Analytical Chemist

Anjam Sadik

314-***-**** ● **********@*****.*** ● LinkedIn ● Google scholar

HIGHLIGHTS

● PhD analytical chemist with 1 year of cGMP experience as a Quality Control Analyst, 7+ years of research experience, leading to 1 peer-reviewed research article, 4 conference presentations, and 2 manuscript reviews

● 6+ years of expertise in operating and maintaining analytical instruments, including LC-MS, GC-MS and CE EDUCATION

● PhD (Analytical Chemistry), University of Kentucky, KY Aug 2020 - April 2025

● MS (Analytical Chemistry), Saint Louis University, MO Aug 2016 - May 2019

● BS (Chemistry), University of Dhaka, Bangladesh Jan 2011 - Dec 2015 INDUSTRIAL EXPERIENCE

Quality Control (QC) Chemist, Huvepharma Inc., Saint Louis, MO July 2019 - July 2020

- Conducted analytical tests on active pharmaceutical ingredients (API), finished products and stability samples according to standard operating procedures (SOP) developed by United States Pharmacopeia (USP)

- Performed HPLC assay, UV-Vis, FTIR, TLC, viscosity, titration, conductivity and other wet chemistry techniques

- Ensured adherence to GLP and cGMP guidelines and documented test results, OOS and OOT INSTRUMENTAL and ANALYTICAL SKILLS

● HPLC-MS/MS (QqQ, ion trap) ● GC-MS ● Orbitrap HRMS ● MALDI-TOF MS ● UV-Visible spectroscopy

● Capillary electrophoresis (CE) ● Method development and validation ● LIMS (Sapio) ● SOP development

● Maintenance (LC-MS and GC-MS method transfers, mass spectrometric calibration, filament replacement, septum and liner replacement, GC-MS column change and ion-source cleaning) LC and LC-MS EXPERIENCE

● HPLC method development and sorbent selection for recovery of therapeutic phenolic compounds from industrial biorefinery plant biomass process streams (June 2021 – July 2024)

- Characterized 6 chromatographic packing materials (including C18 and β cyclodextrin) using LC MS on a Thermo linear ion trap platform, assessing separation performance to guide stationary phase selection

- Established retention trends for 22 phenolic monomers and dimers on C18 and β cyclodextrin columns by analyzing retention factors and selectivity with an Agilent HPLC-diode array detector (DAD) system

- Conducted preparative-HPLC with UV-vis detection to purify synthetic phenolic dimer (achieved ~100% purity) Outcomes: - HPLC separation conditions were established for selective separation of the phenolic analytes

- Characterized pressure-induced morphological changes in capillary HPLC column packing particles supporting the design of packing materials

- Demonstrated LC with fluorescence detection as a more reproducible platform for phenolic quantification ( 3 % RSD) than GC, while noting GC’s superior peak capacity

● Performed routine cGMP HPLC analyses, validating the identity and purity of pharmaceutical raw materials and finished drugs against USP specifications, averaged 15 samples per day (July 2019 – July 2020)

● LC-MS-based metabolic profiling for diabetes diagnosis, monitoring and treatment (Jan – Dec 2018)

- Quantified 20+ human TCA cycle phosphometabolites and organic acids via targeted tandem mass spectrometry on a Thermo triple quadrupole LC-MS (ESI), enabling sub µM level detection

- Identified and quantified TCA cycle metabolites in bovine cell lysates by nano-electrospray ionization (nano-ESI), using a Thermo LC-MS equipped with orbitrap mass analyzer Outcome: Developed a highly sensitive LC MS assay with affinity chromatography enrichment that detected 5 phosphometabolites previously below the limit of detection in cell lysates

● UV-Vis spectroscopy-based protein assay & method development for albumin recovery (Jan - Dec 2017) Outcome: Developed an SOP to isolate medical grade albumin from canine plasma with 91% purity and 77% yield, supplying life saving resuscitation fluid for wounded military dogs

● Mass spectrometric (MS) signal enhancement of amino acids using MALDI-TOF MS to increase assay sensitivity for newborn amino acid screening (Aug - Dec 2016)

Outcome: Reduced matrix interference in Shimadzu Axima Resonance MALDI MS spectra of derivatized amino acids and enhanced crystallization by optimizing solvent to derivatization agent ratios GC-MS EXPERIENCE

● Method development for extraction and analysis of phenolic compounds from biomass (Jan 2021 - Dec 2024)

- Established and validated a retention index for 25 phenolic monomers and dimers that eliminated the need for mass spectrometric confirmation and reduce GC misidentifications to virtually zero

- Transferred a phenolic analysis method from a Shimadzu GC MS to an Agilent GC MS equipped with a column of different dimensions

Outcomes: - Developed an SOP for extraction and analysis of 12 therapeutic compounds from biomass

- Identified, quantified, and characterized 100+ secondary plant metabolites using untargeted and targeted approaches on both Agilent and Shimadzu GC MS platforms

- The transferred GC-MS method provided comparable separation efficiency, quantification, sensitivity, and robustness, without compromising the runtime

CE EXPERIENCE

● Developed a frontal analysis CE method to study the interactions between cyclodextrins and phenolic compounds for cost-effective sorbent selection for recovery of therapeutic phenolic compounds (Jan 2024 – April 2024) Outcome: Developed a high throughput CE method (7 minute runs, minimal sample preparation) that delivered reproducible quantitation (<3% RSD) and an alternative to HPLC testing, saving $5500+ in column costs SOFTWARE SKILLS

● Xcalibur, Chromeleon, Tunemaster (Thermo) ● LCMS, GCMS Solutions (Shimadzu) ● Empower (Waters)

● MassHunter, ChemStation (Agilent) ● 32 Karat (Beckman Coulter CE) ● Prism-GraphPad ● MS Office AWARDS

● Max Steckler Fellowship for Outstanding Oral Qualifying Exam Performance (2023), Charles H.H. Griffith Outstanding General Chemistry Teaching Assistant Award (2022), ACVECC Research Grant Award (2017) PEER-REVIEWED PUBLICATION and PRESENTATIONS

● Thomas H. Edwards, Amie Koenig, LeNae Thomas, Anjam Sadik, James L. Edwards. “Purification of Canine Albumin by Heat Denaturation in a Plasma Bag”. J. Vet. Em. Crit. Care, 2020, 30, 3, 264-271

● Sadik, A. and Bert Lynn. “Lignin-derived Value-added Chemicals for Sustainable Biorefineries”. The 75th Southeastern Regional Meeting of the American Chemical Society. Atlanta, GA 2024

● Sadik, A. and Bert Lynn. “Toward the Recovery of Value-added Lignans from Biomass”. 1st Gordon Research Symposium on Lignin. Stonehill College, MA 2024

● Sadik, A. and Bert Lynn. “Continuous Frontal Analysis Capillary Electrophoresis to Study Lignan-Cyclodextrin Interaction”. 49th Annual Naff Symposium. University of Kentucky, KY 2024

● Edwards TH, Koenig A., Thomas, L., Sadik, A. and James Edwards. “Preparation of Purified Canine Albumin by Heat Denaturation of Stored Plasma”. International Veterinary Emergency and Critical Care Symposium. Nashville, TN. Presented by co-author 2017 PROJECT MANAGEMENT EXPERIENCE

● Independently outlined a study framework and coordinated cross-lab collaboration that generated a manuscript titled “Identification and Quantification of Lignin-derived Secondary Metabolites in Ground Hurd Tissue of Cannabis sativa L. (hemp) Cultivars Aug 2024 - Present

● Spearheaded training of graduate students from 3 labs on GC MS operation and biomass treatment protocols, reducing inter-lab inconsistencies by 100 Nov – Dec 2024

● Led the training of incoming teaching assistants (TA) and revised college-level general chemistry lab experiments by streamlining procedures, enhancing safety, and improving organization, enabling efficient and safer student experience, while cutting TA training time by 60 Aug – Dec 2022 WORK AUTHORIZATION: Authorized to work immediately after graduation without employer-sponsorship (EAD in hand)

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