Shivraj Tyagi

Brookline, ma

adscsv@r.postjobfree.com

617-***-**** (cell)

SUMMARY

Highly motivated biomedical Scientist

Reliable team leader with vast experience in cell biology/molecular biology/biochemistry/Stem cell biology. I have extensive experience in RNA and DNA molecules-particularly isolation and purification, nanodrop-quantification and handling/manipulation and cDNA and RT-PCR/Q-PCR commonly used in most biomedical/clinical research projects.

Strong work ethic and demonstrated track record of successfully research and development

Excellent SAM (Science-Administrative-Management), organizational skills to execute more then one research projects at a given time. Working knowledge of microsoft office applications (such as excel, powerpoint, word and outlook)

EXPERIENCE HIGHLIGHTS

Akebia Therapeutics, Inc October 2021 - present

· Performed in vivo experiments at vivarium using rats/neonates and mice

· Screened new compounds for chronic kidney diseases and analyzed various pharmacological aspects using PK/PD based assays

· Helped in various wet-lab experiments (Protein, DNA and RNA analysis) for new clinical research projects

Boston Children’s Hospital/Harvard Medical School March 2021 - May 2021

Clinical Research Manager-II

· Limited experience in coordinating and executing several multi-center clinical projects (“FORCE” and Project SINGULAR)

· Assisted in data analysis/QA using AMBRA and RedCap programs

· Supervised a team of research associates, IT-professional, ML and AI-staff

Weill Cornell Medicine, Manhattan, NY campus Jan 2019 – February, 2021

Assistant Professor of pharmacology research in Medicine:

First hand experiemce in completing several basic and clinical research projects at WCM-NYP hospitals. Mainly analyzing RNA, DNA and protein assays, mitophagy/apoptotic mtDNA biomarkers and cytokines profiles from various fresh control and patient samples (such as plasma and urine) as well as samples from our WCM-biobank inventories

Worked as a team member in multi Centre clinical trials management (Mass general hospital, Brigham And Women's Hospital & Duke & Weill Cornell) multi-centres research projects.

Provides clinical biomarker operations expertise to ensure operational feasibility and delivery

Managed inventories of bio bank clinical samples (plasma & urine samples)

Supervised post doctor fellows/ Graduate students and technician in IHC – immunohistochemistry projects at our division PCCM. I used GFP, RFP, DAPI H/E for IF – Immunofluorescence/ IHC – Immunohistochemistry

Involved in various Lung and Kidney fibrosis research projects (such as CKD, Sepsis and COPD/pulmonary diseases (Lead role in both Fibrosis and COPD human and mice models)

Lead role and responsible for executing all SAM (Science-Administration-Management) related various tasks/responsibilities (including IRB and IACUC protocols) at WCM-Dean Prof. Augustine Choi research labs

Boston BioProducts, Inc, Ashland, MA April, 2015 – August, 2018

Director of Research & Development:

Supervised and direct R&D related contracts/sub-contract research projects involved molecular biology (RNA, cDNA synthesis, Q-PCR (RT-PCR), Cell biology (IF and various substrate coating of 384 wells used for tissue engineering

Direct as well as involved in preparing SOPs, Certificate of Analysis and all kinds of labels preparation

Lead role in developing new kits and next generation molecular biology reagents: for RNA and DNA isolation, protection and analysis, developing biomarkers, magnetic beads and commonly used enzymes for cell biology (endotoxin detection and mycoplasma detection)

Lead role in performing quality control analysis of all custom reagents and testing of Endotoxin, RNAase and DNAase levels in all laboratory bio-reagents/solutions

Brigham and Women’s Hospital, Harvard Medical School, Boston, MA February, 2005 – December, 2013

Instructor of Medicine

Authored and served as PI on 2 grants (Project Title: The Role of Bronchio-Alveolar Stem Cells in Cigarette Smoke-Related Emphysema)

$100K: American Thoracic Society/ Alpha-1 Foundation award: January 2008 to Dec. 2010 (Role PI)

$325K: FAMRI: Clinical Innovator Award # 072161-CIA, July 2008 to June 2012 (Role PI)

Involved in the isolation, characterization, and quantification of lung progenitor cells to study lung homeostasis/repair during normal and pathophysiological conditions in mouse, sheep, and human models

Helped develop the technology that proved that autologous cell-based therapy in higher animal models can be correlated to human models

Led human biomarker emphysema/COPD-clinical research projects that help in early detection of chronic lung diseases caused by direct or indirect cigarette smoke exposure

Served as Immunohistochemistry Core co-director in the Pulmonary and Critical Care Division

Involved in an asthma-related project – “Pharmacogenetic Linkage of Corticosteroid Response: Asthma Therapy Implications”

Collaborated with Dana Farber Cancer Institute on a cell-based therapy program in a translational/clinical setting for the treatment of human emphysema/COPD patients. Helped them in a PACT-program

Supervised graduate and research fellows

Education

Ph.D. in Medical Biochemistry (Pulmonary and hepatic rodent-models were used for assessing drug metabolizing enzymes) from University of Delhi, India

MS in Medical school) Medical Biochemistry from University of Delhi, India

MS in Organic Chemistry from Meerut University, India

BS in Biology and Chemistry from University of Delhi, India

SELECTED PUBLICATIONS / RESEARCH SUPPORT

Selected few publications (from more than 35) in adult progenitor/stem cells, bioinformatics-biomarkers, lung development and xenotransplantation: (peer reviewed research articles in the world class scientific journals, Such as Nature Medicine, JBC, PNAS, Biochemistry etc., not included herein).

1. Qiu, W., Rogers, A.J., Damask, A., Raby, B.A., Klanderman, B.J., Duan, Q.L., Tyagi, S.R., Niu, S., Anderson, C., Cahir-McFarland, E., Mariani, T.J., Carey, V., Tantisira, K.G., Pharmacogenomics: Novel Loci Identification via integrating gene differential analysis and eQTL Analysis, 2014, Hum Mol Genet. 2014 Sep 15;23(18):5017-24

2. Ingenito, E.P., Tsai, L., Murthy, S., Tyagi, S.R., Mazan, M., and Hoffman, A., Autologous Lung-Derived Mesenchymal Stem Cell Transplantation in Experimental Emphysema. Cell Transplantation. 2012, 21, 175-189.

3. Bhattacharya, S, Tyagi, S.R., Srisuma, S, DeMeo, D.L., Shapiro, S.D., Bueno, R, Silverman, E.K., Reilly, J.J., and Mariani, T.J., Peripheral Blood Gene Expression Profiles in COPD Subjects, J Clin Bioinformatics. 2011, 1, 12.

4. Hoffman, A.M., Paxson, J.A., Mazan, M.R., Davis, A.M., Tyagi, S.R., Murthy, S., and Ingenito, E.P., Lung Derived Mesenchymal Stromal Cell Post-Transplantation Survival, Persistence, Paracrine Expression, and Repair of Elastase- Injured Lung. Stem Cells, and Development, 2011, 20, 1779-1792.

5. Sorachai, S, Bhattacharya, S, Simon, D.M., Solleti, S. K., Tyagi, S.R., Barry Starcher, B. and Mariani, T.J., FGF Signaling controls epithelial-mesenchymal interactions necessary for alveolar elastogenesis. Am. J. Res. Cri Care Med. 2010, 181, 838-850.

6. Yamashita, K, Öllinger, R., McDaid, J., Sakahama, H., Wang, H., Tyagi, S.R, Csizmadia, E., Smith, N.R., Soares, M.P., and Bach, F.H. Heme Oxygenase-1 is Essential for and Promotes Tolerance to Transplanted Organs. The FASEB Journal 2006; 20: 776-778.

7. Öllinger R, Bilban M, Erat A, Froio A, McDaid J, Tyagi S.R., Csizmadia E, Graça-Souza A.V., Liloia A, Soares M.P., Usheva A, Yamashita K and Bach F.H. Bilirubin: a natural inhibitor of vascular smooth muscle cell proliferation. Circulation 2005; 112:1030-1039.

8. Akamatsu Y, Haga M, Tyagi S.R., Yamashita K, Graca-Souza A.V., Ollinger R, Csizmadia E, May G.A., Ifedigbo E, Otterbein L.E., Bach F.H., and Soares M.P. Heme Oxygenase-1 derived carbon monoxide protects hearts from transplant associated ischiemia reperfusion injury. FASEB J. 2004; 18, 771-2

9. Otterbein L.E., Zuckerbraun B.S., Haga M, Liu F, Song R, Usheva A, Stachulak C, Bodyak N, Smith N.R., Cismadia E, Tyagi S.R., Akamatsu Y, Flavell R.J., Billiar T.R., Tzeng E, Bach F.H., Choi A.M.K., Soares M.P. Carbon monoxide suppresses arteriosclerotic lesions associated with chronic graft rejection and with balloon injury. Nature Med. 2003; 9, 183-90.

HONORS/AWARDS

2008-2012 Clinical Innovation Award (FAMRI- Medical Research Institute)

2008-2010 American Thoracic Society Research Award and Alpha-1-Foundation

2005-2006 National Research Service Award, Harvard University Medical School, and NIH

1998-1999 National Research Service Award, Harvard University Medical School, and NIH

1997-1998 National Research Service Award, Harvard University Medical School, and NIH

1981-1985 Junior/Senior Fellowship awards from Indian Council of Medical Research, New Delhi, India

REFERENCE (will be provided upon request)

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