Data Entry Summer Intern

Gabriel O. Akerele

Durham, NC. ***** + 615-***-**** *********@*****.***

Skills

Assay Development

Wide range of laboratory techniques such as PCR, qPCR, Protein and Immunostaining assays, Cell Culture (Primary and Secondary Cells), Molecular Cloning, Classical and Modern Microbiological Techniques, Transcriptomics and Metagenomics.

Team Building, and Multidisciplinary Collaboration

Laboratory management

Live animal handling and welfare

Analytical Thinking

Proficient Data analysis using SAS, JMP, CLC and GraphPad Prism.

Competent Programming skills using Python for big data analysis.

Research and Publication

Generating, Summarizing and Presenting Reports

Experience

SENIOR RESEARCH SCIENTIST 01/2022 - Current

Targan Inc - Morrisville, NC

Developing, validating, executing, and reviewing protocols for in vitro assays, in line with the strategic vision and direction of the company

Collaborating with a diverse and cross-functional team of software and mechanical engineers to develop unique insights and solutions to challenges.

Assisting with Laboratory reorganization to accommodate a rapid expansion in human and material resources of Targan.

General laboratory maintenance, inventory management, and ensuring safety and sterility protocols are adhered to.

Communicating with skill, technical updates about team activities, progress, and challenges during company-wide meetings

POST-DOCTORAL RESEARCH ASSOCIATE 08/2021 - 12/2021

Meharry Medical College - Nashville, TN

Optimizing molecular cloning and transfection assays for localization and expression analysis of HIV pathogenesis factors with the aim of discovering novel drug targets

Maintaining various adherent and suspension mammalian cell lines such as Hela, 293T cells, SupT1 cells, and so on

Developing and optimizing immunocytochemical staining assays and flow cytometry analysis of mammalian cells

Generation and assessment of virus-like particles using immunological assays.

POST-DOCTORAL RESEARCH 08/2020 - 07/2021

Associate University of Georgia - Athens, GA

Maintenance and monitoring of lab inventory and order supplies, acquisition, setting up and management of laboratory equipment and resources associated with 2 biosafety level 1 and one biosafety level 2 laboratories.

Ensuring compliance with biological and chemical safety standards concerned with acquisition, handling, storage, and waste disposal.

Orientation and training of new students, including 2 PhD and 2 master's students in laboratory etiquette, regulations, and techniques.

Assistance in the development of standard laboratory operating procedures

Assistance of junior colleagues in troubleshooting protocols to maintain quality of experimental results and writing and editing technical reports.

Primary technician in charge of managing 6 field and 1 invitro study that cost more than $200,000 to $300,000, to test the efficacy of company products, such as direct fed microbials, in improving poultry production performance.

Formulation, in vitro and in vivo assessment of nanoparticle vaccines against necrotic enteritis for oral delivery

High throughput screening and bioinformatics analysis of RNA seq and microbiome data

Writing and proof-reading of manuscripts for publication.

GRADUATE RESEARCH ASSISTANT 08/2017 - 08/2020

University of Georgia - Athens, GA

Development, optimization, and execution of protocols for immunological assays including, but not limited to ELISA, in vitro T-cell proliferation, magnetic cell sorting, antibody staining, flow cytometry, nitric oxide production, and antigen-antibody neutralization.

Quantification, purification, and assessment of proteins using SDS-PAGE, western blot, and ion exchange chromatography.

Culture and extraction of extracellular, cell wall, and membrane proteins from bacteria

Mammalian secondary cell culture

Poultry primary and secondary cell culture

Development and execution of protocols for gene expression assays including RNA extraction, DNA extraction, primer design, PCR, and quantitative RT-PCR

Development and execution of microbiological assays such as bacteria enumeration from poultry gut using direct plate count, most probable gene number method (MPN), qPCR, and so on

Formulation and administration of poultry vaccines for necrotic enteritis studies

Design of trials and formulation of poultry experimental diets

Preparation of buffers and other reagents for laboratory use

Data collection, entry and analysis using statistical tools such as EXCEL, SAS, and JMP.

TEACHING AND LAB ASSISTANT 08/2019 - 12/2019

Instruction and supervision of 22 undergraduate students enrolled in Avian molecular biology.

GRADUATE RESEARCH ASSISTANT 01/2015 - 05/2017

Tennessee State University - Nashville, TN

Bioinformatic analysis of metagenomic, metabolomic, and RNA seq data using a variety of -omics tools

Development of software pipelines using python coding language for rapid and efficient analysis of large datasets

Formulation and administration of Salmonella vaccines in poultry for immunological studies

Development and execution of protocols for gene expression assays including RNA extraction, primer design, PCR, quantitative RT-PCR

Assessment of DNA and RNA quality using Nanodrop, bioanalyzer and agarose gel electrophoresis

Assistance with the use of bioreactor for growing bacteria

Assistance with microencapsulation of probiotic bacteria

Development and execution of protocols for ELISA.

SUMMER INTERN 08/2015 - 08/2015

Meharry Medical College - Nashville, TN

Observation of rats for social defeat stress related behaviors

Vaginal swabbing of rats

Cryo-sectioning, fixing and staining of rat brain tissue for microscopic examination

Giemsa staining of rat vaginal swabs and microscopic examination to assess pregnancy in female rats

Assistance with preparation of buffers and other reagents for laboratory use

Preparation of sucrose solutions for administration to rats.

GRADUATE RESEARCH ASSISTANT 09/2014 - 12/2014

Tennessee State University - Nashville, TN

Growth and preservation of mammalian tissue culture (glioblastomas)

Fixing and immunocytochemical staining of cells for fluorescent microscopy

Assistance with chemical treatment and lentiviral transduction of tumor cells for in vitro studies.

ASSISTANT LAB TECHNICIAN 02/2011 - 03/2012

International Institute of Tropical Agriculture - Nigeria

Assistance with culture media preparation for mycological studies

Data entry for analysis

Culture and observation of seeds for potential exotic pathogenic fungi species.

RESEARCH ASSISTANT 11/2010 - 02/2011

University of Ibadan Ibadan - Nigeria

Collection of fecal samples of pigs for laboratory analysis

Preparation of selective and differential media for culture of enteric bacteria

Antibiotic sensitivity testing using disc diffusion methods to screen for multidrug resistant E

Coli and Salmonella.

Education and Training

University of Georgia, Selvaraj - Athens, GA Ph.D

Poultry Sciences, 08/2020

Evaluating the in vitro characteristics and protective efficacy of chitosan nanoparticle vaccines against necrotic enteritis in broiler chickens.

Tennessee State University - Nashville, Nahashon, TN Master of Science

Animal Science, 05/2017

Expression and characterization of toll-like receptors and other immune genes in the adult guinea fowl using transcriptome analysis.

University of Ibadan - Nigeria Bachelor of Science

Microbiology, 10/2010

Antibiotic resistance patterns of E. coli Isolated from cattle fecal specimen from several farms and markets in Ibadan. Senior year Project Supervisor: Kayode F. Fashae, Ph.D.

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