Bhargavi Rayavarapu

Email: adx446@r.postjobfree.com

669-***-****

Summary

• Highly motivated, Innovative and goal-oriented researcher with a keen eye for detail.

• Extensive research experience in Microbiology, Plant biology, Food Microbiology, cell Biology and Molecular Biology

• Expertise on working with 3T3 cell lines, Mammalian renal epithelial cells (Th1), 293T cells and HEK cells.

• Packaging of lentiviral vectors and virus titer.

• Expertise on Plasmid isolation, Midi and Mini preparation

• Expertise on Flow cytometry to detect transfected and non-transfected cells

• Expertise on handling LC-MS analysis to detect bioactive compounds from bacteria

• Research experience on HPLC to detect bioactive compounds, IAA, GA from bacteria

• Well versed with analytical techniques to purify the proteins such as ion exchange and gel chromatography

• Expertise on Fermentation and optimization studies to enhance metabolites, enzymes from bacteria in culture media

• Well versed with the statistical tools like MATLAB, SPSS, Benchling for data analysis. Skills

ELISA • Statistical Tools • Handling of mammalian cell culture • Transfections • transformation • Flow cytometry • UV/Vis Spectroscopy • Mass Spectrometry • HPLC • Fluorescent Microscopy • Polymerase Chain Reaction (PCR) • Liquid Chromatography- Mass Spectrometry (LC-MS) Purification Techniques • Chromatography techniques • SDS PAGE

Work Experience Apr 2022 – Feb 2023

Intern at RSI (Regenerative Sciences Institute), Sunnyvale

• Expansion and maintenance of mammalian cell lines (CHO, 293T, Th1) suspension and adherent cells.

• Transfection experiments – Transfection of 293T cell and observing the transfection efficiency under fluorescent microscopy and flow cytometry.

• Packaging of lentiviral vectors and determined the virus titer

• Transformation and cloning experiments.

• Midi and Mini preparations.

Education

PhD in Microbiology Jan 2016- Oct 2021

Jain University, Bangalore, India

Title: “Study of Gamma aminobutyric acid (GABA) producing probiotic Lactic acid bacteria”.

• Research was mainly focused on production of GABA from LAB.

• Production of GABA was enhanced in soymilk compared with synthetic media

(MRS) by conducting optimization studies.

• Experimental data was authenticated by RSM and ANN models.

• GABA was identified by using HPLC and conformed by LCMS determining the molecular weight.

• Characterization of GAD enzyme was performed by extraction and purification of GAD enzyme and estimated the protein activity.

• Stability studies was performed to determine the production of GABA by LAB under stress conditions.

Master of Philosophy- M. Phil, Microbiology Aug 2014- Dec 2015 Jain University, Bangalore, India

Title: “Study of PGPR traits of Bacillus subtilis and AMF in Lycopersicum esculentum under salinity stress”.

• Research was focused on isolation of plant growth promoting rhizobacteria (PGPR) Bacillus and Glomus monosporum (AMF) from soil.

• Characterization of PGPR characters such as IAA, GA and antioxidant enzymes was studied.

• Plant studies was conducted to observe the effect of PGPR and AMF on Lycopersicum esculetum under salinity stress conditions.

Master of Sciences- M. Sc, Microbiology Jul 2008 – Jun 2010 Andhra University, Vishakhapatnam, India

Title: “Identification of bell pepper hybrid seeds using SSR markers”.

• Research was mainly focused on SSR markers. SSR markers were used to identify bell pepper hybrid seeds with parent seeds.

• It was identified by using PCR method and bands of hybrids and parent seeds were observed on SDS-PAGE.

Publications

1. Bhargavi Rayavarapu & Usha M.S. Optimization and comparison of ℽ-aminobutyric acid (GABA) production by LAB in soymilk using RSM and ANN models. Springer. Beni-Suef University Journal of Basic and Applied Sciences · Jan 12, 2021 2. Bhargavi Rayavarapu; Padmavathi T & Usha M.S. Statistical optimization of γ- aminobutyric acid production by response surface methodology and artificial neural network models using Lactobacillus fermentum isolated from palm wine. Elsevier: Biocatalysis and Agricultural Biotechnology · Nov 1, 2019 3. Bhargavi Rayavarapu & Padmavathi T. Evaluation of potential probiotic characters of Lactobacillus fermentum. Scientific Study & Research. Chemistry & Chemical Engineering, Biotechnology, Food Industry · Scopus Indexed Apr 1, 2019 4. Bhargavi Rayavarapu et al (2018). Screening of potential probiotic lactic acid bacteria and production of amylase and its partial purification. Elsevier: Journal of Genetic Engineering and Biotechnology · Dec 2, 2018

5. Bhargavi Rayavarapu & Padmavathi T. Effect of Bacillus sp and Glomus monosporum on growth and antioxidant activity of Lycopersicum esculentum (tomato) plants under salinity stress. Scopus. Plant Cell Biotechnology and Molecular Biology · Sep 11, 2016 6. Bhargavi Rayavarapu & Padmavathi T. Effect of Bacillus sp and Glomus monosporum on growth and antioxidant activity of bell pepper (capsicum annuum) under salinity stress. Scopus Journal of Global Agriculture and Ecology · Sep 7, 2016 7. Bhargavi Rayavarapu & Padmavathi T. Bacillus sp. as potential plant growth promoting rhizobacteria. International Journal of Advanced Life Sciences (IJALS) · Scopus. Jan 9, 2016

8. Padmavathi T & Bhargavi Rayavarapu. Recent Trends and Developments in Milk- Based Beverages (Book Chapter) Elsevier. Woodhead Publishing · Jan 1, 2019. Conferences

1. V G Bhargavi Rayavarapu & T Padmavathi. ‘Invitro evaluation of potential probiotic lactic acid bacteria from dairy foods”. Presented at Association of microbiologists of India, Jagadguru Sri Shivarathreeshwara university, Mysuru 2. V G Bhargavi Rayavarapu & T Padmavathi. ‘Evaluation of probiotic characters of Lb. fermentum from sour dough and its production of GABA using soya milk” presented a paper at state level seminar on Emerging trends in food processing and preservation held on 21

st

Feb,2018 at BMS College for women, Bangalore and awarded best paper presentation

3. V G Bhargavi Rayavarapu & T Padmavathi. ‘Evaluation of potential probiotic characters isolated from palm wine and its GABA production” presented a paper at National seminar on Recent Advances in Microbial Technology for Renewable Energy, Bioremediation, Health and Sustainable Agriculture (RAMT for REBHSA-2018) on 24-25 th

Jan 2018 at

Periyar University, Salem.

4. V G Bhargavi & T. Padmavathi. Optimization of GABA production by Lb. fermentum isolated from sour dough using soya milk presented at the Future India: Science

&Technology on 10-11 oct 2018 held at Oxford College of Sciences, Bengaluru 5. Poster entitled “Statistical optimization of GABA production by Lb. fermentum isolated from palm wine using soya milk” was presented in new vistas in science and technology for common food 11

th

Annual KSTA conference held on February 1-2-2019, at NMKRV college for women, Bengaluru.

Honors & Awards

Best Paper Presenter of the conference - B. M. S. College of Engineering Feb 2018.

Title: “Production of GABA from LAB in soymilk as culture media”. References

1. Name: Dr. Usha M S

Designation: Professor; Dept of Microbiology

Institution: Jain University

Email ID: adx446@r.postjobfree.com

Phone: +91-984*******

2. Name: Dr. Swetha S

Designation: Assistant Professor; Center for Incubation, Innovation Research and Consultancy Institution: Jyothi Institute of Technology

Email ID: adx446@r.postjobfree.com

Phone: +91-974*******

3. Name: Dr. Varalakshmi KN

Institution: Jain University

Designation: Professor and Dean of Sciences

Phone: +91-994*******

Emai ID: adx446@r.postjobfree.com

Contact this candidate