Jean Twagirayesu Gmail: adz7kz@r.postjobfree.com

Phone: 978-***-****

Lab Skills/Techniques

· Micro pipetting

· Executing DNA extractions and purifications

· Running gel electrophoresis and SDS PAGE

· Imaging gels with a Chemi Doc Touch System

· Quantification of DNA and protein with Qubit 3.0 fluorometer & Nanodrop

· Analyzing sequencing data with bioinformatics

· Preparing working lab solutions and media

· Microsoft Excel and PowerPoint

· Purifying proteins with column chromatography

· Detecting proteins with protein immunoblots and ELISAs

· Amplification of DNA with PCR and qPCR

· Performing restriction digests to cut DNA

· Using Fragment Analyzer to determine the quantity and quality of DNA fragments

· Protein Extraction and characterization

· Protein isolation with salts

· Protein purification separating by charges

· Detecting target protein with LDH assay

· Transformation

Biotech skills

· Medical Supplies Assembly

· Packaging’s

· Boxing

· Picking

· Receiving and shipping

· General Warehouse

Education

Gloucester Biotechnology Academy - Gloucester, MA 2019

· Certificate in Biotechnology.

Gloucester High School - Gloucester, MA 2018

· High School Diploma

Work Experience

RYDER WAREHOUSE

(worked Randstad. A hiring agency )

January 2023 - may 2023.

Marking and labeling stock, and scanning.

Inspecting inventory for damage and faults.

Loading and unloading trucks.

Boxing and unboxing, tapping, retapping damage boxes.

Accepting delivery of inventory.

ELI&J LOGISTICS LLC, 1722 DIAMOND AVE NE, GRAND RAPIDS, MI, 49505

August 2021 - November 2022

Truck driver ( box truck )

Check Orders, Loading, lifting heavy stuff, palletize, loading and unloading heavy stuff into trucks and operate machinery and keep records of items loaded.

Drive and operate trucks.

Follow instructions; locate and read location and road maps.

Pre-inspect truck.

Pick up goods and materials, verify loads for accuracy, and deliver them as instructed.

Axcelis Technologies, Beverly, MA Apr 2021 – July 2022

Mechanical assembler

· Assemble electromechanical units and subassemblies to print specifications and/or manufacturing aids and be certified in the majority of the complex assemblies within a cell.

· Perform basic testing tasks within a cell to include both mechanical and electrical.

· Complete standard cell documentation forms, material requisitions, Timesheets, assembly logs, checklists, etc.

· Utilize Glovia to print shop packets, BOMs, pick lists to check inventory, and compares kit material against shop packet.

· Utilize Insight to record quality defects during assembly

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Millipore Sigma, Denver’s MA Feb 2020 - Mar 2021

Assembler

· Assembling, and packaging inside the cleanroom, also warehouse duties such as boxing, shipping, ordering, receiving, lifting heavy boxes, and picking. Was also a coordinator.

Gloucester Marine Genomics Institute Feb 2019 -May 2019

Lab technician

· Developed a protocol to isolate DNA from fungi tissues.

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