Real Time Project Management

BinuJoy John, PhD

Missouri City, TX *****

Mobile: 713-***-****

***********@*****.***

EDUCATION & CREDENTIALS

Doctor of Philosophy in Microbiology & Immunology, 1999

University of Texas Medical Branch (UTMB), Galveston, TX

Specialization: Pathogenic Bacteriology & Molecular Immunology – Signal Transduction leading to Autoimmune Diseases

Bachelor of Science in Biology, 1992

Baylor University, Waco, TX

Minor: English

AWARDS & ACHIEVEMENTS

Space Life Sciences Directorate Special Scientific Achievement Award for the Microbiology Laboratory Team (NASA)

Space & Life Sciences Directorate Special Scientific Achievement Award for media reduction experiments through pH control (NASA)

Performance Award (MEI Technologies, Inc.)

Discovered gene for Schimke immuno-osseous dysplasia (Baylor College of Medicine)

Sigma Xi Research Award (UTMB)

Pre-doctoral Fellowship (UTMB)

SKILLS AND TRAINING

Protocol development for use in the lab, training in PCR, ddPCR (dideoxyPCR) real-time PCR, multiplex primer development, able to work with small amount of DNA samples, able to purify DNA so useful for sequencing, use of robotic liquid handling systems, mass spectroscopy analysis of DNA

Handled human and mouse blood samples, comfortable in handling various components of blood such as serum, plasma and cells, tissues and know what precautions to take for safe handling and preventing contamination

Participated in conferences and Science Symposiums, budget and oversight of finances, personnel training of summer interns and staff in modern molecular techniques in diagnosis for Microbiology

Knowledge of how to work in a BSL-2 and BSL-3 biosafety cabinets, ability to perform general laboratory skills

PROFESSIONAL EXPERIENCE

German Consulting, Sugarland, TX

HISD INTERVENTIONIST BIOLOGY TEACHER

ESL (English as a second language 9th),

PreAP (Advanced Placement 9th)

General Biology (9th to 12th)

September 6, 2022 to May 1, 2023

Help students practice reading, writing, and speaking English

Speak Spanish to help Spanish students

Help monitor students to help them with the worksheets that may be due in class or homework

Teach concepts of Biology in an easier format for the State exams, and using the computer for assignments and exams

Access Dx (Diagnostics) Laboratory, Houston, Texas

Infectious Diseases and Molecular Diagnostics

MOLECULAR BIOLOGY TECHNOLOGIST (January 12, 2022 to April 20,2022)

Performing COVID-19 testing of patient samples using established SOPs and guidance of the laboratory management

Performed RNA extraction using KingFisher equipment and setting up multiplex PCR reactions

Operate ViiA Real-time PCR equipment to test samples for presence of COVID-19 in patient samples

Analysis of Real-time PCR data using QuantStudio software and reporting results to lab supervisors in a timely manner

Maintain daily/weekly logs and QC documents for assays and equipment

Monitor inventory of supplies and equipment for long term storage

Baylor College of Medicine, Houston, Texas

Human Genome Sequencing Center

RESEARCH ASSISTANT II-GENOMIC (November 30, 2020 to May 5, 2021)

COVID-19 testing, Library construction, Manual preparation and Automated robotic preparation of samples for next generation sequencing and genotyping array assays

COVID-19 samples intake area involving patient samples for COVID-19 virus testing

LIMS (Laboratory Information Management System) for virus testing

Revised SOPs (Standard Operating Protocol) for robotic liquid sample tubes for testing at different sites

Prepared COVID-19 sample kits for testing sites

Preparation of whole genome sequencing PCR-free libraries -manual and robotic preparation

Texas Southern University, College of Pharmacy & Environmental Health Sciences, Center for Cardiovascular Diseases, Houston, TX

Research Centers for Minority Institutions in Texas

RESEARCH SCIENTIST-MOLECULAR BIOLOGY CORE

(March 10, 2019 to October 1, 2019)

Maintained the Molecular Biology Core Lab and was part of the renovation and transfer of equipment and supplies while making sure the lab equipment was functional and all supplies was remained available for all to use while also maintaining the Main Principle Investigator’s Lab area.

Mentored graduate and undergraduate students interested in Pharmacy, Environmental Health Sciences and Cardiovascular disease research.

Had financial authorization to maintain the budget such as ordering supplies and keeping track of the funding still remaining and available

Conducted ABI VIIA real-time polymerase chain reaction (PCR) experiments to study the cellular effects of PARP-1 and Clusterin gene on Butyrate treated vascular smooth muscle were started and proven.

Genitourinary Medical Oncology Research, M. D. Anderson Cancer Center (MDACC), Houston, TX

LABORATORY COORDINATOR (July 28, 2008 – January 15, 2009)

Secured research funding by converting lab manuscripts into grant applications.

Obtained further funding through NIH R0-1 grants by identifying referee comments to be addressed in revisions.

Edited and formatted PowerPoint presentations for Principal Investigator presentations held throughout the world, including Washington, Italy, and Brazil conferences (2008).

Supported development of preliminary patent/trademark applications with the US Patent and Trademark Office in conjunction with MDACC’s Office of Technology Commercialization.

Leveraged project management skills to establish timelines/milestones and achieve all deliverables.

Surface, Water & Air Biocharacterization (SWAB) Project, Microbiology Laboratory, MEI Technologies/NASA JSC, Houston, TX

SENIOR SCIENTIST (2005 to 2007)

Developed and enhanced DNA extraction methods for the SWAB project.

Devised new methods to remove inhibitors from SWAB-related samples prior to DNA extraction using the microfiltration technique.

Improved analysis capabilities by determining that re-amplification of polymerase chain reaction (PCR) products would enable low amounts of DNA in SWAB samples to be recovered, detected, and analyzed by culture-independent methods.

Enabled greater sensitivity for microbial sample analysis by incorporating use of Ruggedized Advanced Pathogen Identification Device (RAPID) instrumental for SWAB-sample analysis in real-time PCR experience.

Established Denaturing Gradient Gel Electrophoresis (DGGE) and Customer Sequencing technique to standardize analysis of mixed microbes by molecular techniques.

Incorporated uses of real-time PCR to better analyze microbiology samples and develop real-time PCR panels for opportunistic pathogens.

Ground Modular Rotating Bioreactor (gMRB) Project, Cell Science Development and Transition Section, MEI Technologies, Inc./NASA Johnson Space Center, Houston, TX

SENIOR SCIENTIST (2004 to 2005)

Optimized lactate dehydrogenase (LDH) assay and glutamine assays to monitor tissue damage and alternative energy source requirements for cell growth.

Reduced cost of performing glutamine assays by increasing assay capability five-fold; made LDH assay quantifiable.

Served as trusted agent and subject-matter expert while participating in a Weapons of Mass Destruction (WMD) exercise; provided key input on current protocols and procedures during a WMD crisis.

Effectively met all milestones and deliverables by serving as alternate cost account manager (CAM) and filling project management functions.

Optimized apoptosis assays used to understand programmed cell death in order to further monitor cell lines involved in the Critical Roadmap for Human Space Exploration.

Optimized dissociation of cells from bead matrix to further characterize cells to understand the effects of long-term space flight utilizing the simulated microgravity conditions provided by the gMRB and ARCS bioreactors.

Bioterrorism & Molecular Diagnostics Laboratory, Houston Department of Health & Human Services, Houston, TX

BIOWATCH Air Monitoring, City of Houston

LEAD MICROBIOLOGIST (2000 to 2003)

Played an integral role in the development of ricin testing using Dissociation-Enhanced Lanthanide Fluorescent Immunoassay (DELFIA).

Developed key protocols, procedures, and personnel training for the BioWatch Air Monitoring program (affiliated with Homeland Security). Was on the Select Agent list

Created new state-of-the-art techniques — including real-time PCR and time-resolved fluorometry — to detect infectious disease bacteria.

Developed and evaluated new tests and equipment — including the Roche Light Cycler, ABI 7000 Sequencer, and Victor2 1420 — to aid in bacterial detection and identification.

Department of Human & Molecular Genetics, Baylor College of Medicine, Houston, Kleberg Genotyping Center

POSTDOCTORAL FELLOW (2000 to 2003)

Mapped mouse phenotypes for the Baylor Mouse Mutagenesis Center using multiplexed SNP assays in conjunction with MALDI-TOF.

Tested and evaluated instruments and techniques — including Pyrosequencing, ABI single-base primer extension, and matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS)

Developed high-throughput mouse and human genotyping assays using SNPs (single-nucleotide polymorphisms).

Localized candidate genes for diseases by performing genome-wide linkage mapping and fine mapping.

Department of Immunology, Baylor College of Medicine, Houston, TX

POSTDOCTORAL ASSOCIATE (1999 to 2000)

Advanced cancer research by characterizing the role of PP4 (serine-threonine phosphatase), a potential tumor suppressor, using site-directed mutagenesis.

Used Western Blot Analysis to characterize the expression of proteins in different cell lines

Introduced the technique of yeast-two hybrid to the lab for understanding protein-protein interactions

GRADUATE ASSISTANT (1993 -1999)

University of Alabama – Birmingham, Department of Microbiology & Immunology, Birmingham, AL and Department of Microbiology

Completed dissertation: “Analysis of intermolecular interactions between the B cell co-receptor CD22 and stimulatory, inhibitory and regulatory effector proteins.”

Succeeded in demonstrating novel interaction of CD22 with Grb-2 and Adaptin.

Developed “Reverse Far Westerns,” a new laboratory technique to study the role of different effector protein recruitment to CD22.

PUBLICATIONS

1.Ott, C. M., Castro, V. A., Garcia, V. M., John, J., Cruz, P., Buttner, M. P., Pierson, D. L. A Comprehensive Characterization of Microorganisms and allergens in Spacecraft Environment (October 17, 2007)

2.John, B., Herrin, B. R., Raman, C., Wang, Y., Bobbitt, K. R., Brody, B. A. and Justement, L. B. 2003. The B cell coreceptor CD22 associates with AP50, a clathrin-coated pit adapter protein, via tyrosine- dependent interaction. Journal of Immunology. 170:3534-3543.

3.Boerkoel, C. F., Takashima, H., John, J., Yan, J., Stankiewicz, P., et. al. 2002. Recessive mutations of the chromatin remodeling SNF2 protein SMARCAL1 cause Schimke immuno-osseous dysplasia. Nature Genetics. 30(2): 215-220.

4.Takashima, H., Boerkoel, C. F., John, J., Saifi, G. M., et. al. 2002. Mutation of Tdp1, a topoisomerase I- dependent DNA damage repair enzyme, in spinocerebellar ataxia with axonal neuropathy. Nature Genetics. 32(10): 267-272.

5.Chen, Y. R., Kori, R., John, B. and Tan, T-H. 2001. Caspase-mediated cleavage of actin-binding and SH3-domain-containing proteins, Cortactin, HS1, and HIP-55 during apoptosis. Biochemical and Biophysical Research Communications. 288:981-989

6.Yohannan, J., Wienands, J., Coggeshall, K. M. and Justement, L. B. 1999. Analysis of tyrosine phosphorylation-dependent interactions between stimulatory effector proteins and the B cell co-receptor CD22. Journal of Biological Chemistry. 274(28): 18769-76

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