CURRICULUM VITAE

ASA F. GARDNER, JR.

**** ********** *** ***, **** Robles, CA 93446

Phone: 415-***-****

adt9wk@r.postjobfree.com

EDUCATION

****-** ****** (n progress), Lake Erie College of Osteopathic Medicine, Erie, PA

2014-18 M.S. in Molecular and Systems Biology, University of California, Merced, CA

Biomedical sciences, molecular biology, and cancer biology

2010-13 ABD in Neuropharmacology/Molecular Biology, Ohio State University, Columbus, OH

Pharmacology, molecular, cell biology, electrophysiology, and cloning

2005-08 B.S. in Physiology and Neuroscience, University of California, San Diego, CA

Certifications: CGMBS Clinical Molecular Biological Scientist Trainee License, & Intern Pharmacist License

RESEARCH & PROFESSIONAL EXPERIENCE

08/20 – Adjunct Faculty, Hartnell College, Salinas, CA

Present Teaching: Intro. Biology, Anatomy and Physiology, Microbiology, Cell Biology, Human Biology

Lecture of classes at Hartnell as well as Hancock college concurrently.

Designing of lectures, multiple times a week, writing and grading of exams, run and grade labs, writing the syllabus while abiding by government sanctioned rules for the class, and held three office hours a week

Sought out and attended professional development seminars and classes to fine tune my teaching acumen

Ability to teach a 100% online course and stick to all the necessary guidelines as well as keep the class active outside of the classroom.

Multitude of experience working with; socio-economically disadvantage population, the Latinx population, 1st generation college students and an understanding of these and many other minority groups.

01/19 – 06/22 Adjunct Faculty, Allan Hancock College, Santa Maria and Lompoc, CA

Teaching Bio 100; Introductory Biology and Bio 124; Human Anatomy

Designing of lectures, lecturing multiple times a week, writing and grading of exams, run and grade labs, writing the syllabus while abiding by government sanctioned rules for the class, and held three office hours a week

Sought out and attended professional development seminars and classes to fine tune my teaching acumen

Ability to teach a 100% online course and stick to all of the necessary guidelines and maintain

06/21 – 01/22 Intern Pharmacist, Rite-Aid, CA

Skills: Patient counseling, prescription reconciliation, filling Rx, making recommendations

Patient counseling, recommendations, and medical management

Vaccinations, and immunizations

Entering, filling, and quality checking of prescription medication Expert

01/18 – 12/18 Senior Graduate Research Associate, University of California, Merced – Dr. Joel Spencer

Skills: Animal work, hematology, immunology, assay development, SOPs, cell biology, imaging

Mouse irradiation, HSC transplantation, perfusions, surgeries, and animal husbandry

Whole mount staining of the thymus with fluorescent antibodies, tissue clearing techniques, confocal microscopy, and two-photon microscopy, immunological assays, confocal microscopy, pharmacological

Pharmacokinetics and pharmacodynamics, hematology, tissue clearing techniques.

Responsible for the training of all graduate and undergraduate students in lab, ordering and writing SOPs for the lab

05/14-12/17 Senior Graduate Research Associate, University of California, Merced – Dr. Fabian Filipp

Skills: Cancer metabolism, molecular biology, metabolomics, cell culture, proteomics

Design, cloning and use of CRISPR constructs for knockouts, overexpression, genome editing, cell culture maintenance, and viral delivery of constructs

Site-directed mutagenesis, protein expression and purification to determine how enzyme kinetics affect metabolism, transcriptomics, proteomics and NGS.

Characterizing genes using cell proliferation assays, cell viability assays, Western blots, and qPCR techniques.

Immunofluorescence, flow cytometry, cell sorting and counting

HPLC, column chromatography, affinity chromatography and, mass spectrometry

Protein purification using bead chemistry

09/10-08/13 Senior Research Associate, Ohio State University, Columbus, OH – Dr. Chen Gu

Skills: Culture, animal work, molecular, protein interactions, chemistry, editing, pharmacology

Design, cloning and use of a PCR based model of vector cloning, site directed mutagenesis, over expression, genome editing, transfections, and NGS

Site-directed mutagenesis, protein expression, protein purification, protein-protein interaction assays, bead chemistry

Developed a hippocampal neuron culture that could be myelinated and last 35 days for pharmacological experiments to better understand Alzheimer’s Disease, Multiple Sclerosis, and Parkinson’s Disease

Pharmacokinetics and pharmacodynamics

Rat neuron culture, oligodendrocyte culture, organ imaging and testing

12/08-08/10 Research Associate II, Salk Institute for Biological Studies – Dr. Greg Lemke

Skills: Western blot, molecular, protein work, surgery, animal culture, neuroscience, histology

Developed protein expression system for the isolation of recombinant Gas6 and Protein S ligands including design of expression constructs, identification of suitable purification techniques, and functional assay to verify ligand activity

Developed protocols, immunohistochemistry, recombinant DNA techniques, animal

primer design, paraffin sectioning, cryo-sectioning, cell culture, protein analysis and purification, bead chemistry, Western blot, histology, design, and facilitation of experimental models, responsible for educating summer intern, and antibody development.

Immunofluorescence, flow cytometry, cell sorting and counting

HPLC, column chromatography, affinity chromatography, and mass spectrometry

Mouse colony breeding, maintenance, weening, blood collection, survival surgery, sexing, dosing, ADME, and triple knock out mice lines

08/05-12/08 Lab Technician/Research Associate I - University of California, San Diego, - Dr.

Anirvan Ghosh

Skills: Molecular biology, neuroscience, pharmacology, histology, cloning, DNA/protein isolation

Mouse perfusions, tissue cultures, brain sectioning, immuno-fluorescent and DAB staining techniques, neuronal imaging, genetic screening of mouse lines, slide preparation, UV

microscopy, neuro-anatomical staining to visualize expression patterns, molecular cloning, PCR, DNA purifications, Western blots, SDS-PAGE, RNA/DNA isolation, transfections, reverse transcriptase procedures, QPCR, electrophysiology experiments, use of immortalized cell lines, recombinant DNA techniques, biochemical assays, prepared common lab solutions, maintained lab stock of chemicals, lab ware, antibodies, and other supplies

Animal husbandry, genotyping, sacrificing and surgeries, breeding weaning, dosing, survival surgery, E18, rat neuron culture, dissection and perfusion

08/04-08/05 Lab Technician, Santa Cruz Biotechnology, Inc., Paso Robles, CA

Skills: Peptide antibody production, purification and validation team

Prepared buffers needed for protein purification, separated proteins from sera using salt precipitation techniques, prepared assays for chromatography, prepared conjugations for immunization boosts, ELISA coating and testing, and protein purification

HPLC, column chromatography, affinity chromatography, bead chemistry

RESEARCH PROJECTS

Visualizing hematopoietic stem cell differentiation in bone marrow to T-cell maturation in the thymus

Working under Dr. Joel Spencer, using a tissue clearing technique I was able to image organs without having to section them as with previous methods. Irradiating mice and then transplanting fluorescent T-cell progenitor cells is a technique in which I then replace progenitor cells in the bone marrow to look at immune cell differentiation. Confocal microscopy then allows the capturing of 3-D images of the whole organ fully intact with the significant decrease of background noise.

Using CRISPR technology for validating possible oncologic targets in metastatic melanoma.

Working under Dr. Fabian Filipp, using biochemical, molecular biology and system biology techniques one is able to identify possible mutations that lead to cancer progression in melanoma. With the use of CRISPR-Cas9, cell culture and other molecular biology techniques an edited genome can lead to possible drug targets.

Myelination of rodent hippocampal neurons in culture

Working under Dr. Chen Gu, I developed a protocol that allows the myelination of rodent hippocampal neurons in vitro. Further studies were done using different pharmacological agents to determine what factors cause demyelination in different neurological disease models.

The role of TAM Kinases in ulcerative colitis

Working under Dr. Greg Lemke, we elucidated the role of TAM kinases in the regulation of the innate immune response. We developed protein expression system for the isolation of recombinant Gas6 and Protein S ligands including design of expression constructs, identification of suitable purification techniques, histological techniques and functional assays to verify ligand-receptor activity as well as down-stream effects.

NMDA receptor regulation by TARPS

Working under Dr. Anirvan Ghosh and Dr. Ben Hall, we looked at how NMDA receptors are trafficked to the synaptic cleft, specifically, the NR2A and NR2B subunits. This was done using electrophysiological and immunohistochemical methods.

SKILLS & PROGRAMMING LANGUAGES (ADVANCED PROFICIENCY)

Microsoft 365

Zoom

Microsoft Office Enterprise

Microsoft Excel

Microsoft Word

Microsoft Power Point

Microsoft Outlook

LINUX

PUBLICATIONS

Gardner, A., Jukkola, P., Gu, C.” Myelination of rodent hippocampal neurons in culture.” Nature Protocols. 7, 774–1782 (2012)

POSTERS & PRESENTATIONS: BL UE A RE TEACHING RELATED

2018 Gardner, A. F. and Spencer, J.A., A Method of Tissue Clearing to Identify Cell Types in the Thymus. Presented at the 19th Annual UC Systemwide Bioengineering Symposium, University of California, Riverside (Poster and talk)

2016 Gardner, A. Mastering the Classroom with 1st Generation College Students. Presented at University of California, Merced - Teaching Matters Workshops, Merced, CA (Oral).

2015 Gardner, A. Developing Teaching Strategies. Presented at University of California, Merced - Teaching Matters Workshops, Merced, CA (Oral).

2015 Gardner, A. Improving Teaching by Assessing Learning. Presented at University of California, Merced - Teaching Matters Workshops, Merced, CA (Oral).

2015 Gardner, A. Assessing Learning in Bio 110 (Cell Biology). Presented at University of California, Merced – Undergraduate Learning Outcomes Assessment: Pedagogy and Program Planning

2012 Gardner, A. and Gu, C., Myelination of Rodent Hippocampal Neurons in Culture. Presented at the Ohio State University Medical Center Research Day, Columbus, OH (Poster).

AWARDS, HONORS & FELLOWSHIPS

2020 Reading Apprenticeship in STEM Fields – California Community Colleges/California State Universities

2017 Quantitative and Systems Biology Summer Fellowship, University of California, Merced, CA 2014-17 Certificate of Proficiency in the Scholarship of Teaching and Learning, Center for Research on Teaching Excellence, University of California, Merced, CA (spring)

2015 Dean’s Travel Award to Attend Nanohub NSF Grant Review at University of Illinois, Urbana-Champaign (spring)

2016 Professional Development Travel Award to SACNAS, University of California, Merced (fall)

2016 Professional Development Travel Award to ABRCMS, University of California, Merced (fall)

2015 Assessment as Pedagogy Fellowship, University of California, Merced, CA (fall)

2015 Instructional Internship Program Fellowship, University of California, Merced, CA

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