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Olena Svitlova, M.S., ASCP., Medical Technologist

Springboro OH, 45066 • +1-937-***-****, United States Citizen adv2r4@r.postjobfree.com • linkedin.com/in/olena-svitlova-717320105/ EDUCATION

INSTITUTION: Wright State University, Dayton, OH

Master of Science (MS), Microbiology and Immunology (2017-2019) GPA: 3.8/4.0

• Thesis advisor: Nancy J. Bigley, Ph.D.

• Thesis title: Six-nine months long term culture of mouse bone marrow cells differentiated to macrophages and eosinophils.

INSTITUTION: Dnipropetrovsk Medical Academy, Dnipropetrovsk, Ukraine BSC. Medical Doctor.

CERTIFICATION

ASCP – Technologist in Microbiology; M(ASCP)CM 6865, 5/11/2021 Certificate of Achievement, Internet Security and You, Clark State Community College, March 15th, 2020, Dayton, OH, USA

CITI Program: Working with Mice in Research Settings, Wright State University, November 2018. PROFESSIONAL EXPERIENCE

Medical Technologist, CompuNet Clinical Laboratories, Miami Valley Hospital, 1 Wyoming St. Dayton, OH: November 2020 to the present time.

Duties: Molecular Technologist

• Collecting samples for PCR testing.

• Testing specimens using real-time PCR platforms:

• 1) QuantStudio 7 Pro systems, and GeneXpert (Cepheid);

• 2) Using the DiaSorin Molecular SimplexaTM COVID-19 Dirrect assay system;

• 3) Using FDA-cleared BioFire System FilmArray Panels test for viruses, parasites, yeast, and antimicrobial resistance genes.

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• Immuno Histo Chemistry (IHC) staining process – Dako technology: DAKO PT Link Instrumentation, Dako Autostainer Link.

• Prepare immune slides for staining.

• Performance of reagents used during the methods for testing.

• Antibody titration / validation. Preparation new antibody lots, detection kits, and ancillary reagents.

• ISH Diagnostics – Ventana Technology: Registration the reagents into the Ventana Computer system, use of the Ventana Prep Kits, prepare Immune slides for staining.

• Sideroblast – staining, diagnostic.

• Maintaining data analysis records.

• Entering data into a Lab information system.

• Preparing Lab equipment, Running QC.

• Identifying issues and troubleshooting, multiple tusking.

• Reporting results, communication with doctors (Pathology Department) regarding the performance of tests.

Clark State Community College Laboratory, Dayton, OH: January 2020 – August 2020 Duties: Medical Laboratory Instructor

§ Operation and maintaining of lab equipment.

§ Performed hematological and immunological tests.

§ Verified the authenticity of laboratory products.

§ Supervision of health professional about safety Lab policy (Biohazard Waste Disposal Methods, Contamination in Lab, Overview of the Microscope, Diagnostic of COVID-19 – PCR).

§ Antibacterial susceptibility testing (Kirby-Bauer Sensitivity Testing).

§ Performed Gel Electrophoresis Lab.

§ Inoculation and Isolation bacteria (used Selective / Differential media), Gram Stain Technique.

§ Identification bacteria (Gram Stain; Catalase Test; Hemolysis; Optochin/Bacitracin Test; PRY Test; Mannitol Salt Agar Test).

§ EnteroPluri Test Identification of Enterobacteriaceae. Wright State University Laboratory of Microbiology & Immunology, OH: January 2017 – December 2019

Duties: Laboratory Researcher/Laboratory Technician

§ Partnered with multidisciplinary researchers in the Microbiology and Immunology laboratory.

§ Quality checks on Western blots.

§ Testing samples using Real-Time PCR, QC.

§ Testing the solid content, density, turbidity, PH, acid content and water content of chemicals produced in the lab.

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§ Operation and maintaining of lab equipment.

§ Measuring the dimensions of the chemicals.

§ Mouse bone marrow collection, differentiation to Lymphoid / Myeloid stem cells.

§ Immuno Histo Chemistry (IHC) Staining / Analysis.

§ Immunofluorescence Technique.

§ Flow Cytometry Technique/Analysis.

§ Gramm/Giemsa/Wright/ H&E stains.

§ Performing multidisciplinary assignments.

Health Centre of Dermatology and Venereal Diseases, Dnipro, Ukraine: January 2001 – August 2014 Duties: Medical Technologist

§ Operation and maintaining of lab equipment.

§ Performed hematological, serological tests.

§ Diagnostics of infectious diseases (Use Real-Time PCR; Reverse Transcription PCR, ELISA, MALDI-TOF Mass Spectrometry).

§ Performed Flow Cytometry for diagnostic Chron’s disease.

§ Supervision of health professional about safety Lab policy (Biohazard Waste Disposal Methods, Contamination in Lab, Overview of the Microscope and other laboratory techniques).

§ Antimycobacterial/antifungal/antibacterial susceptibility testing

§ Consistently completed all required records/documentation pertaining to patient results. Reporting patient’s results.

§ Followed hospital policies and procedures for safety, health and patient privacy. REFERENCE

Nancy J. Bigley, Ph.D.

Professor, Neuroscience, Cell Biology & Physiology Professor, Pathology Medical Science Bldg 063B, 3640 Colonel Glenn hwy., Dayton, OH 45435-0001 E-mail: adv2r4@r.postjobfree.com

Telephone: 937-***-****, 937-***-****

Darlington O. Abrefa, MS, ASCP

Molecular Technologist of Department of Infectious Diseases of CompuNet Clinical Laboratories 2308 Sandridge Dr., Moraine, OH45439

E-mail: adv2r4@r.postjobfree.com

Telephone: 937-***-****

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Dawn P. Wooley, Ph.D.

Professor, Neuroscience Cell Biology & Physiology

Biological Science II 143, 3640 Colonel Glenn Hwy., Dayton, OH 45435-0001 E-mail: adv2r4@r.postjobfree.com

Telephone: 937-***-****

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