Research Center Ph D
Resume:
Ladan Jalali
PhD degree in molecular medicine from Urmia Iran
School of Medicine, Nazlou Campus, Urmia University of Medical Sciences, Urmia, IRAN
*******@*****.***
Education
Cellular and Molecular Research Center, Research Institute for Health Development, Kurdistan University of Medical Sciences, Iran, Sanandaj. Ph.D in Molecular medicine, 2019
Department of Physiology, Tabriz University of Medical Sciences, Tabriz, Iran M.Sc. in Physiology, 2008.
Urmia University of Medial Sciences, Urmia, Iran. B.Sc. in Nursing, 1999.
Professional Experience
Research Associate, 2013- Present
Cellular and Molecular Research Center, Research Institute for Health Development, Urmia University of Medical Sciences, Iran, Urmia
Performing cell culture
Mesenchymal stem cell isolation from umbilical cord and expansion
DNA extraction from tissues
Performing real-time PCR.
Performing electrophoresis
Proposal writing for research projects a
Teaching students about the principles of working in a biochemistry laboratory and measuring laboratory parameters with a photometric device and how to work with devices such as real-time.
Teaching students how to write research proposals
Writing review articles in most medical fields, especially in cellular and molecular fields (finding topics and designing new subtitles)
Formation and guidance of article writing groups of students and professors, especially review articles
Doing research work, in the field of medical research, data collection, data analysis and writing research papers
Perform routine laboratory upkeep such as: maintaining a high standard of safety, proper disposal of hazardous material, preparing solutions, maintaining inventory, maintaining compliance with institutional requirements, and assisting with ordering.
• Troubleshooting and optimizing the use of laboratory protocols and equipment.
• Assist with development of new technology by trying new protocols.
• Conduct analyses and report results to laboratory experiments or other operations.
• Collaborate on the update and creation of standard operating procedures.
• Conduct qualitative and quantitative research with minimal supervision to meet established deadlines for varying short-term and long-term projects
• Provides technical support for the laboratory component for courses in biochemistry
Nurse, 1999 – 2013
Shahid Motahari University Urmia, Iran
assessing and planning nursing care requirements
• providing pre- and post-operation care
• monitoring and administering medication and intravenous infusions
• taking patient samples, pulses, temperatures and blood pressures
• writing records
Working in the neonatal and emergency department as a nurse
• supervising junior staff
• organizing workloads
• providing emotional support to patients and relatives
• tutoring student nurses
Professional Skills
Skill in Human umbilical cord mesenchymal stem cell isolation and culture
Knowledge of the of MSCs isolation from bone marrow and adipose tissues
Skill in culture cells of different types of adherent and non-adherent and cell passage
Treatment of cells to precondition them, for example, with (H2O2, N-acetyl cysteine)
Staining of MSCs (with CM-DIL) to track them in the target tissues of the animal body
Stem cell therapy for animals (disease model establishment, Prepare cells for injection, MSC injection intravenously from tai vein)
Mice Models (handling, injections, dissection, euthanasia, analgesia, anesthesia, collecting of blood and tissue)
DNA and RNA extraction from tissues
Real-time polymerase chain reaction (Real-time PCR)
• Data analyzing and publications (SPSS)
Skill in writing review articles in most medical fields, especially in cellular and molecular fields (finding topics and designing new subtitles)
The ability to design studies, especially in the field of cell therapy in animals, which leads to results and writing research papers
Establish Premature ovarian failure in animal models for in vivo cell therapy candidates.
The ability to evaluate mouse and rat ovarian tissue under the microscope to identify and count the different types of ovarian follicles, and identifying healthy follicles from damaged follicles
The ability to evaluate stained ovaries with the tunnel test to check the amount of apoptosis of granulosa cells in all types of follicles.
Write and review in vivo studies protocols, schedules and coordinate with study directors
Manage, and keep animal protocols for the diseases models and study procedures for stem cell therapy.
PhD theses
effects of Preconditioned human umbilical cord Mesenchymal Stem Cells on cyclophosphamide -Induced premature ovarian failure in mice
Teaching Experience
Teaching Experience for college student in the field of physiology
Teaching Experience in the biochemistry laboratory in English
Publications
1. Jalalie, L., Rezaee, M.A., Rezaie, M.J., Jalili, A., Raoofi, A. and Rustamzade, A., 2021, Human umbilical cord mesenchymal stem cells improve morphometric and histopathologic changes of cyclophosphamide-injured ovarian follicles in mouse model of premature ovarian failure. Acta Histochemica 123, 151658. Impact factor: 2.4.
2. Jalalie, L., Rezaie, M.J., Jalili, A., Rezaee, M.A., Vahabzadeh, Z., Rahmani, M.R., Karimipoor, M. and Hakhamaneshi, M.S., 2019, Distribution of the CM-Dil-labeled human umbilical cord vein mesenchymal stem cells migrated to the cyclophosphamide-injured ovaries in C57BL/6 mice. Iranian biomedical journal.
3. Rasmi, Y., Hatamkhani, S., Naderi, R., Shokati, A., Zadeh, V.N., Hosseinzadeh, F., Farnamian, Y. and Jalali, L*, 2022a, Molecular signaling pathways, pathophysiological features in various organs, and treatment strategies in SARS-CoV2 infection. Acta Histochemica, 151908. Correspond author. Impact factor : 2.4.
4. Rasmi, Y., Shokati, A., Hassan, A., Aziz, S.G.-G., Bastani, S., Jalali, L., Moradi, F. and Alipour, S., 2022b, The role of DNA methylation in progression of neurological disorders and neurodegenerative diseases as well as the prospect of using DNA methylation inhibitors as therapeutic agents for such disorders. IBRO Neuroscience Reports. rviwe article impact factor:2.7
5. Rasmi Y, Jalali L*, Khalid S, et al. The effects of prolactin on the immune system, its relationship with the severity of COVID-19, and its potential immunomodulatory therapeutic effect. Cytokine 2023; 169: 156***-******. Corespond author. rviwe article. impact factor:3.9
6. Ladan Jalali, Azra Allahveisie, Shohreh Rezaei, Leila Ravanyar, Jafar Kiasatfar, Mohammad-Jafar Rezaie*, Amir Raoofi. Effect of Preconditioning of human umbilical cord Mesenchymal Stem Cells with hydrogen peroxide on the therapeutic potential of MSCs in the cyclophosphamide -Induced premature ovarian failure mice model. 2023. Taiwanese Journal of Obstetrics and Gynecology. original article. Impact factor: 1.94
7. Yousef. Rasmi, Ameneh Shokati, Shima. Hatamkhani, Yeganeh. Farnamian, Roya. Naderi, Ladan. Jalali1,2,*. Corespond author. Assessment of the relationship between the dopaminergic pathway and SARS-CoV-2 infection, with related neuropathological features, and potential therapeutic approaches in COVID-19 infection. reviews in medical virology. rviwe article. Impact factor: 11. Accepted. 2023
Certificates: Workshop: Primer and Probe Design for PCR at Urmia University of Medical Sciences
References
1.Mohammad Jafar Rezaie, Ph. D, Fertility and Infertility Research Center, Besat Medical Education and Treatment Center, Kurdistan University of Medical Sciences, Kurdistan, Iran. ************@*****.***
2. Yousef Rasmi, Ph. D. Department of Biochemistry, School of Medicine, Urmia University of Medical Sciences, Urmia, Iran. Email: ******@*****.***. Address: University Campus: Pardis Nazlou, 11 km of Nazlou Road, Urmia,Iran, Postal code: 571-***-****, Phone:+98-44-322*-**** Fax: +98-44-322-***-**. ******@*****.***
3. khadem Ansari. Department of Biochemistry, School of Medicine, Urmia University of Medical Sciences, Urmia, Iran. Email: *********@*****.***. Address: University Campus: Pardis Nazlou, 11 km of Nazlou Road, Urmia,Iran, Postal code: 571-***-****, Phone:+98-44-322*-**** Fax: +98-44-322-***-**.
[2]
The effects of prolactin on the immune system, its relationship with the severity of COVID-19, and its potential immunomodulatory therapeutic effect
[3]
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