Daun Everforest, Ph.D.

Senior Technical Application Scientist

Determined and well-rounded Technical Scientist with more than 15 years experience within Biotech industry as well as academia. Sharp and focused professional offering skills in synthetic biology, molecular DNA and recombinant biology, NGS, immunoassay development, RNAi, CRISPR, ELISA, FACS, fluorescent in-vivo imaging, PK-PD, Immunogenicity, micro-fluidics and next-gen sequencing. Proven ability in development of novel applications and experimental design. Expertise in Salesforce, Excel, Powerpoint, SAS

Skills

Assay development

Quantitative analysis

Training and mentoring

Research implementation

Research and experiments

Experimental Design

Presentation management

Method validation

NGS Long read sequencing

Project Management

Results analysis

Report generation

CRM tools

Molecular Biology

SynBioTechnical knowledge

GLP/GMP compliance

Work History

2022-01 - 2023-06 Senior Technical Applications Scientist Telesis Bio, San Diego, CA

San Marcos, CA 92078

858-***-****

adxybj@r.postjobfree.com

Develop and execute laboratory work plans and schedules for self and team, using project development milestones and division performance. Interact with internal staff and management to identify assays requiring further improvements or development and oversees improvement of these assays.

Supervise technical staff, including work assignment, performance and development management.

Assist in development of strategies for process improvements. Assist in training junior level staff with goal of guiding/mentoring all members of development team.

Interpret, evaluate, communicate and properly document results of development work and make recommendations for action. Helped transform customer experience by implementing new support tools and processes.

Developed conversion and system implementation plans. Sought and developed process improvements to provide service and support, meet revenue targets and improve customer satisfaction. Developed, wrote and edited technical documentation and training materials for internal use.

Processed, analyzed and interpreted large-scale genomic and proteomic datasets.

Analyzed and interpreted data from genomic and proteomic experiments to identify potential biomarkers.

Provided continuous feedback to product groups on system performance, features and potential problems while collaborating with development groups on future product developments.

Reported on actual performance relative to goals and provided variance analysis and revised projections.

2019-02 - 2022-01 Senior Lead Scientist

Proper Cell Bio, San Diego, CA

Simultaneously managed several complex projects, meeting all pertinent milestones.

Trained 10 new employees on areas of technical expertise and compliance issues relevant to lab setting.

Applied theories and methods of life sciences to interpret and perform analyses of experimental results.

Conducted document review, organization and quality control of data acquisition to draw relevant conclusions and direct research activities. Advised or assisted in obtaining patents or meeting other legal requirements.

Evaluated and implemented new technologies and laboratory techniques to improve quality and robustness of sample analysis. Conducted experiments in laboratory environment for product development purposes.

Designed laboratory and automation workplace. GLP/ GMP compliance 2013-08 - 2022-01 Faculty Instructor

California State University, San Marcos

Molecular Cell Biology Lecture and Lab, Microbiology Lecture and Lab, Biotechnology Lecture and Lab, Biotechnology Professional Masters Program.

Design and development of molecular biology laboratory courses for graduate and undergraduate University students.

Develop current protocols and presentations designed for mastery of molecular biology lab.

Lab curriculum and assay preparation including CRISPR-Cas modification in S.

Pombe, Recombinant protein expression and purification, ELISA, Western blot analysis, FACS, DNA cloning, qPCR, Gibson cloning, RNA/DNA extraction,.

Phylogeography of Critically Imperiled Grand Canyon Masked Clubskimmer (Brechmorhoga pertinax).

2002-07 - 2021-06 Graduate Research Scientist

San Diego State University Research, San Diego, CA Dr Skaidrite Krisans Dissertation Project: Identification and Characterization of Novel Isopentenyl Diphosphate Isomerase in Humans. Expression and purification of novel protein in bacteria and yeast systems. Development of in-vitro cell based and enzymatic assays. Testing and identifying enzyme kinetics in vitro.

Complementation testing in vivo in S. cerevisiae.

Protein expression in cultured cells.

Immunofluorescence and analysis of expressed proteins by confocal microscopy.

Prepared annual NIH Grant updates, prepared and submitted AHA Grants. Wrote research papers, reports, reviews and summaries regarding isoprenoid biosynthesis.

2013-03 - 2015-08 Research Scientist Molecular Genetics San Diego Zoo Institute for Conservation Research

DNA bar-coding.

Genomics.

Biostatistics.

Genetic analysis.

Population and genome analysis studies.

2009-08 - 2012-10 Field Application Scientist

LI-COR Biosciences

Technical Applications Scientist with extensive assay development skills including immunoassay, RNAi, ELISA, in vivo fluorescent imaging. Prepare and deliver technical presentations to scientific audiences. Help maintain and increase product usage, and support sales efforts through technical presentations and demonstrations. Provide pre-sale and post-sale support including applications, experimental design, reagent support.

Provide front-line troubleshooting of issues related to user's technical skills, biology/ assay design and experimental design.

Develop and maintain positive relationships with customers in assigned territory.

Work with Sales Consultants/Product Managers on commercial initiatives aimed at defining customers' needs for new and improved products and services.

Work with development teams to identify and help characterize new applications in Western detection and in-vivo imaging and relate these applications to capabilities and products to allow the capture of new markets.

Help specify and develop technical support materials and user manuals for new and current products.

Work with the Marketing team to contribute to journal articles, applications notes and other marketing and technical collateral to support products at seminars, scientific meetings and trade shows.

Work with other team members to develop sales and field applications technical training programs and participate in their execution. Act as a key contributor of customer feedback to Marketing and Product Development teams.

Develop and improve key company processes that support building a world-class customer support organization.

2006-09 - 2010-01 Adjunct Faculty

California State University, San Marcos

Courses taught:.

Molecular Cell Biology, lecture and lab.

RNA and Genomics Technologies for Masters.

Conservation Biology.

Human Nutrition.

Human Reproduction.

Cancer Biology.

Masters Molecular Biology Seminar.

Course design for Biostatistics for Nurses.

2007-09 - 2009-08 Field Application Scientist

Gyros, Inc

West Coast Representative for Applications for novel detection of immunoassays, ADA, ATA.

Assay development including biomarker identification, PK/PD assay, Immunogenicity.

Immunoassay development for new and existing customer accounts. In-depth customer training and individualized assay design. Coordinate and lead presentations for potential West coast customers. Organized conference poster presentations/demonstrations. Assist in technical/software applications and data analysis. CRM management.

2006-05 - 2007-09 Post-doctoral Researcher

California State University, San Marcos

Laboratory supervisor for team of graduate and undergraduate students. Validation of high-density oligonucleotide microarray analysis using QPCR. Coordinator and member of Annotation Team for genome annotation the model coccolithophorid Emiliania Huxleyi, focusing on genes involved in dimethylsulfide emissions in E.

Huxleyi,.

Development of genetic transformation system for marine coccolitophorids using biolistics and cationic lipid based technologies. Recombinant vector design for expression and detection of in-vitro transcribed RNAs in coccolithophorids.

Novel protein expression and purification in S.

Cerevisea.

1997-09 - 2001-01 Graduate Research Assistant

San Diego State University

Project: Investigation of the Transcription/Replication Regulatory Mechanisms in the Vesicular Stomatitis Virus (VSV) L protein. Site-directed mutagenesis of VSV L protein created mutation within a region identified as playing a role in regulation of replication and transcription in VSV.

Molecular cloning and recombinant VSV L protein expression and purification.

Transcriptional enzyme assay design, development and implementation. 1996-08 - 1997-06 Research Assistant

California State University, San Marcos

Use of Microsatellite Markers in the Hybridization Rate of two closely related species of Bluegill Sunfish (Lepomis Macrochirus). Genetic diversity analysis of captive populations of penguins using molecular genetic techniques.

1995-05 - 1996-06 Research Fellow

Center For Reproduction, Endangered Species, Molecular Genetics Use of Enrichment Technique for Creation of Microsatellite Library from Genomic Black Rhino DNA.

Project involved use of non-invasive techniques for isolation of genomic DNA from endangered population of captive Black Rhino. Construction of genomic library enriched for microsatellite sequences. Evaluation of microsatellite sequences as individual specific loci for the purpose of unambiguous identification of individuals as well as degrees of relatedness within a captive population of endangered black rhino. Education

1997-06 - 2006-05 Ph.D.: Cell and Molecular Biology University of California San Diego, San Diego State University Joint Doctoral Program Dissertation title: Characterization of a Novel Isozyme of Isopentenyl Diphosphate Isomerase in Humans Advisor: Dr Skaidrite Krisans Received ARCS Scholarship 1997-2006

Dean's List 1997-2006

1997-05 Bachelor of Science: Biological Science

California State University - San Marcos

GPA: 4.0/4.0, Cum laude

Accomplishments

Supervised team of 8-10 staff members.

Achieved platform launch by completing testing and implementation with accuracy and efficiency.

Documented and resolved multiple product issues which led to customer project design resolution.

Affiliations

American Society of Virology (ASV) American Association for Advancement of Science (AAAS)

Additional Information

HONORS AND AWARDS, ARCS Foundation Scholar 1999-2005 Cum Laude, CSUSM 1997 NSF-CRES, San Diego Zoo Fellowship 1995-1996 Kellogg Undergraduate Scholarship 1993-1995

Awards

ARCs Scholar UCSD 1997-2006

Outstanding Lecturer Award, College of Biological Science, California State, University San Marcos 2019

President's Award for Outstanding Lecturer, California State, University San Marcos 2021-22

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