Xiaohua Tian, M.S., CHT(ABHI)

(U.S. citizen)

Address: *** ******** **** ******, *******, GA 30033

Telephone: 404-***-****

E-mail: adsjbn@r.postjobfree.com

EDUCATION BACKGROUND

AUG/1990-JUL/1993 Master’s Degree (Public Health)

Tianjin Medical University, Tianjin China

AUG/1982-JUL/1987 Bachelor’s Degree (Medicine) (equal to M.D. in USA)

Tianjin Medical University, Tianjin China

MOLECULAR BIOLOGICAL TECHNIQUES.

Next generation sequencing using Miseq, including library prep and sequencing; also perform RNA library prep for Hiseq.

DNA and RNA isolation from tissue and cultured cells.

PCR and Real time PCR

Western blotting; Immunoprecipitation;

ELISA detecting cytokines from cell culture supernatants;

Tissue sectioning and embedding(frozen and paraffin); H&E staining and immunoflourescent staining for tissue sections and cultured cells;

Regular microscope and fluorescent microscope;

Isolation of primary epithelial cells and endothelial cells from animal organs(lungs);

Cell culture, including primary epithelial cells and endothelial cells and cell lines.

Cell transfection by electroporation, Fugen 6 and Lipofectamine, and viruses;

ANIMAL EXPERIMENT TECHNIQUES

Skillfully handle animals and perform surgery and microsurgery on rats or mice

Creating animal model of focal or global cerebral ischemia/reperfusion;

Creating head trauma model of rats;

Femoral vein and artery catheterization;

Regular physiological tests on animals like cerebral blood flow;

Behavior tests like learning behavior and motor behavior;

TECHNICAL SKILL RELATED TO HLA TYPING

Next generation sequencing using Miseq, including library prep and sequencing; also perform RNA library prep for Hiseq.

Isolation of lymphocytes from peripheral blood, lymph nodes and spleen.

Extraction of DNA from peripheral blood.

Flow cytometry crossmatch and PRA and Immunophenotyping using BD FACS Calibur, and FC500 Beckman Coulter .

DNA typing of HLA Class I and class II by PCR-SSP, using Invitrogen Tray and Olerup Tray.

DNA typing by reverse sequence specific oligonucleotides (rSSO), using Luminex (LabScan 100)

Antibody screening (PRA) by Luminex (LabScan 100).

Daily quality control.

Data entry with software “HISTOTRAC” and Cerner, and paperwork handling for patients and samples.

Coordinate dialysis centers for sample delivery and pick-up.

Good computer skills: Microsoft Word, Excel, Photoshop, and Power point.

Excellent interpersonal skill.

WORKING EXPERIENCE

August/2020-present Senior Scientist

Caprico Biotech INC, antibody production

April/2019-May/2020 HLA technologist(Medical Technologist)

MD Anderson Cancer Center Histocompatibility Lab, Performing HLA typing

Jun/2016-present Scientist

Omega Biotek Company Atlanta GA, performing NGS using Miseq. Performing DNA and RNA library prep and sequencing.

Jun/2014-May/2016 HLA Technologist(Medical Technologist)

Georgia Regents University Healthcare HLA lab at Augusta, GA. Performing DAN typing, flow crossmatch and PRA, and antibody screening, using flow cytometry Coulter FC 500 and Luminex( Lab scan 100), and serological methods.

Jun/2012-Jun/2014 HLA Technologist(Medical Technologist)

Working at the University of Chicago, University Hospital Transplant and Immunogenetics Lab, performing Flow Crossmatch and PRA, and antibody screening (ID and SA) using Beckman Coulter FC 500, and Luminex (Labscan 100).

Oct/2009-June 2012 Research Associate

Morehouse School of Medicine, Cardiovascular Research Institute

Research on (1) Epigetics: methylation of non-coding RNA(Alu RNA),

and DNA related to cancer, cardiovascular disease and other diseases; (2) Identification of alternative splicing isoforms from NCF-1 and its pseudogene and their function. Designing and performing experiments, supervising Ph.D students, and drafting manuscripts for research papers and proposals.

Oct/2008-Oct/2009 Postdoctoral associate and Lab Manager

Emory University, School of Medicine, Dept. of Pediatrics, Section of GI. Research on functional consequences and mechanisms of IL-27 on inflammatory bowel disease, and regulatory effect of miRNA on IL-27 in macrophages; building up the lab, and drafting projects, ordering staffs, performing experiments and writing paper, supervising technicians.

Nov/2006-Sep/2008 Postdoctoral Fellow

Tulane University Health Science Center Department of Medicine Section of Pulmonary Diseases, New Orleans, LA.

Research on“miRNA modulates epithelial-mesenchymal transition in lung fibrosis and cancer”, and “Regulative mechanisms of G protein-coupled receptors in chlorine-induced acute lung injury” using molecular biological techniques and animal experiments.

May/1998-Oct/2006 Postdoctoral Researcher/Instructor

Louisiana State University Medical Center Neuroscience Center

Research on molecular biological mechanisms of bioactive lipids in brain ischemia, and preclinical study on compounds (PAF-receptor antagonists) with mouse model of cerebral ischemia /reperfusion.

Aug/1993-Jul/1994 Physician/Assistant Professor

Tianjin Medical University, Tianjin China.

Teaching Biochemistry and Nutriology.

Research on “the effects of trace elements on blood glucose, insulin response, glucose tolerance and lipoprotein metabolism in noninsulin dependent diabetics and hyperlipidemic patients”; “Effect of dietary habit and exercise on lipid metabolism in obese children”.

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