Research Associate Senior Technical
Resume:
VICTOR D. KOREN’KOV, Ph.D.
Lexington, KY 40503
Phone: 859-***-**** cell
E-mails : *******@***.***
*********@*******.***
OBJECTIVE
Research Associate, Post-Doctoral Scholar, Scientific, or Senior Technical Position in the fields of Biochemistry, Plant Physiology, or Field Biology with a university, company or organization seeking an individual who is motivated, dependable, energetic, knowledgeable, has excellent analytical and organizational skills, and is excited about learning new methods and techniques in life science and environmental studies. With a proven record of scientific vision and discovery, I have consistently demonstrated the ability to lead impactful research and contribute significantly to the advancement of the above fields.
AREAS OF EXPERTISE
I have had fundamental research and diverse lab experience over twenty years in the Department of Plant and Soil Sciences at the University of Kentucky (Plant Physiology/ Biochemistry/Molecular Biology Program).
I have studied mechanisms that control ion (Cd, Zn, Mn, Ca) accumulation and tissue partitioning in plants using a wide array of genetic engineering techniques which modify membrane transporters to improve ion selectivity and transport efficiency. This is a key factor in efficient manipulation of plant ion transport for the purpose of enhancing quality and safety of food and providing soil phytoremediation. I have studied the impact of plant cell transporters and their modification on plant tolerance to heavy metal stress. I have applied the above skills when conducting four years of field trials aimed at analyzing transgenic and non transgenic plants that have potential to decrease the amount of pollutant in plant tissues used by humans.
Studied the mechanisms by which LWW, AMP and Au and AgNPs could influence membrane integrity and cause membrane perturbation. We found that ZnNPs inhibited spore germination and spore infectivity in different plants. We studied composition of aroma and other compounds in plants by Gas Chromatography/Mass Spectrometry which allowed us to determine medical and aroma/flavor values of these compounds (TMS and BE studies). Preparation of butyl ester derivatives of SE acyl groups and GC-MS analysis. Participated in the experiments aimed at decreasing harmful effect of tobacco product (Thermal Separation Probe (TSP) in GC/MS research).
Studied trichrome exudates on different tobacco varieties ( by TMS analysis) in view of determine CBT-diols and other terpene and terpenoids compounds in these plants. Worked intensively (by TMS GC/MS analysis) on Artemisia plant to determine its medical, and flavor and aroma values under different temperatures.
LABORATORY / RESEARCH
Conduct numerous experiments with GAS Chromatography/Mass Spectrometry with different plant samples and exudates under different experimental conditions
Expert in research instrumentation such as double-wavelength, double-beam spectrophotometer and fluorescence spectrophotometer.
Highly skilled in preparation of vacuoles and vacuolar membranes from different kinds of plant species. Performed assay of ATP and PPi hydrolysis and proton translocation activity across the tonoplast using pH and probes.
Experienced in transport research across biological membranes with labeled compounds (transport with 109Cd, 45Ca, 63Ni, 54Mn, 86Rb,14C-methylamine).
Developed the methods of incorporation of chelator protein metallothionein into tonoplast membranes and artificial proteoliposomes to study plant tolerance to toxic metals.
Extensive experience with solution culture and field trial experiments related to the problem of tolerance and phytoremediation with normal and transgenic tobacco plants.
Experienced in working with transgenic tobacco plants: developed methods of isolation of tonoplast membranes from roots of different transgenic tobacco species and studied ion transport activity across the tonoplast and artificial proteoliposomes.
Worked on fungi culture while sustaining this culture in vitro.
Investigated the mechanisms of fungal inhibition and mechanisms of plant defense against pathogen.
Worked with plant transplants in vitro.
Experienced in membrane labeling: developed method of tonoplast labeling with 3H-azidopine and 3H-verapamil.
Strong technical writing skills. 35 publications, including in peer-reviewed journals.
FIELD EXPERIENCE
Research Associate and Staff Member in Plant and Soil Sciences Department at the University of Kentucky, Plant Physiology/Biochemistry/Molecular Biology Program, Lexington, Kentucky. Lab Head and PI Professor G.J. Wagner and Ph.D, Scientist III - Antoaneta Kroumova (July 2009 -present)
Investigated chemistry of trichome exudates from different types of tobacco and Artemisia plants for their insecticidal, antibiotic/medical properties, and potential for insect and disease resistance properties.
Studied the effect of tobacco leaf water wash (LWW) and antimicrobial proteins (AMP) on membrane integrity and permeability. Showed that LWW, polymyxin, nisin, glycophorin cause proton, potassium, rubidium leakage from the tonoplast and artificial liposomes.
Showed that LWW and AMP cause proton leakage and NRed loss from the Blue mold spores.
Examined the NPs effect on fungal, plant cell and artificial membrane integrity and perturbations .We found ZnNP, AuNPs and AgNPs inhibited spore germination and plant leaf infectivity
Research Associate in Plant and Soil Sciences Department at the University of Kentucky, Plant Physiology/Biochemistry/Molecular Biology Program, Lexington, Kentucky. Lab Head Professor G.J. Wagner (July 2004 - April 2008)
Found that plants expressing CAX genes have higher potential to accumulate more Cd, Zn and Mn due to increased activity of tonoplast located Me/H antiporters or exchangers.
Studied tobacco plant tolerance to increased Cd, Zn, Mn concentration. Revealed the factors which are responsible for higher root or shoot accumulation of these elements and factors responsible for tolerance. Conducted field studies to reduce the amount of unwanted pollutant in tissues used by humans.
Post-doctoral and full-time visiting researcher in Agronomy Department at the University of Kentucky, Lab of Professor G.J. Wagner (November 1996-June 2000, July 2001-2004):
Studied and compared 109Cd, 45Ca, 63Ni transport mechanism in tonoplast from oat and tobacco roots. Reconstituted tonoplast Cd and Ca transport activities into artificial proteoliposomes from different plant species.
Studied transport of Cd, Ca, Zn, Mn in the tonoplast in transgenic tobacco plants and reconstituted proteoliposomes from different type of transgenic tobacco plants (CAX1, CAX2, CAX3, CAX4 expressing).
Researcher and Senior Scientific Officer in the Laboratory of Plant Cell Membranes at K.A.Timiriazev Institute of Plant Physiology Moscow, Russia (1979-1993, 1994-1996, 2000-2001):
Studied mechanisms of tonoplast transport processes. Identified and described the properties of the primary (H+-translocating ATPase and PPase) and secondary (Ca2+/H+, sucrose/H+, arginine/H+ antiporter) tonoplast transport systems.
Revealed the factors such as calmodulin, osmotic factor, ATP and other nucleotides which could be involved in the control and modulation of the activity of ion and metabolites transport systems on the tonoplast.
Worked on energy-dependent transport of ions and metabolites across peribacteroid membrane of Lupine (Lupinus luteus L.).
Fellow Researcher, Rothamsted International Rothamsted Experimental Station, Physiology and Biochemistry Department (Head - Professor Roger A. Leigh) Harpenden, United Kingdom (November 1993-November 1994):
Studied the properties of tonoplast-bound PPase from mung bean, oat and beet roots and investigated the factors (substrates, effectors and inhibitors) which can control tonoplast-bound H+-pyrophosphatase.
EDUCATION Bachelor and Master of Science in Biology, June 1979
Biochemistry Department
Moscow Agricultural Academy
Moscow, Russia
DEGREE Ph.D. in Biology May 1992
Thesis: Ion Transport Systems of the Tonoplast
from Pea Leaves Mesophyll
The thesis was defended at K.A.Timiriazev Institute of Plant Physiology. Russian Academy of Sciences
Moscow, Russia
SKILLS MS Word, Excel, PowerPoint, Adobe Photoshop, Sigma Plot
EMPLOYMENT Department of Plant and Soil Sciences
Plant Physiology/Biochemistry/Molecular Biology Program
University of Kentucky, Lexington, Kentucky:
oResearch Associate, Staff- July 2004 - 2024
oFull-Time Visiting Researcher, July 2001 - June 2004
oPostdoctoral Researcher, November 1996 - June 2000
K. A. Timiriazev Institute of Plant Physiology
Plant Cell Membranes Laboratory
Russian Academy of Sciences, Moscow, Russia:
oScientific Officer, Senior Scientific Officer,
November 1979 - October 2006
AWARDS Calcium Exchange Expression in Plants, Disclosure of Invention Tamus 1684 (with Hirschi K.D., Wilganowski N, Wagner G.J.), 2001
Rothamsted International Fellowship, IACR Rothamsted
Harpenden, Hertfordshire, United Kingdom
November 1993 - November 1994
MEMBERSHIPS American Society of Plant Biologists,
June 1999 - Present
PUBLICATIONS more than 30 publications in western and other journals including
peer-reviewed. A list of the main recent publications attached.
REFERENCES Dr. George J. Wagner
Plant Physiology/Biochemistry/Molecular Biology Program
Room 117 L, KTR&DC
Cooper and University Drives
University of Kentucky
Lexington, KY 40546-0236
Tel: 859-***-****
Fax: 859-***-****
E-mail *******@***.***
Dr. Roger A. Leigh
Department of Plant Science
University of Cambridge
Downing Street
Cambridge CB2 3EA
United Kingdom
Tel: +44(0) 122*-******
Fax: +44(0) 122*-******
E-mail: *****.*****@********.***.**.**
Dr. Kendal Hirschi
Department of Pediatrics
Baylor College of Medicine
1100 Bates Street
Houston, Texas 77030-2600
*******@***.***.***
PRESENTATIONS
Seminars of Plant Biochemistry and Soil and Environmental Chemistry labs. Presentation:
Impact of AuNPs on proton leakage from membrane vesicles. University of Kentucky
March and September 2012.
61st Tobacco Science Research Conference. Symposial Talk: The Impact of Arabidopsis CAX Gene Expression on Leaf Cadmium Accumulation in Field Grown Tobacco. Charlotte, NC, September 23-26, 2007
KTR&DC Seminar - Effect of Arabidopsis CAX Genes Expression on Tolerance of Tobacco to Growth with High Cd, Zn and Mn. University of Kentucky, Lexington, KY
June 2007
Seminar at Baylor College of Medicine, Dr. K. Hirschi’s lab. Influence of CAX Genes on Vacuolar Transporters and Tolerance to Heavy Metals, Houston, TX, October 2006
Fourth Philip Morris USA Genomics Mini-Symposium, co-author of symposial talk. Richmond, VA June 21-23, 2005
Philip Morris USA Genome Research Project Review Meeting, co-author of symposial talk. Richmond, VA, June 12-14, 2003
56th Tobacco Science Research Conference, symposial talk: Approaches for Studying Cd, Ca and Mn Accumulation in Control and Transgenic Tobacco Plants Expressing CAX Genes. Lexington, KY, September 29-October 2, 2002
First Philip Morris USA Genomics Mini-Symposium, symposial talk. Richmond, VA, June 25 -26, 2002
Organizer of International Symposium - Plant under Environmental Stress, Moscow, October 23-28, 2001
Participated in ASPB 75th-year Meeting. Membrane Transport Session No. 47, poster 746: Comparison of Cd and Ca transport activities in tonopalst and proteoliposomes from root of oat seedlings and mature tobacco roots. Baltimore, MD, July 1999
PUBLICATIONS
1.Antoaneta Mihaylova-Kroumova, Victor D. Korenkov, George Wagner, Jeffery Kinney – Exploring the Potential of Artemisia annua L. for Recreation and Therapeutic Uses in Heated Tobacco Devices – in preparation 2004, Herbs, Spices and Medicinal Plants
2.Antoaneta Mihaylova-Kroumova, George J. Wagner, Victor D. Korenkov –Characterization of volatiles from heated reference cigarettes 3R4F using thermal separation probe GC/MS.-77th TRSC, 2024
3.Antoaneta B. Mihaylova-Kroumova, Ivan Artiouchine, Victor D. Korenkov & George Wagner -Patterns of inheritance of acylsugar acyl groups in selected interspecific hybrids of genus Nicotiana – Journal of Plant Research, 2020, v.133, p. 509–523
4.George Wagner, Victor Korenkov, Jonathan D. Judy, Paul M. Bertsch – Nanoparticles Composed of Zn and ZnO Inhibit Peronospora tabacina Spore Germination in vitro and P.tabacina Infectivity on Tobacco leaves - Nanomaterials 2016, 6(3), 50; https://doi.org/10.3390/nano6030050
5.Korenkov V, King B, Hirschi K, Wagner GJ - Root selective expression of AtCAX4 and AtCAX2 results in reduced lamina Cd in field grown Nicotiana tabacum L. – Plant Biotechnology Journal, 2009, v.7, p.219-226.
6.Korenkov V, Hirschi K, Crutchfield JD, Wagner GJ - Enhancing tonoplast Cd/H antiport activity increases Cd, Zn and Mn tolerance, and impact root/shoot Cd partitioning in Nicotiana tabacum L. Planta, 2007, v.226, p.1379-1387.
7.Korenkov V, King B, Wagner GJ, Hirschi K - The impact of Arabidopsis CAX gene expression on leaf cadmium accumulation in field grown tobacco. 61st Tobacco Science Research Conference, Charlotte, NC, 2007.
8.Koren’kov V, Park S, Cheng N-H, Sreevidya J, Lachmansingh C, Morris J, Hirschi K, Wagner GJ - Enhanced Cd2+-selective root-tonoplast-transport in tobaccos expressing Arabidopsis cation exchangers. Planta, 2007, v.225, p. 403-411.
9.Wagner G, Korenkov V, Hirschi K et al. - Potential of Transgenic Plants for Reducing the Cadmium content of food and tobacco products. Acta Tabacaria Sinica, 2006, v.12, No.2.
10.Koren’kov V, Pittman J, Hirschi K, Wagner G - Comparison and characterization of root tonoplast H/divalent catioin activities in AtCAX2 and CAX4 Expressing Tobacco Abstract of Annual Meeting of ASPP, Orlando, FL, July 2004.
11. Koren'kov V, Shepherd RW and Wagner GJ - The use of reconstitution and inhibitior/ion interaction assay to distinguish between Ca/H and Cd/H antiporter activities of oat and tobacco tonoplast vesicles. Physiologia Plantarum, 2002, v.116, p.359-367.
12.Koren’kov V, Wagner GJ - Approaches for studying cadmium, calcium and manganese accumulation in control and transgenic tobacco plants expressing CAX genes. Abstracts of 56th Tobacco Science Research Conference, September 29-October 2, 2002, Lexington, KY.
13.Koren’kov V, Hirschi K, Wagner G - Exploiting Arabidopsis thaliana CAX genes for reducing leaf cadmium content in tobacco: strategy and biochemical aspects. Abstracts of First Philip Morris USA Genomics Mini-Symposium, June 25-26, Richmond, VA, 2002, p.17.
14.Koren’kov V, Hirschi K, Wagner G - Tonoplast antiporters: reconstitution into proteoliposomes and enhanched activity in transgenic plants. Abstracts of International Symposium - Plant under Environmental Stress, October 23-28 Moscow, Russia, 2001.
15.Hirschi, K.D., Koren'kov, V, Wilganowski, N, Wagner, G - Expression of Arabidopsis CAX2 in tobacco: Altered metal accumulation and increased manganese tolerance. Plant Physiology, 2000, v.124, p.125-133.
16.Wagner, G, Koren'kov, V - Isolation of ion-transport -competent vesicles from tobacco roots. - Abstract of 1999 Agro & Phyto Joint Meeting. 10-14 October 1999, Suzhou, China.
17.Koren’kov, V, Gonzales, A, Wagner, GJ - Comparison of Cd and Ca transport activities in tonoplast and proteoliposomes from roots of oat seedlings and mature tobacco plants. Abstract of Annual Meeting of ASPP, p.158, p.746, Baltimore, MD, USA, July 1999.
18.Gonsales-Mejia, A., Koren'kov, V and Wagner, GJ - A Comparison of Zn, Mn, Cd and Ca transport mechanisms in oat root tonoplast vesicles. Physiologia Plantarum 1999, v. 106, p. 203-209.
19.Gordon-Weeks, R., Koren'kov, V.D., Steele, S.H. & Leigh, R.A. - Tris is a competitive inhibitor of K+ activation of the vacuolar H+-pumping pyrophosphatase. Plant Physiology, 1997, v.114, p.901-905.
20.Gordon-Weeks, R., Koren'kov, V.D., Steele S.H. and Leigh, R.A. - Tris inhibits K+-stimulation of the vacuolar H+-pumping inorganic pyrophosphatase. Plant Physiology, Supplement 1997, p.29, N.56.
21.Andreev, I.M., Dubrovo, P., Krylova, V., Andreeva, I.N. Koren'kov, V.D., Sorokin, E.M., Izmailov, S.F. - Characterization of ATP-hydrolyzing and ATP-driven proton-translocating activities associated with the peribacteroid membrane from root nodules of Lupinus luteus L. Journal of Plant Physiology, 1997, v.151, p.563-569.
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