Client Relationship Management

CAREER PATH

Worked as a Sr, Research Fellow at

“National Institute of Virology”,

Pune, India. With over 4.2 yrs of

experience in processing specimen,

diagnostics and analysis, offering

professional service to a laboratory

in need. Worked as guest lecturer

for Master of Science students in

Modern college, Pune, India.

Shilpa Bhalla

136 Cherry Street, Jersey

City, 07305

WORK EXPERIENCE

National Institute of Virology, India May, 2005- April 2010 Developed and maintained strong and beneficial internal and external business relationships.

Developed powerful marketing campaigns aimed at retaining and attracting customers.

Utilized online marketing tactics and managed goals for social media channels.

Increased compliance by educating Mentees on nutritional and lifestyle changes that they needed to make.

Developed a high level of trust with mentees by strategizing with, encouraging and guiding them during their journey through learning how to change diet and lifestyle.

Entrepreneurship at Modere

Certified Gut Health-Weight Loss mentor with Putting health at the top

(PHATT)

April, 2020-Present

SKILLS

Quest for challenges

Leadership

Good communication

Extremely knowledgeable in specimen analysis and testing. Proficient in data collection programs relevant to specimen processing.

Ability to work independently or with a group of people toward a common goal.

People skills necessary when collecting samples and specimens from patients.

Understanding of all safety standards regarding specimen collection and storage.

Experience working with hazardous chemicals and harmful substances.

Researched new types of testing or equipment available to Specimen Processing.

adxbd7@r.postjobfree.com

551-***-****

Modere, USA

PROFESSIONAL SUMMARY

PROFESSIONAL SUMMARY

Preparation of specimens for testing which meet the Laboratory's criteria. Performs daily maintenance and replenishes supplies and reagents as needed.

Request and pull samples from other locations for pending tests. Familiar with tube types, test request and medical terminology. Establish positive patient identification on sample or in reporting systems before proceeding with any further action.

Follow proper incident procedure in the event of any accident, specimen breakage, and spills.

Attends department trainings and meetings and interacts in two way communication to assist in process improvement.

Maintains compliance with all laboratory policies and procedures as well as safety and quality practices.

Lab Assistant

BioReference Laboratories, USA Jan, 2022-Present

PROFESSIONAL SUMMARY

EDUCATION HISTORY

Masters in Biotechnology, 2005, PTU, Punjab, India Bachelorette in Microbiology,2003, DAVV, Madhya Pardesh, India Extensive knowledge in Enteric Viruses (Hepatitis A virus, Rotavirus, Norovirus, Enterovirus)

Ability to plan, organize and prioritize work and meet deadlines. Self-motivated, quick learner and work well within the team. Have strong team building skills and has ability to handle all phases of a project life cycle.

Molecular Biology:

Nucleic acid extraction, Primer design PCR, nucleotide sequencing, Gel Electrophoresis, PCR, synthesis of cDNA.

Immunological Techniques: ELISA, Antibody purification, HRP conjugation, Neutralization assay

Bioinformatics:

BLAST, Phylogenetic analysis,

Egg inoculation: Egg inoculation via various routes Experiment samples preservation, recording, retrieving and organization

TECHNICAL SKILLS

PERSONAL STRENGTH

Serum samples collected during the years 2006-2009 were tested. Hepatitis A infection was detected in 10-33% of AES cases.

Infections were high up to 6 years of age and appeared to occur throughout the year. Fecal excretion of hepatitis A virus in 12-13% and Viremia in 31% cases indicate recent infection of HAV in hospitalized AES patients.

HAV IgM positivity observed in 17-64% CSF samples suggests possibility of HAV infection as a cofactor in increasing severity of any other infection or its association in causing AES. Fecal specimens from a total of 105 children less < 5 yrs of age, hospitalized for diarrhoea were collected during Jan-March 2009.

-40.8% specimens were detected positive for rotavirus by ELISA.

-75.8% were typed for VP7 (G) and VP4 (P) genes.

-65.5% of the specimens were detected with G & P types gene

-10.3% strains remained untypable for both the genes. Each of G1P[8] and G3P[8] represented 5.2% of each of the strains, respectively while none of the specimens showed presence of a common types.

G2P[4] and G4P[8].G9P[8], G12P[6] and G12P[8] were detected at a frequency of 15.7%, 21% and 10.5%, respectively. G and P types in mixed infections represented 42.1% of the strains. Out of 342 serum samples 284 were found to be positive for anti-HAV IgM. Two hundred and twenty three of 284

(78%) specimens from different regions (Southern (n=142), Western (n=54), Eastern (n=17) and Northern (n=10) were positive for HAV RNA by RT-PCR

Nucleotide sequencing of the PCR products indicated presence of genotype IIIA. Percent nucleotide variations in Indian strains ranged from 0 -3.4 in 5’NCR, 0-8.5 in partial VP1/2A junction and 0.7-10.5 in partial RNA polymerase regions as compared to GenBank IIIA strains. Full virus particles were visualized by electron microscopy while presence of viral RNA and its 11 segments were shown by RT-PCR and PAGE respectively.

The propagation of virus was monitored up to passage 6 and was found to be infectious to normal MA104 cell culture.

Allantoic and amniotic membranes of embryonated eggs supported replication of chicken rotavirus strain Ch2. The study adds to the base line data on adaptation and propagation of avian rotaviruses in embryonated egg system.

Organization: National Institute of Virology, India Project Title 1: Investigation of acute encephalitis cases for viral etiological agents Project Outcome:

Project Title 2: Hospital Based Surveillance for Rota Virus Disease and Strains Project Outcome:

a.

b.

c.

d.

Project Title 3: Genomic Analysis of Hepatitis A virus isolates from different geographic locations of India Project Outcome:

Project Title 4: Propagation of avian rotavirus Ch2 in embryonated hen’s egg (Part of Master’s program) Project Outcome:

Detection and characterization of hepatitis A virus in different clinical specimens collected from hepatitis A patients

Seroepidemiology of hepatitis A in animals from India Detection of rotavirus serotype specific antibodies in human sera Preparation of reagents for immunodiagnostics of Rotavirus infection Molecular characterization of untypable rotavirus strains from India Detection and molecular characterization of enteric viruses in hospitalized patients from Pune, western India Organization: National Institute of Virology, India Association with other Projects

International Publications

Title: Evidence of hepatitis A virus infection in the patients with acute encephalitis syndrome in Gorakhpur region, North India

Author: M. S. Joshi, B. V. Tandale, M. M. Gore, S. Bhalla, Y. K. Gurav, G. N. Sapkal, K. P. Kushwaha, A. C. Mishra, S. D. Chitambar,

Year of Publication: January 2018

Journal: Acta Virologica 62(02):208-213

Title: Characterization of GII.4 noroviruses circulating among children with acute gastroenteritis in Pune, India: 2005–2013

Author: Ruta Kulkarni, Amit Patel, Shilpa Bhalla, Preeti Chhabra, Sarah Cherian, Shobha D.Chitambar, Year of Publication: January 2016

Journal: Infection, Genetics and Evolution: Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases 37:163-173

Title: Exploring the concurrent presence of hepatitis A virus genome in serum, stool, saliva and urine samples of hepatitis A patients

Author: Madhuri S.Joshi, Shilpa Bhalla, Vijay, R.Kalrao, Ramchandra K.Dhongade, Shobha D.Chitambar, Year of Publication: April 2014

Journal: Diagnostic Microbiology and Infectious Disease 78(4) Title: Longer duration of viremia and unique amino acid substitutions in a hepatitis A virus strain associated with Guillain-Barré syndrome (GBS)

Author: Madhuri S. Joshi, Sarah S. Cherian, Shilpa Bhalla, Shobha D. Chitambar Year of Publication: May 2010

Journal: Journal of Medical Virology 82(6):913-9

Ray PG, Bhalla S, Chitambar SD. Development of ELISA for detection of rotavirus serotype specific antibodies in human sera. Poster presented at VIIth ‘Asia Pacific Congress on Medical Virology’ (APCMV) at India International Center, New Delhi, Nov 13-14, 2006

Shobha D Chitambar, Shilpa Bhalla*,Madhuri S Joshi*, Vishnuprasad.V**, P.SugandhiRao G.Arunkumar***

:Genomic Analysis of Hepatitis A virus isolates from different geographic locations of India. Poster presented at International Conference on Emerging and Re-Emerging Viral Diseases of the Tropics and Sub-tropics at IARI, New Delhi, 11th-14th Dec 2007.* National Institute of Virology, Pune,** Malabar Institute of Medical Science, Calicut Kasturba Medical College,Manipal.

Bhalla S, Buragohain M., Chitambar S.D. Propagation of avian rotavirus in embryonated hen’s egg. Poster presented at Virocon-2008, XVIII National Conference of Indian Virological Society, 12-13 Dec, held at PGIMER Chandigarh.

Shilpa Bhalla, Madhuri S Joshi,Vijay R Kalrao, Ramchandra K Dhongade, Shobha D Chitambar. Salivary and urinary presence of hepatitis A virus RNA in hepatitis A patients. Poster presented at Virocon 2010, XIX National Conference on “Recent Trends in viral disease problems and management”, 18-20 March 2010, held at Tirupati. Conference Publications:

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