Molecular Biology Statistical Programming

Swetcha Radandi

814-***-**** **************@*****.*** LinkedIn GitHub BSL Certified CPR Certified EDUCATION

Gannon University, Erie, PA Jan 2024 – May 2025

Master of Science in Biomedical Engineering GPA: 3.85/4.00 Related Courses: Healthcare Systems, Nanotechnology, Surface Science, Tribology, Bio fluids Mechanics, Statistical Programming, MR fluids. Osmania University, Hyderabad, India June 2021 – May 2023 Master of Science in Genetics GPA:8.0/10.00

Related Courses: Cell Biology, Cytogenetics, Biochemistry, Biostatistics, Population Genetics, Human Genetics, Human Genomics, Microbial Genetics, Cell and Tissue Engineering, Genetic Toxicology, DNA Sequencing, Molecular Genetics, Genomics, Molecular Biology, DNA Extraction, Plant Breeding, Reverse Transcription Polymerase Chain Reaction, Next-Generation Sequencing, Sequencing, Genetics Sequencing, Parental Genetics, Pediatric Genetics, Cancer Genetics, Neurogenesis, Metabolic disorders. Osmania University, Hyderabad, India Jun 2018 – May 2021 Bachelor of Science in Genetics GPA: 9.0/10.00

Related Courses: Molecular Breeding, Mouse Models, Biostatistics, Genome Organization and Maintenance, Prokaryotic and Eukaryotic Genomics, Genome Replication, Mutation, DNA damage and Repair, Genomics, Rearrangements, Cell and Tissue Engineering. Gene Transmission in Families. SKILLS

Genetic Counseling: Patient communication, risk assessment, genetic testing and analysis, ethical considerations in genetic counseling, psychosocial assessment, patient documentation, coordination with healthcare professionals. Laboratory Techniques: DNA Sequencing, RNA Extraction, DNA Cloning, Laboratory Reagent Preparation, Bioprocessing Equipment Setup and Calibration, In-Process Monitoring, Real-Time Data Collection, GLP (Good Laboratory Practices) Compliance, Fluorescence In Situ Hybridization (FISH), Polymerase Chain Reaction (PCR), Western Blotting, Karyotyping, Gene Expression Analysis, Genetic Engineering, Recombinant DNA Technology, Cell Culture (Mammalian, Bacterial, Yeast), Bioreactor Setup and Operation, Comet Assay, Plasmid DNA Isolation, C-DNA Synthesis, Isolation of M-RNA (Trizol method), Chromatography, Enzyme Assay, Isolation of Genomic DNA from Plant/Animal/Human Tissue. Bioinformatics: Genomic Data Analysis, Computational Biology, Next-Generation Sequencing (NGS) Analysis, Bioinformatics Tools (BLAST, NCBI databases), Data Interpretation, Genetic Mapping, Disease Identification strategies, Genetic Mutations Analysis, Bioinformatics Software (R, Python, MATLAB, pandas), Linux Ubuntu. Molecular Biology: CRISPR-Cas9 Technology, Genetic Engineering, Recombinant DNA Technology, PCR (Polymerase Chain Reaction), Gene Expression Analysis, Gene Mapping, Genetic Mutations, Cell Culture (Mammalian, Bacterial, Yeast), Induction of Mutants Using Chemical Agents, RNA Extraction, C-DNA Synthesis. Statistical Analysis: R, Python, MATLAB, Excel, Cuda, Data Analysis and Process Control, Process Improvement and Optimization, Statistical Programming, Experimental Design, Data Visualization, Statistical Interpretation, Data Analytics. Genetic Counseling: Patient Communication, Understanding of Ethical Issues in Genetic Testing, Genetic Testing and Analysis, Genetic Reports and Documentation, Genetic Counseling Techniques, Risk Assessment. Clinical Genetics: Genetic Disorders, Clinical Trials, Medical Genetics, Cytogenetics, Epigenetics, Molecular Genetics, Genomics, Human Mitochondrial Genetics, Regulatory Compliance (GMP, GLP), Regulatory Filings Support. Population Genetics: Life Cycle of Drosophila, Maize Life Cycle, Segregation and Sex Determination in Maize Cobs, Scaling Bioreactor Processes (Bench to Commercial Scale), Mutant Identification and Analysis, Genetic Variation Studies. Soft Skills: Strong Communication, Team Collaboration, Problem-Solving, Critical Thinking, Time Management, Attention to Detail, Leadership, Project Management, Research-oriented, Cross-functional Collaboration, Data Presentation. EXPERIENCE

ATS GeneTech Pvt Ltd Hyderabad, India Jan 2023 – June 2023 Junior Geneticist

• Delivered genetic counseling services, explaining test results and associated risks.

• Conducted cytogenetic analyses, karyotyping, and variant interpretation for genetic disorders.

• Assisted in the recruitment and evaluation of patients for clinical genetic research.

• Managed patient registries, ensuring ethical compliance in genetic studies.

• Coordinated with multidisciplinary teams to analyze and communicate genetic findings.

• Developed patient education materials to improve understanding of genetic risks and testing procedures.

• Established robust sample collection and processing protocols for blood and tissue specimens, implementing GLP compliance and quality control measures throughout the sample lifecycle.

• Executed cytogenetic analyses including cell culture, fixation, staining, and karyotype analysis to detect chromosomal abnormalities and genetic mutations.

• Performed CRISPR-Cas9 gene editing experiments for targeted genetic research, while maintaining detailed documentation of experimental procedures and outcomes.

• Developed comprehensive data management system for sample tracking, patient information, and procedural documentation, ensuring data integrity and traceability across all research phases.

• Optimized communication channels between laboratory staff, researchers, and clinicians, coordinating sample requirements and project timelines while generating detailed cytogenetic analysis reports.

• Spearheaded genetic research initiatives by integrating genomic data analysis with clinical research designs, contributing to hypothesis testing and study outcomes.

(Biological samples, Cytogenetics analysis, Cell culture, Karyotype analysis, Genetic mutations, CRISPR-Cas9 gene editing, Genetic research, Clinical research standards, Quality control (QC), Genomic data, Data integrity, Traceability, Reports and documentation, Principal investigators, Research collaboration, Genomic results, Good Laboratory Practices (GLP), Genomic sequencing, Gene expression analysis, DNA/RNA extraction, Genetic disorders, Molecular biology techniques, Genetic testing, Biotechnology, Genetic engineering, Research methodology, Clinical trials, Bioinformatics, Molecular genetics, Precision medicine, Next-generation sequencing (NGS), PCR techniques) EXPERIENCE

Genetic Counseling Intern

Provided genetic counseling support to patients and families dealing with hereditary conditions. Assisted with genetic risk assessments and pedigree construction. Supported research efforts in genetic disorder studies, including patient data management. Educated patients on genetic conditions, inheritance patterns, and available testing options. Conducted psychosocial assessments and provided emotional support to patients undergoing genetic testing. Assisted in coordinating patient care with healthcare providers, including geneticists and primary care physicians. Siemens Hyderabad, India Aug 2022 - Jan 2023

Research Technician (Genetics Department)

• Implemented Next-Generation Sequencing (NGS) protocols for genomic sample processing and analysis, identifying genetic variations and mutations in patient samples.

• Executed CRISPR-Cas9 gene editing experiments to investigate gene functions and genetic diseases through targeted DNA sequence modifications.

• Performed essential molecular biology techniques including DNA/RNA extraction, PCR, and gel electrophoresis while maintaining detailed documentation compliant with regulatory standards.

• Perform routine molecular biology techniques such as DNA/RNA extraction, PCR, and gel electrophoresis to support genetic research.

• Optimized NGS and genetic testing protocols, collaborating with bioinformaticians to analyze genomic data and develop research hypotheses.

• Spearheaded continuous improvement initiatives by staying current with advances in genomics and CRISPR technologies, contributing strategic insights to research projects.

(Next-Generation Sequencing (NGS), CRISPR-Cas9 gene editing, Genomic samples, Genetic variations, Mutations, Sequence alterations, DNA/RNA extraction, PCR, Gel electrophoresis, Genetic research methodologies, Bioinformaticians, Genomic data, Laboratory records, Genetic assays, Genetic testing, Precision medicine, Genetic disease research, Laboratory optimization, Sequencing data) Institute of Genetics and Hospitals Hyderabad, India Sep 2019 – March 2020 Lab Intern

• Established standardized protocols for DNA isolation from human blood samples and RNA extraction from biological specimens, ensuring optimal quality for genetic analysis.

• Performed advanced genetic visualization techniques including Fluorescence In Situ Hybridization (FISH) and karyotyping to identify chromosomal abnormalities and specific gene sequences.

• Executed molecular biology procedures including PCR amplification and Western blotting for genetic mutation detection and protein function analysis.

• Conducted comprehensive pathology analysis on tissue samples to identify disease markers and genetic mutations, while maintaining detailed documentation of experimental procedures.

• Optimized laboratory operations through preparation of critical reagents for DNA sequencing and molecular techniques, while troubleshooting equipment and protocols.

• Developed process improvements in genetics and molecular biology procedures by staying current with bioinformatics advancements and implementing best practices.

( DNA sequencing, RNA extraction, DNA isolation, Human blood, Fluorescence In Situ Hybridization (FISH), Polymerase Chain Reaction (PCR), Western blotting, Pathology analysis, Karyotyping, Gene expression, Genetic mutations, Protein function, Genetic disorders, Chromosomal abnormalities, Histological analysis, Reagents preparation, Molecular biology techniques, Genetic research, Laboratory equipment maintenance, Bioinformatics)

• Metagenomic Biomarker Identification for Colorectal Cancer Diagnosis, Spring 2025

Analyzed 709 metagenomic samples from the GMrepo database to study gut microbiota differences between colorectal cancer patients and healthy controls.

Used Python and Pandas to process and mark DNA sequences for mutant base pair identification.

Built Random Forest models to identify significant microbial biomarkers for colorectal cancer diagnosis.

Conducted bioinformatics analysis in Linux/Ubuntu environments to process and visualize data.

Identified 484 microbial species and 166 genera, contributing to a deeper understanding of gut microbiota interactions in colorectal cancer.

• Applications of CRISPR/Cas9 in Gene Therapy, Spring 2025.

Reviewed the principles of CRISPR/Cas9 gene editing and its innovative modes for targeted genome modifications.

Explored the use of CRISPR/Cas9 in oncological research, including identifying cancer-related genes, modeling tumorigenesis, and studying drug resistance mechanisms.

Analyzed the application of CRISPR/Cas9 in gene therapy for treating genetic disorders, with a focus on diseases caused by point mutations.

Investigated the challenges in CRISPR-based therapies, such as delivery systems, off-target effects, and immune responses in preclinical and clinical settings.

Highlighted CRISPR/Cas9's role in advancing animal genetic models to study and treat human genetic diseases.

• Generation and Characterization of Human Induced Pluripotent Stem Cells (iPSCs) for Disease Modeling, Fall 2024

Generated human-induced pluripotent stem cells (iPSCs) from fibroblasts using non-integrating episomal vectors to ensure genetic stability.

Evaluated iPSCs for pluripotency, differentiation potential, and genetic integrity to validate their functionality.

Utilized iPSCs for modeling Parkinson’s, showcasing their utility for research & therapeutic applications.

Leveraged advancements in stem cell research to address ethical concerns associated with embryonic stem cells by using reprogrammed somatic cells.

Contributed to personalized medicine by advancing disease modeling and regenerative medicine using iPSCs.

• Class Projects, Spring 2024 - Spring 2025

Nanotechnology Fabrication of Gelatin Nanoparticles in Drug Delivery: Fabricated and studied gelatin nanoparticles for targeted and controlled drug delivery systems.

Healthcare Revenue Cycle Management: Analyzed and optimized healthcare financial processes to improve billing accuracy and revenue efficiency.

Tensile Peel Testing: Conducted tensile peel testing to evaluate adhesion strength and material properties.

Biofluids (Blood Clotting): Explored and modeled blood clotting and behavior under various conditions.

MATLAB Data Analysis: Designed MATLAB scripts for data collection, processing, and visualizations. RESEARCH

Academic Advisor Dr.Sowmya P, PhD:

PROJECTS ***Full description and code available at Linkedin/GitHub ClickHere

• The Role of chromosomal aberrations in couples with infertility

Conducted a retrospective analysis focusing on constitutional chromosomal aberrations contributing to infertility and repeated miscarriage in a cohort of 2000 couples of Indian ethnicities.

Utilized conventional G-band karyotyping analysis on phytohemagglutinin-stimulated peripheral blood lymphocytes, cultured in RPMI1640 medium, achieving 500–550 band resolution for comprehensive chromosomal enumeration.

Detected chromosomal aberrations in 110 (2.78%) couples, revealing structural chromosomal anomalies in 88 cases. Notable findings included reciprocal translocations (56 cases), Robertsonian translocations (16 cases), inversions (eight cases), deletions (three cases), derivative chromosomes (five cases), and numerical chromosome aberrations (23 cases).

Emphasized the clinical relevance of chromosomal aberrations in reproductive failure, highlighting potential genotypic and phenotypic manifestations in the progeny during gametogenesis. HONORS & AWARDS / TRAINING & CERTIFICATION

• CRISPR-Cas9 Genome Editing Certification – Completed an online program in CRISPR-Cas9 gene editing technology. - May 2019.

• Bioinformatics for Genomics Certification – Obtained certification in bioinformatics tools for genomic data analysis, 2017.

• Genetics Laboratory Techniques Training – Completed training in genetic sequencing and laboratory methods.

• Graduate Research Fellowship – Received a fellowship for innovative research in genetic disease modeling.

• RNA Sequencing and Data Analysis Certification – Gained expertise in RNA sequencing and bioinformatics analysis I hereby declare all the above information is true.

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