Amrit Lamichhane

***** ******** ** ******** ** **407; 832-***-****; ady0q8@r.postjobfree.com

(www.linkedin.com/pub/amrit-lamichhane/3a/a17/b05/en)

RESEARCH SKILLS/TECHNIQUES

Instrumentation: Gas Chromatography, HPLC, GC MS, GC FID, LC MS/TOF, LC MS/MS, ICP MS, Spectrometry, and PCR.

Lab Skills: Media preparation, Culture transfer, Gel Electrophoresis, Southern Blot, Western Blot, Northern Blot, Immunohistochemistry, Titration, ELISA, Affinity chromatography, Solid Phase Extraction (SPE), Wet chemistry analysis, Nomenclature, preparations, and reaction mechanisms of aliphatic and aromatic compounds.

Biotechnology: DNA Extraction, DNA Purification, Restriction Digests, Ligation Reactions, Protein Purification, DNA Sequencing, DNA Analysis, Cell culture, Primer designing, Molecular Cloning, and Site-directed Mutagenesis.

Environmental Compliance: OSHA, and NEPA

Computer: Mass hunter, ChemStation, LIMS, Blast, MS Word, MS Excel, and MS PowerPoint

EDUCATION

Master of Science, Chemistry May 2015

University of Houston- Clear Lake Houston, TX

Bachelor of Science, Biotechnology, Minor in Chemistry December2011

University of Houston- Downtown Houston, TX

PROFESSIONAL EXPERIENCE

United States Army Reserve 2021 to present

Basic Combat Training (BCT)

Advanced Individual Training (AIT)

Texas Oil Tech Laboratories, Analytical Chemist/Analyst Stafford TX 2018 to 2021

Performed extensive analysis fuel, fuel lubricants, gas, diesel and oil samples for Nitrogen, Sulfur, Metals, halides and other ions. Perform percentage moisture of soil samples.

Performed physical testing like density, viscosity, TAN, Base number, Karl fisher, Vapor pressure, Ash, Carbon residue, Asphaltene, etc. on different samples.

Xenco Laboratories, GC Chemist/Analyst Stafford TX 2015 to 2018

Performed extensive analysis of routine sample for TPH (Total Petroleum Hydrocarbon) on different matrices using TX1005 methods on GC-FID.

Perform percentage moisture of soil samples.

A and B Labs, Chemist Houston TX 2015

Performed extensive analysis of routine sample for CVAA Mercury Analyzer on different matrices (Soil, liquid, TCLP, SPLP, wipes, cassettes, particulates) using approved methods ( SW-846 7470 A, EPA 245.1, OSHAID-145)

Ran basic wet chemistry tests like Reactive Sulfide, Reactive Cyanide, Total Cyanide, total sulfide, Alkalinity, by using colorimetric, distillation and electrode method as it applies along with proper QC measures.

University of Houston- Clear Lake, Teaching Assistant, Houston TX 2013-2015

Assisted professor with Chemistry lab.

Prepared lab for EPA audit, summer 2014

QC laboratories, Lab Technician, Houston TX 2012

Measured the quality, strength and structural components of construction materials following CMET

RESEARCH PROJECTS

University of Houston- Clear Lake, Dr. Chunlong Zhang 2013-2014

Performing independent Research Project on LC MS -TOF

Conducted Non-targeted analysis of PPCPs in Wastewater Treatment Plant

University of Houston-Downtown, Dr. Philip Lyons

Performed independent Research on fungal ecology Spring 2011

Found that 11 out of 30 experimented fungi had laccase gene

ADDITIONAL ACADEMIC PROJECTS

Completed a cell biology project involving insertion of human mitochondrial signal sequence into GFP plasmid and analyzed the fluorescence of protein in mitochondria

Extracted alkaline phosphatase from the periplasmic space of Escherichia coli and quantified it