KHOULOUD BARAKAT * Microbilogy Lab.

Curriculum Vitae

Personal Information:

Name : Khouloud Mohamed Barakat

Title : Professor of Marine Microbiology.

Date/place of Birth : May, 7th 1976, Alexandria, EGYPT. Marital Status : Married, 2 children.

Nationality : Egyptian.

Appointment : Professor of Microbiology.

Work Address : National Institute of Oceanography and Fisheries, Al-Anfoushi, Qayed Bay, Alexandria, Egypt.

Home Address : 36, Tout Ankh Amoun Street, Alexandria, Egypt. Language : Arabic, English & French

Tel. [Work] : +2-03-480**** – 4807140

Tel. [Residence] : +203*******

Mobile : +201********* - +201*********

E-mail : adt883@r.postjobfree.com and adt883@r.postjobfree.com Academic Qualifications:

1. Ph.D. of Microbiology - Botany department, Microbiology section, Faculty of Science, Alexandria University, Egypt in 2008

Thesis title "Microbial production and evaluation of biologically active agents against some bacterial fish pathogens.

2. M.Sc. of Microbiology - Botany department, Microbiology section, Faculty of Science, Alexandria University, Egypt in 2003

Thesis title of "Degradation of triphenylmethane by free and immobilized Bacteria isolated from sea water"

3. Postgraduate courses for M. Sc. Degree in microbiology with excellent (1999 – 2000) - Botany department, Microbiology section, Faculty of Science, Alexandria University, Egypt: (Special Microbiology – Special courses- Fungal soil - Bacterial soil -Enzymology - Biostatistics - Computer - German language).

4. B.Sc. In Microbiology with very Good with honor, Botany department, Microbiology section, Faculty of Science, Alexandria University, Egypt. in May 1998 Professional Qualifications:

Head of Microbiology LAB from November 2020 to November 2021 1- Professor (Dec. 2019) Marine Environment Division, Microbiology Lab., National Institute of Oceanography and Fisheries, Alexandria, Ministry of Scientific Researches, Egypt.

2- Associated Professor (Feb. 2014) Marine Environment Division, Microbiology Lab., National Institute of Oceanography and Fisheries, Alexandria, Ministry of Scientific Researches, Egypt.

3- Assistant professor (2010-2012) Faculty of Sciences and Humanity studies, Shaqra University, Saudi Arabia

Dr. Khouloud M. BARAKAT

Microbiology Lab,

Marine Environment Division,

National Institute of Oceanography and Fisheries

Anfoushy, P. O Alexandria, EGYPT

KHOULOUD BARAKAT 2 Microbilogy Lab.

4- Researcher (2008- 2014) Marine Environment Division, Microbiology Lab., National Institute of Oceanography and Fisheries, Alexandria, Ministry of Scientific Researches, Egypt.

5- Assistant Researcher (2003-2008) Marine Environment Division, Microbiology Lab., National Institute of Oceanography and Fisheries, Alexandria, Ministry of Scientific Researches, Egypt.

6- Researcher Assistant (1999-2003) Marine Environment Division, Microbiology Lab., National Institute of Oceanography and Fisheries, Alexandria, Ministry of Scientific Researches, Egypt.

Teaching Experience:

1. General Microbiology courses.

2. Bacteriology.

3. Pollution.

4. Scientific Added Value Expert.

Scientific Fields of Interest:

1. Isolation, characterization and biotechnological studies on marine bacteria, yeast and fungi 2. Focusing on the marine bacterial resources in different habitats. 3. Antibiotic resistance in bacteria.

4. Antibiotic structure-activity relationships.

5. Marine Natural products and antibiotic synergism. 6. Fish Microbiology

7. Chemotherapy for fish pathogenicity.

Publications

1. Manal, M. A. EI-Naggar; Samy, A. EI-Aasar and Khouloud, I. Barakat. (2004). Decolorization of different groups of dyes by free" and immobilized cells of a marine Pseudomonas aeruginosa. BULL. FAC. SC. ASSIUT UNIV. (33): 173-181. 2. ManaI, M. A. EI-Naggar; Samy, A. EI-Aasar and Khouloud, I. Barakat. (2004). Bioremediation of crystal violet using air bubble bioreactor packed with Pseudomonas aeruginosa. Water Research Vol. 38, 4313-4322. 3. Manal M. A. El-Naggar, Saffinaz Gomaa, Yousry M. Gohar, Khouloud I. Barakat and Magdy K. Soliman (2007): Bio-control of some fish pathogens using marine Burkholderia cepacia by-product. (MEDCOAST 2007 Internationa l Conference), 659-670.

4. Khouloud M. Barakat and Hermine Tadros (2009) "In Vitro Antibacterial Activity from Marine Algal Extracts against Virulent Bacteria". Bulletin of Higher Institute of Public Health. Vol. 39(3), pp. 432-448.

5. Manal M. A. El-Naggar and Khouloud M. I. Barakat (2009). Antipneumonial agent(s) from marine bacteria. Journal of Natural Products, Vol. (2), pp. 40-48. 6. Manal El-Naggar, Tarek Osman, Amr Mounir and Khouloud M. Barakat (2009).

"Integrated system for rearing Mugil species in a crude oil treated seawater using a marine Pseudomonas aeruginosa strain elnaggar1". African Journal of Microbiology Research, Vol. 3(12), pp. 910-920. (Impact factor 0.407). KHOULOUD BARAKAT 3 Microbilogy Lab.

7. Yousry M. Gohar, Manal M. A. El-Naggar, Magdy K. Soliman and Khouloud Barakat (2010) Characterization of marine Burkholderia cepacia antibacterial agents. Journal of Natural Products, 3: 86-94.

8. Khouloud M. Barakat and Yousry M. Gohar, (2012) "Antimicrobial Agents Produced by Marine Aspergillus terreus var. africanus Against Some Virulent Fish Pathogens" Indian Journal of Microbiology, Vol. 52, pp. 366-372.(Impact factor 0.511- Springer).

9. Khouloud M. Barakat,(2013) "Decolorization of two azo dyes using marine Lysobacter sp. T312D9" Malaysian Journal of Microbiology, Vol. 9(1), pp. 93-102. 10. Rania M. Abedin and Khouloud M. Barakat, (2013) "Evaluation of medium components using Plackett-Burman design for phenol degradation by marine degrading Fusarium oxysporum. African Journal of Microbiology Researches, Vol. 7(7), pp. 541-550. (Impact factor 0.564l).

11. Yousry M. Gohar, Khouloud M. Barakat, Moursy A. Abu Yousef, and Hasnaa B. Ghonam (2013). "Marine Chitosan-Silver Nano-Particles as Antibiotic Synergizers against Diabetic Foot Bacteria". Archives of Clinical Microbiology, 4(2), 1-8. 12. Yousry M. Gohar and Khouloud M. Barakat (2013). "Isolation of a New Non- Toxic Pyrrolidine Derivative with Anti-MDR Bacteria from a Marine Isolate of Aspergillus flavipes", Assuit University, Journal of Botany, Vol. 42(1), pp. 1-13. 13. Hermine Ramzy Zaki Tadros, Mona Fathy El Naggar, Khouloud M. I. Barakat, Amaal Eid Abbas Abou-Taleb & Fatma Abd El Rahman Zaghloul. (2015) Impact of Some Physicochemical and Biological Factors on Steel Corrosion in Seawater Asian J. Adv. Basic Sci, 3(2), 64-73

14. Khouloud M Barakat and Yousri M Gohar (2015) Nanosilver-marine fungal chitosan as antibiotic synergizers against sepsis fish bacteria. Iranian Journal of Microbiology, 7 (6): 273-280.

15. Khouloud M. Barakat and Ehab A. Beltagy (2015) "Bioactive phthalate from marine Streptomyces ruber EKH2 against virulent fish pathogens” Egyptian Journal of Aquatic Research, Vol. 41(1), pp 49–56. (Elsevier). 16. Khouloud M Barakat, Magdy Z. Mattar, Shawky Z Sabae, Osama M Darwesh and Sahar H Hassan (2015) Production and Characterization of Bioactive Pyocyanin Pigment by Marine Pseudomonas aeruginosa OSh1. Research Journal of Pharmaceutical, Biological and Chemical Sciences 6(5): 933-943. 17. Aida H. Shobier, Safaa A. Abdel Ghani, Khouloud M. Barakat (2016) GC/MS spectroscopic approach and antifungal potential of bioactive constituents produced by marine macroalgae. Egyptian Journal of Aquatic Research. 42, 289–299.

(Elsevier)

18. Khouloud M. Barakat and Mohamed Saleh (2016) Bioactive Betulin produced by marine Paecilomyces WE3-F. Journal of Applied Pharmaceutical Science, 6(3): 34- 40.

KHOULOUD BARAKAT 4 Microbilogy Lab.

19. Khouloud M. Barakat, Heba S. El-Sayed, Yousry M. Gohar (2016) Protective effect of squilla chitosan - silver nanoparticles for Dicentrarchus labrax larvae infected with Vibrio anguillarum. International Aquatic Research, 8(2): 179-189. (Springer).

20. Moustafa Y. El-Naggar, Khouloud M. Barakat, Nowara S. Aly (2016) Physiological response, antibacterial activity, and cinnamaldehyde production by a marine Streptomyces chartreusis. Journal of Pure and Applied Microbiology, Vol. 10(3), p. 1797-1808.

21. Heba S. El-Sayed and Khouloud M. Barakat (2016) Effect of dietary chitosan on challenged Dicentrarchus labrax post larvae with Aeromonas hydrophila. Russian Journal of Marine Biology, Vol. 42, No. 6, pp. 501–508. (Impact factor 0.407). 22. El-Bermawi N., Essa M A., El-Sayed H.S., Khouloud M. Barakat and Fadel K. A.

(2016). Green water promotes survival, growth, metabolic composition and water quality in early larval stages of European sea bass (Dicentrarchus Labrax). Mediterranean Aquaculture Journal (8); 95-106.

23. Khouloud M. Barakat, E.H. Zaghloul, A.M. Kasem, S.A. Sabry, E.R. El-Helow. Enhancement of anticoagulant properties of seaweeds by fermentation using marine lactic acid bacteria J Biotechnol Biomater 2016, 6: 6 (Suppl), 2nd World Congress on Bio Summit & Molecular Biology Expo "October 10-12, 2016 Dubai, UAE" http://dx.doi.org/10.4172/2155-952X.C1.061.

24. Khouloud M. Barakat, Sahar W. Mostafa, Osama M. Darwesh (2017). Biosurfactant production by haloalkaliphilic Bacillus strains isolated from Red Sea, Egypt. Egyptian Journal of Aquatic Research Egypt Volume 43, Issue 3, September, Pages 205-211. (Elsevier).

25. Khouloud M. Barakat (2017). Mycodegradation of Crude Petroleum Oil by Locally Marine Candida tropicalis. Microbiology Research Journal International 21(6): 1-10. 26. Sahar M.W. Hassan, Khouloud M. Barakat., and Hassan A.H. Ibrahim (2018). Enhancement of lipid accumulation in oleaginous marine yeast; Candida sp. for biodiesel production and antimicrobial properties. Egyptian Journal of Aquatic Biology & Fisheries 22(4): 195- 207.

27. Heba H. Abdel-Mohsen, Elham A. Wassef1, Nagy M. El-Bermawy, Nabila E. Abdel-Meguid, Norhan E. Saleh, Khouloud M. Barakat, Omayma E. Shaltout

“Advantageous effects of dietary butyrate on growth, immunity response, intestinal microbiota and histomorphology of European Seabass (Dicentrarchus labrax) fry. Egyptian Journal of Aquatic Biology & Fisheries 22(4): 93- 110 (2018). 28. Elham A. Wassef, Norhan E. Saleh, Nabila E. Abdel-Meguid, Khouloud M. Barakat, Heba H. Abdel-Mohsen1 & Nagi M. El-bermawy (2019). Sodium propionate as a dietary acidifier for European seabass (Dicentrarchus labrax) fry: immune competence, gut microbiome, and intestinal histology benefits. Aquaculture International 27 (144):1–17 (Springer - Impact factor 1.455). KHOULOUD BARAKAT 5 Microbilogy Lab.

29. Mohamed S. Amer, Khouloud M. Barakat, Abd-Elatif A. Hassanein (2019). Phthalate derivatives from marine Penicillium decumbens and its synergetic effect against sepsis bacteria. Biointerface Research in Applied Chemistry. 9(4): 4070 – 4076.

30. Osama M Darwesh, Khouloud M Barakat, Magdy Z Mattar, Shawky Z Sabae, Sahar H Hassan (2019). Production of antimicrobial blue green pigment Pyocyanin by marine Pseudomonas aeruginosa. Biointerface Research in Applied Chemistry. 9(5): 4334 – 4339.

31. Dina S Abdelsameea, Khouloud M. Barakat, Sobhy El-Sayed Hassab El-Nabi, Khaled Mohammed Geba (2019) Isolation, identification, and tissue distribution of the tetraodotoxin- producing bacteria from the Silver-cheeked toadfish Lagocephalus scleratus. Conference: BIONATS: 6th Euro-Mediterranean Conference and Expo- Lifa Sciences, Pharma & Biomedicine Convention Centre of Cairo University, Cairo, Egypt

32. Norhan E. Saleh, Elham A. Wassef, Nagi M. El-bermawy, Khouloud M. Barakat, Heba H. Abdel-Mohsen (2020). Bioconversion of fish-wastes biomass into a fish meal alternate for European seabass (Dicentrarchus labrax) diets. International Aquatic Research. 12:40-52.

33. Mohamed S. Gaballah, Ola Abdelwahab, Khouloud M. Barakat, Dominic Aboagye

(2020). A novel horizontal subsurface flow constructed wetland planted with Typha angustifolia for treatment of polluted water. Environmental Science and Pollution Research. 27:28449–28462 doi: 10.1007/s11356-020-08669-5. Online ahead of print. (Springer - Impact factor 2.76)

34. Moustafa Y. El-Naggar, Khouloud M. Barakat, Mohamed A. Aly-Eldeen, Hasnaa B. Ghoneim and Amira M. Hamdan (2021) Veratraldehyde as a food additive produced by the marine isolate Streptomyces diastaticus LC360811. Egyptian Journal of Aquatic Biology & Fisheries Vol. 25(2): 1 – 19 ISSN 1110 – 6131. 35. Khouloud M. Barakat, Heba S. El-Sayed, Hanan M. Khairya, Mohamed A. El- Sheikh, Sarah A. Al-Rashed, Ibrahim A. Arif, Mostafa E. Elshobary. (2021) Effects of ocean acidification on the growth and biochemical composition of a green alga

(Ulva fasciata) and its associated microbiota to the ocean acidification. Saudi Journal of Biological Sciences. Available online 20 May 2021. In Press, Journal Pre-proof

(Impact Factor 2.802).

36. Ola Abdelwahab, Mohamed S. Gaballah, Khouloud M. Barakat, Dominic Aboagye

(2021) Pilot modified settling techniques as a novel route for treating water effluent from Lake-Marriott. Journal of Water Process Engineering 42, Pages 101985

(Impact Factor 3.465).

37. Hasnaa E-B. Ghonam, Morsy A. Abu Yousef, Yousry M. Gohar, Rafa Almeer, Khouloud M. Barakat. (2021) A new antidiabetic foot bacteria formula from marine chitosan nanosilver-metal complex. Environmental Science and Pollution Research. (Impact Factor 4.223)

KHOULOUD BARAKAT 6 Microbilogy Lab.

38. Osama M. Darwesh, Mohamed S. Mahmoud, Khouloud M. Barakat, Ayman Abuellil, Maged E. Ahmad (2021). Improving the bioremediation technology of contaminated wastewater using biosurfactants produced by novel bacillus isolates. Heliyon 7 (12): e08616 (Impact Factor 2.85)

39. Mohamed S. Gaballah, Ola Abdelwahab, Khouloud M. Barakat, Alexandros I. Stefanakis A pilot system integrating a settling technique and a horizontal subsurface flow constructed wetland for the treatment of polluted lake water Chemosphere 295

(2022) 133844. (Impact Factor 7.086).

40. Ehab A. Beltagy Ahmed Abouelwafa, Khouloud M.Barakat. Bioethanol production from immobilized amylase produced by marine Aspergillus flavus AUMC10636. The Egyptian Journal of Aquatic Research (2022). Volume 48, Issue 4, 325-331. 41. Heba S. El-Sayed, Mostafa E. Elshobary, Khouloud M. Barakat, Hanan M. Khairy, Mohamed A. El-Sheikh, Raymond Czaja, Bassem Allam, Hoda H. Senousy. (2022) Ocean acidification induced changes in Ulva fasciata biochemistry may improve Dicentrarchus labrax aquaculture via enhanced antimicrobial activity. Aquaculture, 560: 738474. (Impact Factor: 4.242).

42. Khouloud M. Barakat, Ahmed Elsayed Abouelwafa, Heba Saad El-Sayed, Ehab Beltagy. (2022) Silver Nanoparticles mediated-marine Fungal Amylase improving Dicentrarchus labrax larvae growth performance. Egyptian Journal of Aquatic Biology and Fisheries. 26(3):817-831.

43. Heba S. El-Sayed, Khaled A. Fadel, Nagy El-Bermawi, Zeinab A. El-Greisy, Omayma E. Shaltout, Samia S. Abouelkheir, Khouloud M Barakat. Effect of Magnetized Water to Improve Growth Performance, Composition Factors and Microbiota of Deteriorated Sea Bass Larvae (Dicentrarchus labrax) Egyptian Journal of Aquatic Biology and Fisheries. 26(4):937-953. 44. Khouloud M. Barakat, Mona M. Ismaiel, Hala E. Abou El Hassayeb, Nermeen A. El Sersy, Mostafa E. Elshobary (2022) Chemical characterization and biological activities of ulvan extracted from Ulva fasciata (Chlorophyta). Rendiconti Lincei. 33, 829–841

Thesis supervision:

I. Ph.D. Supervisions:

PhD. Thesis under the title of “Characterization of marine lactic acid bacteria and studying their potential to ferment marine Biomass for enhanced anticoagulant activity" Botany and Microbiology Department, Faculty of Science, Alexandria University, 2015.

PhD. Thesis under the title of "Isolation and Characterization of the Metabolite(s) from Marine Actinomycetes" Botany and Microbiology Department, Faculty of Science, Alexandria University, 2015.

PhD. Thesis under the title of "Molecular detection of medically important bacteria isolated from Alexandria seawater coastline”. Medical Research Center, Alexandria University, 2019.

KHOULOUD BARAKAT 7 Microbilogy Lab.

II. M.Sc. Supervisions:

MSc. Thesis under the title of "Synergy of Marine Chitosans and Metal Complexes as Bacterial Anti-Virulence Chemotherapeutic Agents". Botany and Microbiology Department, Faculty of Science, Alexandria University, 2010.

MSc. Thesis under the title of "New bioactive compounds from marine origin" Botany and Microbiology Department, Faculty of Science, Alexandria University, 2013.

MSc. Thesis under the title of "Molecular and Physiological Studies on Marine Bacteria producing Bioactive Pigment (s)" Botany and Microbiology Department, Faculty of Science, Mounifia University, 2014.

MSc. Thesis under the title of "Molecular studies on tetraodotoxin (TXT) produced by marine Puffer fish" Zoology Department, Faculty of Science, Mounifia University, 2017. Published Books and Chapters

1. Barakat K. M. and Gohar Y. M. Infectious Fish Diseases and Marine Fungal Metabolites, LAP LAMBERT Academic Publishing, Germany, ISBN-13: 978-3-659- 22667-0EAN:978********** Published on: 2012-09-03. 2. Barakat K. M. and El-Sharkawy H. A. Biofuel Production from Marine Resources, LAP LAMBERT Academic Publishing, Germany, ISBN-13: 978-3-659-68034-2-EAN: Published on: 2015-01-28.

3. Barakat K. M., El-Sharkawy H. A., El-Sayed H., Mostafa S.W. and El Zokm G. The Chitin & Chitosan Potentiality. LAP LAMBERT Academic Publishing, Germany, ISBN-13: 978-3-659-55261-8-EAN: Published on: 2018-09-2. 4. Mostafa M. El-Sheekh, Hassan A.H. Ibrahim, Khouloud M. Barakat, Nayrah A. Shaltout, Waleed M.M. EL Sayed, Reda A.I. Abou-Shanab and Michael J. Sadowsky. 2022. Chapter 7: Potential of Marine Biota and Bio-waste Materials as Feedstock for Biofuel Production: WASTE MANAGEMENT, Opportunities and Challenges for Sustainable Development. CRC Press, USA, Taylor & Francis Group, LLC, pp:123-139. 5. Khouloud M. Barakat and Osama M. Darwesh, 2022. Chapter 2, Marine Microbe Surfactants, Future Implementations. Book Marine Surfactants Edition1st Edition, Imprint CRC Press Pages 48 eBook ISBN9781003307464 Membership of the Editorial Board in Scientific Journals

International Journal of Scientific and Technology Research

International Archive of Medicine

Journal of Transdisciplinary Biomedicine

International Journal of Pollution Research

Biotechnology Journal International

Nano and Medical Materials

Arbitration Scientific Researches

1- Research title: "Effect of Penicillium species on seedling growth of wheat." International Research Journal of Agricultural Science (IRJAS) (ISSN: 2251-0044) KHOULOUD BARAKAT 8 Microbilogy Lab.

2- Research Title: "Phylogenetic diversity and antimicrobial activity of marine bacteria associated with the soft coral Sarcophyton glaucum." Journal of Basic Microbiology (Wiley- VCH)

3- Research Title: "Biodegradation of Methyl Red by Bacillus sp. strain UN2: decolorization capacity, metabolites characterization and enzyme analysis." Environmental Science and Pollution Research (Springer Link).

4- Research Title: "Biochemical Analysis of Blue Mint Bush Essential Oil and Its Antimicrobial Activity against Foodborne Bacteria" HERALD INTERNATIONAL RESEARCH JOURNALS

5- Research Title: "Modulation of Antibiotic Resistance in Pseudomonas aeruginosa by ZnO Nanoparticles." Iranian Journal of Microbiology.

6- Research Title: Chitosan-Silver nanocomposite in Goldfish aquaria: New prospective for Lernaea Cyprinacea control" Journal of Fish Diseases. 7- Research Title: Natural marine bacteria as model organisms for the hazard assessment of consumer products containing silver nanoparticles. Journal of Hazardous Materials 8- Research Title: “Actinobacterial bioactive compounds as a paramount source of biologically important enzyme inhibitors - “A boon to Mankind". Current Bioactive Compounds 9- Research Title: “The Effects of Chitosan and Chitosan Nanoparticles on Water Quality, Growth Performance, Survival rate and Meat Quality of the African Catfish, Clarias gariepinus’. Aquaculture Nutrition

10- Research Title: “Antimicrobial potential of newly isolated Aspergillus terreus MK-1: An approach towards new antibiotics Pharmaceutical Biology 11- Research Title: In vitro Antibacterial Effects of Silver Nanoparticles Synthesized Using Verbena officinalis Leaf Extract on Yersinia ruckeri, Vibrio cholerae and Listeria monocytogenes (Antibacterial Effects of Silver Nanoparticle) Iranian Journal of Microbiology.

12- Research Title: Production of bioactive metabolites from different marine endophytic Streptomyces species against methicillin resistant Staphylococcus aureus (MRSA) and cancer cells. 3 Biotech.

13- Research Title: Assessment of Bacterial Deteriogens of Selected Lubricating Oils Used In Industrial Generators. Asian Journal of Biotechnology and Bioresource Technology 14- Research Title: Functional Evaluation and Physicochemical Characterization of a Lipopeptide Biosurfactant Produced by the Stenotrophomonas sp. IE-93. Iranian Journal of Science and Technology, Transactions A: Science

15- Research Title: Antagonistic activity of terrestrial actinomycetes against fish and shellfish pathogens. Revista de Bbiologia Marina Y Oceanografia 16- Research Title: Sero-prevalence of Toxoplasma gondii infection in healthy human population in Kashmir Valley, India. Journal of Transdisciplinary Biomedicine 17- Research Title: Influence of ZnO Nanoparticles on Candida albicans isolates biofilm formed on the urinary catheter Iranian Journal of Microbiology. 18- Research Title: Antibiotic sensitivity of microorganisms isolated from an oil polluted site in kokori, ethiope east, delta state, Nigeria. Biological Sciences and Pharmaceutical Research 19- Research Title: Anti-toxoplasma drug Discovery and Natural products: a brief overview. Frontiers in Clinical Drug Research - Anti-Infectives. 20- Research Title: In vitro synergistic activities of GSK656 with clarithromycin or azithromycin against Mycobacterium abscessus. Biomed Research International. 21- Research Title: Characterization of potential probiotic Bacillus sp. R2 and Planococcus sp. R11 isolated from gastrointestinal tract of sea bream (Sparus aurata). Egyptian Journal of Aquatic Biology and Fisheries.

22- Research Title: “Antibiotic sensitivity of microorganisms isolated from an oil polluted site in kokori, ethiope east, delta state, Nigeria” Biological Sciences and Pharmaceutical Research. KHOULOUD BARAKAT 9 Microbilogy Lab.

23- Research Title: “Anti-toxoplasma drug Discovery and Natural products: a brief overview”. Frontiers in Clinical Drug Research - Anti-Infectives 24- Research Title: In vitro synergistic activities of GSK656 with clarithromycin or azithromycin against Mycobacterium abscessus. Biomed Research International 25- Research Title: Characterization of potential probiotic Bacillus sp. R2 and Planococcus sp. R11 isolated from gastrointestinal tract of sea bream (Sparus aurata). Egyptian Journal of Aquatic Biology and Fisheries

26- Research Title: Construction of a novel lipid accumulating and lignin modifying oleaginous yeast consortium valued for biodiesel and bioremediation under cold condition. Journal of Hazardous Materials

27- Research Title:Dynamic Modelling of Tetrazolium Based Microbial Toxicity Assay – A Parametric Proxy of Traditional Dose-Response Relationship. Environmental Science and Pollution Research

28- Research Title: Effectiveness of Lactobacillus paracasei, Pediococcus acidilactis and Lactoccus lactis on Carbon Tetrachloride-Induced hepatotoxicity and nephrotoxicity of albino rats. Environmental Science and Pollution Research 29- Research Title: Antimicrobial materials and devices for biomedical applications. Frontiers in Clinical Drug Research-Anti-Infectives

30- Research Title: Anti-toxoplasma drug Discovery and Natural products: a brief overview. Frontiers in Clinical Drug Research-Anti-Infectives 31- Research Title: Antibacterial and Antibiofilm Activities of Malaysian Stingless Bee Trigona Honey. Frontiers in Clinical Drug Research-Anti-Infectives 32- Research Title: Distribution and molecular characterization of biosurfactant-producing bacteria. Biodiversitas Journal of Biological diversity 33- Research Title: Host-Directed, Antibiotic-Adjuvant Combination, and Antibiotic-Antibiotic Combination for Treating Multidrug-Resistant (MDR) Gram Negative Pathogens. Frontiers in Clinical Drug Research-Anti-Infectives

34- Research Title: Bis (2-ethylhexadecyl): a novel bioactive phthalate derivative from marine sediment-derived Streptomyces 10SAE against nosocomial strains. International Journal of Agriculture and Biology.

35- Research Title: Agro-Industrial Waste: New Source of Raw Material for the Control of American Foulbrood in Honey Bees. Frontiers in Clinical Drug Research-Anti-Infectives 36- Research Title: The ameliorative effects of Thymus vulgaris oil on hemato-biochemical and immune inflammatory parameters in rats infected experimentally with Escherichia coli O157:H7. Environmental Science and Pollution Research 37- Research Title: In Vitro Antibacterial Effect of Forsterite Nanopowder: Synthesis And Characterization Environmental Science and Pollution Research 38- Research Title: Antivirals to treat COVID 19. Frontiers in Clinical Drug Research-Anti- Infectives

39- Research Title: Therapeutic interventions against free radicals in viral diseases. The Journal Management System

Arbitration of Scientific Thesis

1. Thesis Title: Bioremediation of dyes using marine Bacteria. Botany and Microbiology Department, Faculty of Science, Alexandria University. 2. Thesis Title: Cadmium Bioremediation by an Extremophilic Bacterium from Wadi Al- Natrun. Botany and Microbiology Department, Faculty of Science, Alexandria University. 3. Thesis Title: “Studies on seaweeds as therapeutic agents against diarrhea causing organisms” Institute of Marine Science, University of Karachi, Karachi 75270, Pakistan 4. Thesis Title: Marine Fungal Exopolysaccharide: Production, Characterization and Valuable Applications. Botany Department, Faculty of Science, Tanta University. KHOULOUD BARAKAT 10 Microbilogy Lab.

5. Thesis Title: Production and Evaluation of the antitumor Glutaminase – Asparaginase by the filamentous fungi. Botany Department, Faculty of Science, Tanta University. 6. Thesis Title: Tailoring of Lipid Production from some Fungi Grown on Municipal Agriculture Wates “Botany and Microbiology Department, Faculty of Science, Alexandria University.

7. Thesis Title: “The role of the Endopolysaccharides from Mushroom and Green Tea Extracts against Carbon Tetrachloride-induced Hepatotoxicity in Rats” Botany Department, Faculty of Science, Tanta University.

8. Thesis Title: Protective effect of microbial melanin against some stress factors and its possible applications. Botany Department, Faculty of Science, Tanta University. 9. Thesis Title: The role of polysaccharide produced by Pleurotus sapidus as medical agent Botany Department, Faculty of Science, Tanta University. 10. Thesis Title: “Studies on seaweeds as therapeutic agents against diarrhea causing organisms” Institute of Marine Science, University of Karachi, Karachi 75270, Pakistan Conferences, Workshops and Invited Speaker:

I. Conferences

1. The Second International Conference Aquatic Resources "Integrated Coastal Zone management and Sustainable Development" ICAR-08. National institute of Oceanography and Fisheries, Alexandria, Egypt, 22- 25 November 2008. 2. The First Environmental Conference "Towards A Cleaner Mediterranean Region". Chambre De Commerce Egyptienne D'Alexandrie - Departemnte des Relations Internationales et des Expositions, Alexandria – Egypt, 13-16 June 2009.

3. The Second International Conference of Ecology "INCOECOLOGY 2009". ICOST in cooperation with Higher Institute of Public Health- Alexandria University- Egypt, 15-16 November 2009.

4. 1st Euro-Mediterranean Conference "Plant Natural Products from Biodiversity to Bioindustry" National Research Center, Cairo, Egypt, 8-10 December 2009. 5. The 3rd International Conference on Aquatic Resources "Prospects and Challenge" ICAR-. National institute of Oceanography and Fisheries, Alexandria- Egypt, 17- 20 November 2009.

6. The Sixth Conference of The Egyptian Society of Experimental Biology Faculty of Science, Minoufiya, Egypt, 27 February – 4 March 2010

7. Biovision Alexandria 2010 conference "New Life Science Future Prospects Biovision", Bibliotheca Alexandria- Egypt, 11- 15 April 2010

8. The 9th Annual International Conference of The Egyptian Society of Experimental Biology, Cairo University Conference Center, Egypt, 23 - 27 February 2013. 9. Science and Technology Education for Sustainable Development in the Arab Region Conference held in the Occasion of the TWAS-ARO 9th Annual Meeting, from 29 to 30 December 2013

10. The international Conferences of the Environmental Science (ICES), Cairo, Egypt, 20-21 May 2014

11. Drug Discovery and Therapy World Congress 2014" (DDTWC 2014) Boston, USA, from June 16th -19th, 2014

12. The first International ESSTB Conference "Transfer of Biomaterials into Technology, National Research Center, Egypt, March 1st -2nd, 2015. 13. Biovision Alexandria 2016 conference, Bibliotheca Alexandria- Egypt, 12-14 April 2016. 14. Bio Summit & Molecular Biology expo" Dubai, Arab Emirate, 10-11 October 2016. 15. The First International Conference and Exhibition on the Sustainable Development of Aquaculture, Cairo, Egypt, 20- 22 November 2017.

16. Arabian Aquaculture 2017 Conference, Alexandria, Egypt, 13-14 December, 2017. KHOULOUD BARAKAT 11 Microbilogy Lab.

17. The 17th Arab International Conference on Materials Science “Materials for Novel Applications”, Alexandria, Egypt, 18-20 December 2017. 18. The 2nd International Conference of Biotechnology and Environment, Alexandria, Egypt, 28- 30 March 2018.

19. Biovision Alexandria 2018 conference, Bibliotheca Alexandria- Egypt, 20-22 April 2018. II.

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