Dong-Ho Shin

***** ** **** ******* ***** Florida 33175

Mobile: 305-***-**** Email: ady0d2@r.postjobfree.com Education

****-**** **.*. **** Immunology and Molecular Biology Fukuoka Japan Kyushu University

2018-2021 MS Oriental Medicine; Acupuncture and Massage College Miami Florida 1996-1998 M.S. Fish Immunology and Molecular Biology Fukuoka Japan Kyushu University

1995-1996 Internship Fish Immunology and Histology Okinawa Japan Tropical Biosphere Research Center

University of the Ryukyus

2018-2021 BS

2018-2021 BS Health Science; Acupuncture and Massage College Miami Florida 1989-1994 B.S. Oceanography Inha University Incheon Korea Technical Skills Summary

• Excellent skills in planning, conducting, and managing projects in accordance with the laboratory regulations

• Understand and use a range of molecular genetics techniques applicable to the utilization of gene technology and the geneticengineering of micro-organisms, plants and animals

• Knowledge and techniques of recombinant protein synthesis using prokaryotic and cell-free systems

• Experience and techniques of preparation of polyclonal antibodies using rabbits

• Clone and express foreign genes in prokaryotic system

• Perform nucleic acid isolation and manipulation, gene cloning and PCR amplification, DNA sequencing and computer analysis of gene sequences, immunological detection of proteins, and the genetic transformation and assay of mammals, fish and plants

• Detect the. presence of biological agents that have been intentionally released into the air

• Excellent skills with handling of human blood-borne pathogenic samples (HIV and HCV), detection and analyzing skills of SNP of Diabetes marker genes and evaluating oxidative stress in HIV and HCV subjects

• Use a core set of programs and databases for nucleotide and amino acid sequence analysis and phylogenetic reconstruction

• Excellent understanding of molecular biology and comparative immunology Employment Experience

Oct 2019 - Present Biological/Laboratory Scientist, A-TEK Miami Florida Aug 2017 - May 2018 Life Science Teacher (Voluntary), Revolution School Doral Florida May 2016 - Sep 2016 Research Professor,Wonkwang University South Korea

• Conducting and developing early diagnostic system on the West Nile virus infection in BSL2 laboratory

• Managing and organizing interdisciplinary convergence research workshop

• Supervising graduate students and writing grant proposals and scientific manuscripts Apr 2014 - May 2016 Senior Research Scientist, Nat'l Academy of Agricultural Sci. South Korea

•Conducting and managing research projects on the bellflower genome in BSL1 laboratory

• Supervising and training 3 researchers

• Preparing scientific manuscripts for publication Dec 2012 - Aug 2013 R&D Manager,Seaever Co. Ltd. South Korea

• Developing triploid Pacific oyster

• Supervise 5 researchers in BSL1 laboratory

• Managing research contract with the Republic of Colombia Apr 2009 – Dec 2012 Research Assistant Professor Dept. Dietetics & Nutrition, Fla Intl' Univ. Miami Florida

• Developing and initiating new clinically eligible measuring systems in BSL2 laboratory

• Assisting and training undergraduate and graduate students, including overseeing daily progress of students

• Managing and supervising daily operation of research projects (e.g., real-time PCR and HIV/HCV infection) from data production to preparation of manuscripts

• Teaching a designated class

Sep2001 - Mar 2009 Postdoctoral Fellow, Dept. Biological SciencesFla Intl' Univ. Miami Florida

• Overseeing daily progress of students

• Assisting and training undergraduate and graduate students (e.g., conventional PCR)

• Managing and supervising research projects and writing grant proposals and scientific manuscripts

• Teaching two designated workshops

Academic Activities

2010-2012 Mentoring one Doctoral student and two Master students, Florida International University Apr 2008 Reviewer for student summer research grants, Florida International University May 2008 Visiting researcher(Department of Biochemistry), University of Oxford 2003-2007 Ad hoc committee member of two master students' thesis and mentoring Florida International University graduate and undergraduate students

Selected publications and manuscripts

1) Shin, D-H., etal. (2016) The complete chloroplast genome of the Hare’s Ear Root,Bupleurumfalcatum: its molecular implications.Genes 7, 20.

2) Shin, D-H.,etal. (2012) Relationship of oxidative stress to HIV diseaseprogression in HIV/HCV co-infection compared to HIV mono-infected adults in Miami. Int’l. J. Biosci. Biochem.Bioinform. 2:217-222. 3) Graham, M., Shin, D-H, and Smith, SL. (2009) Molecular and expression analysis of complement C5 in the nurse shark

(Ginglymostomacirratum) and its predicted functional role. Fish & Shellfish Immunol.27: 40-49 4) Shin, D-H., etal. (2007) Molecular cloning, structural analysis and expression of complementcomponent Bf/C2 genes in the nurse shark, Ginglymostomacirratum. Dev Comp Immunol 31: 1168-1182. 5) Shin, D-H., etal. (2001) Organization of the NKEF gene and its expression in the common carp(Cyprinus carpio). Dev. Comp. Immunol. 25: 597-606.

6) Fujiki, K., Shin, D-H., Nakao, M, and Yano, T. (1997) Protective effects of k-carrageenan against Bacterial insfections in carp Cyprinus carpio. J.Fac. Agr. Kyushu Univ. 42(1.2): 113-119. 7) Fujiki, K., Shin, D-H., Nakao, M, and Yano, T. (1997) Effects of k-carrageenan on the non-specific defense system of carp Cyprinus carpio. Fisheries science. 63(6): 934-938. References

Manuel A Ramirez. Lead Field Service Technician

Address: Thermo Fisher Scientific,

5971 Van Allen Way

Carlsbad. CA 92008, USA

Email: ady0d2@r.postjobfree.com

Phone: 1-786-***-**** or 1-800-6288 (Opt 3)

Aracelis Escevarria, Biological Scientist,

Address: 1325 NW 14th Avenue Miami, FL 33125

Phone: 787-***-****

Email: ady0d2@r.postjobfree.com

Dmitre St. Surin, Laboratory Scientist,

Address: 1325 NW 14th Avenue Miami, FL 33125

Phone: 954-***-****

Email: ady0d2@r.postjobfree.com

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