Clinical Lab Scientist
Resume:
NAME: Wu, Frances Whei-Min Fan
ADDRESS: **** ********** *****, *****, ** 95616
TELEPHONE: ***-**-****
CITlZENSHIP: U.S.A.
E-MAIL: aduv0j@r.postjobfree.com
CURRiCULUM VITAE FOR FRANCES W. M. FAN WU
Frances W. M. Fan Wu (Frances W.M. Fan, Frances Fan Kuo-old names). 2734 Brandywine Place, Davis, CA 95616
530-***-****, 530-***-****, 530-***-****(cell) U.S.A.
aduv0j@r.postjobfree.com, aduv0j@r.postjobfree.com
ACTIVITIES/AFFILIATIONS
Clinical Laboratory Scientist Supervisor, Integrated Molecular Diagnostics, Pathology Laboratory 4150 Truxel Road, Suite C, Sacramento, CA 95834
Clinical Laboratory Scientist II, Quest Diagnostics Molecular Diagnostics Laboratory, 3620 Northgate Blvd., Sacramento, CA 95834
Microbiology Laboratory Supervisor, Adventist Health Rideout Memorial Laboratory, 726 4th Street, Marysville, CA 95901.
Training & Education Clinical Lab Scientist Specialist, UCSF Medical Center, Clinical Lab, Blood Bank; 505 Parnassus Ave., San Francisco, CA 94143
Clinical Lab Scientist, UCSF Medical Center, Clinical Lab, Blood Bank 505 Parnassus Ave., San Francisco, CA 94143
Clinical Lab Scientist PM Supervisor, John Muir Hospital, Concord, CA Clinical Lab Scientist, Stanford Hospital Lab at Hillview Automation Lab, 3375 Hillview Ave., Palo Alto, CA 94304
QC Lead and Sr. Clinical Lab Scientist, Stanford Blood Center, Technical Services Department, 3373 Hill View Ave., Palo Alto, CA 94304
PM Operation Supervisor and Senior Clinical Lab Scientist, Kaiser Permanente (North), Dept. of Laboratory, 2025 Morse Ave.
Sacramento, CA 95825-2115
Clinical Lab Scientist, CHW Mercy San Juan Hospital, 6501 Coyle Ave. Carmichael, Sacramento, CA 95608; Mercy Methodist Hospital and Mercy Folsom Hospital, Sacramento, CA Clinical Lab Technologist, Clinical Labs, California Regional Primate Research Center, University of CA-Davis, Davis, CA 95616 and University of CA-Medical Center, Sacramento, CA 95814 CERTIFICATlON AND LICENSURE
Licensed since Oct. 25, 1996 State of California license no. (MTA 36998) for "Clinical Laboratory Scientist"
Certified since July, 1996 NCA Certification in "Clinical Laboratory Scientist" (certificate no. 9602215)
Certified since Sept. 1996 ASCP Certification in "Medical Technologist" Agricultural Pest Control Advisor License, State of CA (1981-1982; No. 4393). ANALYSIS/INSTRUMENTATION EXPERIENCE
Atomic Absorption:
Automated Analysis
Blood Bank
Chemiluminescent Assay:
Chloridometry:
Electrophoresis:
Enzyme Immunoassay (EIA):
Flame:
Flow Cytometry
Fluorescence Polarization:
Perkin Elmer
3300 (Flame)
and Seeman
(6FAA)
Atomic
Absorption
Spectrophotometer
Baxter Stratus II Immuno Assay
System, Dade Behring Stratus CS,
Beckman CX-7 and CX-9 Clinical
System ALX, Cell-Dyn 3500 and
1700, Baker-2000, Coulter STKS,
Electra 1400 C-Automated
Coagulation (Hemoliance-Hemostasis
from MLA and Ortho) and MLA
1000C, Vitros 250,750, 950. Axsym,
Coulter GenS, CA6000, Super UA,
Access, Infusion FS5.1, Sysmex XE,
Sysmex XT, Coulter GenS,
Maxm, Pentra XL-80, Coulter LH750,
Centaur, Advia 2400, Stargo,
Starsed, Atlas and UF J 00, Ortho
Summit for Chagas
Immucor ABS 2000, Tango (Biotest),
Provue, PK7200
Ciba Coming ACS: 180
Digital Chloridimeter Labcon Company
Beckman
Electrophoresis System
and Beckman Appraise
Densitometer
Abbot Quantum II Spectrophotometer
Flame Photometer 343
Iristrumentation Laboratory
Inc.
Beckton Dickinson
Immunocytometry Systems-
Model Calibur,
Coulter EPICS Profile II
Flow Cytometer
TDX Analyzer and TDX/FLX Analyzer
Gas Chromatography:
Liquid Chromatography:
Mass Spectrophotometry:
Nephelometry:
Osmometer
Radioimmunossay (RlA):
Slide stainer
Spectrophotometry:
Whole Blood Analyzer:
DNA thermal cycler
PCR Diagnostic
Hewlett Packard 5880 and 5890 Gas Chromatograph and Perkin Elmer Auto system Gas Chromatograph
Bio-Rad MDMS and Perkin Elmer Series 400 Liquid
Chromatograph
Hewlett Packard 5970 Series Mass Selective Detector. Beckman Arry 360 System Nephelometer
The Advanced MICRO-Osmometer (Mode J 3 MO)
Packard Cobra II Auto-Gamma Counter
Bayer Hema- Tek 2000
Perkin Elmer Lambda 3A UVIVIS Spectrophotometer
Nova SP-5, Synthesis 15, Coming A-278, Radiometer 700 Perkin-Elmer-Cetus
GeneXpert (Cepheid), Verigene
Beckman Walkaway (MIC testing)
Panther by Hologic
Tigris by Hologic
Cepheid PCR instrument-GeneXpert
ABI 7500 FAST Real Time PCR System
QuantStudio 7 PCR Thermal Cycler
PROFESSIONAL EXPERIENCE
From Aug. 15, 2022 till 1/13/2023: At Integrated Molecular Diagnostics, Pathology Laboratory as the Clinical Laboratory Scientist Supervisor to be in charge of the operation and providing technical aspects of PCR COVID, Flu A, Flu B, RSV to staff, performance evaluation for Staff, conducting validation project, writing/editing SOPs, Inventory management, Approving Staff’s time sheet in ADP System, Staff’s proficiency testing, etc.
From Sept. 27, 2021 till present: At Quest Diagnostic Clinical Lab – Molecular Diagnostic Lab as the Clinical Lab Scientist II, performing COVID PCR testing, CT/GC PCR testing, BV, CV/TV, HPV, HPVR, EHPV, EHPVR, SPHPV, SPHPVR, C. diff PCR by Cepheid instrument, etc. From Dec. 2,2019 till Aug. 10, 2021: At Adventist Health Rideout, Microbiology Department as the Supervisor, managing the operation, responsible for instrument validation (GBS Direct Assay on GeneXpert, Gram Negative
MIC panel validation on Beckman Walkaway), RPR and HIV validation, etc., Training Staff. In addition, Education Coordinator for CLS Trainee. From June 12, 2011 till June 30, 2017: At UCSF Medical Center, Blood Bank Dept., Performing training & educational activities for BB residents, medical students, & fellows, Bb limited license students, SFSU CLS licensing trainee students; Coordinating & Assessing BB staff proficiency and competency testing, BB SOP new/revision tracking, BB bench testing including type & screen, crossmatches, antibody identification, blood product components preparation (irradiation, FFP & CRYO thawing, splitting blood products, washing RBC blood products, etc.), and etc.
From August 3, 2009 till June 30, 2017: work as a Clinical Lab Scientist, in Blood Bank, Clinical Lab, UCSF Medical Center; performing blood bank testing including operating PROVUE instrument for type and screen, antibody identification, setting up cross matched blood, FFP and plateletpheresis for patient needs, and etc.
From April to July, 2008: Performing all areas of Clinical Lab testing at John Muir Hospital at Concord, CA, including blood bank, microbiology, chemistry and special chemistry, Hematology, Coagulation, Urine analysis, bone marrow slide preparation and staining, etc. From August 27, 2007 till April 9,2008: I work as a Clinical Lab Scientist, Stanford Hospital Lab at Hillview Automation Lab, 3375 Hillview Ave., Palo Alto, CA 94304 in the areas of Chemistry (Automated line of Centaur and Advia), Hematology (Automated line of Coulter LH 750), Coag (Stargo) and Urine Analyses (Automated Atlas and UF-IOO).
From June 12,2006 till August 26,2007: I worked as the QC Lead in QC Lab, Technical Services Department, Stanford Blood Center, 3373 Hill view Ave., Palo Alto, Ca 94304 in the areas ofQC testing of all the blood products, perform QC analyses, writing and presenting QC reports, validation plan, revision of SOPs, manufacturing platelet pheresis products and etc. From October 16,2000 till June. 2007: I worked as a Senior Clinical Lab Scientist in the Dept. of Laboratory, Kaiser Permanente (North), 2025 Morse Ave., Sacramento, CA 95825-2115 _in the areas of blood bank/transfusion, chemistry, hematology, coagulation, immunology, urinalysis, bacteriology, and bone marrow.
From October 31. 2004 till October. 2005: I work as the PM Operation Supervisor; in charge of the Clinical Laboratory operation at 2025 Morse Ave., Sacrament, CA 95825; including the DAV, FAO and PTW MOBs. I am also responsible of following up with the Member Concern; monitoring and preparing the QA reports for ENV, CRY and the requisition in compliance. From July. 1999 till May. 2005:I work as a Clinical Lab Scientist in the Clinical Lab, CHW Mercy San Juan Hospital, (from July, 2000 RSR-Regional Source Request Clinical Lab Scientist in Mercy San Juan, Methodist and Folsom Hospitals, Sacramento, CA in the following areas: Blood transfusion services, Chemistry analyses, Coagulation, Hematology, Serology (including HCG pregnancy testing, HIV testing, Clostridium difficile toxin assay, Group A Strep assay, Drug screening, Mono's assay), CKMB, Myoglobin and Troponin testing, and urinalysis, Gen probe for Chlamydia sp. and Neisseria gonorrhoeae analyses. From April. 1997 till Oct. 12, 2000: I work as a Clinical Lab Technologist(generalist) in the Clinical Labs, California Regional Primate Research Center, University of California-Davis, Davis, CA 95616 in following areas: Hematology, Immunology (ELISA and Flow Cytometry), Microbiology (Parasitology included), Chemistry and Urinalysis.
From Dec. 1996 till April, 1997: I worked as a Clinical Lab Technologist in the Clinical Lab, Department of Pathology, University of CA-Davis Medical Center in Blood Banking and Immunology Department From July 31st, 1995 to July 30, 1996. I obtained professional laboratory experience working as a medical technologist trainee in the following areas:
Immunology: Antibody titer by immunofluorescent stain (ANA, DNA); PEP, rEF, IEP, VDRL, RPR, MHA TP, flow cytometry, nephelometry, viscosity testing and etc. Microbiology: Urine bench work, respiratory bench work (samples including throat swab, nasopharyngeal, bronchial washing, etc.), blood culture and analysis, CSF, genital, mycology, and virology bench work (Gen probe, Clostridium difficile toxin assay); specimen processing, and mycobacterium identification in Public Health Laboratory. Special chemistry (Chem II):
T4, T3 resin uptake, hepatitis marker (HBsAg, HBcAg, anti-HBc IgM, anti-HBc, anti-HBs, Hepatitis A virus antibody, Hepatitis A virus antibody-IgM, Hepatitis C virus antibody), Alpha-fetoprotein, cancer antigen 125, carcino embryonic antigen, cortisal, hCG, prolactin, prostate specific antigen, testosterone, bilirubin analysis (Delta 450)-Amniotic fluid by spectrometric analysis, gastric analysis-gastric contents, IgE in serum, microalbumin in urine, mononucleosis test, prealbumin, sweat chloride analysis; anemia panel (vit B12, folic acid and ferritin), HbAIC; LH ( lutenizing hormone). Chem I: Chern 7 panel, chern 13 panel, chern 20 panel, osmometer to measure urine osmolality, blood gas and electrolytes (Nova Stat profile 5).
Hematology and coagulation: CBC, differential, reticulocytes count, blood smear and staining, manual differential, platelet count, eosinophil, hematocrit, hemoglobin (Hgb), osmotic fragility, acid and alkaline Hgb electrophoresis, abnormal Hgb by HPLC, LAP, CSF fluid and synovial fluid, semen analysis, ESR, cryoglobulin, Hgb F quantitative analysis by alkaline denaturation, sugar water test for PNH, Donath-Landsteiner test for PCH, staining for malarial parasites, PT, APIT, coagulation factor assay, Thrombin time, functional protein C and protein S, euglobulin lysis time, DRVVT, bleeding time, inhibitor assay, fibrinogen.
Toxicology: therapeutic drug monitoring (TDM), acid-neutral drug extraction and base drug extraction using GC and GC-Mass for analysis. volatile drug screen, acetaminophen assay by HPLC, lithium analysis by AA, lead analysis by flame graphite AA, urine drug abuse monitoring by CX 7. Blood banking: ABO and Rh typing, antibody screen and typing, cross-matches and etc. Phlebotomy: blood drawn for both inhouse and out patients. Bone marrow lab: bone marrow slide making. bone marrow differential, cytochemical staining by PAS, peroxidase, Sudan black B. NSF (non-specific esterase stain), specific esterase stain, and iron staining
(Prussian Blue Reaction).
REFERENCES
Available upon request.
EDUCATION
I. Medical Technologist training at University of California, Davis. Medical Center Dept. of Pathology. Clinical Lab, Sacramento, CA July 1995-July 1996 2. M.S. Dept. of Botanical Sciences (Plant Pathology), Univ. of Hawaii, Honolulu, Hawaii 1975-1977
3. B.S. Dept. of Plant Pathology, National Chung-Hsing Univ., Taichung, Taiwan. R. o. C. 1970-1974
SPECIAL EDUCATION
I. "DOS", "LOTUS-123" course trained at Univ. of CA, Davis 1990 3
2. "Database" course training for literature search at Univ. of CA, Davis 1990 3. "SYST AT" course training at SYST AT Co., 1800 Shennan Ave., Evanston, Illinois 6020 I 1990
4. "DNA manipulation" training course at Univ. ofCA, Davis 1988 (lecture); 1989 (lab)
5. "Special problems in plant pathology" from Dept. of Plant Pathology, Cornell Univ., Ithaca, N.Y. 1977
6. Computer programming course (Fortran IV), Research Institute of Electrical Engineering, National Taiwan Univ., Taipei, R.O.C. 1974 PROFESSIONAL TRAINING
I. Flow Cytometry.
California Regional Primate Research Center, Univ. ofCA, Davis Davis, CA 95616
1997 -the present
2. DNA manipulation on nematodes and tomato (PCR, RFLP, DNA sequencing, cloning) Dept of Nematology, Univ. ofCA, Davis, CA 95616
1990-1995
3. Parasitology in mammal parasites of Bolivia.
Dept of Nematology, Univ. ofCA, Davis, CA 95616
1991-1995
4. Nematode culturing (greenhouse pots and ex-plant tissue), behavior, physiology, and biochemistry; Dept. of Nematology, Univ. ofCA, Davis, CA 95616
1983-1992
5. Plant tissue culture, cell biology;
Calgene Incorp., 1910 5th st., Davis, CA 95616
1982-1983
6. Immunofluorescence technique, soil microbiology; Dept. of Microbiology, Univ. of Hawaii, Honolulu, Hawaii 968**-****-****
7. Transmission electron microscopy;
Institute of Botany, Academia Sinica, Taipei, Taiwan, R.O.C. 1978-1980
8. Scanning electron microscopy;
Dept. of Plant Pathology, Cornell Univ., Ithaca, N.Y. 1977
California Dept. of Agriculture, sacramento, CA
FAI at Univ. ofCA, Davis, CA 95616
1982
9. Photography;
Institute of Botany, Academia Sinica, Taipei, Taiwan, R.O.C. 1975
10. Computer programming and data processing;
Research Institute of Electrical Engineering, National Taiwan Univ., Taipei. Taiwan, R.O.C. 4
1974
II. Protein and isoenzyme analysis;
Dept. of Biochemistry, U.S. Naval Medical Research Unit No.2, Taipei, Taiwan, R.O.C.
1973
PROFESSIONAL WORK EXPERIENCE
1. Clinical Lab Scientist Supervisor, Integrated Molecular Diagnostics, Pathology, Inc., Sacramento, CA 95834
2. Clinical Lab Scientist II, Quest Diagnostic Molecular Diagnostic Lab, Sacramento, CA 95834 3. Microbiology Lab Supervisor, Adventist Health Rideout, Marysville, CA 95901 4. Clinical Lab Scientist/Training & Education CLS Specialist, UCSF Medical Center Clinical Lab, Blood Bank Department, San Francisco,
2. Clinical Lab Scientist PM Supervisor, John Muir Hospital, Concord, CA 2008 3. Clinical Lab Scientist, Stanford Hospital Lab at Hillview Automation Lab, Palo Alto, CA 943**-****-****
4. QC Lead and Senior Clinical Lab Scientist, QC Lab, Technical Services Department, Stanford Blood Center, Palo Alto, CA 943**-****-****
5. PM Operation Supervisor and Senior Clinical Lab Scientist, Dept. of Laboratory, Kaiser Permanente (North Valley), Sacramento, CA 958**-****-**** 6. Clinical Lab Scientist Clinical Lab, CHW Mercy San Juan, Methodist and Folsom Hospitals, Sacramento, CA95608 1998-2005
7. Clinical Lab Technologist: Clinical Labs, California Regional Primate Research Center, Univ. of CA, Davis, Davis, CA 956**-****-****
8. Clinical Lab Technologist: Clinical Lab, Dept. of Pathology, Univ. of CA, Davis, Medical Center, Sacramento, CA95814 1996-1997
9.Medical Technologist Trainee: Dept. of Pathology, Univ. of CA, Davis, Medical Center, Clinical Lab 1995-1996
10. Staff Research Associate IV: Dept. of Nematology, Univ. of CA, Davis, CA 956**-****-****
11. Research Associate: Calgene Incorp., 1910 5th St., Davis, CA 956**-****-****
12. Agricultural Inspector: Dept. of Food & Agriculture, State ofCA, Lab Services/Plant Pathology, 1220 N St., Sacramento, CA 1982
13. Plant Pathologist: Symbex of Calif., 1104 N. School St., Stockton, CA 198*-****-**. Research Associate: Dept. of Microbiology, Univ. of Hawaii, Honolulu, Hawaii 198*-****-**. Assistant Research Fellow: Institute of Botany, Academia Sinica, Taipei, Taiwan. R.O.C. 1978-1980
16. Research Assistant: Institute of Botany. Academia Sinica, Taipei, Taiwan, R.O.C. 1975 5
17. Medical Student Trainee: Dept. of Biochemistry, U.S. Naval Medical Research Unit No.2, Taipei, Taiwan, R.O.C. 1973
CURRICULUM ACTIVITIES
1. Clinical Lab Scientist Supervisor, Integrated Molecular Diagnostics, Pathology Laboratory, Sacramento, CA 95834. In charge of Laboratory Operation, providing technical aspects for PCR testing of COVID, Flu A, Flu B, RSV, Conducting validation projects, writing/editing SOPs, Approving Staff time sheets in ADP system, performing Staff evaluation, Staff proficiency testing, Inventory management, etc. 2. Clinical Lab Scientist II, Quest Diagnostic Molecular Diagnostic Lab, Sacramento,CA. Performing PCR testing using various PCR instruments, i.e. Panther, Tigris, GeneXpert for COVID, CT/GC, HPV, HPVR, EHPV, EHPVR, SPHPV, SPHPVR, C. diff, etc.
3. Microbiology Laboratory Supervisor, Adventist Health Rideout, Marysville, CA, Managing the operation of Microbiology Laboratory, validation for new tests, training staff, working on microbiology bench.
4. Training & Education Clinical Lab Scientist Specialist, UCSF Medical Center Clinical Lab, Blood Bank Dept., Performing training & educational activities for BB residents, medical students, and fellows, BB limited license students, SFSU CLS licensing trainee students; Coordinating and assessing BB staff proficiency and competency testing, BB SOP new/revision tracking, Blood Bank bench testing 4. Clinical Lab Scientist, UCSF Medical Center Clinical Lab, Blood Bank Department, performing Blood Bank testing, including type and screen, antibody identification, cross match; setting up blood products including packed cells, FFP, plateletpheresis, and irradiating blood products for patient needs, and etc.
5. Clinical Lab Scientist, Stanford Hospital Lab at Hillview Automation Lab, working in Chemistry, Hematology, Urine Analyses and Coagulation automated line and testing. 6. QC Lead and Sr. Clinical Lab Scientist: QC Lab, Technical Services Department, Stanford Blood Center, working in plateletpheresis splitting, QC testing of all blood products, assigning Staff in QC Scheduling, writing and presenting QC reports at monthly QA meeting, validation plans, revising SOPs, QC data analyses and etc.
7 .PM Operation Supervisor and Senior Clinical Lab Scientist: Dept. of Laboratory, Kaiser Permanente(North), working in the areas of blood bank/transfusion service, chemistry, hematology, coagulation, urine analysis, body fluid, immunology, microbiology and bone marrow. 8. Clinical Lab Scientist: STAT Lab, CHW Mercy San Juan, Methodist, Folsom Hospitals, working in the areas of Transfusion Services, Hematology, Chemistry. Coagulation, Serology and Urinalysis. 9. Clinical Lab Technologist: Clinical Labs, California Regional Primate Research Center, Univ. of CA, Davis, working in the area of Hematology (performing CSC-electronic cell count, plasma protein and fibrinogen, making blood smears, reading differentials and reticulocytes, cross-matches for blood transfusion, urine and body fluids analyses), Immunology (running ELISA to screen Herpes antibodies, running flow cytometry), Microbiology (identifying import pathogens from the swabs of rectal and other anatomic sites), Parasitology (identifying parasites from stools, stool concentration and detecting Cryptosporidia and Giardia using immunofluorescentantibody technique), Chemistry (quality control for the whole blood analyzer-Nova SP 5, processing specimens to be sent out for chemistry testing. and blood banking).
10. Clinical Lab Technologist: Clinical Lab, Dept. of Pathology, Univ. ofCA, Davis, Medical Center. Working in the areas of Blood Banking and Immunology. 11. Medical Technologist Trainee: Dept. of Pathology, Univ. ofCA, Davis, Medical Center, Clinical Lab. Extensive training provides both basic knowledge and bench working experience in the areas of microbiology, hematology, chemistry, immunology, blood bank, coagulation, toxicology, urine analysis and phlebotomy.
12. Staff Research Associate IV: Dept. of Nematology, Univ. ofCA, Davis. Extracting DNA from tomato leaves and conducting Polymerase Chain Reaction (PCR) for 15 tomato lines using RAPD primers, screening for nematode resistant gene genetic markers located on chromosome 6( as a result, REX genetic marker was found by me); extracting DNA from cyst nematode iHeterodera cruciferae, H. schachtii) for PCR analysis detecting genetic difference among different species and populatios of cyst nematodes, investigating genomic structure (single or multiple gene copies) by RFLP; extracting DNA from insect nematodes-Heterorhabditis spp.(H. argentina, H. bacteriophora H. megidis, H. hawaiiansis, H. tasmania, H. indicus, H. zealandica, and etc.) and Steinernema carpocapsae, S. glaseri, and etc.) for peR analysis, and non-radioactive DNA sequencing to establish phylogeny trees for taxonomic studies. The above research activities include techniques of PCR, agarose gel electrophoresis, ligation, transformation, radioautography, DNA extraction, restriction enzyme digestion, data analysis using PAUP and McClade for phylogeny trees. 6
Isozyme analysis (esterases, Malic dehydrogenases) using Phastgel system was conducted on Root- knot nematodes (Meloidogyne spp.) and cyst nematodes (Heterodera spp.) for identification purposes.
DNA was extracted from several different species of root-knot nematodes in order to conduct PCR analysis on the DNA to differentiate them, DNA sequencing was carried out afterwards on those unique PCR DNA fragment which was specific for each species so that set of specific primers could be selected for identifying different species of root-knot nematodes at the molecular level. Biodiversity of mammal parasites in Bolivia. Parasites (pin worms, trematodes, whip worms, Trichostrongyloidea, etc.) collected from 'the stomach, small intestent, cecum of mammals
(Akodon, Ctenomys, Oryzomys, Oligoryzomys, etc.) are sorted and identified in order to establish phylogeny trees to study the biodiversity. Nematode (Heterodera schachtii, Xiphinema index, Tylenchulus semipenetrans, Criconemella xenoplax, etc.) pot culturing in greenhouse and ex-plant tissue culturing (Pratylenchus vulnus), conducting research on nematode behavior, physiology, and biochemistry,responsible for lab and greenhouse management, maintaining Hoagland plant nutrient system, computer data analysis.
13. Research Associate: Calgene Incorp. Associate research in the plant cell biology, tissue culture, protoplast preparation and microinjection.
14. Agricultural Inspector: Dept. of Food & Agriculture, State of CA, Lab Services/Plant Pathology. Disease diagnosis of plants and scanning electron microscopy (JOEL 35C & lSI 130) studies of diseased samples (corn smut, cereal rust). 15. Plant Pathologist: Symbex of CA. Establishment of microbiological lab for isolation and production of plant growth promoting rhizo-microorganisms (Yeast, Bacillus subtilis, CLostridium sp., Streptomyces sp., etc.), and plant disease diagnosis. 16. Research Associate: Dept. of Microbiology, Univ. of Hawaii. To assist in the study of tropical soils & their effects on the ecology of various nitrogen fixing bacteria by: identifying specific organisms in situ, plant host & soil environment, through use of immunological techniques
(include application of immunofluorescent technique in tagging & examining bacteria with antibodies & of immunodiffusion tech in developing & using immune serums from live animals), & assessing the viability of bacteria by plant infection under sterile conditions. 17. Assistant Research Fellow: Institute of Botany, Academia Sinica, Taipei, Taiwan, R.O.C. Investigate the inflation mechanism of a nematode trapping ring of the fungus- Arthrobotrys dactyloides; Examine the ultra-fine structure of the host-parasite interface of Peronospora brassicae
(downy mildew fungus) on Raphanus sa/NUS (turnip leaves) using transmission electron microscope
(Hitachi IIA); Conduct research on the disease control ofpaulownia witches' broom disease by antibiotical treatment with Achromycin & Doxycycline. 18. Research Trainee: Dept. of Plant Pathology, Cornell Univ., Ithaca, N.Y. Conduct the revival of Sclerotinia spp. & Botrytis spp. in culture: Study the degree of wettability of conidia of Botrytis cinerea & B. alii; in terms of possible ecological role in the natural environment (including the observations of the surface structure of spores with scanning electron microscope- AMR 900); Routine lab works-transferring cultures, single sporing, literature search, etc.). 19. Research Assistant: Institute of Botany, Academia Sinica, Taipei, Taiwan, R.O.C. Purification of the Garlic Stripe Mosaic Virus; Cultivation of the Mycoplasma-Like-Organisms from the sweet potato witches' broom diseased leaves & stems & the sugarcane white leaf diseased stems; transmission electron microscopy (Hitachi II A).
20. Medical Student Trainee: Dept. of Biochemistry, U.S. Naval Medical Research Unit No.2,Taipei, Taiwan, R.O.C. Identification of the genotype difference of the same phenotype of human blood & sea snake venom by electrophoresis.
7
REPORT AND PUBLICATION
I. 1994. Williamson, V. M., Ho, J. Y., Wu, F. F., Miller, N., and Kaloshian, I. A PCR-based marker tightly linked to the nematode resistance gene. Mi. in tomato. Theor. App!. Genet.87:757-763. 2. 1994. Williamson, V. M., Caswell-Chen, E. P., Wu, F. F., and Hanson, D. 1994. PCR for nematode identification. In: F. Lamberti, C. DeGorgi, and D. McK. Bird (eds), "Advances in Molecular Plant Nematology". pp.119-128.
3. 1994. Gardner, S. L., Wu, F. F., Thomas. K., Kaya, H. K., and Caswell-Chen, E. P. Phylogeny of insect pathogenic nematodes of the Genus Heterorhabditis (Nemata: Heterorhabditidae) from analysis of26s rDNA sequences and random amplified DNA polymorphisms. Manuscript in preparation for submission to Molecular Phylogeny and Evolution Journal. 4. 1992. Caswell-Chen, E. P., Williamson, V. M. and Wu, F. F. Random amplified polymorphic DNA analysis of Heterodera cruciferae and H. schachtii populations. J.ofNematology 24(3):343-351..
5. 1992. Wu, F. F., Stachyra, A., Viglierchio, D. R. The capacity of soil microbial isolates from non-fumigant nematicide stressed Heterodera schachtii greenhouse culture stocks to inactivate non-funigant nematicides. Fundam. appl. NematoL 15(3): 193-200 .. 6. 1989 Viglierchio, D. R. & F. F. Wu. Selected biological inhibitors for Heterodera schachtii control. Nematropica 19(1):75-79.
7. 1989 Viglierchio, D. R., S. M. Brown, and F. F. Kuo. Adaptive responses of Heterodera schachtii populations to nematicidal doses of -ionfumigant nematicides after stressing with sublethal doses. Revue de Nematologie 12:133-138.
8. 1988 Yamashita, T. T., D. R. Viglierchio and F. Fan-Kuo, Nonfumigant nematicide conditioned populations of Criconemella xenoplax and their responses to subsequent treatments. Revue Nematology 11(4):429-435.
9. 1988 Yamashita, T.T., D. R. Viglierchio, and F. F. Kuo. The role of microbial populations from long-term non fumigant nematicide treated soils on Heterodera Schachtii nematode trials. Revue de Nematologie 11(3):351-358.
10. 1983 Kuo (Wu), F. F. "Identification of smut fungi of regulatory significances by scanning electron microscopy." Abstract in the American Phytopathological Society, Pacific Division meeting held in March (23-24), 1983 at UC Berkeley.
II. 1983 Kuo (Wu), F. F. "Com smut" in California Plant Pest & Disease Report voI.2(1):2. 12. 1980 Kuo (Wu), F. F. "The inflation mechanism of the constricting ring ofa nematode trapping fungus-Arthrobotrys dactyloides". Institute of Botany, Academia Sinica, Taipei, Taiwan, R.O.C. 13. 1980 Kuo (Wu), F. F."The chemical control ofpaulownia witches' broom disease by Achromycin & Doxycycline" (presented in the poster session ofthe Third Conference ofInternational Organization of Mycoplasmology held from Sept. 2-9, 1980 in South Dakota, U.S.A.). 14. 1979 Kuo (Wu), F. F. "Fine structure of the host-parasite interface of Peronospora brassicae on Raphanus sativus". Institute of Botany, Academia Sinica, Taipei, Taiwan, R.O.C. 15.1977 Kuo (Wu), F. W. M. F. M.S. thesis-Etiology of fleck & spot development on Dendrobium blossom blight caused by BOlly tis spp. 1977. Dept. of Botanical Sciences (Plant 8
Pathology). Univ. of Hawaii Honolulu, Hawaii.
EXTRACURRICULAR COURSES TRAINING
I. Chemical and Cellular Immunology (MMI 107,4 units) University of California-Davis
Winter quarter, 1995
Grade: B
2. Comparative Hematology (PMI 101,2 units) and Lab (PM! 198, 1 unit) University of California-Davis
Spring quarter, 1995
Grade: A
3. Quantitative Analysis of Chemistry (CHEM 5, 5 units) American River College
Spring semester, 1995
Grade: A
4. Real Estate Financing
Sacramento City College
Spring, 1992
Grade: A
5. Financial Accounting
Sacramento City College
Fall, 1991
Grade; A
6. Principles of Real Estate
Sacramento City College
Spring, 1991, Grade: A
HONORS
1. 2012-2013 Technologist Teaching Award presented by Residents of the Dept. of Laboratory Medicine, University of California San Francisco
2. Teaching scholarship to attend California Blood Bank Society annual meeting
3. East West Center M.S. Degree Study Scholarship, 1975-1977. 4. Undergraduate English Scholarship, National Chung-Hsing Univ., 1971-1972
.
APPLICABLE COLLEGE COURSES
Class title Semes. No. of Institution Date
units semes. completed completed
Chemical, Cellular Immunology 4 I Univ.ofCA-Davis March, 1995 Comparative Hematology 2 I
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