Dr. D. Jeeva Jothi Ph.D Contact: +919********* Email:adyjpb@r.postjobfree.com

CAREER OBJECTIVE

Biomedical researcher with research experience in the field of parasitology, Angiogenesis and infectious disease. Seeking a challenging position in an intellectual stimulating environment where I can use my knowledge, expertise, skill, enthusiasm and punctuality, which will assist my career development and contribute effectively to the progress of organization. 01

BCG Vaccine Laboratory, Guindy,

Chennai, India

Technical officer February 2021 to

August 2021

02

Centre for Biotechnology, Anna

University, Chennai, India

Teaching Fellow &

Research Scholar

August 2016 to

January 2021

03 TELE DATA INFORMATIC PVT

LTD., Chennai, India

Internal subject matter

expert- E -learning

May 2009 to June

2010

04 AMET UNIVERSITY Kanathur,

Chennai, India

Visiting faculty lecturer

for B.Tech

November 2005 to

April 2009

05

New Bhavani Prince Venkateswara

College of Arts and Science,

Medavakkam, Chennai, India

Lecturer in Department

of Biotechnology

November 2006 to

April 2007

06 Annai Veilankanni Pharmacy

College, Chennai – 15

Lecturer in Department

of Biotechnology

June 2005 to

November 2006

Handled Undergraduate and Postgraduate Degree Theory and practical for: Cell Biology.

Biochemistry.

Recombinant DNA Technology.

EDUCATION

Ph.D. in

Biotechnology

Centre for

Biotechnology

Anna University,

Chennai

August 2016.

M.Phil in

Biotechnology

Department of

Biotechnology

Bharathidasan

University, Trichy

June 2008.

M.Sc in

Biotechnology

Tagore College of arts of

science

University of

Madras,Chennai

June 2005

B.Sc in Biochemistry Annai Velankanni

College

University of Madras

Chennai

June 2003

RESEARCH FELLOWSHIPS & EXPERTISE

Junior Research Fellow -Award of University Grants Commission –Basic Scientific Research

(UGC-BSR) Research fellowship on science for Meritorious StudentsCentre for Biotechnology, Anna University, from July 2011-July-2013.

Senior Research Fellow -Award of University Grants Commission –Basic Scientific Research

(UGC-BSR) Research fellowship on science for Meritorious StudentsCentre for Biotechnology, Anna University, from July 2013-July-2016.

Awarded-The American Society of Biochemistry and Molecular biology - ASBMB 2014 GRADUATE/POSTDOCTORAL TRAVEL AWARD of $1,000US.

I have been highly successful in working on assays and models, such as MTT Assay, Boyden Chamber Assay, Chick chorioallantoic member model, Tube formation assay and vasodilation assay which have coreimportance in angiogenesis.

My research focus is on angiogenesis and the establishment and elaboration of an early angiogenesis model, known as Endothelial Ring, which is the very first structural template for a blood vessel has been well standardized by me.

All molecular biology techniques can acquire and analyze samples in confocal microscopy and Flow cytometry.

PUBLICATIONS

1. Jeeva Jothi D, M Dhanraj, S Solaiappan, S Sivanesan, M Kron, A Dhanasekaran, "Brugia malayi Asparaginyl-tRNA Synthetase Stimulates Endothelial Cell Proliferation, Vasodilation and Angiogenesis ", PLOS ONE, published by PLOS ONE. Vol. 11, Issue 1, pp. 1-12 (2016). 2. Jeeva Jothi, D&AnuradhaDhanasekaran, ‘Expression and purification ofBrugia malayi Asparaginyl - tRNA Synthetase (AsnRS) mediatesAngiogenesis in Lymphatic Filariasis’,Manuscript No: EP-15- 290 .EXPERIMENTAL PARASITOLOGY

(Under review).

POSTER PRESENTATIONS

1. Jeeva Jothi D, S.Solaiyappan, M. Dhanraj, S.Chatterjee, M.Kron and Anuradha Dhanasekaran. Expression and characterization of Asparagine-tRNA synthetase: the key enzyme that mediates angiogenesis in lymphatic filariasis. Experimental Biology Annual meeting 2014, April 26-30 2014, San Diego, USA.

2. Jeeva Jothi D, Suvro Chatterjee, Anuradha Dhanasekaran.Ap3A inhibits AsnRS-induced angiogenesis in Ehy926 endothelial cells. International workshop on wound healing & angiogenesis AU-KBC Research Centre, Chennai, India, December 2013. 3. Jeeva JothiD, Anuradha Dhanasekaran ‘Expression and purification of AsparaginetRNA synthetase in lymphatic filariasis’, 23rd National congress on Parasitology, Anna University, Chennai, India 2011.

RESEARCH TITLE & ABSTRACT OF PH.D WORK

Title:RECOMBINANT BRUGIA MALAYI ASPARAGINYL- tRNA SYNTHETASE STIMULATES ANGIOGENESIS - ROLE OF CXCR2 ANTAGONIST AND Ap3A IN LYMPHATIC FILARIASIS. A hallmark of chronic infection with lymphatic filarial parasites is the development of lymphatic disease which often results in permanent vasodilation and lymphoedema, but all of the mechanisms by which filarial parasites induce pathology are not known. Prior work showed that the asparaginyl-tRNA synthetase

(AsnRS) of Brugia malayi, an etiological agent of lymphatic filariasis, acts as a physiocrine that binds specifically to CXCR2 chemokine receptors also known as interleukin -8 (IL-8). Endothelial cells are one of the many cell types that express IL-8 receptors. IL-8 also has been reported previously to induce angiogenesis and vasodilation, however, the effect of recombinant Brugia malayi asparagine-tRNA synthetase (BmAsnRS) on endothelial cells has not been reported. Therefore, we investigated the hypothesis that BmAsnRS might produce physiological changes in (EA.hy926 cells) endothelial cells. We studied the in vitro effects of BmAsnRS using a human umbilical vein cell line EA.hy926 and six different endothelial cell assays. Our results demonstrated that BmAsnRS produces consistent and statistically significant effects on endothelial cells that are identical to the effects of VEGF, vascular endothelial growth factor.

This study supports the idea that new drugs or immunotherapies that counteract the adverse effects of parasite-derived physiocrines may prevent or ameliorate the vascular pathology observed in patients with lymphatic filariasis. Neutrophil recruitment during the process of inflammation was mediated by two related receptors: CXC chemokine receptors 1 (CXCR1) and 2 (CXCR2). Interleukin-8 (IL-8) and closely related ELR (Glu-Leu-Arg) CXC chemokines are important in acute and chronicresponses to many secondary bacterial infections, wherein neutrophils are often critical to pathogen clearance. However, excessive neutrophil recruitment augments the pathology of many diseases. ELR+CXC chemokines bind CXCR1 and CXCR2 with a high affinity and have a potent chemotactic effect, particularly on neutrophils. SB225002 (N-(2-hydroxy-4-nitrophenyl)-N'-(2-bromophenyl) urea) is the first observed potent and selective non-peptide inhibitor of a chemokine receptor. It is an antagonist of 125I-IL-8 binding to CXCR2 with an IC50 = 22 nM. SB 225002 showed >150-fold selectivity over CXCR1 and four other 7-TMRs tested. This CXCR2 antagonist is identified as a useful compound to define the role of CXCR2 in inflammatory diseases where neutrophils play a major role. Depending on the number of phosphate groups, diadenosine polyphosphates (ApnA, Ap3A, Ap4A, Ap5A and Ap6A) differ in properties such as proliferation; apoptosis, vasoconstriction and vasodilatation of endothelial cells.ApnA are a family of potent intercellular and intracellular signaling molecules. Diadenosine triphosphate (Ap3A) was the first diadenosine polyphosphates to be identified in human platelets. Moreover increased Ap3A levels prevent apoptosis and thus sustain tumorgenesis. It was shown that the asparaginyl-tRNA synthetase (AsnRS) of an important human intravascular parasite, Brugia malayi, was very highly expressed and has the capacity to synthesize Ap3A in vitro conditions. Our results demonstrated that CXCR2 antagonist and Ap3A produces consistent and statistically significant effects on endothelial cells that are identical to the effects of thalidomide which acted as an anti-angiogenic factor.Our findings suggest that a filarial parasite rBmAsnRS chemo-attractant protein may contribute to the development of chronic inflammatory disease and shows pro-angiogenic effects and the CXCR2 antagonist chemokine receptors and Ap3A P2Y purine receptors acts as anti-angiogenic therapeutic targets to ameliorate parasite-induced pathology. REFERENCES

(1) Dr. ANURADHA DHANASEKARAN

Director and Head of the Department

Centre for Biotechnology

Anna University

Chennai -25

Phone: 044-********

Email: adyjpb@r.postjobfree.com, adyjpb@r.postjobfree.com

(2) Dr. SIVANESAN SUBRAMANIAN

Dean, Alagappa College of Technology,

Professor

Department of Applied Science and Technology,

Anna University,

Phone: 044-********

Email: adyjpb@r.postjobfree.com

(3) Dr. SUVRO CHATTERJEE

Associate Professor

Vascular Biology, AU-KBC Research centre

MIT campus

Anna University,

Phone: +91-44-222*****

Email: adyjpb@r.postjobfree.com, suvro@au kbc.org

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