewska-Czubara, Anna; Masłyk, Maciej; Subramaniyan, Sivakumar Allur; Bella, Antony Paulraj; Johnson, Princy Merlin; Shim, Kwan Seob; Yoo, Dong Jin. A Naphthoquinone-Based Colorimetric and Fluorometric Dual Channel Chemosensor for the Detection of Fe2+ Ion and its Application in Bio- Imaging of Live Cells and Zebrafish. Submitted to ACS Sustainable chemistry & Engineering. 2019; doi.org/10.1021/acssuschemeng.9b03822. 23. Ravichandiran P, Subramaniyan SA, Bella AP, Johnson PM, Kim AR, Shim KS, Yoo DJ. Simple fluorescence turn-on chemosensor for selective detection of Ba2+ ion and its live cell imaging. Analytical chemistry. 2019 Jun 28;91(15):10095-101. 24. Subramaniyan SA, Kang DR, Park JR, Siddiqui SH, Ravichandiran P, Yoo DJ, Na CS, Shim KS. Effect of In Ovo Injection of L-Arginine in Different Chicken Embryonic Development Stages on Post-Hatchability, Immune Response, and Myo-D and Myogenin Proteins. Animals (Basel). 2019 Jun 14;9(6):357. doi: 10.3390/ani9060357. PMID: 31207968; PMCID: PMC6617498.

25. P. Ravichandiran, Sivakumar Allur Subramaniyan, Seon-Young Kim, D. Premnath, Jong-Soo Kim, Byung-Hyun Park, Kwan Seob Shim. Synthesis and anticancer evaluation of novel 1,4-naphthoquinone derivatives containing a phenylamino-sulfanyl moiety. ChemMedChem. 2019; 14: 532-544, DOI.org/10.1002/cmdc.201800749. 2018

26. Sheet S, Vinothkannan M, Balasubramaniam S, Subramaniyan SA, Acharya S, Lee YS. Highly Flexible Electrospun Hybrid (Polyurethane/Dextran/Pyocyanin) Membrane for Antibacterial Activity via Generation of Oxidative Stress. ACS Omega. 2018 Nov 30;3(11):145**-*****. doi: 10.1021/acsomega.8b01607. Epub 2018 Nov 1. PMID: 30555979; PMCID: PMC6289494.

27. Belal SA, Sivakumar AS, Kang DR, Cho S, Choe HS, Shim KS. Modulatory effect of linoleic and oleic acid on cell proliferation and lipid metabolism gene expressions in primary bovine satellite cells. Anim Cells Syst (Seoul). 2018 Sep 9;22(5):324-333. doi: 10.1080/19768354.2018.1517824. PMID: 30460114; PMCID: PMC6171434. 28. Allur Subramaniyan S, Sheet S, Balasubramaniam S, Berwin Singh SV, Rampa DR, Shanmugam S, Kang DR, Choe HS, Shim KS. Fabrication of nanofiber coated with l- arginine via electrospinning technique: a novel nanomatrix to counter oxidative stress under crosstalk of co-cultured fibroblasts and satellite cells. Cell Commun Adhes. 2018 Dec;24(1):19-32. doi: 10.1080/15419061.2018.1493107. Epub 2018 Sep 5. PMID: 30182742.

29. Belal SA, Kang DR, Sivakumar AS, Choe HS, Shim KS. Effect of long chain fatty acids on triacylglycerol accumulation, fatty acid composition and related gene expression in 5

primary cultured bovine satellite cells. Anim Biotechnol. 2019 Oct;30(4):323-331. doi: 10.1080/10495398.2018.1496925. Epub 2018 Sep 4. PMID: 30179065. 30. Sivakumar AS, Tripathi VK, Dhasmana A, Hwang I. Crosstalk Between Co-Cultured 3T3-L1 and C2C12 Cells After the Exposure of Nano-Titanium Dioxide. J Nanosci Nanotechnol. 2018 Jun 1;18(6):3870-3879. doi: 10.1166/jnn.2018.15184. PMID: 29442721.

31. Subramaniyan SA, Sheet S, Vinothkannan M, Yoo DJ, Lee YS, Belal SA, Shim KS. One-Pot Facile Synthesis of Pt Nanoparticles Using Cultural Filtrate of Microgravity Simulated Grown P. chrysogenum and Their Activity on Bacteria and Cancer Cells. J Nanosci Nanotechnol. 2018 May 1;18(5):3110-3125. doi: 10.1166/jnn.2018.14661. PMID: 29442810.

32. Allur Subramaniyan, S., Kang, D.R., Belal, S.A. et al. A Comparative Study of Biologically and Chemically Fabricated Synthesized AgNPs’ Supplementation with Respect to Heat-Shock Proteins, Survival, and Hatching Rates of Chicken Embryos: An In Ovo Study. J Clust Sci 29, 129–139 (2018). doi.org/10.1007/s10876-017-1319-5 2017

33. Subramaniyan SA, Hwang I. Biological Differences between Hanwoo longissimus dorsi and semimembranosus Muscles in Collagen Synthesis of Fibroblasts. Korean J Food Sci Anim Resour. 2017;37(3):392-401. doi: 10.5851/kosfa.2017.37.3.392. Epub 2017 Jun 30. PMID: 28747825; PMCID: PMC5516066.

34. Sheet S, Sathishkumar Y, Sivakumar AS, Shim KS, Lee YS. Low-shear-modeled microgravity-grown Penicillium chrysogenum-mediated biosynthesis of silver nanoparticles with enhanced antimicrobial activity and its anticancer effect in human liver cancer and fibroblast cells. Bioprocess Biosyst Eng. 2017 Oct;40(10):1529-1542. doi: 10.1007/s00449-017-1809-8. Epub 2017 Jul 14. PMID: 28710569. 35. Sivakumar Allur Subramaniyan, Da Rae Kang, Young Chul Jung, Jong Hyun Jung, Yang Il Choi, Mun Jun Lee, Ho Sung Choe, D, Kwan Seob Shim. Comparative studies of meat quality traits and the proteome profile between low pH and high pH muscles in longissimus dorsi of Berkshire. Canadian Journal of Animal Science. 2017; 97: 640– 649. DOI 10.1139/CJAS-2016-0230.

36. Sivakumar AS, Krishnaraj C, Sheet S, Rampa DR, Kang DR, Belal SA, Kumar A, Hwang IH, Yun SI, Lee YS, Shim KS. Interaction of silver and gold nanoparticles in mammalian cancer: as real topical bullet for wound healing- A comparative study. In Vitro Cell Dev Biol Anim. 2017 Aug;53(7):632-645. doi: 10.1007/s11626-017-0150-5. Epub 2017 May 1. PMID: 28462492.

2016

37. Subramaniyan SA, Kang DR, Belal SA, Cho ES, Jung JH, Jung YC, Choi YI, Shim KS. Meat Quality and Physicochemical Trait Assessments of Berkshire and Commercial 3- way Crossbred Pigs. Korean J Food Sci Anim Resour. 2016 Oct 31;36(5):641-649. doi: 10.5851/kosfa.2016.36.5.641. PMID: 27857540; PMCID: PMC5112427. 38. Ramachandran R, Krishnaraj C, Sivakumar AS, Prasannakumar P, Abhay Kumar VK, Shim KS, Song CG, Yun SI. Anticancer activity of biologically synthesized silver and gold nanoparticles on mouse myoblast cancer cells and their toxicity against embryonic zebrafish. Mater Sci Eng C Mater Biol Appl. 2017 Apr 1;73:674-683. doi: 10.1016/j.msec.2016.12.110. Epub 2016 Dec 23. PMID: 28183660. 39. Subramaniyan SA, Kim S, Hwang I. Cell-Cell Communication Between Fibroblast and 3T3-L1 Cells Under Co-culturing in Oxidative Stress Condition Induced by H2O2. Appl Biochem Biotechnol. 2016 Oct;180(4):668-681. doi: 10.1007/s12010-016-2123-7. Epub 2016 May 18. PMID: 27193258.

2014-2015

40. Sivakumar AS, Hwang I. Effects of Sunphenon and Polyphenon 60 on proteolytic 6

pathways, inflammatory cytokines and myogenic markers in H2O2-treated C2C12 cells. J Biosci. 2015 Mar;40(1):53-9. doi: 10.1007/s12038-015-9503-y. PMID: 25740141. 41. Venkata Reddy B, Sivakumar AS, Jeong DW, Woo YB, Park SJ, Lee SY, Byun JY, Kim CH, Cho SH, Hwang I. Beef quality traits of heifer in comparison with steer, bull and cow at various feeding environments. Anim Sci J. 2015 Jan;86(1):1-16. doi: 10.1111/asj.12266. Epub 2014 Sep 19. PMID: 25236779. 42. Sivakumar AS, Ochirbat C, Cho SH, Yang J, Hwang I. Antiapoptotic effect of a novel synthetic peptide from bovine muscle and MPG peptide on H2O2-induced C2C12 cells. In Vitro Cell Dev Biol Anim. 2014 Aug;50(7):630-9. doi: 10.1007/s11626-014-9745-2. Epub 2014 May 14. PMID: 24825375.

43. Sivakumar AS, Anuradha CV. Effect of galangin supplementation on oxidative damage and inflammatory changes in fructose-fed rat liver. Chem Biol Interact. 2011 Sep 5;193(2):141-8. doi: 10.1016/j.cbi.2011.06.003. Epub 2011 Jun 25. PMID: 21708140. 44. Kaviarasan S, Sivakumar A.S, Barik A, Kunwar A, Naik GH, Priyadarsini K.I. Potent radical scavenging ability of Sunphenon: a green tea extract. Journal of Food Biochemistry. Volume 35, Issue 2, pages 596–612, April 2011. 45. Sivakumar AS, Viswanathan P, Anuradha CV. Dose-dependent effect of galangin on fructose-mediated insulin resistance and oxidative events in rat kidney. Redox Rep. 2010;15(5):224-32. doi: 10.1179/135100010X12826446921545. PMID: 21062538; PMCID: PMC7067339.

References:

1. Prof. C. V. Anuradha

Professor,

Dept. of Biochemistry and Biotechnology,

Annamalai University

Tamil Nadu, India. Ph: Off: 041**-******, Res: 041**-******, Email: ********@*******.***

2. Prof. Kwan-Seob Shim, Ph.D.

Associate Professor, Dept. of Animal Biotechnology, College of Agriculture and Life Science,

Jeonbuk National University,

#664-14 Duckjin-dong 1Ga, Jeonju City, Jeonbuk 561-756 Republic of Korea. Tel: +82-63-270-****; Fax: +82-63-270-**** Email: ******@****.**.** or ********.****@*****.*** 3. Prof. Inho Hwang

Dept of Animal Science,

Colleges of Agriculture and Life Sciences

Jeonbuk National University, Jeonju, 561-756

South Korea

Mobile: +821*-****-****

E.mail: ****.*****@****.**.**

Yours truly

(A.S. SIVAKUMAR)

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