Kenneth W. Henry II, Ph.D.

***** ******* ***., #***, *** Diego, CA 92126

760-***-****, aduudg@r.postjobfree.com

Summary

More than twenty years diversified experience, plus a Ph.D., combine to offer a versatile, analytically creative research professional with training and supervisory experience. Background includes considerable expertise in numerous molecular, biochemical, and cell biology techniques. Has successfully managed up to six employees to consistently achieve project goals.

Technical Expertise

•Plasmid Construction

•Mammalian Cell Culture

•Establishing Primary Cell Lines

•Transient Transfections; Recombinant Gene Expression & siRNA Expression Knockdown

•Stable Transfections; Classic & Retrovirus

•Induced Pluripotent Stem Cell Directed Differentiation

•Recombinant Protein Expression and Purification; Bacteria, Yeast & Mammalian Cells

•In Vitro Radioactive Binding Assays

•Flow Cytometry – BD FACS Aria, DIVA 8 Software

•Immunofluorescent labeling

•Confocal Microscopy

•Transmission Electron Microscopy

•Fibroblast Reprogramming to Induced Pluripotent Stem Cells

•Northern, Southern, Western Analysis

•ELISA

•Quantitative RT-PCR

•RNA / DNA Isolation and Manipulation

•Electroporation

•Maintained Animal Breeding Colonies

•Small Animal Surgery

•Animal Behavior Studies (Allodynia, Hyperalgesia

and Motor Coordination)

•Cell Based Assays

Proliferation

Apoptosis / Senescence

Invasion / Migration

Intermediate Signaling Events (i.e. cAMP

[ELISA], Intracellular Ca++ flux [FLiPR]).

Transcriptional Regulation of Synthetic Reporter

Gene

Education

Certificate, University of California, San Diego, La Jolla, CA 2020

Biotechnology Project Management

Ph.D., University of Kentucky, Lexington, KY 2000

Biochemistry

Master of Science, University of Nevada, Reno, Reno, NV 1990

Cell Biology

Bachelor of Arts, University of California, San Diego, La Jolla, CA 1985

Cell Biology and Biochemistry

Experience

Sanford Consortium for Regenerative Medicine, La Jolla, CA November 2020 – Current

Laboratory of Martin Marsala

Staff Research Associate

Reports to Principal Investigator. The focus of this laboratory is developing surgical techniques to introduce stem cell, and viral gene therapies to treat neurological diseases. I was principally responsible for managing and preparing stem cells and viruses for investigative and clinical application. I also functioned as the Lab Manager with respect to reagent ordering, equipment maintenance, and regulatory compliance.

Created plasmid constructs for Adeno Associated Virus (AAV) expression of gene therapies. Results: AAV mediated gene therapies created for spinal cord injection.

Supervised the reception and clinical preparation of a novel stem cell therapy for cerebral injection. Results: Established protocol to determine cell viability following shipment from Japan at 5oC. Helped organize scheduling for efficient receiving and transition to clinical application.

Served as person of contact for important collaborator in Japan, Sumitomo Pharmaceuticals. Results: Funding consistently renewed as numerous collaborative projects moved forward.

Participated in grant writing. Result: National Institute of Health R01 grant funding secured.

Solely responsible for mammalian cell culture activities. Result: Several populations of Neuronal Progenitor Cells differentiated from human induced Pluripotent Stem Cells.

Sanford Consortium for Regenerative Medicine, La Jolla, CA August 2016 – September 2020

Laboratory of Larry Goldstein

Lab Manager

Reported to Principal Investigator. Functioned in a dual role as both central figure in managing all laboratory activities, and as an independent investigator. My particular skills in propagating human embryonic and induced pluripotent stem cells, and their derivative cell lines, and the incorporation of these cells into cell-based assays, has enhanced their role in pursuing novel therapeutics.

Conducted cell-based research in Amyotrophic Lateral Sclerosis (ALS), also known as Lou Gehrig’s Disease, under California Institute for Regenerative Medicine funding. Results: Directly contributed to achieving milestones in the translation of experimental stem cell research to clinical trials.

Individually developed and performed numerous cell-based assays investigating repurposed drug potential in treating Alzheimer’s Disease. Results: Identified several drugs as therapeutic candidates to target specific Alzheimer’s Disease indications.

Developed and conducted numerous differentiation procedures to transform induced human pluripotent stem cells into cortical neuron populations for RNASeq analysis. Results: Important collaborations maintained.

Managed student interns to provide routine maintenance of equipment and supplies. Result: Facilitated all experimentation in a leading biological research laboratory.

Served as point of contact person for all purchases of supplies and equipment. Result: Oversaw the value driven purchasing decisions of thousands of dollars every month.

University of California, San Diego, La Jolla, CA October 2009 – August 2016

Department of Anesthesiology, School of Medicine.

Lab Manager

Reported to Principal Investigator. Functioned as senior lab investigator, performing individual experiments as well as coordinating and/or facilitating all experimentation performed by other members of the lab. This included considerable mentoring and training duties. Brought in new technologies to the lab and established numerous protocols. Was the point of contact person for all safety and regulatory compliance including Environmental, Health and Safety (EH&S) and IACUC regulations.

Maintained knockout mice colonies, performed small animal surgery to evoke pain models, monitored resulting pain behaviors and molecular phenotypic changes; Result: Successfully reported results in peer reviewed journals.

Demonstrated expertise in recombinant signaling peptide expression; Result: Established the lab as a unique collaborator in the study of pain resolving/nerve regeneration peptides.

Successfully established mouse primary Schwann Cell cultures; Result: Allowed for the in vitro study of neuronal cytokine signaling.

Participated in the writing of scientific papers and grant applications; Result: Published scientific papers and grant funding awarded to the lab.

National Center for Microscopic and Imaging Research, San Diego, CA March 2008 – October 2009

Independently run research center on the campus of UCSD.

Staff Research Associate I

Reported to Project Leader. Participated in developing novel microscopic and imaging techniques for application in biological research. Demonstrated expertise in techniques involved in fluorescent and electron microscopy. Duties include materials ordering and maintenance of laboratory equipment.

Acquired technical expertise in photoconversion of fluorescent signal to electron dense transmission electron microscopic labeling; Result: Participated in numerous collaborative projects as technical advisor and trainer.

Established a new protocol for fluorescent biarsenical labeling post aldehyde fixation.

Isolated several homogenous, recombinant protein stably expressing cell lines; Result: Enhanced the ease by which techniques can be applied to 100% recombinant protein expressing cell lines.

Trained visiting scientist and collaborators in multiple microscopic techniques; Result: Successful collaborations maintained.

SUNBIOCHEM, San Diego, CA October 2004 - August 2005

Privately-owned company specializing in research / drug development.

Senior Scientist

Reported to the President. Responsible for performing and supervising all compound characterization experiments, usually centering around cell-based assays. Designs, conducts, and reports evaluations for all in-house experimentation. Major contact and organizer of all outside collaborative and outsourced experimentation. Duties include materials ordering and maintenance of occupational safety standards.

Established a high throughput proliferation primary screening program to examine the anti-cancer potential of novel compounds; Result: Promising lead compounds discovered.

Designed advanced characterization procedures to examine the advent of apoptosis in response to novel compounds; Result: Able to demonstrate the induction of apoptosis by our compounds.

Demonstrated knock-down of mammalian cell expression of a GFP reporter gene using full-length anti-sense GFP expression from a retro-virus expression plasmid; Result: This technology validates it is possible to isolate specific gene products involved in physiological distinct responses to novel compounds using anti-sense expression libraries.

SEQUENOM, INC. (formerly Axiom Biotechnologies), San Diego, CA January 2001 - July 2004

Publicly traded genomics technology, research, and drug development company. 250 employees.

Scientist

Reported to the Research Manager. Responsible for working as the leading mammalian cell culture scientist. Oversaw all mammalian cell culture and cell-based assay activities.

Achieved note as the most active and productive developer of cell-based assays to address scientific questions. Developed comprehensive cell-based assay program to examine GPCR mediated, intermediate signaling pathway activity, including intracellular cAMP quantitation (ELISA), intracellular Ca++ flux (FLiPR), and membrane potential changes (Proprietary High Throughput Pharmacology System [HT-PS]).

Continued proficient track record of isolating genes important to physiological processes and ailments; Result: Patent filed.

Managed Scientists and Research Associates by generating a team atmosphere; Result: Established a productive, cohesive research group.

Developed significant expertise in the maintenance and manipulation of mammalian cells for use in drug development research by working with in excess of 150 different cell lines.

The Scripps Research Institute, La Jolla, CA March 2000 - January 2001

Largest non-profit research institute in the USA.

Principal Investigator, Post Doctoral Researcher

Reported to Principal Investigator. As a post-doctoral researcher, was responsible for conducting research into the mechanisms of oncogenic progression.

Acquired and demonstrated considerable amount of expertise in the handling of retrovirus.

Developed ability to analyze numerous events in tumor progression.

University of Kentucky Lexington, KY August 1993 - March 2000

Department of Biochemistry, School of Medicine.

Graduate Student

Reported to Principal Investigator. Initiated research facilities and projects from inception. Functioned as lab manager, ordering and maintaining supplies and equipment, training and supervising laboratory personnel.

Developed novel protocols and procedures to identify novel steroid/hormone nuclear receptors co-factors with functional genomic screening. Result: NIH R1 grant funding secured.

Isolated and characterized several new co-factors and genomic elements involved in steroid/hormone nuclear receptor signal transduction; Result: Several scientific papers published.

Mentored graduate students and departmental professors in the use of yeast and mammalian cell-based assays.

S.I.B.I.A., Inc. San Diego, CA November 1992 - August 1993

Company specialized in research and drug development.

Associate Scientist, Cellular and Molecular Biology

Reported to the Director of Research. Responsible for management of mammalian tissue culture facility in conjunction with neurological disease research.

Created numerous Excitatory Amino Acid Receptor stable cell lines.

Trained and managed Research Associates

Ligand Pharmaceuticals, Inc., San Diego, CA June 1990 – November 1992

Publicly traded research and drug development company. Specializes in discovery, development, and marketing new drugs that address critical unmet medical needs of patients. 125 employees.

Research Associate

Reported to the Director of Research. Responsible for cellular and molecular biology research projects.

Conducted diverse experiments to study the function of steroid / hormone nuclear receptors.

Developed transcriptional activation reporter system in yeast.

Demonstrated expertise in recombinant protein expression and protein purification with bacteria and yeast.

University of Nevada, Reno, Reno, NV August 1988 - May 1989

Graduate Teaching Assistant, Department of Biology

Instructor of Genetics, and Anatomy and Physiology courses.

Publications

1. Poplawski, G., Ishikawa, T., Brifault, C., Lee-Kubli, C., Regestam, R., Henry, K.W., Shiga, Y., Kwon, H., Ohtori S., Gonias, S.L. and Campana, W.M. Schwann Cells Regulate Sensory Neuron Gene Expression Before and After Peripheral Nerve Injury. Glia. 2018 Mar 9. doi: 10.1002/glia.23325.

2. Campana W.M., Mantuano, E., Azmoo, P., Henry, K., Banki, M.A., Kim, J.H., Pizzo, D.P., and Gonias, S.L. Ionotropic Glutamate Receptors Activate Cell Signaling in Response to Glutamate In Schwann Cells. FASEB. 31(4): 1744-55, 2017.

3. Flutsch A., Henry, K., Mantuano, E., Lam, M.S., Shibayama, M., Takahasshi, K., Gonias, S.L. and Campana, W.M. Evidence that LDL Receptor-related Protein 1 Acts as An Early Injury Detection Receptor and Activates c-Jun in Schwann Cells. Neuroreport. 27(18): 1305-11, 2016.

4. Lee-Kubli, C.A., Ingves, M., Henry, K.W., Shiao, R., Collyer, E., Tuszynski, M.H., and Campana, W.M. Analysis of the behavioral, cellular and molecular characteristics of pain in severe rodent spinal cord injury. Exp. Neurol. 278: 91-104, 2016.

5. Yamauchi, K., Yamauchi, T., Mantuano, E., Murakami, K., Henry, K., Takahashi, K., and Campana, W.M. Low-Density Lipoprotein Receptor Related protein-1 (LRP1)-Dependent Cell Signaling Promotes Neurotrophic Activity in Embryonic Sensory Neurons. PLoS One 8(9): e75497, 2013.

6. Yoon, C., Van Niekerk, E.A., Henry, K., Ishikawa, T., Orita, S., Tuszynski, M.H., and Campana, W.M. Low-density Lipoprotein Receptor-related Protein1 (LRP1)-dependent cell signaling promotes axonal regeneration. J. Biol. Chem. 288(37): 26557-68, 2013.

7. Orita, S., Henry, K., Mantuano, E., Yamauchi, K., De Corato, A., Ishikawa, T., Feltri, M.L., Wrabetz, L., Gaultier, A., Pollack, M., Ellisman, M., Takahashi, K., Gonias, S.L., and Campana, W.M. Schwann cell LRP1 regulates remak bundle ultrastructure and axonal interactions to prevent neuropathic pain. J. Neurosci. 33(13): 5590-602, 2013.

8. Mantuano, E., Henry, K., Yamauchi, T., Hiramatsu, N., Yamauchi, K., Takahashi, K., Lin, J. H., Gonias, S. L., and Campana, W. M. The Unfolded Protein Response is a Major Mechanism by which LRP1 regulates Schwann Cell Survival After Injury. J. Neurosci. 31(38): 13376-85, 2011.

9. Noonan, D. J., Henry, K., and Twaroski, M. L. A High-throughput Mammalian Cell-based Transient Transfection Assay. Methods Mol. Biol. 284: 51-66, 2004.

10. Koszewski, N. J., Henry K. W. 2nd, Lubert, E. J., Gravette H., and Noonan, D. J. Use of a Modified Yeast One-hybrid Screen to Identify BAF60alpha Interactions with the Vitamin D receptor Heterodimer. J. Steroid Biochem. Mol. Bio. 87(4-5): 223-231, 2003.

11. Henry, K. W. 2nd, Spencer, M.L., Theodosiou, M., Lou, D., and Noonan D.J. A Neuronal-specific Differentation Protein that Directly Modulates Retinoid Receptor Transcriptional Activation. Nuclear Receptor. 1:7-16, 2003.

12. Henry, K.W. 2nd, Carey, B., Howard, W. R., Hoefner, D., and Noonan, D.J. Use of Saccharomyces cerevisiae in the Identification of Novel Transcription Factor DNA Binding Specifics. Yeast. 18: 445-454, 2001.

13. Henry, K.W., Yuan, X., Koszewski, N.J., Onda, H., Kwaitkowski, D.J., and Noonan D.J. Tuberous Sclerosis Gene 2 Product Modulates Transcription Mediated by Steroid Hormone Receptor Family Members. J. Biol. Chem. 273(32): 205**-*****, 1998.

14. O’Brien, J.L., Rangwala, S., Henry, K.W., Weinberger, C., Crick, D.C., Waechter, C.J., Feller, D. R., and Noonan, D.J. Convergence of Three Steroid Receptor Pathways in the Mediation of Nongenotoxic Hepatocarcinogenesis. Carcinogenesis. 17: 185-190, 1996.

15. Henry, K.W. 2nd, O’Brien, M.L., Clevenger, W., Jow, L., and Noonan, D.J., Peroxisome Proliferator-activated Receptor Response Specificities as Defined in Yeast and Mammalian Cell Transcription Assays. Toxicol.Appl.Pharmacol. 132: 317-324, 1995.

16. Henry, K.W. 2nd, Brown, J.L., and McCracken, A.A. Selective Enrichment for Temperature-sensitive Secretion Mutants of Mammalian Cells Using Plant Lectin, Concanavalin A. Somat.Cell Mol. Genet. 16(4): 297-304, 1990.

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