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Molecular Biology.

Culture Techniques.

Microbiology.

Serology.

Immunology.

Staining

Clinical Managment.

Pharmacy Dispencing.

Managment &

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Languages

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44-8,Fourth Floor, Zakir Nagar

‘Main road, South Delhi 25

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haider4@gmailcom

ANAS HAIDER

Lab Technician/Microbiologist

Objective

To work in an environment that encourages one to face new challenges for

success and growth professionally where can utilize my skills and

knowledge appropriately.

Work Experiences

ICMR-NIOP, New Delhi

Research Trainee Uanuary 2024 - April 2021)

# Learn about Molecular Biology Techniquies for bladder cancer

Treatments

© Learn about Isolations of Genetic materials like ;RNA.DNA,Protiens from

the sampels of TissueUrine & Blood of cancer patients.

‘Adrsh Laboratory

Lab Technician (May 2021 - November 2021)

© Worked as Lab technician basically for Microbiology Lab,

Worked for Culturing of Microbiological samples like, Urine,Stool,Pus,

Sputum ete.

'® Also worked as Phlebotomist for obtaining blood samples of patients.

Felix Hospital,Noida

Lab Trainee (November 2021 - January 2022)

© Joined Felix hospital to learn the laboratory skills to enhance my self.

“Weddo Heatth pvtita,

Clinical Manager (anuary 2022 - November 2022)

# Worked as a clinical Manager for Clinical works as well as Rediology and

pharma deaprtment

“Modern Diagnosticcenter. = OOOtC~CSstsSSSS

Phlebotomist (December, 2022 - Working)

Working as a phlebotomist for sample collection as well as Routine

Experiments,

EDUCATION

Bachlers in Biotechnology, 2015-2019

MIK College,Bettiah,Bihar

Masters in Microbiology, 2019-2021

Integral University, Lucknow

Certificates

E-quizon IPR

International webinar on COVID-19 Pandamix.

National quiz on COVID-19 Pandamic

COVID-19 awareness quiz

‘Webinar on socia physiological impact of COVID-19 In academics.

Certificate of Document assitant.

Certificate of Kushal Yuva Program.

3 Days workshop on gene cloning & expression to produce GMO.

14 Days workshop on Biotechnological Instrumentations.

To detect various type of diseases urine and tissues sample have been

taken. Therefore, in our study we started our work with collection of

urine and tissue samples, then isolation of proteins from urine samples,

to check the quantity of proteins, protein quantification was done for

each sample and then their quality checked by SDS-PAGE. Western blot

was performed, samples were probed with X Antibody and rest samples

was stored at 80 c for further use. RNA and DNA was isolated from

tissues and checked at nano drop for the purity of nucleic acid in sample

then run Agarose gel electrophoresis, then RNA is converted it to cDNA

for downstream and done RT-PCR and PCR of RNA and DNA for a specific

targeted gene.

Urine and tissues sample were collected from Safdarjung hospital, New

Delhi of different types of cancer patients. Among all malignant and

non-malignant cases, malignant cases like bladder, prostate, and renal

cancer, bladder cancer was observed in more during our whole study

period. Bladder cancer is predominantly found in males as compared to

females. The percentage of male patients was more reported as

compare with women.

We found good quality and quantity of DNA/RNA from tumor tissue and

protein from urine in bladder cancer patients. The validation of GATA3

in protein sample using western and dot blot suggested that these

bimolecular may be used for downstream study of bladder cancer in

future.

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