Daniel David Toy

Email: adytcy@r.postjobfree.com ** Railroad Avenue, Bear, DE 19701 Phone: 302-***-****

Education

University of Delaware Newark, DE

Bachelor of Sciences: Physics Graduated: May 2020

Minors: Mathematics, Biological Sciences

GPA: 3.017

Skills

• Proficiency with laboratory instruments

• Data Entry

• Data analysis using Excel

• Mathematical reasoning and critical thinking skills

• Apt at applying procedural technique through experimentation

• Comfortability in fundamental chemistry and biology

• Aseptic technique

• Strong working knowledge of LIMS

• Good organizational skills

Work Experience

Delaware Public Health Lab (Smyrna, DE) Aug. 2020 ~ Present

Processing Dept. Aug. 2020 ~ Feb. 2021

• Helped to streamline the processing of covid samples

• Gained extensive experience using different kinds of pipettes:

Repeater pipettes for aliquoting lysis buffer

Micropipettes and serological pipettes + pumps for pipetting patient samples

Transfer pipettes for transferring positive samples to cryovials for storage and future sequencing

Microbiology Dept. Feb. 2021 ~ Dec. 2021

• Data entry using LIMS

• Identifying various pathogens in stool, urine, throat, and wound cultures via appropriate media

• Using lab instruments such as the MALDI-TOF MS and VITEK to identify organisms and perform antibiotic susceptibility testing when appropriate

• Testing request isolates for carbapenem resistance

• Performing extractions and PCR on STEC samples

• Experience with gram staining and wet mount techniques

• Performing routine QC/QA such as:

Recording fridge/incubator temps, lot numbers and exp. dates of new media

Subculturing new media agars and broths to purity test

Routine maintenance on the MALDI and VITEK instruments

Weekly QC dilutions of our routine (control) organisms on the VITEK

Proficiency testing every 6 months

Sequencing Dept. Dec. 2021 ~ Present

• Preparing sequencing runs from In-house and external sample submitters

• Creating runs in LIMS and resulting patient samples from the cloud-based Terra biomedical platform

• In-depth experience with Kingfisher extraction protocol, Tiling and Library Prep Illumina protocols (SARS-CoV-2) for sequencing on Illumina MiSeq

• Performing routine wash maintenance on MiSeqs and NextSeqs

• Experience with operation and maintenance of Clear Labs automated nanopore sequencing technology

• Experience with CDC GHOST protocol and platform for performing HCV surveillance

• WGS on foodborne isolates

•Experience verifying and validating testing methods to demonstrate CLIA compliance standards

Academic Experience

NASA RockSat-C Program (University of Delaware) September 2019 ~ June 2020

• Coordinated with a small team to develop a Langmuir probe which would then take data on electron temperature and density in the lower layers (mostly D layer) of the ionosphere

• Became used to establishing weekly action items and meeting deadlines

• Gained familiarity with tackling problems that are open-ended/may not have a correct answer

• Built an analog subsystem to facilitate the storage of probe data into a microcontroller

• Became proficient in circuit analysis i.e. deriving voltage transfer functions to calculate expected theoretical values at different parts of a circuit

• Gained a thorough understanding of the principles of op-amps in addition to some of their applications

• Familiarized myself with technical equipment such as digital multimeters, D.C. power supplies, oscilloscopes, and function generators for the purposes of testing a circuit

• Learned KiCad for the purposes of engineering an electrical schematic

Lab TA (University of Delaware) January 2019 ~ February 2019

• Coordinated with the lab manager to give students an optimal lab experience in applications of classical mechanics

• Grading lab reports and exams according to provided rubric specifications

• Giving brief overviews of labs to students at the beginning of lab sessions

• Answering any questions students might have regarding the principles/concepts of classical mechanics

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