Service Representative Research Assistant

Michael J Osborne Curriculum Vitae *

Michael J

Osborne, PhD

Biochemistry Research Assistant

Biophysics and NMR platform

Manager

P: 514-***-****

E: *******.*******@*********.**

A: 919 Avenue De L’Epee, Montreal, Qc, H2V 3V2, Canada ORCID: https://orcid.org/0000-0003-1028-4038

Nationality: British, Canadian Permanent Resident

Research Interests

• Characterising clinically relevant protein:protein and protein:small molecule interactions via biophysical techniques

(NMR, ITC, CD, AUC, fluorescence)

• Structure and dynamics of proteins and their complexes using NMR

• Identifying small molecule as potential therapeutics using NMR-based fragment screening

• SAR using NMR techniques for ranking of binders

• Manager/maintenance of NMR spectrometers (800, 600, 400 Varian and Bruker with Cryoprobes) and biophysics instruments (ITC, CD AUC, Fluorescence)

Research Experience

Research Associate

Prof. Katherine Borden

2008 – Present

Biophysics and NMR Platform

Manager

2008-present

Institute for Research in Immunology and Cancer (IRIC); University of Montreal

• Investigated multiple protein/protein interactions important in cancer (typically involving eIF4E), protein/small molecule interactions using biophysical methods including NMR (chemical shift perturbation, transferred cross-saturation, HADDOCK-derived models), ITC, CD, fluorescence and AUC.

• NMR protein structure determination of multiple proteins; including with small molecules

• Initiated NMR fragment screening at IRIC, including collation of in-house compounds to generate a small (400 compound) library after QC using CMCq and CMCa (BRUKER) packages. Currently overseeing 3000 compounds

• Mentored graduate and post graduate students

Institute for Research in Immunology and Cancer (IRIC); University of Montreal

• Maintenance, operation and troubleshooting of Bruker (600/400 MHz) and Varian/Agilent (600/400 MHz) NMR spectrometers/magnets including cryoprobes.

• Liquid helium and liquid nitrogen cryogen magnet fills

• Routine acquisition of 2D, 3D, and 4D NMR experiments to characterize proteins; including assignment, structure determination and dynamics

• Implemented 1D and 2D automation routines for chemists and biochemists on Bruker and Varian spectrometers using carousel and samplejet sample handlers

• Automated Q.C. (including compound integrity and solubility determination in aqueous buffers) of compound libraires using CMQC/CMCA and ICONMR (ran for Maybridge and Key Organic fragment libraries 3000 compounds)

• NMR training of diverse set of scientists (medicinal chemists, molecular biologists etc) for acquisition and analysis of a wide range of NMR experiments Michael J Osborne Curriculum Vitae 2

Research Assistant

2003-2007 (Dr. Kalle Gehring)

Research Assistant

1998-2003 (Prof. Feng Ni)

Post-Doc

1995-1998 (Dr. P.E. Wright)

PhD Dissertation/Post-Doc

1989-1995

Advisor Prof G.R.Moore

• Manager of biophysics instruments AUC, ITC, CD and fluorimeter at IRIC: responsible for maintenance and training

McGill University

• Structural genomics, with an emphasis to increase throughput of three dimensional structures from NMR (NUS techniques)

• Use of alignment techniques to improve NMR structures and NMR relaxation techniques for identifying domain motions in proteins.

• Oversaw installation and site planning (new building) for a 800 MHz Varian system

Biotechnology Research Institute

• Investigating determinants of affinity and specificity in Cdc42-effector protein complexes, focusing on Cdc42 interactions with effector proteins essential for virulence in Candida albicans (Cla4 and Ste20).

• Monitoring binding hotspots in tight protein-protein complexes using relaxation experiments as a rapid alternative to structure determination.

• Expression of peptides in E. coli

Scripps Research Institute

• Functional dynamics of DHFR were unravelled using 15N relaxation NMR experiments

• Developed novel methods to identify and distinguish the effects of rotational diffusion anisotropy on Modelfree analysis in DHFR complexes (COPED)

• Assigned backbone chemical shifts for DHFR and NCAM proteins University of East Anglia. Thesis title: A Study of Interacting Species by NMR

• Structure determination of the immunity protein of Colicin E9, Im9 - the first example of a structure in this class.

• Structure determination of the paramagnetic protein, ferredoxin I, from Desulfovibrio africanus.

• Development of methods to determine the cluster geometry of the 4Fe-4S centres.

Skills

• Acquisition and processing of solution state NMR experiments for protein and small molecule samples including; triple resonance (up to 4D), relaxation, select filter-edit NOESY, diffusion and residual dipolar coupling experiments. Frequently use NUS schedules for multidimensional experiments

• Running and organisation of multiple biophysics instruments as part of a core facility for multiple users

• Extensive experience with NMR softwares for processing and analysis including (but not limited to) nmrDraw, NMRViewJ, TOPSPIN for NMR data processing and CYANA/XPLOR for structure calculations. Experience with MREstnova

• Analysis of NMR data for binding: including linehsape analysis (TITAN, LineShapeKin) and PCA analyses (TRENDNMR)

• Experience in relaxation analysis, Modelfree, etc: developed a novel method (COPED) for anisotropy in Modelfree analyses.

• Basic coding and implementation of newly published NMR sequences for Aglient/Varian and Bruker systems

• Protein and peptide expression and isotopic enrichment with 15N/13C/2H.

• Protein and peptide purification, including extensive experience with FPLC, HPLC, SDS-PAGE techniques.

• Characterisation of protein complexes using various methods including NMR, CD, AUC, ITC

• Background in Unix and Linux operating systems NAWK, C-shell programming skills

• Knowledge of KNIME and basic chemoinformatics

• Ability to work in both autonomous and group environments.

• Initiated Fragment screening using NMR (including library generation, pooling strategies and analysis) at IRIC

• Articulate and organised, with clear presentation abilities

• Contributed heavily in writing successful grant applications and peer reviewed papers Michael J Osborne Curriculum Vitae 3

Teaching and supervisory experience

• Supervision of technicians, undergraduate and graduate students during my time at IRIC, often directing research projects which were NMR related

• Teaching introductory NMR courses to post-graduates at University of Montreal; 2 hour course on application of NMR in biology

• Teaching basic setup and processing of multidimensional NMR experiments for proteins and small molecules to researchers at University of Montreal

Presentations

Many internal presentations at IRIC/UdeM not included here.

“Development of NMR fragment screening at IRIC as a platform. Application to an enzyme involved in a novel resistance mechanism”. 7th Scientific day, Institute for Research in Immunology and Cancer, University of Montreal, 26th May 2017 .

"Dynamics and Catalytic Function of DHFR: Insights from NMR" American Chemical Society National Meeting in Orlando, Florida, 7th-11th April, 2002 in the Physical Chemistry Division

("Modern Aspects of Structure Function Correlation of Biomolecules: Enzyme Action") organised by Amnon Kohen.

"Effects of rotational diffusion anisotropy on the model-free analysis of protein dynamics in the solution state: application to complexes involving dihydrofolate reductase”. MOOT NMR symposium, McGill University, Montreal, Canada, 25th September 1999.

"Neglect of rotational diffusion anisotropy in the model-free analysis of dihydrofolate reductase leads to erroneous millisecond motions for over half of the analysed spins". Biotechnology Research Institute, National Research Council of Canada, Montreal, 2nd June 1998

"Anisotropic model-free analyses of motions of complexes in the catalytic cycle of dihydrofolate reductase: evidence for a functional role of motions". Norwich Research Park, University of East Anglia, Norwich, UK, 10th May, 1998.

"The role of NMR in rational drug design". University of East Anglia, Norwich, UK, 12th May, 1998

"NMR techniques for characterising protein:protein interactions - applications to the cytochrome c: cytochrome b5 and Colicin E9: immunity protein systems".University of California San Diego, La Jolla, 12th April, 1995 and University of Leiden, 4th, June, 1995.

Publications

1: Osborne MJ, Volpon L, Memarpoor-Yazdi M, Pillay S, Thambipillai A, Czarnota S, Culjkovic-Kraljacic B, Trahan C, Oeffinger M, Cowling VH, Borden KLB. Identification and Characterization of the Interaction Between the Methyl-7-Guanosine Cap Maturation Enzyme RNMT and the Cap-Binding Protein eIF4E. J Mol Biol. 2022 Mar 15;434(5):167451. doi: 10.1016/j.jmb.2022.167451. Epub 2022 Jan 10. PMID: 35026230. 2: Osborne MJ, Rahardjo AK, Volpon L, Borden KLB. 1H, 13C and 15N chemical shift assignments of the C-terminal domain of human UDP-Glucuronosyltransferase 2B7 (UGT2B7-C). Biomol NMR Assign. 2021 Oct;15(2):323-328. doi: 10.1007/s12104- 021-10024-9. Epub 2021 Apr 18. PMID: 33870481; PMCID: PMC8549657. 3: Coutinho de Oliveira L, Volpon L, Rahardjo AK, Osborne MJ, Culjkovic- Kraljacic B, Trahan C, Oeffinger M, Kwok BH, Borden KLB. Structural studies of the eIF4E-VPg complex reveal a direct competition for capped RNA: Implications for translation. Michael J Osborne Curriculum Vitae 4

Proc Natl Acad Sci U S A. 2019 Nov 26;116(48):240**-*****. doi: 10.1073/pnas.190*******. Epub 2019 Nov 11. PMID: 31712417; PMCID: PMC6883836.

4: Coutinho de Oliveira L, Volpon L, Osborne MJ, Borden KLB. Chemical shift assignment of the viral protein genome-linked

(VPg) from potato virus Y. Biomol NMR Assign. 2019 Apr;13(1):9-13. doi: 10.1007/s12104-018-9842-3. Epub 2018 Sep 21. PMID: 30242622; PMCID: PMC6428624.

5: Osborne MJ, Coutinho de Oliveira L, Volpon L, Zahreddine HA, Borden KLB. Overcoming Drug Resistance through the Development of Selective Inhibitors of UDP-Glucuronosyltransferase Enzymes. J Mol Biol. 2019 Jan 18;431(2):258-272. doi: 10.1016/j.jmb.2018.11.007. Epub 2018 Nov 11. PMID: 30428301; PMCID: PMC6331242. 6: Volpon L, Osborne MJ, Borden KLB. Biochemical and Structural Insights into the Eukaryotic Translation Initiation Factor eIF4E. Curr Protein Pept Sci. 2019;20(6):525-535. doi: 10.2174/1389203720666190110142438. PMID: 30636602. 7: Osborne MJ, Coutinho de Oliveira L, Volpon L, Borden KLB. Backbone assignment of the apo-form of the human C- terminal domain of UDP-glucuronosyltransferase 1A (UGT1A). Biomol NMR Assign. 2018 Oct;12(2):315-318. doi: 10.1007/s12104-018-9830-7. Epub 2018 Jun 22. PMID: 29934866; PMCID: PMC6309593. 8: Volpon L, Culjkovic-Kraljacic B, Sohn HS, Blanchet-Cohen A, Osborne MJ, Borden KLB. A biochemical framework for eIF4E- dependent mRNA export and nuclear recycling of the export machinery. RNA. 2017 Jun;23(6):927-937. doi: 10.1261/rna.060137.116. Epub 2017 Mar 21. PMID: 28325843; PMCID: PMC5435865. 9: Volpon L, Culjkovic-Kraljacic B, Osborne MJ, Ramteke A, Sun Q, Niesman A, Chook YM, Borden KL. Importin 8 mediates m7G cap-sensitive nuclear import of the eukaryotic translation initiation factor eIF4E. Proc Natl Acad Sci U S A. 2016 May 10;113(19):5263-8. doi: 10.1073/pnas.152*******. Epub 2016 Apr 25. PMID: 27114554; PMCID: PMC4868427. 10: Osborne MJ, Borden KL. The eukaryotic translation initiation factor eIF4E in the nucleus: taking the road less traveled. Immunol Rev. 2015 Jan;263(1):210-23. doi: 10.1111/imr.12240. PMID: 25510279; PMCID: PMC4269245. 11: Peng H, Talebzadeh-Farrooji M, Osborne MJ, Prokop JW, McDonald PC, Karar J, Hou Z, He M, Kebebew E, Orntoft T, Herlyn M, Caton AJ, Fredericks W, Malkowicz B, Paterno CS, Carolin AS, Speicher DW, Skordalakes E, Huang Q, Dedhar S, Borden KLB, Rauscher FJ 3rd. LIMD2 is a small LIM-only protein overexpressed in metastatic lesions that regulates cell motility and tumor progression by directly binding to and activating the integrin-linked kinase. Cancer Res. 2014 Mar 1;74(5):1390-1403. doi: 10.1158/0008-5472.CAN-13-1275. PMID: 24590809; PMCID: PMC4183205. 12: Volpon L, Osborne MJ, Zahreddine H, Romeo AA, Borden KL. Conformational changes induced in the eukaryotic translation initiation factor eIF4E by a clinically relevant inhibitor, ribavirin triphosphate. Biochem Biophys Res Commun. 2013 May 10;434(3):614-9. doi: 10.1016/j.bbrc.2013.03.125. Epub 2013 Apr 10. PMID: 23583375; PMCID: PMC3659399. 13: Volpon L, Osborne MJ, Culjkovic-Kraljacic B, Borden KL. eIF4E3, a new actor in mRNA metabolism and tumor suppression. Cell Cycle. 2013 Apr 15;12(8):1159-60. doi: 10.4161/cc.24566. Epub 2013 Apr 9. PMID: 23587918; PMCID: PMC3674077.

14: Osborne MJ, Volpon L, Kornblatt JA, Culjkovic-Kraljacic B, Baguet A, Borden KL. eIF4E3 acts as a tumor suppressor by utilizing an atypical mode of methyl-7-guanosine cap recognition. Proc Natl Acad Sci U S A. 2013 Mar 5;110(10):3877-82. doi: 10.1073/pnas.121*******. Epub 2013 Feb 19. PMID: 23431134; PMCID: PMC3593863. 15: Fontan L, Yang C, Kabaleeswaran V, Volpon L, Osborne MJ, Beltran E, Garcia M, Cerchietti L, Shaknovich R, Yang SN, Fang F, Gascoyne RD, Martinez-Climent JA, Glickman JF, Borden K, Wu H, Melnick A. MALT1 small molecule inhibitors specifically suppress ABC-DLBCL in vitro and in vivo. Cancer Cell. 2012 Dec 11;22(6):812-24. doi: 10.1016/j.ccr.2012.11.003. PMID: 23238016; PMCID: PMC3984478.

Michael J Osborne Curriculum Vitae 5

16: Siddiqui N, Tempel W, Nedyalkova L, Volpon L, Wernimont AK, Osborne MJ, Park HW, Borden KL. Structural insights into the allosteric effects of 4EBP1 on the eukaryotic translation initiation factor eIF4E. J Mol Biol. 2012 Feb 3;415(5):781-92. doi: 10.1016/j.jmb.2011.12.002. Epub 2011 Dec 9. PMID: 22178476; PMCID: PMC3269305. 17: Volpon L, Osborne MJ, Capul AA, de la Torre JC, Borden KL. Structural characterization of the Z RING-eIF4E complex reveals a distinct mode of control for eIF4E. Proc Natl Acad Sci U S A. 2010 Mar 23;107(12):5441-6. doi: 10.1073/pnas.090*******. Epub 2010 Mar 8. PMID: 20212144; PMCID: PMC2851782. 18: Rolef Ben-Shahar T, Castillo AG, Osborne MJ, Borden KL, Kornblatt J, Verreault A. Two fundamentally distinct PCNA interaction peptides contribute to chromatin assembly factor 1 function. Mol Cell Biol. 2009 Dec;29(24):6353-65. doi: 10.1128/MCB.01051-09. Epub 2009 Oct 12. PMID: 19822659; PMCID: PMC2786881. 19: Volpon L, Osborne MJ, Borden KL. NMR assignment of the arenaviral protein Z from Lassa fever virus. Biomol NMR Assign. 2008 Jun;2(1):81-4. doi: 10.1007/s12104-008-9090-z. PMID: 18958179; PMCID: PMC2574003. 20: Gutiérrez P, Kozlov G, Gabrielli L, Elias D, Osborne MJ, Gallouzi IE, Gehring K. Solution structure of YaeO, a Rho-specific inhibitor of transcription termination. J Biol Chem. 2007 Aug 10;282(32):23348-53. doi: 10.1074/jbc.M702010200. Epub 2007 Jun 11. PMID: 17565995.

21: Peng H, Gibson LC, Capili AD, Borden KL, Osborne MJ, Harper SL, Speicher DW, Zhao K, Marmorstein R, Rock TA, Rauscher FJ 3rd. The structurally disordered KRAB repression domain is incorporated into a protease resistant core upon binding to KAP-1-RBCC domain. J Mol Biol. 2007 Jul 6;370(2):269-89. doi: 10.1016/j.jmb.2007.03.047. Epub 2007 Mar 24. PMID: 17512541.

22: Bhattacharjya S, Xu P, Wang P, Osborne MJ, Ni F. Conformational analyses of a partially-folded bioactive prodomain of human furin. Biopolymers. 2007 Jul;86(4):329-44. doi: 10.1002/bip.20748. PMID: 17477394. 23: Siddiqui N, Osborne MJ, Gallie DR, Gehring K. Solution structure of the PABC domain from wheat poly (A)-binding protein: an insight into RNA metabolic and translational control in plants. Biochemistry. 2007 Apr 10;46(14):4221-31. doi: 10.1021/bi061986d. Epub 2007 Mar 15. PMID: 17358048. 24: Volpon L, Osborne MJ, Topisirovic I, Siddiqui N, Borden KL. Cap-free structure of eIF4E suggests a basis for conformational regulation by its ligands. EMBO J. 2006 Nov 1;25(21):5138-49. doi: 10.1038/sj.emboj.7601380. Epub 2006 Oct 12. PMID: 17036047; PMCID: PMC1630411.

25: Volpon L, Osborne MJ, Borden KL. NMR assignment of human eukaryotic translation initiation factor 4E (eIF4E) in its cap- free form. J Biomol NMR. 2006;36 Suppl 1:65. doi: 10.1007/s10858-006-9051-8. Epub 2006 Jul 27. PMID: 16871426. 26: Su Z, Osborne MJ, Xu P, Xu X, Li Y, Ni F. A bivalent dissectional analysis of the high-affinity interactions between Cdc42 and the Cdc42/Rac interactive binding domains of signaling kinases in Candida albicans. Biochemistry. 2005 Dec 20;44(50):16461-74. doi: 10.1021/bi050846l. PMID: 16342938. 27: Osborne MJ, Siddiqui N, Landgraf D, Pomposiello PJ, Gehring K. The solution structure of the oxidative stress-related protein YggX from Escherichia coli. Protein Sci. 2005 Jun;14(6):1673-8. doi: 10.1110/ps.051358105. Epub 2005 May 9. PMID: 15883188; PMCID: PMC2253388.

28: Gutiérrez P, Li Y, Osborne MJ, Pomerantseva E, Liu Q, Gehring K. Solution structure of the carbon storage regulator protein CsrA from Escherichia coli. J Bacteriol. 2005 May;187(10):3496-501. doi: 10.1128/JB.187.10.3496-3501.2005. PMID: 15866937; PMCID: PMC1112004.

29: Osborne MJ, Siddiqui N, Iannuzzi P, Gehring K. The solution structure of ChaB, a putative membrane ion antiporter regulator from Escherichia coli. BMC Struct Biol. 2004 Aug 11;4:9. doi: 10.1186/1472-6807-4-9. PMID: 15306028; PMCID: PMC514712.

Michael J Osborne Curriculum Vitae 6

30: Gutiérrez P, Osborne MJ, Siddiqui N, Trempe JF, Arrowsmith C, Gehring K. Structure of the archaeal translation initiation factor aIF2 beta from Methanobacterium thermoautotrophicum: implications for translation initiation. Protein Sci. 2004 Mar;13(3):659-67. doi: 10.1110/ps.03506604. PMID: 14978306; PMCID: PMC2286745. 31: Osborne MJ, Venkitakrishnan RP, Dyson HJ, Wright PE. Diagnostic chemical shift markers for loop conformation and substrate and cofactor binding in dihydrofolate reductase complexes. Protein Sci. 2003 Oct;12(10):2230-8. doi: 10.1110/ps.03219603. PMID: 14500880; PMCID: PMC2366930. 32: Osborne MJ, Su Z, Sridaran V, Ni F. Efficient expression of isotopically labeled peptides for high resolution NMR studies: application to the Cdc42/Rac binding domains of virulent kinases in Candida albicans. J Biomol NMR. 2003 Aug;26(4):317-26. doi: 10.1023/a:102**********. PMID: 12815258.

33: Volpon L, Lievre C, Osborne MJ, Gandhi S, Iannuzzi P, Larocque R, Cygler M, Gehring K, Ekiel I. The solution structure of YbcJ from Escherichia coli reveals a recently discovered alphaL motif involved in RNA binding. J Bacteriol. 2003 Jul;185(14):4204-10. doi: 10.1128/JB.185.14.4204-4210.2003. PMID: 12837795; PMCID: PMC164884. 34: Ash J, Wu C, Larocque R, Jamal M, Stevens W, Osborne M, Thomas DY, Whiteway M. Genetic analysis of the interface between Cdc42p and the CRIB domain of Ste20p in Saccharomyces cerevisiae. Genetics. 2003 Jan;163(1):9-20. doi: 10.1093/genetics/163.1.9. PMID: 12586692; PMCID: PMC1462410. 35: Collins ES, Whittaker SB, Tozawa K, MacDonald C, Boetzel R, Penfold CN, Reilly A, Clayden NJ, Osborne MJ, Hemmings AM, Kleanthous C, James R, Moore GR. Structural dynamics of the membrane translocation domain of colicin E9 and its interaction with TolB. J Mol Biol. 2002 May 3;318(3):787-804. doi: 10.1016/S0022-2836(02)00036-0. PMID: 12054823. 36: Osborne MJ, Schnell J, Benkovic SJ, Dyson HJ, Wright PE. Backbone dynamics in dihydrofolate reductase complexes: role of loop flexibility in the catalytic mechanism. Biochemistry. 2001 Aug 21;40(33):9846-59. doi: 10.1021/bi010621k. PMID: 11502178.

37: Osborne MJ, Wright PE. Anisotropic rotational diffusion in model-free analysis for a ternary DHFR complex. J Biomol NMR. 2001 Mar;19(3):209-30. doi: 10.1023/a:101**********. PMID: 11330809. 38: Huntley JJ, Scrofani SD, Osborne MJ, Wright PE, Dyson HJ. Dynamics of the metallo-beta-lactamase from Bacteroides fragilis in the presence and absence of a tight-binding inhibitor. Biochemistry. 2000 Nov 7;39(44):13356-64. doi: 10.1021/bi001210r. PMID: 11063572.

39: Atkins AR, Osborne MJ, Lashuel HA, Edelman GM, Wright PE, Cunningham BA, Dyson HJ. Association between the first two immunoglobulin-like domains of the neural cell adhesion molecule N-CAM. FEBS Lett. 1999 May 21;451(2):162-8. doi: 10.1016/s0014-5793(99)00554-2. PMID: 10371158.

40: Moore GR, Cox MC, Crowe D, Osborne MJ, Rosell FI, Bujons J, Barker PD, Mauk MR, Mauk AG. N epsilon,N epsilon- dimethyl-lysine cytochrome c as an NMR probe for lysine involvement in protein-protein complex formation. Biochem J. 1998 Jun 1;332 ( Pt 2)(Pt 2):439-49. doi: 10.1042/bj3320439. PMID: 9601073; PMCID: PMC1219499. 41: Davy SL, Osborne MJ, Moore GR. Determination of the structure of oxidised Desulfovibrio africanus ferredoxin I by 1H NMR spectroscopy and comparison of its solution structure with its crystal structure. J Mol Biol. 1998 Apr 3;277(3):683-706. doi: 10.1006/jmbi.1998.1631. PMID: 9533888.

42: Wallis R, Leung KY, Osborne MJ, James R, Moore GR, Kleanthous C. Specificity in protein-protein recognition: conserved Im9 residues are the major determinants of stability in the colicin E9 DNase-Im9 complex. Biochemistry. 1998 Jan 13;37(2):476-85. doi: 10.1021/bi971884a. PMID: 9425068. Michael J Osborne Curriculum Vitae 7

43: Osborne MJ, Wallis R, Leung KY, Williams G, Lian LY, James R, Kleanthous C, Moore GR. Identification of critical residues in the colicin E9 DNase binding region of the Im9 protein. Biochem J. 1997 May 1;323 ( Pt 3)(Pt 3):823-31. doi: 10.1042/bj3230823. PMID: 9169618; PMCID: PMC1218388. 44: Osborne MJ, Crowe D, Davy SL, Macdonald C, Moore GR. NMR of paramagnetic proteins. Methods Mol Biol. 1997;60:233-69. doi: 10.1385/0-89603-309-0:233. PMID: 9276250. 45: Osborne MJ, Breeze AL, Lian LY, Reilly A, James R, Kleanthous C, Moore GR. Three-dimensional solution structure and 13C nuclear magnetic resonance assignments of the colicin E9 immunity protein Im9. Biochemistry. 1996 Jul 23;35(29):9505- 12. doi: 10.1021/bi960401k. PMID: 8755730.

46: Davy SL, Osborne MJ, Breton J, Moore GR, Thomson AJ, Bertini I, Luchinat C. Determination of the [Fe4S4]Cys4 cluster geometry of Desulfovibrio africanus ferredoxin I by 1H NMR spectroscopy. FEBS Lett. 1995 Apr 17;363(1-2):199-204. doi: 10.1016/0014-5793(95)00317-3. PMID: 7729544.

47: Davy SL, Breton J, Osborne MJ, Thomson AJ, Thurgood AP, Lian LY, Pétillot Y, Hatchikian C, Moore GR. MCD and 1H-NMR spectroscopic studies of Desulfovibrio africanus ferredoxin I: revised amino-acid sequence and identification of secondary structure. Biochim Biophys Acta. 1994 Nov 16;1209(1):33-9. doi: 10.1016/0167-4838(94)90133-3. PMID: 7947979. 48: Osborne MJ, Lian LY, Wallis R, Reilly A, James R, Kleanthous C, Moore GR. Sequential assignments and identification of secondary structure elements of the colicin E9 immunity protein in solution by homonuclear and heteronuclear NMR. Biochemistry. 1994 Oct 18;33(41):12347-55. doi: 10.1021/bi00207a001. PMID: 7918457. 49: el-Thaher TS, Bailey GS, Wilson MT, Osborne M, Moore GR. A 1H-NMR study of the interactions between rat tissue kallikrein and two peptide inhibitors. Biochim Biophys Acta. 1992 Nov 20;1160(2):235-8. doi: 10.1016/0167-4838(92)90013- 4. PMID: 1445951.

50: James R, Curtis MD, Wallis R, Osborne MJ, Kleanthous C, Moore GR. Specificity determinants of the interaction between colicin E9 and its inhibitor protein in Bacteriocins, Micocins and Lantibiotics (eds. R. James et al.) NATO ASI series H, Springer Verlag, Heidelberg, 181-201, 1992.

51: Cook MJ, Cracknell SJ, Moore GR, Osborne MJ, Williamson DJ. Solution phase 1H and 13C NMR assignments of some 1,4,8,11,15,18,22,25-Octa-alkylphthalocyanines.Mag. Res. Chem., 29, 1053-1060, 1991. 52: Cox MC, Osborne MJ, Thurgood AGP, Moore, GR. Structural characterisation of interprotein complexes involving cytochrome C. Journal of Inorganic Biochemistry 43(s 2–3):106.DOI:10.1016/0162-0134(91)84102-F In addition a Research News article has appeared in Science which describes a presentation given by Dr. Wright at the "NMR in Molecular Biology" conference held at Oxford in August 1997, describing my work on DHFR. References

Dr. Katherine Borden

Institute of Research in Immunology

and Cancer (IRIC),

Université de Montréal,

Pavilion Marcelle-Coutu, Chemin

Polytechnique,

Montreal, Qc, Canada

*********.******@*********.**

Dr. Peter E Wright

Dept. Molecular Biology

The Scripps Research Institute

10550 North Torrey Pines Road

Ca 92037, USA

Tel: 858-***-****

******@*******.***

Prof G.Moore

Department of Chemistry

University of East Anglia

Norwich, Norfolk

NR4 7TJ, UK

Tel: 016**-******

*.*****@***.**.**

Contact this candidate