Data Entry Technician Assistant

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CURRICULUM VITAE

Michael R Muchow, Ph.D.

Cell: 301-***-****

********@*****.***

https://www.linkedin.com/in/michaelmuchow/

SUMMARY

Research and Applications Scientist with over 20 years of experience that includes laboratory research and customer-facing support, pre- and post-sales and training responsibilities in academic, government, biotech, CLIA clinical, and GMP pharmaceutical laboratory settings. Brings in-depth technical knowledge and expertise in molecular and cell biology, bioinformatics, genomics, immunology, genetics, cancer research, and statistics. Skilled in novel platform and assay development, and research project management experience.

TECHNICAL SKILLS/EXPERTISE

Computational: Common Next-Generation Sequencing tools—FASTQC, bwa, bowtie2, Magic-BLAST, GATK, samtools, bamtools, samblaster, bedtools, picard, sra_toolkit, sra_gatk, FreeBayes, HISAT2, TopHat2, cufflinks, MACS, IGV. Bash shell scripting, R (Bioconductor), Python (NumPy, Pandas, Matplotlib, Seaborn, SciPy), Perl, and SAS experience. High performance computing (HPC) on Linux cluster (SLURM). Oracle SQL. OpenStat and Excel. Eight graduate biostatistics credits with SAS lab. Frequentist and Bayesian statistics. Hidden Markov Models. SIMCA. Nucleic Acid: Next Generation Sequencing of DNA and analysis. Isolation and quantification

(spectrophotometry and fluorometry) of RNA and DNA. PCR for mRNA, miRNA, and methylated CpG islands. RT-PCR and Real-time PCR. Primer design. Flow cytometry with RNA probes. Oligonucleotide nylon and cDNA microarrays. Northern blotting. Ribonuclease protection assay. Transformations and transfections. Plasmid preparations. siRNA and shRNA. Whole-tissue, FFPE, and frozen tissue section in situ hybridization. Plasmid and Lentivirus cell-based reporter assays. Luminex bead-based DNA and RNA assays. Multiplex immunofluorescence. TUNEL.

Protein: Protein extraction and quantification (Bradford and BCA). Western blotting. Immunoprecipitation and co-immunoprecipitation. Immunocytochemistry. Whole-tissue immunohistochemistry. ELISA. Dual-labeling immunofluorescence including confocal detection. Protein expression vectors. Reverse hemolytic plaque assay. Chromatin immunoprecipitation. RIA and IRMA. Luminex bead-based immunoassays.

Cell Culture: Isolation and culture of primary cells (mouse, chicken, and PBMC). Culture of cell lines

(human, mouse, and rat). Culture of mouse and human tumor cells. Cell labeling, counting, and imaging. Media preparation.

Animal Handling: Breeding and genotyping of mice. Handling of mice, chickens, and turkeys. Dissection of mouse, turkey blastoderm, embryonic chicken, and post-hatch chicken. Cell line xenografts and gavage in nude mice. Tail snips and ear punches. Blood draws and injections. 2

RELATED EXPERIENCE

AstraZeneca, Frederick, MD

Senior Scientist, November 2021-present

AstraZeneca is a global, science-led biopharmaceutical business and its innovative medicines are used by millions of patients worldwide.

Manufacturing Sciences and Technology

Duties: Biomanufacturing Process Support. Process Engineering. Good Manufacturing Practice (GMP) & Good Documentation Practice (GDP). Technical writing of Change Controls, Impact Assessments, Validation Plans and Protocols, Final Technical Assessment Reports. Six Sigma. Raw Material Technical Evaluation. Basic laboratory work and ordering. Single Use Technology. Identifying Second Sources for material. Documentation of experiments in hard-copy and electronic Laboratory Notebook (eLN). Data analysis with SIMCA (Sartorius). Subject Matter Expert (SME) in various biomanufacturing areas. SWAT Team member—this group addresses critical supply chain issues. M-Star bioreactor modeling software. Created a dictionary list of company and industry acronyms with almost 900 entries that is searchable on the company website. Completed approximately 300 company Certificates and Learnings. STCube Pharmaceuticals, Gaithersburg, MD

Research Scientist, April 2021-June 2021

STCube is an immune-oncology company that is developing a first-in-class antibody targeting BTN1A1. BTN1A1 is an immune checkpoint protein that inhibits CD8 T cell proliferation and tumor growth in CD8+ T cells and has a high expression rate in solid tumors that do not respond well to existing PD-1/PD- L1 targeting immunotherapy.

Duties: Technical writing of journal article manuscripts describing the identification of BTN1A1 as an immune checkpoint by in vivo screening and BTN1A1 expression in normal tissues, tumor tissue microarrays, and immune cells, in vitro and in vivo validation of BTN1A1 as a novel immune checkpoint, increased tumor growth in mice transfected with mBTN1A1 expressing cells, anti-tumor activity of a humanized monoclonal antibody against hBTN1A1 in a human immune environment in vitro and ex vivo, the slower growth of BTN1A1 knockout (KO) tumors compared to wild-type in mice, tumor-infiltrating lymphocyte expansion and anti-tumor activity in BTN1A1 KO tumors, restoration of tumor cell growth by CD8+ T cell depletion, development of autoimmune phenotypes in BTN1A1 KO mice, efficacy of anti-mBTN1A1 surrogate antibody in mice. Editing of Study Reports. Novel Microdevices, Baltimore, MD

Staff Scientist, Nucleic Acid, December 2020-January 2021 Novel Dx detects the genetic material of pathogens using Nucleic Acid Amplification techniques. These are the same methods that are utilized in large reference laboratories Novel Dx delivers lab-quality results in under 30 minutes so patients can be tested and treated in the same visit. 3

Duties: Bioinformatics. PCR primer design. PCR. Loop-mediated Amplification (LAMP). Johns Hopkins University School of Medicine, Baltimore, MD Stanley Division of Developmental Neurovirology

Senior Research Specialist, February 2020-September 2020 Laboratory research focused on how to determine how Toxoplasma gondii infection is involved in the etiology of psychosis, especially schizophrenia and bipolar disorder. T. gondii is a protozoan parasite that infects approximately ~ 25% of the world's human population. As a neurotropic parasite, Toxoplasma causes significant brain and behavioral anomalies in humans and other mammals. Infection may cause changes in host gene expression, microRNA, neurotransmitters, signaling pathways, and behavioral abnormalities.

Duties: Western blotting. Fluorescent Immunohistochemistry on FFPE tissue sections. Planning the lab’s experiments. Data analysis.

NIH/NLM/NCBI

Volunteer Software Tester, April-May 2019

Software alpha-tester for Blast+ Docker container. Volunteered at NCBI to test their new Docker container implementation of Blast+, the command line version of BLAST. Downloaded and installed Docker software. Downloaded existing BLAST databases and created custom databases (protein and nucleic acid). Searched databases for protein and nucleic acid sequences using varying search parameters. Tested Docker container on Google Cloud Platform. Recorded commands, output, and error messages. Seneca Solutions, Remote

Geneticist, August-September 2018 (Temporary Federal contract) Department of Defense contracted consultant as a Genetics Subject Matter Expert for the United States Air Force AFMSA (Air Force Medical Support Agency)/SG5R personalized medicine clinical and research efforts.

Duties: Evaluation of detecting G6PD deficiency, ABO blood group, and Sickle cell trait using genetic testing. Assessing polygenic risk scores for acquired/non-syndromic/noise-induced hearing loss. Determining the best SNP targets to predict preventable hearing loss disability. Production of monthly progress reports.

Children’s National Medical Center, Washington, DC Molecular Genetics Staff Scientist II, September-December 2017 The Molecular Genetics Group in the Laboratory of Medicine performs physician-ordered genetics-based diagnostic assays in a CLIA laboratory.

Duties: Used Illumina’s BaseSpace to generate bam files using Isaac and BWA Apps with variant calling, 4

and then merged vcf files using VCAT App; QC inspection of all outputs. Analyzed patient data generated by SNP-specific TaqMan assays and Illumina’s NGS TruSight One Sequencing Panels. Used Sanger sequencing to examine ambiguous NGS sequences (selecting appropriate commercial primers, if available; designing own, if not). Wrote Validation Plan for in-house Fragile X testing using ABI’s Genetic Analyzer. Designed PCR primers to specifically amplify NCF1 and not its highly identical pseudogenes NCF1B and NCF1C for Sanger sequencing detection of genetic disease variant. Software: Illumina BaseSpace, QuantStudio (ABI) for TaqMan SNPs, Integrated Genome Viewer (IGV; Broad) for NGS bam files, Mutation Surveyor (SoftGenetics) for Sanger, NextGENe and GeneMarker

(SoftGenetics), bash shell scripting. Supervision of Medical Technicians. National Institute of Standards and Technology, Gaithersburg, MD Research Biologist (Bioinformatics), June 2015-September 2017 Research project is a collaboration with MedImmune (AstraZeneca) to study the genomic stability of Chinese hamster ovary (CHO) cell lines during large-scale production of biopharmaceuticals. The goals of the project are to measure the changes in the genomes of CHO cells that occur during prolonged passaging in culture which may lead to changes in the expression of recombinant proteins. Methods— including microarrays, Next-Generation Sequencing (NGS), quantitative PCR, and droplet digital PCR— will be developed to detect genomic changes to accurately monitor changes during production of therapeutic proteins.

Duties: Isolated and analyzed CHO gDNA using Affymetrix expression microarrays. Analysis of transfected CHO cell Next-Generation Sequencing data generated by Illumina HiSeq X 10: Bio-Linux, FASTQC, bwa, bowtie2, GATK, samtools, bamtools, bedtools, FreeBayes, IGV. Mentoring of Student Volunteers.

Research Collaborator with NCBI (March 1st-April 14th, 2017) developing bioinformatic pipelines for identifying transgene insertion sites and plasmids in E. coli strains. Biomedical Research Institute of New Mexico, Albuquerque, NM Research Associate, March-August 2014

Primary focus of the laboratory is lung cancer research using cell culture, mouse models, and human clinical trials.

Duties: Served as Lab Manager. Performed cell culture, RNA isolation, and Luminex bead-based gene expression assays. Performed gavage, xenograft injection, blood draw, and dissection of mice. Training in local IRB Standards/HIPAA protocols and database management for pre-clinical and Phase I Clinical Trials.

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Affymetrix (Panomics products), Santa Clara, CA

Field Applications Scientist II, June 2011-December 2012 Company provides products for biological research that permits multiplexing detection and analysis of DNA, RNA, and cytokine targets using Luminex bead-based branched DNA (bDNA) or sandwich antibody assays; DNA, RNA, and microRNA single probe assays; and in situ hybridization detection of targets in cells and tissue sections.

Duties: Trained and supported customers on the use of Panomics QuantiGene bDNA Luminex bead- based assays for RNA, DNA, and Procarta cytokine detection and quantification, and bDNA-based ISH assays with demonstrations and on-site training. Provided post-assay data analysis and assay summary reports to customers. Conducted pre- and post-sales consultations. Provided experimental design advice. Provided post-assay troubleshooting. Provided assay feedback to R&D and Sales and Marketing Departments. Beta-tested assays in R&D development. Performed assays on customer samples in-house. Maintained customer information in SalesForce CRM. Provided technical support to users and answered customer inquiries. Maintained records of customer communications and technical support functions through available tools. Worked with sales manager and representatives to execute sales strategies in different territories. Assisted in design and delivery of formal training courses and participation in product pre-launch activities.

SABiosciences (Qiagen), Frederick, MD

Application Scientist, August 2008-November 2010

Company provides products for biological research that permits high-performance analysis of DNA, RNA, epigenetic, and microRNA targets in biological pathways associated with specific diseases. Products include oligo microarrays; real-time PCR for mRNA, miRNA, and methylated CpG islands; chromatin immunoprecipitation assays; plasmid and Lentivirus cell-based reporter assays; protein expression vectors; siRNA and shRNA plasmids; and ELISA and immunocytochemistry. Duties: Responsible for providing technical support to existing customers using SABiosciences products by possessing an in-depth understanding of all products. Products include oligo microarrays; real-time PCR for mRNA, miRNA, and methylated CpG islands; chromatin immunoprecipitation assays; plasmid and Lentivirus cell-based reporter assays; protein expression vectors; siRNA and shRNA plasmids; and ELISA and immunocytochemistry. Explaining principles, protocols, and data analysis to customers by oral and written communication. Properly diagnosing the needs of new customers to provide recommendations and quotations for new products and services. Responsibilities involve 50% post-sales support and 50% pre-sales qualification and follow-up. Customers are located all over the world and contact SABiosciences regularly for assistance on their research, particularly in the areas of gene expression and characterization.

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National Institutes of Health, Bethesda, MD

National Institute of Child Health and Human Development Postdoctoral Fellowship, July 2006- July 2008

Primary focus of the laboratory is research into the inherited endocrine tumors syndrome Carney Complex and sporadic tumors of the pituitary and the adrenal glands. Duties: Primary Investigator: “Characterization of mouse models for tumorigenesis observed in patients with Carney Complex”; NIH Animal Study Protocol 06-033. Managing the laboratory’s mouse colony. Writing Animal Study Protocol. Breeding and genotyping of mice. Dissection of mice. Isolation and culture of mouse primary cells. Culture of human and mouse cell lines. Protein extraction and quantification. Western blotting. Immunoprecipitation and co-immunoprecipitation. Isolation and quantification of RNA and DNA. Nylon oligonucleotide microarrays. PCR, RT-PCR, and Real-time PCR. Plasmid preparations. Transformations and transfections. Designing of experiments. Collection and statistical analysis of data. Writing and editing of papers and abstracts. University of Maryland, College Park, MD

Department of Animal and Avian Sciences

Laboratory Research Technician, July 1998-June 2006 Primary focus of the lab research is molecular and cellular endocrinology of animal growth and development.

Duties: Managing the day-to-day operation of the lab. Training new personnel. Maintaining lab equipment. Purchasing and maintaining inventory. Accounting of invoices. Disposal of hazardous and radioactive waste. Writing and editing of papers, abstracts, and SOPs. Designing of experiments. Collection and statistical analysis of data. Isolation and culture of primary cells. Culture of cell lines. Dissection of embryonic tissues. Reverse hemolytic plaque assay. Immunocytochemistry. Protein extraction and quantification. Western blotting. Immunoprecipitation. Isolation and quantification of RNA and DNA. Northern blotting. Ribonuclease protection assay. Plasmid preparations. Transformations and transfections. siRNA. RIA and IRMA. Whole-tissue in situ hybridization. Whole-tissue immunohistochemistry. PCR, RT-PCR, and Real-time PCR. Primer design. Dual-labeling immunofluorescence. Paraffin embedding of embryos and tissues. United States Department of Agriculture, Agriculture Research Service, Beltsville, MD Gamete and Germplasm Physiology Laboratory

Laboratory Technician Assistant, July 1996- May 1998 Primary focus of the lab research is cellular physiology of turkey reproduction. Undergraduate training position (part-time).

Duties: Isolation, fixation and embedment, and sectioning of blastoderms with diamond knife microtome. Preparation of lab buffers. Cell labeling and counting. Light microscopy. Data entry and statistical analysis. Assisting the lab technician. Dissection. General lab maintenance. Apoptotic TUNEL assay. 7

EDUCATION

University of Maryland, College Park, MD

Ph.D. in Molecular and Cell Biology

Dissertation: “Identification of a single nucleotide polymorphism associated with adiposity following transcriptional profiling of gene expression in the anterior pituitary gland”. Advisor: Tom E Porter

University of Maryland, College Park, MD

M.S. in Animal Science

Thesis: “Ontogeny and regulation of thyroid-stimulating hormone beta during chicken embryonic development”.

Advisor: Tom E Porter

University of Maryland, College Park, MD

B.S. in Biological Sciences

Area of Concentration: Marine Biology

University of Maryland, University College, MD

Graduate Certificate in Bioinformatics

Courses:

Introduction to Bioinformatics

Advanced Bioinformatics (Perl programming)

Statistical Processes for Biotechnology (Hidden Markov Models and Bayesian statistics) Relational Database Systems (SQL)

Data Structures and Algorithms

WORKSHOPS AND HACKATHONS

FAES Workshop "Bioinformatics Analysis of Next-Generation Sequencing Data (BioTech 45)" 2016 Topics: Overview on Bioinformatics Tools: Galaxy (Pre-processing, Format Conversion, etc.); Databases and Tools; DNA-Seq; SNP Callers; RNA-Seq; CHIP-Seq; Epigenomics; Data Visualization; Comparative Genomics and Genome Alignment (biology + software); Tutorial & Laboratory for de novo Assembly. NCBI's Next-Generation Sequencing Online Workshop (NOW) 2015. Description: Covering the basics of NGS data, preprocessing, quality control and alignment strategies for both DNA-Seq and RNA-Seq, as well as a brief discussion of downstream analysis. Additionally, we will demonstrate how to leverage BLAST tools for NGS analysis. Next, participants will apply a selection of RNA-Seq alignment algorithms over three days (1-2 hours per day), mapping RNA-Seq data to GRCh38 chromosome 20. Participants will compare the results of these mappers for specific genes. 8

Attended NCBI Virus Hunting in the Cloud Codeathon v2, November 4-6, 2019—developed FIVE

(Federated Index of Viral Experiments) API database Attended NCBI Computational Medicine Hackathon. June 10-11, 2019—developed a HaploRNA-Seq pipeline for Population Defined RNA-Seq Dosage

Attended NCBI Bioinformatics Hackathon, March 19-21, 2018—developed STREAMclean, a simple command-line tool to map and filter SRA reads with high accuracy Attended NCBI Bioinformatics Hackathon, August 14-16, 2017—developed educational Jupyter Notebooks for bioinformatics tutorials

Attended NCBI Genomics Hackathon, March 20-22, 2017—developed Squidstream, a tool for genomic feature ID conversion

Attended NCBI, NLM, NHGRI Hackathon, January 9-11, 2017—developed PubRunner, a text-mining tool (PubRunner.org)

AWARDS AND PATENTS

* 20th Annual Invention of the Year Awards, Office of Technology Commercialization, UMCP. Runner- up: Life Sciences. “Genetic polymorphisms associated with body fat”

* US Patent application 11822540: “Genetic polymorphisms associated with body fat”

* 1st place presentation of excellence in poultry research. 16th Annual Symposium, Department of Animal and Avian Sciences, UMCP.

* 2nd place presentation of excellence in poultry research. 14th Annual Symposium, Department of Animal and Avian Sciences, UMCP. ARTICLES IN REFEREED JOURNALS

1. Martí-Carreras J, Gener AR, Miller SD, Brito AF, Camacho CE, Connor R, Deboutte W, Glickman C, Kristensen DM, Meyer WK, Modha S, Norris AL, Saha S, Belford AK, Biederstedt E, Brister JR, Buchmann JP, Cooley NP, Edwards RA, Javkar K, Muchow M, Muralidharan HS, Pepe-Ranney C, Shah N, Shakya M, Tisza MJ, Tully BJ, Vanmechelen B, Virta VC, Weissman JL, Zalunin V, Efremov A, Busby B. NCBI’s Virus Discovery Codeathon: Building the “FIVE”—Federated Index of Viral Experiments API database. (2020) Viruses Dec 10;12(12):1424. doi: 10.3390/v12121424. PMID: 33322070; PMCID: PMC7764237.

2. Anekalla KR, Courneya JP, Fiorini N, Lever J, Muchow M, Busby B. PubRunner: A light-weight framework for updating text mining results. (2017) F1000 Research, 6:612 (doi: 10.12688/f1000research.11389.1)

3. Almeida MQ, Muchow M, Boikos S, Bauer AJ, Griffin KJ, Tsang KM, Cheadle C, Watkins T, Wen F, Starost MF, Bossis I, Nesterova M, Stratakis CA (2010) Mouse Prkar1a haploinsufficiency leads to an increase in tumors in the Trp53+/- or Rb1+/- backgrounds and chemically induced skin papillomas by dysregulation of the cell cycle and Wnt signaling. Hum Mol Genet 19:1387-98. 4. Meoli E, Bossis I, Cazabat L, Mavrakis M, Horvath A, Shiferaw ML, Fumey G, Perlemoine K, Muchow M, Robinson-White A, Weinberg F, Nesterova M, Patronas Y, Groussin L, Bertherat J, Stratakis CA (2008) Protein kinase A (PKA) effects of an expressed PRKAR1A mutation associated with aggressive tumors. Cancer Res 68:3133-41.

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5. Pasini B, McWhinney SR, Bei T, Matyakhina L, Stergiopoulos S, Muchow M, Boikos SA, Ferrando B, Pacak K, Assie G, Baudin E, Chompret A, Ellison JW, Briere JJ, Rustin P, Gimenez-Roqueplo AP, Eng C, Carney JA, Stratakis CA. (2008) Clinical and molecular genetics of patients with the Carney-Stratakis syndrome and germline mutations of the genes coding for the succinate dehydrogenase subunits SDHB, SDHC, and SDHD. Eur J Hum Genet 16:79-88.

6. Pasini B, Matyakhina L, Bei T, Muchow M, Boikos S, Ferrando B, Carney JA, Stratakis CA. (2007) Multiple gastrointestinal stromal tumors caused by platelet-derived growth factor receptor α gene mutations: a case associated with a germline V561D defect. J Clin Endocrinol Metab 92:3728-32. 7. Matyakhina L, Bei TA, McWhinney SR, Pasini B, Cameron S, Gunawan B, Stergiopoulos SG, Boikos S, Muchow M, Dutra A, Pak E, Campo E, Cid MC, Gomez F, Gaillard RC, Assie G, Fuzesi L, Baysal BE, Eng C, Carney JA, Stratakis CA. (2007) Genetics of Carney triad: recurrent losses at chromosome 1 but lack of germline mutations in genes associated with paragangliomas and gastrointestinal stromal tumors. J Clin Endocrinol Metab 92:2938-43.

8. Jenkins SA, Muchow M, Richards MP, McMurtry JP, Porter TE. (2007) Administration of adrenocorticotropic hormone during chicken embryonic development prematurely induces pituitary growth hormone cells. Endocrinology 148:3914-21.

9. Porter TE, Ghavam S, Muchow M, Bossis I, Ellestad L. (2006) Cloning of partial cDNAs for the chicken glucocorticoid and mineralocorticoid receptors and characterization of mRNA levels in the anterior pituitary gland during chick embryonic development. Domest Anim Endocrinol 33:226-39. 10. Ellestad LE, Carre W, Muchow M, Jenkins SA, Wang X, Cogburn LA, and Porter TE (2006) Gene expression profiling during cellular differentiation in the embryonic pituitary gland using cDNA microarrays. Physiol Genomics 25:414-25.

11. Muchow M, Bossis I, Porter TE (2005) Ontogeny of pituitary thyrotrophs and regulation by endogenous thyroid hormone feedback in the chick embryo. J Endocrinol 184:407-16. 12. Bossis I, Nishimura S, Muchow M, Porter TE (2004) Pituitary expression of type I and type II glucocorticoid receptors during chicken embryonic development and their involvement in growth hormone cell differentiation. Endocrinology 145:3523-3531. ABSTRACTS AND OTHER PROFESSIONAL PAPERS PRESENTED

1. Timothy Moeller, Caitlin Brown, Mike Muchow, Brendan Yee (2012) Transcript Regulation of 18 ADME Genes by Prototypical Inducers in Human Hepatocytes. Program 18th North American Regional ISSX Meeting.

2. Almeida MQ, Muchow M, Boikos S, Bauer AJ, Griffin KJ, Tsang KM, Cheadle C, Watkins T, Wen F, Starost MF, Bossis I, Nesterova M, Stratakis CA (2010) Mouse Prkar1a haploinsufficiency leads to an increase in tumors in the Trp53+/- or Rb1+/- backgrounds and chemically-induced skin papillomas by dysregulation of the cell cycle and Wnt signaling. Program 92th Annual Meeting of the Endocrine Society.

3. Liu X, Cogburn LA, Muchow M, Le Bihan-Duval E, Simon J, Porter TE (2009) Identification of Novel Polymorphisms Associated with Levels of Body Fat in a Unique Genetic Model for Obesity. Program 91th Annual Meeting of the Endocrine Society.

4. Almeida MQ, Muchow M, Cheadle C, Watkins T, Wen F, Nesterova M, Stratakis CA (2009) Prkar1a Haploinsufficiency in Mice Increases Susceptibility to Skin Carcinogenesis. Program 91th Annual Meeting of the Endocrine Society.

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5. *Muchow M, Nesterova N, Abu-Asab M, Tsokos M, Cheadle C, Watkins T, Stratakis C (2008) Induction of skin tumors in the prkar1a+/- mouse model for Carney Complex. Program 90th Annual Meeting of the Endocrine Society.

*Invited poster, ENDO 08 Trainee Poster Competition. 6. Muchow M, Bimpaki E, Stratakis C (2008) Gene expression in adrenal tumors of patients with Carney Complex. Program 90th Annual Meeting of the Endocrine Society. 7. TE Porter, LE Ellestad, MS Byerly, M Muchow, SA Jenkins, SE Higgins, M Mukherjee, J Narayana, J Saliba, and K Heuck (2008) Analysis of global gene expression in the avian neuroendocrine system. 9th International Symposium on Avian Endocrinology.

8. Porter TE, Ellestad LE, Muchow M, Byerly MS, Simon J, Cogburn LA (2007) Analysis of global gene expression in the neuroendocrine system of chickens genetically selected for high and low body weight and high and low body fat. Program 15th Plant and Animal Genome Conference. 9. Bauer J, Muchow M, Griffin KJ, Stein E, Hoffmann V, Stratakis CA (2007) Osteosarcomas, Lymphangiosarcomas and Cardiomegaly Develop in prkar1a+/- Mice Bred into the p53+/- Background. Program 89th Annual Meeting of the Endocrine Society. 10. Zager E, Moore J, Muchow M, Porter T, Castonguay TW (2005) Interference RNA Knockdown of GH3 and GH4C1 Pituitary Cell 11 Beta Hydroxysteroid Dehydrogenase-1. Program 35th Annual Meeting of the Society for Neuroscience.

11. Muchow M, Simon J, Cogburn LA, Porter TE (2005) Identification of Differentially Expressed Genes in the Anterior Pituitary Gland of Fat and Lean Animals. Program 87th Annual Meeting of the Endocrine Society.

12. Porter TE, Ellestad L, Carre W, Wang X, Muchow M, Cogburn LA (2004) Global gene expression profiling of the chicken neuroendocrine system using tissue-specific cDNA microarrays. Program 12th Plant and Animal Genome Conference.

13. Porter TE, Ellestad L, Carre W, Jenkins SA, Wang X, Muchow M, Cunningham C, Cogburn LA

(2003) Analysis of Global Gene Expression with a Chick Neuroendocrine System cDNA Microarray. Program 85th Annual Meeting of the Endocrine Society. 14. Muchow M, Porter TE (2002) Regulation of thyroid stimulating hormone (TSH) production in vivo during chicken embryonic development. Program 84th Annual Meeting of the Endocrine Society. 15. Ghavam S, Muchow M, Porter TE (2002) Cloning of a partial cDNA for the chicken mineralocorticoid receptor. Program 84th Annual Meeting of the Endocrine Society. 16. Muchow M, Porter TE (2000) Location and ontogeny of thyrotrophs during chicken embryonic development. Program 92nd Annual Meeting of the American Society of Animal Science. 17. Porter TE, Muchow M, Bossis I (2000) Use of an antiserum to rat thyroid stimulating hormone beta

(TSHβ) for studies of TSHβ production in chickens. Program 7th International Symposium on Avian Endocrinology.

18. Porter TE, Muchow M (1999) Retinoic acid augments corticosterone-induced somatotroph differentiation. Program 81st Annual Meeting of the Endocrine Society. TEACHING EXPERIENCE

University of Maryland, College Park, MD

Teaching Assistant ANSC 214 Applied Animal Physiology Laboratory 11

REVIEWER

Endocrine-Related Cancer

Contact this candidate