Eboni C. Hosch

Work: aduywg@r.postjobfree.com ; Personal: aduywg@r.postjobfree.com 336-***-**** Winston-Salem, NC

EDUCATION

The University of North Carolina at Charlotte Charlotte, NC

Bachelor of Science in Biology, Minor in Africana Studies May 2017

The University of North Carolina at Greensboro Greensboro, NC

Post-Baccalaureate Student June 2020 – December 2021

PROFESSIONAL SUMMARY

Research Lab Technician II at Wake Forest University School of Medicine with 3.5+ years of experience in cancer research and 3 years of experience in lab management who is proficient in data collection and analysis and is committed to using proper technique and safe lab practices to achieve accurate results and maintain a clean and productive working environment.

TECHNICAL SKILLS

Mammalian Cell Culture

Cell Counting

In vitro Drug Treatments

MTT and Cell Titer Blue Assays

Doubling Time Experiments

Transfection

DNA/RNA Purification and Isolation DNA/RNA and Protein Quantification

DNA/RNA Concentration

Nuclear/Cytoplasmic, and Whole Cell Protein Extractions

DNA/RNA and Protein Quantification Endonuclease Digestion of DNA

DNA/RNA Oxidative Damage ELISA

Western Blot

RTqPCR

cDNA Synthesis

Plasmid Purification

Bacterial Inoculation

Caspase Glo 3/7 Assay

Cell Counting

GraphPad Prism 7, SoftMax Pro, ImageJ, UNIX

RESEARCH EXPERIENCE

Atrium Health Wake Forest Baptist

Research Lab Technician II and Lab Manager Winston-Salem, NC

Oversees new employee and undergraduate training in relevant assays to ensure they are independently proficient September 2019 – Present

Manages lab by placing orders to maintain inventory, making certain all needs are met to complete experiments

Carries out various molecular and cell biology protocols, biochemical assays, focusing on the effects of targeted cancer therapies

Ensures lab remains in compliance with laboratory safety requirements as stated by Environmental Health & Safety

In charge of keeping all laboratory equipment at proper standards necessary for research purposes, including contacting manufacturer’s tech support to troubleshoot or repair equipment

Manages cryo storage and cell bank organization, including maintaining liquid nitrogen resources

Developed and enforced safety protocols to protect against the spread of COVID-19 within the Alli lab

Point of contact for lab when Principal Investigator is out of office and helps make certain lab runs smoothly

Atrium Health Wake Forest Baptist Winston-Salem, NC

Research Lab Technician I May 2019 – September 2019

Performed multiple, anti-cancer drug treatments on cell lines with follow-up biochemistry and molecular biology assays

Assisted in maintaining a laboratory environment compliant with Environmental Health & Safety guidelines

Spearheaded lab maintenance by dishwashing, autoclaving, making solutions, and stocking lab with necessary materials for experiments in support of the functional laboratory operations

Developed protocols for effective lab management/essential lab functions

Prepared presentations and figures for dissemination of data and presented relevant articles for journal club

Wake Forest University School of Medicine Winston-Salem, NC

Post-Baccalaureate Research Education Program Participant August 2018-May 2019

Maintained various, diverse cell lines in tissue culture for experimental use

Subjected triple negative breast cancer (TNBC) cells to targeted therapeutic options via the MTT assay and analyzed data to produce an IC50 value to measure drug effectiveness

Tested BRCA1-mutated cancer cell lines against therapeutic agents via the MTT assay

Organized The Cancer Genome Atlas data via a Bioinformatics screen to compare NUDT1 mRNA expression levels in TNBC cells to normal breast cells

Mined and compiled data to highlight and compare trends within various cancer cell lines through the use databases such as Genomics of Drug Sensitivity in Cancer (GDSC) and GEO2R

Audited graduate level courses in STEM and presented journal club articles

Created and delivered a poster presentation at MAPRS conference which catered to the inclusion of minority and underrepresented groups

The University of North Carolina at Charlotte Charlotte, NC

Bioinformatics and Genomics Lab Research Assistant May 2016 – May 2017

Unraveled the oat genome by designing primers using Geneious Software to conduct Polymerase Chain Reaction (PCR) and

Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR)

Calculated expected product from PCR and RT-PCR to determine presence of alternative splicing within IDS-1 gene in oats

Extracted information about the oat genome from Basic Local Alignment Search Tool (BLAST) results

Implemented UNIX operating system to organize files containing oat genome data

PUBLICATIONS

“Dysfunctional BRCA1-mediated Repair of Oxidative DNA Damage Predicts Sensitivity to Elesclomol-based Therapeutic Regimens.” Maria Kammire, Eboni Hosch, Sujeeth Shanmugam, James M. Ford, and Elizabeth Alli, Wake Forest University School of Medicine, Department of Cancer Biology, Winston-Salem, NC 27157, USA, Stanford University School of Medicine, Departments of Medicine/Oncology and Genetics, 269 Campus Drive, Stanford, CA 93405, USA. In Preparation.

ABSTRACTS

Maria Kammire, Eboni Hosch, Sujeeth Shanmugam, James M. Ford, and Elizabeth Alli. Dysfunctional BRCA1-mediated Repair of Oxidative DNA Damage Predicts Sensitivity to Elesclomol-based Therapeutic Regimens. Wake Forest University School of Medicine, Department of Cancer Biology, Winston-Salem, NC, Stanford University School of Medicine, Departments of Medicine/Oncology and Genetics, Stanford, CA. In Preparation.

Eboni Hosch, Enzo Palma, Elizabeth Alli. Targeted Therapy for Triple Negative Breast Cancer. Department of Cancer Biology, Wake Forest University School of Medicine. Mid-Atlantic PREP & IMSD Research Symposium (MAPRS), May 2019, Winston-Salem, NC.

Eboni Hosch, Rachel Walstead, Adam Whaley, Jaime Sheridan, Jessica Schlueter. RT-PCR to Determine Alternative Splicing of Introns of IDS-1 in Oats. Department of Biological Sciences, University of North Carolina at Charlotte, Crop Sciences, General Mills, Department of Bioinformatics and Genomics, University of North Carolina at Charlotte, STEM Matters, p. 23, NC-LSAMP Annual Undergraduate Research Conference. October 2016, Charlotte, NC.

Eboni Hosch, Rachel Walstead, Adam Whaley, Jaime Sheridan, Jessica Schlueter. RT-PCR to Determine Alternative Splicing of Introns of IDS-1 in Oats. Department of Biological Sciences, University of North Carolina at Charlotte, General Mills, Manhattan, KS, Department of Bioinformatics and Genomics, University of North Carolina at Charlotte. Bioengineering REU Program, July 2016, Charlotte, NC.

PRESENTATIONS

Hosch, Eboni. “Targeted Therapy for Triple Negative Breast Cancer.” Mid-Atlantic PREP & IMSD Research Symposium (MAPRS), 22-23 May 2019,

Wake Forest University School of Medicine. Biotech Place Conference Center, Winston-Salem. Poster Presentation.

Hosch, Eboni. “RT-PCR to Determine Alternative Splicing of Introns of IDS-1 in Oats.” NC-LSAMP Annual Undergraduate Research Conference,

14 October 2016, Cone University Center, University of North Carolina at Charlotte. Poster Presentation.

Hosch, Eboni. “RT-PCR to Determine Alternative Splicing of Introns of IDS-1 in Oats.” Summer Undergraduate Research Symposium, 27 July 2016, University of North Carolina at Charlotte. Poster Presentation.

OUTREACH AND OTHER EXPERIENCE

Wake Forest Baptist Medical Center Winston-Salem, NC

Intern/Visiting Student – Shadowed various doctors in Department of Pathology October-November 2017

Extensively observed rotations in hematopathology, cytopathology, autopsy, and surgical pathology

Attended Tumor Conferences that discussed dynamic cases and brainstormed most effective methods of patient care

Quest Diagnostics Greensboro, NC

Shadowed Dr. Valerie Fields, MD, FCAP, Medical Director of Cytology July 2017

Observed technicians operate technology used in diverse testing done on blood and other bodily fluid samples

Surveyed the treatment process of specimen preparation for use on microscope slides

Examined tissue and cell samples through microscopes to understand origin of disease and diagnosis

Novant Health Forsyth County Medical Center Winston-Salem, NC

Shadowed Dr. Jaleema Speaks, MD, FACOG July 2015

Studied relevant medical terminology related to labor, delivery, circumcision and other surgical procedures

Interpreted monitors for positive variability in fetal heart rate and changes in uterine contractions during labor and delivery

State Employees’ Credit Union (SECU) House Winston-Salem, NC

Volunteer June 2017 – July 2018

Organized kitchen, dining area and storage rooms to provide a comfortable environment for patients and caregivers during their stay while being treated at local hospitals (Atrium and Novant)

Assisted with front desk and computational duties to welcome guests and ensure the organization of guest records

Turning Point Academy & Thomasboro Academy Charlotte, NC

Classroom Volunteer August 2015 – May 2017

Taught and facilitated workshops to increase college readiness for middle and high-school aged students at Title I schools

Developed mentor-mentee relationships with young ladies to provide advice regarding every day concerns

ADDITIONAL SKILLS

Laboratory Management & Maintenance

Organizational Efficiency

Oral & Written Communication (In-Person, Presentations, Email, Telephone)

Microsoft Office (Excel, Outlook, PowerPoint, Word, OneDrive, OneNote)

Contact this candidate