Animal Care Assistant Technician

Hongqiu (Autumn) Yang

Address: **** *** ****** ****, ***********, MD 21093

E-mail: ********@*****.***

Telephone: 443-***-****

PROFESSIONAL SKILLS

Microbiome: Metagenome and metatranscriptome NGS library preparation; Quantitative and Endpoint PCR; High-plex library normalization and pooling; Nucleic acid purification and protocol optimization and development; High molecular weight purification of DNA for long-read; 3rd generation sequencing experiments

Microbiology: Maintained and established bacterial culture; Phase contrast and epi-fluorescence Microscopy; Differential cell staining (Gram and Nugent) and interpretation

Genomics: Routine processes 16S pipeline samples collection; Specimen inventory; Total nucleic acid purification; Nucleic Acid QA/QC measurement; Genomic DNA size selection and QC

Oncology: Xenograft tumor model; Dosing (IV, SC, IP, IM, ID) for mice and rats; Sample collection (organs, tissue, plasma, and serum); Bleeding (orbital bleeding, facial bleeding, tail, and heart puncture); Perusing animals and observe animals

Production Research and Automation:

Automated liquid handling operation, programming, and troubleshooting of platforms:

Hamilton STAR, STARlet, Nimbus, LabElite ID,

Qiagen QIAsymphony, QiaAgility

STP Labtech Mosquito HV and Mosquito Genomics

Fluidigm BioMark HD

Research and Development:

High-density microfluidic qPCR array development for rapid turn-time microbiome profiling. Covid 19 testing .

Miniaturization and automation of metagenomic library construction

Automation of nucleic acid purification for bacterial isolates for high throughput genome sequencing

Specimen and sample tracking in custom laboratory informatics management system

Cell culture, ELISA, Western blot, RNA isolation/extraction from live cells, Protein extraction from liver cells, RT-PCR, BCA assay, Plasmid transfection, bacterial culture (E.coli), CFU.

Special Technical Development:

Adopt a new technology of Mass cytometry (CyTOF) to acquire clinical trial samples;

Running daily quality control (QC) and maintenance for Flow cytometry instruments.

WORKING EXPERIENCES

October 2021 - present: CyTOF technique Specialist SKCCC CyTOF Flow/Mass cytometry core, Johns Hopkins University School of Medicine

Independently operating CyTOF instrument of Hyperion and Helios.

Independently operating Flow instruments such as Cytoflex, BD LSR II, BD Fortessa.

Using CyTOF to run clinic samples in single cell suspension and tissue slides.

Acquisiting and collecting data for analysis and troubleshooting.

Performing daily documents record, inventory, lab management,

Ordering equipment and supplies.

May 2020- October 2021: Research Laboratory Specialist Microbiome Service Laboratory (MSL), Institute for Genome Sciences, University of Maryland Baltimore

Covid-19 patient sample tests following CDC guidelines

Patient samples transfer, RNA extraction, RT-qPCR

January 2018 - April 2020: Research Laboratory Assistant Microbiome Service Laboratory (MSL), Institute for Genome Sciences, University of Maryland Baltimore

Independently plan and conduct client experiments and microbiome projects

Handling genomics and high-throughput automation instruments

Maintain laboratory records and sample tracking

Produce analysis products and reports for individual custom projects.

Developed new methods and protocols used in the laboratory

Assist laboratory members and provide training for new members.

September 2009 - January 2018: Research Laboratory Assistant Institute for Genome Sciences, University of Maryland Baltimore

Sample collection and tracking

DNA/RNA extraction 16S rRNA gene sequencing pipeline

Develop and maintain research databases

April 2008- August 2009: Lab Assistant Technician Division of Cardiology, Department of Medicine, University of Maryland Baltimore

Perform laboratory techniques and experiments in mice and rats

Maintain records of animal care and use

Drug dosing for mice and rats.

February 2007-December 2007: Volunteer Department Oral Pathology, Medicine & Radiology, Indiana University School of Dentistry

Perform basic lab experiment techniques, including cell cultures and western blot.

EDUCATION

1997 Associates Degree, Southwest Law and Politics University of China, Chongqing, China

2014 Nursing Assistant Certificate, State of Maryland Board of Nursing, USA

PUBLICATION

1.Ludford K, Ho WJ, Thomas JV, Raghav KPS, Murphy MB, Fleming ND, Lee MS, Smaglo BG, You YN, Tillman MM, Kamiya-Matsuoka C, Thirumurthi S, Messick C, Johnson B, Vilar E, Dasari A, Shin S, Hernandez A, Yuan X, Yang H, Foo WC, Qiao W, Maru D, Kopetz S, Overman MJ. Neoadjuvant Pembrolizumab in Localized Microsatellite Instability High/Deficient Mismatch Repair Solid Tumors. J. Clin. Oncol. 2023, JCO2201351. doi: 10.1200/JCO.22.01351.

2.Shin SM, Hernandez A, Coyne E, Munjal K, Gross NE, Charmsaz S, Yuan X, Yang H, Ho WJ. CyTOF protocol for immune monitoring of solid tumors from mouse models. STAR Protoc. 2022, 4(1):101949. doi: 10.1016/j.xpro.2022.101949.

3.Zabransky DJ, Danilova L, Leatherman JM, Lopez-Vidal TY, Sanchez J, Charmsaz S, Gross NE, Shin S, Yuan X, Hernandez A, Yang H, Xavier S, Shu D, Saeed A, Munjal K, Kamdar Z, Kagohara LT, Jaffee EM, Yarchoan M, Ho WJ. Profiling of syngeneic mouse HCC tumor models as a framework to understand anti-PD-1 sensitive tumor microenvironments. Hepatology, 2022, 10.1002/hep.32707. doi: 10.1002/hep.32707.

4.France M, Fu L, Rutt L, Yang H, Humphrys MS, Narina S, Gajer P, Ma B, Forney LJ, Ravel J. Insight into the ecology of vaginal bacteria through integrative analyses of metagenomic and metatranscriptomic data. Genome Biol. 2022, 23(1):66. doi: 10.1186/s13059-022-02635-9.

5.Holm J, Humphrys M, Robinson C, Settles M, Ott S, Fu L, Yang H, Gajer P, He X, McComb E, Gravitt P, Ghanem K, Brotman R, Ravel J. Ultrahigh-Throughput Multiplexing and Sequencing of >500-Base-Pair Amplicon Regions on the Illumina HiSeq 2500 Platform. mSystems, 2019, 4. e00029-19.

6.Ma B, McComb E, Gajer P, Yang H, Humphrys M, Okogbule-Wonodi A, Fasano A, Ravel J, Viscardi R. Microbial Biomarkers of Intestinal Barrier Maturation in Preterm Infants. Frontiers in Microbiology. 2018, 9. 2755.

7.Neuendorf E, Gajer P, Bowlin A, Marques P, Ma B, Yang H, Fu L, Humphrys M, Forney L, Myers G, Bavoil P, Rank R, Ravel J. Chlamydia caviae infection alters abundance but not composition of the guinea pig vaginal microbiota. Pathogens and disease. 2015, 73. 10.1093/femspd/ftv019.

8.Bai G, Gajer P, Nandy M, Ma B, Yang H, Sakamoto J, Blanchard M, Ravel J, Brotman R. Comparison of Storage Conditions for Human Vaginal Microbiome Studies. PloS one. 2012, 7. e36934. 10.1371/journal.pone.0036934

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