Julian O’Pharrow

919-***-**** adw28x@r.postjobfree.com

Professional Summary:

Responsible for novel assay design, development and implementation in support of basic, transitional, and clinical investigations.

Support of researchers in Designing, Optimizing, and Performing Flow Cytometry Experiments.

Working knowledge of GLP/GCLP requirements for conducting Pilot Studies and Clinical Trials.

Experience with FlowJo (TreeStar, Inc.) analysis software.

Perform laboratory duties in support of the receipt, processing, purification, analysis, and cryopreservation of hematopoietic cells obtained from umbilical cord blood for hematopoietic stem cell transplantation to ensure adherence to protocols and quality standards.

Education:

Bachelor of Science, Biology, North Carolina Central University, Durham, NC, 1996.

Skills:

Flow Cytometry • Quality Control• Immunoassays• Aseptic Technique• BD FACS Calibur

Flow Cytometer Operation• Cell Tissue Culture• Qualitative/ Quantitative Analysis• CLIA,

TJC, and CAP guidelines• GLP• GMP• Detail oriented• Excellent organization skills•

Ability to work effectively both independently and on a team

Professional Experience:

Interpace Pharma/Flagship Biosciences Nov 2020-Feb 2023

Flow Cytometry Technologist

Operation of Beckman Coulter Navios EX Flow Cytometer.

Perform all necessary QC and machine troubleshooting.

Analyze data (Kaluza Beckman Software).

Perform all sample (blood/bone marrow) benchwork.

Perform data entry into Laboratory Information System.

Effectively communicate with pathologists, office staff, and other staff members in a professional manner.

Performance of all assays according to client requisition and respective studies/trials.

Reason for unemployment: Death in family (Father passed away)

BioAgilytix Jun 2018- Jan 2019

Analyst III

Prepare worksheets with minimal supervision.

Prepare plate maps.

Serves as lead analyst on sample analysis projects.

Troubleshoot assays.

Compile/process data (make power export).

Prepare summary tables.

Peer review raw data.

Order reagents.

Participate in assay development and validation.

Run assays independently.

Adhere to provided method/directive.

Prepare buffers.

Use LIMS system.

Participate in sample analysis.

Pipetting.

Adhere to safety requirements.

Keep accurate inventory of supplies for assays

Responsible for accurate record keeping with respect to sponsor’s samples, data, and reports (documentation according GxP guidelines).

Duke University, School of Medicine Center for AIDS Research (CFAR) Jun 2016-Jun 2018

Laboratory Research Analyst I Flow Cytometry Core

Responsible for novel assay design, development and implementation in support of basic, transitional, and clinical investigations.

Focuses on assessing immunogenicity, immunocompetance, and immune reconstitution in human trials of HIV/AIDS vaccines, cellular therapies, and drug therapies.

Duties include sample preparation, analysis and data management, and assistance in the planning, development, and implementation of flow cytometric procedures applicable to research studies and clinical trials.

Monoclonal antibody selection and titer.

Cell surface and intracellular staining.

Cellular proliferation assays.

LSRII/Fortessa (BD Biosciences) Flow Cytometers.

FACSDiva (BD Biosciences) instrument software.

FlowJo (TreeStar, Inc.) analysis software.

Sample preparation, acquisition, data analysis and management, data reporting.

Support of researchers in Designing, Optimizing, and Performing Flow Cytometry Experiments.

Working knowledge of GLP/GCLP requirements for conducting Pilot Studies and Clinical Trials.

Experience in Data Management/Archival.

Microsoft Word, Microsoft Excel, and Microsoft PowerPoint.

Server-based Management and Archival of Data.

Duke Clinical Research Institute Durham, NC Jun 2014 – Jun 2016

Carolinas Cord Blood Bank

Research Technician I

Perform laboratory duties in support of the receipt, processing, purification, analysis, and cryopreservation of hematopoietic cells obtained from umbilical cord blood for hematopoietic stem cell transplantation to ensure adherence to protocols and quality standards.

Assist with general administrative activities to include, but not limited to, development of standard operating procedures.

Bring to the attention of the laboratory management staff concerns regarding individual cord blood units or indications that a local or remote site not in compliance with established CCBB standard operating procedures.

Receive and prepare cord blood unit and associated documents from local and remote collection sites; execute administrative functions to prepare cord blood unit for processing.

Receive and prepare maternal samples and associated documents from local and remote collection sites; prepare maternal samples for shipment to the testing facility for infectious disease testing.

Receive placentas and associated documents from local collection sites; vacuum seal and freeze placentas; ship of placentas to Fresenius processing facilities.

Unload and return cord blood shipping containers to remote collection sites.

Complete and maintain all quality control records pertinent to the Receiving Section of the laboratory.

Prepare cord blood unit working and confidential files for administrative, technical and medical review. Continually review and file paperwork and test results into the working file, ensuring that the file is completed and finished according to standard operating procedures.

Attend routine laboratory meetings and other meetings related to

Under the supervision of senior lab personnel, coordinate and participate in the preparation of culture media, chemicals, antibodies, pharmaceuticals, reagents, and solutions necessary for cell processing and cryopreservation.

Under the supervision of senior lab personnel, operate manual and automatic laboratory equipment used to count cells; stain cells; separate cells; wash cells; separate plasma, red cells, buffy coats, mononuclear cell populations and stem cells; and cryopreserve cells according to standard operating procedures for the Carolinas Cord Blood Bank. Under the supervision of senior lab personnel, evaluate collected laboratory data as outlined in the Carolinas Cord Blood Bank standard operating procedures; complete laboratory data forms and enter them into the electronic database for complete laboratory.

Temporary assignments:

DSM Pharmaceuticals Sep2013- Nov 2013

GlaxoSmithKline Nov 2013 - Jun 2014

Laboratory Corporation of America, RTP, NC Sep1998 - Sep2011

Center for Molecular Biology and Pathology Flow Cytometry Lab

Sr. Laboratory Technologist 9/2003 -9/2011

Laboratory Tech 2000 –2003

Hematology Lab Tech 1998 –2000

Immunophentypic analysis of leukemias and lymphomas by flow cytometry and image analysis.

Qualitative/quantitative analysis of histograms and patient data write up Operation, calibration, troubleshooting and maintenance of BD FACSCalibur flow cytometers for residual leukocyte analysis of leuko-reduced blood components.

Preparation of bone marrow, lymph node, and peripheral blood biopsies Immunophenotyping.

QC and maintenance of laboratory equipment

Maintained QC in accordance with CLIA, TJC and CAP guidelines.

CBC Analysis/instrumentation.

Coagulation analysis/instrumentation.

Slide preparation for morphology.

Triangle Labs, Inc – Durham, NC Aug 1996 – Jan 1997

Lab Tech

Environmental Analysis – air/soil/water.

Preparation and sterilizing of glassware for chemists.

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