TAMERA M. FRANKS

**** **** **. ***.***

College Station TX, 77845

Cell: 770-***-****

adu0co@r.postjobfree.com

LABORATORY SKILLS AND TECHNIQUES

●Laboratory Management 6 years (PITT)

●BSL2+ proficient 6 years (PITT)

●PCR 6 years (PITT)

●Virus engineering

●ABI 3100 DNA sequencer system

●Flow cytometry 6 years (PITT)

●Western blotting

●Lipofectamine-based transfection

●HPLC 6 years (PITT)

●Tissue Culture 6 years (PITT) Years (EMORY)

●Fluidigm

●Site directed mutagenesis

●SOLO liquid handling robot (Pipettor and Dispenser: Serial Dilutions)

●Luciferase-based assays

●Virus titering 6 years (PITT)

●Molecular cloning

●Sequencing analysis

●Capable of performing P24 & P27 ELISA assays 6 years (PITT)

●Microbiology 10 + Years (PITT, CDC, EMORY)

●Biohazard handling and disposal 10 + Years (PITT, CDC, EMORY)

●MicroFlo

●TECAN: Freedom EVO 200

●WellPro 3000

●King Fisher Flex DNA Extractor

●Affymetrix microarray assay

●Leukapheresis processing 10+ Years (PITT, EMORY)

PROFESSIONAL EXPERIENCE

MDx BioAnalytical Laboratory, Inc

General Supervisor/Clinical Biologist January 2022-Oct 2022

Managed employee training for COVID TESTING

Offering continuous guidance and mentorship on best practices while offering constructive feedback

Used both rapid as well as RT-PCR for high throughput testing

Maintained records for CLIA certification

The Hope Clinic of Emory University, Atlanta GA March 2015-Nov 2018

Research Specialist, Lead: The Hope Clinic of Emory University

●Provide critical feedback to optimize the Laboratory Information Management System used for Center for AIDS Research processing procedures

●Train new and visiting staff on all clinical and research procedures

●Ensure compliance with policies, such as biosafety, through education and awareness

●Cultivate strategies to improve efficiencies within the lab and reduce errors

●Perform protocol quality checks at the end of each month resulting in excellent audits for three years

●Coordinate, process, ship, and quality assure for two major sponsors supported by a grant worth $135 million

●Develop and maintain specimen inventory logs and report data to management

●Master assays and optimize according to research goals to ensure collection of clear and consistent data

●Assist with sample processing and quality control in times of high volume

●Attend seminars and journal clubs within Emory University to keep up-to-date in the field

Emory University, Atlanta, GA April 2014 – March 2015

Research Specialist: Emory Integrated Genomics Core

●Preform DNA/RNA extractions from blood and various tissues.

●Quantitative and qualitative analysis using BioAnalzyer and NanoDrop technology.

●Preform Methylation and Gene Expression Microarrays on the llumina HiScan

●RNA and DNA analysis by utilization of Affymetrix microarray assay

●DNA sequencing and data analysis

●Performed various tests on samples to decide how to properly dispose of truck load, tested the samples for the following: acid content, flashpoint, flammability, viscosity, pH, and trace elements.

●Recorded data daily into numerous sample log books, based on test used and EPA quality control verifications.

●Sample re-sequencing utilizing the Access Array System from Fluidigm

Centers For Disease Control, Atlanta, GA July 2013-April 2014

Microbiologist: Picornaviridae unit

●Maintained cell lines for the infection of poliovirus.

●Processed stool samples in order to test for poliovirus

●Monitored infected cell lines for poliovirus outbreak.

University of Pittsburgh, Pittsburgh, PA Jan 2008 – June 2013

Laboratory Manager/Laboratory Technician III, Dept. Of Medicine

●Responsible for the daily supervision of lab personnel, which includes the training of newly hired lab personnel, assigning job duties, and weekly planning of the lab schedule.

●Developed an inventory tracking system, which is presently used in the logging and tracking of a high number of lab chemicals, media, assay kits, and other consumables.

●Solely accountable for the tracking, monitoring, and spending of research grant monies, including: RO1, NIH, NCI, amFAR, P50 PCHPI, Merck, and CMRF.

●Order products as needed for general laboratory stock and laboratory machinery to complete ongoing experiments.

●Calibrates, and performs routine repairs on lab equipment.

●Maintaining various cell lines, CEM, 293T, HeLa, GHOST, TZMs, HUT, Macrophages, and Jurkat cells.

●HIV viral load testing of primate (Rhesus Macaque) blood samples.

●Coordinating meetings with various companies’ representatives to obtain information about laboratory products and/or instrumentation for potential purchases.

●Knowledgeable in the receiving, handling, storing and shipping of hazardous or potentially dangerous agents.

Duquesne University. Pittsburgh, PA Sept 2006-Dec 2007

Clinical Research Technician, Dept. Of Pharmacology

●Performed animal/memory observational studies of rodent behavior.

●Recorded data, created charts and graphs of observed data, and presented it to PI bi-weekly, for later use in publications or presentations.

●Performed animal neurological dissections.

●Conducted surgical procedures on rodents, the implantation and/or removal of hormonal capsules.

●Oversaw the daily care of study animals, which included feedings and taking their weights.

COMPUTER SKILLS

- Excel, Access, Word, PowerPoint, Mac OS

- CITI GCP - LIMS database

- CITI Human Subjects Protection - LDMS database

- DMID Regulatory Training - DNASTAR: Lasergene

- IATA Shipping

EDUCATIONAL BACKGROUND

Rosemont College, Rosemont, PA May 2004

Bachelor’s of Science. Biology/English Literature

(Concentration in Microbiology and Genetics)

Publications

Evidence for biphasic uncoating during HIV-1 infection from a novel imaging assay (Link)

Retrovirology July 9, 2013

Persistence of Viral Reservoirs in Multiple Tissues after Antiretroviral Therapy Suppression in a Macaque RT-SHIV Model.(Link)

PLoS One. December 18, 2013

• Specific References & Supporting Documentation Furnished Upon Request •

Contact this candidate