Quality Assurance Wet Chemistry
Resume:
Christopher Puls
Elkridge Md 21075
**********@*****.***
Objective
A challenging position where I can use my education and skills in a science environment with emphasis on career fulfillment.
Specialties
Atomic absorption
Wet chemistry
Good Manufacturing Practices (cGMP)
Experience
Principal Technician, April 2002-2025
MRI Global, Gaithersburg, Maryland
Responsible person for the Reagent Prep Department and for equipment temperature logs, reagent prep and aliquoting, RODAC plating and reading, making and aliquoting HIBAAAUS, HIBAAAUS-HS making and aliquoting phosphate-buffered saline/Tween, phosphate-buffered saline/Triton. aliquoting AE Buffer, AL Buffer, Elution Buffers, Protienase K buffer. Prep of Bead Beating Tubes Autoclaving DI water and waste, and in processing of samples. Checking Calibration on centrifuges, thermomixers and thermometers for Quality Assurance Department. Purification Specialist, 2000-2002
Cambrex Bio Science, Baltimore, Maryland
Trained in current Good Manufacturing Practices (cGMP). Biomanufacturing Clean room experience BSL-2, BSL-3. Experienced in purifying Replagal Enzyme Replacement Therapy for patients with Fabry disease, an inherited rare genetic disorder. Fermentation Experience operating Amicon K-Prime 40 II chromatography system. Electron Microscope Experienced in packing, unpacking, and cleaning chromatography columns. Experience with Pall Filtron open channel centrasett filters. SOP development.
Chemist, 1996-2000
Severn Trent Laboratories, Sparks, Maryland
Operated Perkin-Elmer 1600 FTIR and conducted various wet chemistry methods: biochemical oxygen demand, chemical oxygen demand, flash point, hardness, hexavalent chromium, ignitability, oil and grease, pH, total petroleum hydrocarbons, total solids, total dissolved solids, total suspended solids, sulfate, and turbidity.
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