Medical Laboratory Research Technologist

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Maryam Khani, MSc

Tehran, Iran. +98-936******* on Skype **************@*****.***,

******.*****@****.**.**

SUMMARY

• M.S. with 4+ years’ experience in BSL-2 lab, extensive experience with molecular assays, cell assays, and primary cell culture.

• Excellent organizational, analytical and technical skills to execute effectively on complex projects, and meet tight timelines.

• Outstanding interpersonal and communication skills.

• Troubleshooting skills (A published article + A thank you email from the kit manufacturer team). RESEARCH EXPERIENCE

• Shahid Beheshti Medical School, Tehran, Iran 2017 Research Technologist, Infertility and Reproductive Health Research Center The lead researcher in the project of investigation of Alu247 and Alu115. Identified protocol weaknesses, troubleshot various experiments and assays, and prioritized resources among multiple tasks.

Managed complex resources including paperwork, ordering, protocols, SOPs, and experiment materials. Executed data synthesis, collection, analysis, and interpretation. Wrote reports and presented results.

• Shahid Beheshti Medical School, Tehran, Iran 2016 Research Technologist, Urogenital Stem Cell Research Center Investigation of several long-non-coding polymorphisms in prostate cancer projects. Supported multiple projects by performing various assays, 1000+ blood samples, and 100+ tissue samples.

• Life Research Journal, ISSN 2624-0548. 01/2021-01/2022 Reviewer

• Personal Project (in processing) 2023-…

Pedigree analysis and its familial neurological disorders. Collecting of the medical history of families, Reviewing articles, …

EDUCATION

• Shahid Beheshti University (Rank #1 Medical University in Iran), GPA: 17.87/20 (3.574/4). Tehran, Iran Master of Science, Human Genetics 09/2014-09/2017

Master thesis: Investigating the relationship between circulating cell-free DNA concentration and integrity ccfDNA (circulating cell-free DNA) with prostate cancer in a group of Iranian men referring to Shahid Beheshti University of Medical Sciences.

• Shahid Beheshti University, GPA: 18.44/20 (3.688/4), (The top student). Tehran, Iran Bachelor of Science, Medical Laboratory Science 09/2012-09/2014

• Aja University Tehran, Iran

Associate of Science, Medical Laboratory Technology 09/2007-09/2009 2

SELECTED PUBLICATIONS

1. Investigating the relationship between ccfDNA concentration, its integrity, and some individual factors in an Iranian population, M. Khani et al. (Presented at the IRHRC Tehran, Iran). 2. The Value of plasma circulating cell-free DNA concentration and integrity index as a clinical tool for Prostate cancer diagnosis: A prospective case-control cohort study in an Iranian Population, M. Khani et al. (Presented at the IRHRC Tehran, Iran).

3. Evaluation of Extracted Circulating Cell-free DNA Concentration by Standard NucleoSpin Plasma XS (NS) Kit Protocol Compared to its Modified Protocol, Khani M, et al. 4. Full publication list at https://scholar.google.com/citations?user=dl8tl0MAAAAJ&hl=fa&oi=ao LABORATORY TECHNIQUES

Biochemical assays ELISA, Protein assays, Western Blot, HPLC operation, Atomic Absorption/ Flame Spectroscopy, Chromatography

Cell culture Suspension culture, Lymphocyte culture (RPMI), Microorganisms culture. Molecular biology RT-qPCR, RNA isolation, DNA isolation, NA Treatment Microscopy Phase-contrast, Bright field, IF-microscopy (immunofluorescence staining), ANA assay Immunohistochemistry.

Other lab skills Clinical laboratory microscopic and macroscopic, manual and automated techniques:

Phlebotomy. Hematology: Complete Blood Count, Differential microscopic identification of normal and blast cells, Immunocytochemistry, CT, BT, XIII, PT, PTT, Blood Bank. Microbiology: Bacteriology (staining and microscopy, culture media preparing, culture techniques, biochemical methods, BacTec, Vitek, molecular analysis), Mycology, Parasitology, Virology (molecular analysis, virus hybridization, virus typing). Histology: Fixation, Embedding, Sectioning, Staining. Urinalysis. Semen Analysis, SDFA: Sperm DNA Fragmentation Assay. General skills: Titration, Solution preparation, Standardization, Calibration, Quality Control. OTHER SKILLS

Software Microsoft Office, GraphPad Prism, ImageJ, Primer Design Software: Gen Runner, Perlprimer, IlluQC, SPSS (statistical Software), Web Sites: NCBI - www.ncbi.nlm.nih.gov, UCSC - genome.ucsc.edu, primer1, neb cutter, miRbase, optimized crispr design, sgRNA scorer, sgRNA designer, chopchop web tool, GeneScript, ATUM, CRISPOR, crispr Rgen tools.

Languages English: fluent, Persian: native, Arabic: intermediate. Referees:

Dr. Abolfazl Movafagh **********@*****.***, ********.*@****.**.** Dr. Farkhondeh Pouresmaeili *********@*****.***

Dr. Reza Mirfakhraie ****************@*****.***

Dr. Hossein Darvish **********@*****.***

Dr. Saeed Zarein Dolab ******@*****.***, *******@****.**.** Award: Supreme and Talent Student Award at the Ninth Shahid Motahari Educational Festival of Shahid Beheshti University of Medical Sciences Date: May 2014

Personal skills: Physical fitness, Chess, Poetry writing ️, Drawing and Painting .

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