Anna Puzinovici

*** ******* **, *******, ** M*H *B5

647-***-**** advlnl@r.postjobfree.com

SKILLS AND ABILITIES

Rich research experience in clinical diagnostic and molecular biology laboratories

Skilled in various molecular biology techniques such as Next Generation Sequencing, MALDI-TOF, PCR, Sanger sequencing, and DNA gels

Computer skills with Microsoft office ie. Excel, Word, PowerPoint

Time management skills

Great organization skills, problem solving skills and eager to learn

Conscientious, reliable, and very well organized

Excellent interpersonal, oral, and written skills

EDUCATION

2019 York University

Bachelor of Science (B.Sc.) in Biomedical Science

2022 University of Toronto

Data Analytics Bootcamp

Learning statistical analysis and python analytics including: APIs, Pandas, Matplotlib, NumPy, ScyPym

Covering databases and how to organize data using SQL, MongoDB, PostgreSQL/pgAdmin

Web visualization with HTML, CSS, Bootstrap framework, and Javascript charting

Other topics covered such as: Machine learning, Tableu, Hadoop

2022 Operations Associate

Worked with iPSCs to build cell banks for clients using techniques including cell imaging, taking cell counts, passaging, seeding and checking for confluency

Complete Batch Records (BRs), Logbooks, Forms, etc. under cGMP, and documents in detail through the use of SOPs and BRs for the processes and manufacturing steps

2019-2022 Public Health Ontario

Molecular Laboratory Technician

Performed next generation sequencing using the Illumina (MiSeq and Next Seq) and Nanopore platform and cleaned up NGS data

Validated a Covid-19 real-time PCR SNP assay for the K417 target in order to distinguish between the beta (B.1.1351) and gamma (P.1) variants of concern using limit of detection studies, as well as wrote the SOP

Compiled and performed preliminary data analysis on the data obtained from this validation

Trained and lead a team of laboratory technicians and medical lab technologists on how to run the assay as well as how to interpret the results, performed troubleshooting on problem cases

Performed next generation sequencing using the Illumina (MiSeq and Next Seq) and Nanopore platform

Operated on various automated RNA extraction platforms (MGI, Chemagic), performed manual DNA extractions on bacterial samples, and SDS-PAGE

Trained new employees on various RNA extraction platforms, setting up PCR as well as data analysis, and next generation sequencing

2018 Public Health Ontario

Summer Student

Evaluated sensitivity of 3 different types of sample collection swabs for detection of Bordetella by real time-PCR

Prepared primer and probe stock, calculated primer and probe dilutions

Performed Sanger sequencing

Gained experience in data management, analysis and writing SOPs

Developed strong theoretical and practical knowledge of molecular laboratory techniques

2017 Public Health Ontario

Summer Student

Quickly became skilled to use state of art technology, MALDI-TOF for identification of bacteria

Gained experience in using bioinformatics software for clinical application and data analysis

Performed molecular biological techniques such as DNA and protein extractions, and real time-PCR

Developed strong analytical and problem solving skills

Practiced fundamental microbiological techniques such as: working aseptically, serial dilution of bacterial samples and streaking

2017 York University

Laboratory Assistant

Gained experience in techniques such as PCR, gel electrophoresis, and western blotting

Maintained mammalian cell cultures and used microscopy to analyze cells for confluency

Became familiar with pipetting

Gained knowledge and experience in disinfection of experiment equipment and laboratory work areas

VOLUNTEER EXPERIENCE

2018-2019 St. Joseph Health Care Centre

Volunteer research assistant

Conducted a verification of a new sample collection swab, E-swab to facilitate automation of the microbiology diagnostics

Performed PCR on microbiological samples

Performed serial dilutions of microbiological samples and tested using Vitek

Built solid microbiology laboratory skills eg. serial dilutions, streaking microbiological samples

Had a deep understanding of the operation of a clinical microbiological laboratory

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