Laboratory Scientist Clinical
Resume:
MEDHAT ZAKHARI
Fountain Valley, 714-***-****, ******.***@*****.***
CAREER SUMMARY
California CLS licensed experienced in laboratory operations and procedures.
Experienced in immunophenotyping Flow Cytometry staining, acquisition, and analysis for leukemia, lymphoma, minimal residual diseases and PNH.
Experienced in Chemistry and Endocrinology immunoassays.
Experienced in automated instrumentation, QC Protocols.
Experienced in writing clear SOP and easy-to-follow work instructions.
Work well under pressure and time constraints within high-volume environments.
Strong will to learn and ability to train others. EDUCATION
DEC 2017
Medical Lab. Science – Clinical Chemistry & Hematology, University of North Dakota
SEP 1998
Bachelor of Science in Veterinary Medicine, Cairo University CURRENT LICENSES AND CERTIFICATIONS
Clinical Laboratory Scientist (Generalist) – California Department of Public Health.
Medical Technologist – American Association of Bioanalysts.
(Clinical Chemistry, Hematology, Immunohematology, Immunology, and Microbiology) SKILLS
Hands-on experience in laboratory equipment set-up, operation, and preventative maintenance such as Beckman's FC 500, Navios, and BD FACS Lyric Flow cytometers, NucleoCounter NC-200, Sysmex XN-330 hematology analyzers, Siemens IMMULITE 2000, and Beckman Coulter’s AU5400 chemistry analyzers.
Administrator experience using FCS Express, APvX, LIMS, and ERP applications.
Conducted on-the-job training for new personnel.
Effectively maintains Data Integrity, GDP, and Record Keeping. Page 2 of 3
Follows test methods and laboratory procedures to successfully perform tests and report patient results.
Proactive, Flexible, Reliable, Organized, Efficient with Strong Attention to Details. PROFESSIONAL EXPERIENCE
MAY 2019 – Current NeoGenomics Laboratories, Aliso Viejo Clinical Laboratory Scientist III – Project Specialist
Process peripheral blood, bone marrow, tissue, and body fluid specimens to be stained, acquired, and analyzed for leukemia and lymphoma immunophenotyping.
Created and optimized new MRD assays on the BD FACSLyric for CLL, B-ALL, MM and most recently the AML MRD.
Proficient in new assays, equipment, reagents, and software validations.
Administrator user of FCS Express, responsible for creation, maintenance, and improvement of all templates used for flow cytometry data analysis.
Improved all analysis templates by creating new tokens to facilitate analysis and alert the analysts when, for example, a discrepancy of tube order or repeated markers was present.
Troubleshoot and perform QC tasks for laboratory instruments including Sysmex XN-330 hematology analyzers, Beckman Coulter Navios, and BD FACSLyric flow cytometers.
Created and improved QC parallel testing templates for all screening DURAClone tubes and all add-on cocktails which were also used in various validation projects.
Trained lab technicians and other scientists on how to operate, troubleshoot and maintain BD FACSLyric, and analysis of single antibody QC parallel testing.
Meets the short TAT through following FIFO rule and prioritizing rush and STAT cases. DEC 2017 – AUG 2019 Dendreon, Seal Beach
Quality Control Bioassay Analyst
Followed Test Methods and SOPs to Test & Release In-Process and Final Product samples for Critical Quality Attributes using Flow Cytometry, CBC, Viability, Gram Stain, Endotoxin level, and ELISA.
Successfully performed all QC testing using Flow Cytometry, Endotoxin, ELISA, Gram Stain, CBC, and Viability.
Effectively led by example and motivated my coworkers to “Do it Right the First time” and reduce the number of invalid assays and investigation reports.
Used my immunology and hematology background to be a Subject Matter Expert.
Cooperated with seniors and supervisors to complete required Non-Conformance investigation in a timely manner.
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MAR 2016 – DEC 2017 Dendreon, Seal Beach
Aseptic Manufacturing Operations Associate
2017 Best Performer around the whole team regarding CD54+ cells Yield% obtained after cell culture incubation.
Followed Batch Record to Aseptically manufacture PROVENGE, Personalized autologous cellular immunotherapy for metastatic castrate-resistant prostate cancer patients.
Used apheresis collected from patient to separate APCs which are then activated within cell culture pool by PAP-GM-CSF.
OCT 2014 – DEC 2015 Antech Diagnostics, Irvine
Chemistry Laboratory Technician II
Operated and released the results for more than 1500 patient samples full chemistry panel tests per shift using three Olympus Beckman Coulter AU5400 chemistry analyzers.
Maintained, adjusted, and performed more than 1000 immunoassays in more than 10 worklists using two Siemens IMMULITE 2000 chemistry analyzers.
Performed urine cortisol RIA using WIZARD Automated Gamma Counter.
Prepared and administered assays for Plasma, Serum, CSF, and Urine samples.
Practiced Good Laboratory Practices (GLP) and followed proper protocol for lab safety. RECENT TRAINING AND CEUS
Data Integrity
General Record Keeping
Decontamination Guidelines
Preparation of Technical Reports
Leading by emotional Intelligence
18 CEU – BD FACSLyric™ Operator Course
1 CEU – Vitamin D
1.5 CEU – ABO Typing Discrepancies
1 CEU – Updates in Immunotherapy
1 CEU – CV-19 & Respiratory Pathogen Testing Update
1 CEU – A Flow Cytometry View of Acute Myeloid Leukemia
2 CEU – Rh-Negative Mother with Anti-D at Delivery A Case Study
1 CEU – High Sensitivity Flow Cytometry for Paroxysmal Nocturnal Hemoglobinuria
1 CEU – Acute Myeloid Leukemia How Morphology Correlates with Immunophenotype
1.5 CEU – Autoimmune Diseases and Antinuclear Antibody Testing Methods and Staining
1 CEU – A New Collaborative Approach for Diagnosis of Lymphomas
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