Ming Yin Chu Boston, Massachusetts 617-***-**** adyrza@r.postjobfree.com

EDUCATION

University of Waterloo – Bachelor of Science in Honours Biology September 2013 – April 2017

Coursework: Biology, Biochemistry, Microbiology, Molecular Genetics, Plant Molecular Biology, Population Biology, Zoology, Organic Chemistry, Biostatistics, Statistical Analysis, Embryology, Environmental Toxicology, Environmental Sciences, Economics, Calculus

WORK EXPERIENCE

CSL Seqirus (225 Wyman St, Boston, MA, USA 02451) – Research Associate I

May 2022 - Present

Member of the molecular and microbial biology team working on various pandemic influenza vaccine projects and supporting bleeding edge self-amplifying mRNA platform development

Projects focused on aspects such as enhancing stability and translation and reducing immunogenicity of vaccine candidates in preparation of clinical trials

Statistical analysis of fluorescence activated cell sorting data from in vitro expression assays

Extensive experience with cell culture using established cell lines, such as C2C12 and BHK-21 cells

RESEARCH EXPERIENCE

University of Toronto (27 King's College Cir, Toronto, ON M5S 1A1) – Walters Lab – MSc Student February 2020 – April 2022

Research focuses on the epitranscriptomic mechanisms of memory formation and fear learning

Independent researcher in a molecular neurobiology laboratory

Extensive experience and knowledge in DNA, RNA, and protein techniques and analysis

University of Waterloo (200 University Ave W, Waterloo, ON N2L 3G1) – Lolle Lab – Research Assistant September 2016 – April 2017

Composed research thesis as part of senior honours research project

Research focused on a hothead gene in A. thaliana and its expression in response to environmental stressors

Conducted research independently as well as alongside a team of laboratory members and supervisors

Presenting data and findings to research committee in a public symposium setting

PUBLICATIONS

Leonetti AM, Chu MY, Ramnaraign FO, Holm S, Walters BJ. 2020. An emerging role of m6A in memory: A case for translational priming. Int J Mol Sci. 21(20):7447.

TEACHING EXPERIENCE

BIO152 - Introduction to Evolution and Evolutionary Genetics

BIO203 - Introductory Plant Morphology and Physiology

BIO206 - Introductory Cell and Molecular Biology

BIO207 - Introductory Genetics

BIO314 - Laboratory in Cell and Molecular Biology

AWARDS

Frederick P. Ide Graduate Award in Cell and Systems Biology

TECHNICAL SKILLS

Proficient in PCR, qPCR, DNA/RNA/protein isolation, in-vitro transcription (IVT) synthesis of mRNA, Western blotting, ELISA, protein co-immunoprecipitation, microscopy, Gibson assembly, flow cytometry, and molecular cloning

Experience in handling and maintenance of cell cultures

Experience in construct design, primer design, Gibson assembly design, Golden Gate cloning design

Experience in purifying RNA and lipid nanoparticles (LNP) using tangential flow filtration (TFF)

Knowledgeable in NGS and mass spectrometry analysis, CRISPR-Cas9 gene editing, RNA biology, vaccine expression and development

Experience with statistical analysis using Graphpad Prism, Microsoft Excel, SPSS